Job ID = 6457710 SRX = SRX471891 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:53:33 prefetch.2.10.7: 1) Downloading 'SRR1170757'... 2020-06-21T11:53:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:21:38 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:21:38 prefetch.2.10.7: 1) 'SRR1170757' was downloaded successfully Read 163679304 spots for SRR1170757/SRR1170757.sra Written 163679304 spots for SRR1170757/SRR1170757.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:32:34 163679304 reads; of these: 163679304 (100.00%) were unpaired; of these: 8539027 (5.22%) aligned 0 times 33846382 (20.68%) aligned exactly 1 time 121293895 (74.10%) aligned >1 times 94.78% overall alignment rate Time searching: 01:32:34 Overall time: 01:32:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 64 files... [bam_rmdupse_core] 107351072 / 155140277 = 0.6920 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:29:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:29:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:29:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:29:55: 1000000 INFO @ Sun, 21 Jun 2020 23:30:00: 2000000 INFO @ Sun, 21 Jun 2020 23:30:06: 3000000 INFO @ Sun, 21 Jun 2020 23:30:11: 4000000 INFO @ Sun, 21 Jun 2020 23:30:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:30:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:30:20: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:30:20: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:30:22: 6000000 INFO @ Sun, 21 Jun 2020 23:30:26: 1000000 INFO @ Sun, 21 Jun 2020 23:30:28: 7000000 INFO @ Sun, 21 Jun 2020 23:30:32: 2000000 INFO @ Sun, 21 Jun 2020 23:30:34: 8000000 INFO @ Sun, 21 Jun 2020 23:30:38: 3000000 INFO @ Sun, 21 Jun 2020 23:30:40: 9000000 INFO @ Sun, 21 Jun 2020 23:30:44: 4000000 INFO @ Sun, 21 Jun 2020 23:30:46: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 23:30:49: 5000000 INFO @ Sun, 21 Jun 2020 23:30:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 23:30:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 23:30:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 23:30:52: 11000000 INFO @ Sun, 21 Jun 2020 23:30:55: 6000000 INFO @ Sun, 21 Jun 2020 23:30:56: 1000000 INFO @ Sun, 21 Jun 2020 23:30:58: 12000000 INFO @ Sun, 21 Jun 2020 23:31:01: 7000000 INFO @ Sun, 21 Jun 2020 23:31:02: 2000000 INFO @ Sun, 21 Jun 2020 23:31:04: 13000000 INFO @ Sun, 21 Jun 2020 23:31:07: 8000000 INFO @ Sun, 21 Jun 2020 23:31:08: 3000000 INFO @ Sun, 21 Jun 2020 23:31:10: 14000000 INFO @ Sun, 21 Jun 2020 23:31:13: 9000000 INFO @ Sun, 21 Jun 2020 23:31:14: 4000000 INFO @ Sun, 21 Jun 2020 23:31:16: 15000000 INFO @ Sun, 21 Jun 2020 23:31:19: 10000000 INFO @ Sun, 21 Jun 2020 23:31:19: 5000000 INFO @ Sun, 21 Jun 2020 23:31:22: 16000000 INFO @ Sun, 21 Jun 2020 23:31:25: 11000000 INFO @ Sun, 21 Jun 2020 23:31:25: 6000000 INFO @ Sun, 21 Jun 2020 23:31:27: 17000000 INFO @ Sun, 21 Jun 2020 23:31:31: 12000000 INFO @ Sun, 21 Jun 2020 23:31:31: 7000000 INFO @ Sun, 21 Jun 2020 23:31:33: 18000000 INFO @ Sun, 21 Jun 2020 23:31:37: 8000000 INFO @ Sun, 21 Jun 2020 23:31:37: 13000000 INFO @ Sun, 21 Jun 2020 23:31:39: 19000000 INFO @ Sun, 21 Jun 2020 23:31:43: 9000000 INFO @ Sun, 21 Jun 2020 23:31:43: 14000000 INFO @ Sun, 21 Jun 2020 23:31:45: 20000000 INFO @ Sun, 21 Jun 2020 23:31:49: 10000000 INFO @ Sun, 21 Jun 2020 