Job ID = 14167694 SRX = SRX4712956 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:03 60230563 reads; of these: 60230563 (100.00%) were unpaired; of these: 1720494 (2.86%) aligned 0 times 43164111 (71.66%) aligned exactly 1 time 15345958 (25.48%) aligned >1 times 97.14% overall alignment rate Time searching: 00:18:03 Overall time: 00:18:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 15877291 / 58510069 = 0.2714 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:37:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:37:16: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:37:16: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:37:23: 1000000 INFO @ Fri, 10 Dec 2021 13:37:30: 2000000 INFO @ Fri, 10 Dec 2021 13:37:37: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:37:44: 4000000 INFO @ Fri, 10 Dec 2021 13:37:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:37:45: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:37:45: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:37:52: 5000000 INFO @ Fri, 10 Dec 2021 13:37:52: 1000000 INFO @ Fri, 10 Dec 2021 13:38:00: 2000000 INFO @ Fri, 10 Dec 2021 13:38:00: 6000000 INFO @ Fri, 10 Dec 2021 13:38:07: 3000000 INFO @ Fri, 10 Dec 2021 13:38:08: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:38:14: 4000000 INFO @ Fri, 10 Dec 2021 13:38:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:38:15: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:38:15: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:38:16: 8000000 INFO @ Fri, 10 Dec 2021 13:38:22: 5000000 INFO @ Fri, 10 Dec 2021 13:38:23: 1000000 INFO @ Fri, 10 Dec 2021 13:38:24: 9000000 INFO @ Fri, 10 Dec 2021 13:38:29: 6000000 INFO @ Fri, 10 Dec 2021 13:38:30: 2000000 INFO @ Fri, 10 Dec 2021 13:38:33: 10000000 INFO @ Fri, 10 Dec 2021 13:38:37: 7000000 INFO @ Fri, 10 Dec 2021 13:38:38: 3000000 INFO @ Fri, 10 Dec 2021 13:38:41: 11000000 INFO @ Fri, 10 Dec 2021 13:38:44: 8000000 INFO @ Fri, 10 Dec 2021 13:38:45: 4000000 INFO @ Fri, 10 Dec 2021 13:38:49: 12000000 INFO @ Fri, 10 Dec 2021 13:38:52: 9000000 INFO @ Fri, 10 Dec 2021 13:38:53: 5000000 INFO @ Fri, 10 Dec 2021 13:38:57: 13000000 INFO @ Fri, 10 Dec 2021 13:38:59: 10000000 INFO @ Fri, 10 Dec 2021 13:39:00: 6000000 INFO @ Fri, 10 Dec 2021 13:39:05: 14000000 INFO @ Fri, 10 Dec 2021 13:39:06: 11000000 INFO @ Fri, 10 Dec 2021 13:39:07: 7000000 INFO @ Fri, 10 Dec 2021 13:39:13: 15000000 INFO @ Fri, 10 Dec 2021 13:39:14: 12000000 INFO @ Fri, 10 Dec 2021 13:39:15: 8000000 INFO @ Fri, 10 Dec 2021 13:39:20: 16000000 INFO @ Fri, 10 Dec 2021 13:39:21: 13000000 INFO @ Fri, 10 Dec 2021 13:39:22: 9000000 INFO @ Fri, 10 Dec 2021 13:39:28: 17000000 INFO @ Fri, 10 Dec 2021 13:39:29: 14000000 INFO @ Fri, 10 Dec 2021 13:39:30: 10000000 INFO @ Fri, 10 Dec 2021 13:39:36: 18000000 INFO @ Fri, 10 Dec 2021 13:39:36: 15000000 INFO @ Fri, 10 Dec 2021 13:39:37: 11000000 INFO @ Fri, 10 Dec 2021 13:39:44: 16000000 INFO @ Fri, 10 Dec 2021 13:39:44: 19000000 INFO @ Fri, 10 Dec 2021 13:39:44: 12000000 INFO @ Fri, 10 Dec 2021 13:39:51: 17000000 INFO @ Fri, 10 Dec 2021 13:39:52: 13000000 INFO @ Fri, 10 Dec 2021 13:39:52: 20000000 INFO @ Fri, 10 Dec 2021 13:39:58: 18000000 INFO @ Fri, 10 Dec 2021 13:39:59: 14000000 INFO @ Fri, 10 Dec 2021 13:40:00: 21000000 INFO @ Fri, 10 Dec 2021 13:40:06: 19000000 INFO @ Fri, 10 Dec 2021 13:40:07: 15000000 INFO @ Fri, 10 Dec 2021 13:40:07: 22000000 INFO @ Fri, 10 Dec 2021 13:40:13: 20000000 INFO @ Fri, 10 Dec 2021 13:40:14: 16000000 INFO @ Fri, 10 Dec 2021 13:40:15: 23000000 INFO @ Fri, 10 Dec 2021 13:40:20: 21000000 INFO @ Fri, 10 Dec 2021 13:40:22: 17000000 INFO @ Fri, 10 Dec 2021 13:40:23