23:31:49: 15000000 INFO @ Sun, 21 Jun 2020 23:31:51: 21000000 INFO @ Sun, 21 Jun 2020 23:31:54: 11000000 INFO @ Sun, 21 Jun 2020 23:31:55: 16000000 INFO @ Sun, 21 Jun 2020 23:31:57: 22000000 INFO @ Sun, 21 Jun 2020 23:32:00: 12000000 INFO @ Sun, 21 Jun 2020 23:32:01: 17000000 INFO @ Sun, 21 Jun 2020 23:32:03: 23000000 INFO @ Sun, 21 Jun 2020 23:32:06: 13000000 INFO @ Sun, 21 Jun 2020 23:32:07: 18000000 INFO @ Sun, 21 Jun 2020 23:32:10: 24000000 INFO @ Sun, 21 Jun 2020 23:32:13: 14000000 INFO @ Sun, 21 Jun 2020 23:32:13: 19000000 INFO @ Sun, 21 Jun 2020 23:32:16: 25000000 INFO @ Sun, 21 Jun 2020 23:32:19: 15000000 INFO @ Sun, 21 Jun 2020 23:32:19: 20000000 INFO @ Sun, 21 Jun 2020 23:32:22: 26000000 INFO @ Sun, 21 Jun 2020 23:32:25: 16000000 INFO @ Sun, 21 Jun 2020 23:32:25: 21000000 INFO @ Sun, 21 Jun 2020 23:32:28: 27000000 INFO @ Sun, 21 Jun 2020 23:32:31: 17000000 INFO @ Sun, 21 Jun 2020 23:32:31: 22000000 INFO @ Sun, 21 Jun 2020 23:32:35: 28000000 INFO @ Sun, 21 Jun 2020 23:32:37: 18000000 INFO @ Sun, 21 Jun 2020 23:32:37: 23000000 INFO @ Sun, 21 Jun 2020 23:32:41: 29000000 INFO @ Sun, 21 Jun 2020 23:32:43: 19000000 INFO @ Sun, 21 Jun 2020 23:32:43: 24000000 INFO @ Sun, 21 Jun 2020 23:32:47: 30000000 INFO @ Sun, 21 Jun 2020 23:32:49: 20000000 INFO @ Sun, 21 Jun 2020 23:32:49: 25000000 INFO @ Sun, 21 Jun 2020 23:32:53: 31000000 INFO @ Sun, 21 Jun 2020 23:32:55: 21000000 INFO @ Sun, 21 Jun 2020 23:32:55: 26000000 INFO @ Sun, 21 Jun 2020 23:32:59: 32000000 INFO @ Sun, 21 Jun 2020 23:33:01: 22000000 INFO @ Sun, 21 Jun 2020 23:33:01: 27000000 INFO @ Sun, 21 Jun 2020 23:33:05: 33000000 INFO @ Sun, 21 Jun 2020 23:33:07: 23000000 INFO @ Sun, 21 Jun 2020 23:33:07: 28000000 INFO @ Sun, 21 Jun 2020 23:33:11: 34000000 INFO @ Sun, 21 Jun 2020 23:33:13: 24000000 INFO @ Sun, 21 Jun 2020 23:33:13: 29000000 INFO @ Sun, 21 Jun 2020 23:33:17: 35000000 INFO @ Sun, 21 Jun 2020 23:33:19: 25000000 INFO @ Sun, 21 Jun 2020 23:33:19: 30000000 INFO @ Sun, 21 Jun 2020 23:33:23: 36000000 INFO @ Sun, 21 Jun 2020 23:33:25: 26000000 INFO @ Sun, 21 Jun 2020 23:33:25: 31000000 INFO @ Sun, 21 Jun 2020 23:33:29: 37000000 INFO @ Sun, 21 Jun 2020 23:33:31: 27000000 INFO @ Sun, 21 Jun 2020 23:33:31: 32000000 INFO @ Sun, 21 Jun 2020 23:33:35: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 23:33:37: 28000000 INFO @ Sun, 21 Jun 2020 23:33:37: 33000000 INFO @ Sun, 21 Jun 2020 23:33:41: 39000000 INFO @ Sun, 21 Jun 2020 23:33:43: 29000000 INFO @ Sun, 21 Jun 2020 23:33:43: 34000000 INFO @ Sun, 21 Jun 2020 23:33:47: 40000000 INFO @ Sun, 21 Jun 2020 23:33:49: 30000000 INFO @ Sun, 21 Jun 2020 23:33:49: 35000000 INFO @ Sun, 21 Jun 2020 23:33:53: 41000000 INFO @ Sun, 21 Jun 2020 23:33:55: 31000000 INFO @ Sun, 21 Jun 2020 23:33:55: 36000000 INFO @ Sun, 21 Jun 2020 23:33:59: 42000000 INFO @ Sun, 21 Jun 2020 23:34:01: 32000000 INFO @ Sun, 21 Jun 2020 23:34:01: 37000000 INFO @ Sun, 21 Jun 2020 23:34:05: 43000000 INFO @ Sun, 21 Jun 2020 23:34:07: 33000000 INFO @ Sun, 21 Jun 2020 23:34:07: 38000000 INFO @ Sun, 21 Jun 2020 23:34:11: 44000000 INFO @ Sun, 21 Jun 2020 23:34:13: 34000000 INFO @ Sun, 21 Jun 2020 23:34:13: 39000000 INFO @ Sun, 21 Jun 2020 23:34:18: 45000000 INFO @ Sun, 21 Jun 2020 23:34:19: 35000000 INFO @ Sun, 21 Jun 2020 23:34:20: 40000000 INFO @ Sun, 21 Jun 2020 23:34:24: 46000000 INFO @ Sun, 21 Jun 2020 23:34:25: 36000000 INFO @ Sun, 21 Jun 2020 23:34:26: 41000000 INFO @ Sun, 21 Jun 2020 23:34:31: 47000000 INFO @ Sun, 21 Jun 2020 23:34:31: 37000000 INFO @ Sun, 21 Jun 2020 23:34:32: 42000000 INFO @ Sun, 21 Jun 2020 23:34:36: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 23:34:36: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 23:34:36: #1 total tags in treatment: 47789205 INFO @ Sun, 21 Jun 2020 23:34:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:34:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:34:37: 38000000 INFO @ Sun, 21 Jun 2020 23:34:37: #1 tags after filtering in treatment: 47789205 INFO @ Sun, 21 Jun 2020 23:34:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:34:37: #1 finished! INFO @ Sun, 21 Jun 2020 23:34:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:34:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:34:38: 43000000 INFO @ Sun, 21 Jun 2020 23:34:40: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 23:34:40: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:34:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:34:43: 39000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 23:34:45: 44000000 INFO @ Sun, 21 Jun 2020 23:34:49: 40000000 INFO @ Sun, 21 Jun 2020 23:34:51: 45000000 INFO @ Sun, 21 Jun 2020 23:34:55: 41000000 INFO @ Sun, 21 Jun 2020 23:34:57: 46000000 INFO @ Sun, 21 Jun 2020 23:35:01: 42000000 INFO @ Sun, 21 Jun 2020 23:35:03: 47000000 INFO @ Sun, 21 Jun 2020 23:35:07: 43000000 INFO @ Sun, 21 Jun 2020 23:35:08: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 23:35:08: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 23:35:08: #1 total tags in treatment: 47789205 INFO @ Sun, 21 Jun 2020 23:35:08: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:35:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:35:09: #1 tags after filtering in treatment: 47789205 INFO @ Sun, 21 Jun 2020 23:35:09: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:35:09: #1 finished! INFO @ Sun, 21 Jun 2020 23:35:09: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:35:09: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:35:12: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 23:35:12: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:35:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 23:35:13: 44000000 INFO @ Sun, 21 Jun 2020 23:35:19: 45000000 INFO @ Sun, 21 Jun 2020 23:35:24: 46000000 INFO @ Sun, 21 Jun 2020 23:35:30: 47000000 INFO @ Sun, 21 Jun 2020 23:35:34: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 23:35:34: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 23:35:34: #1 total tags in treatment: 47789205 INFO @ Sun, 21 Jun 2020 23:35:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 23:35:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 23:35:35: #1 tags after filtering in treatment: 47789205 INFO @ Sun, 21 Jun 2020 23:35:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 23:35:35: #1 finished! INFO @ Sun, 21 Jun 2020 23:35:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 23:35:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 23:35:38: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 23:35:38: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 23:35:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX471891/SRX471891.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling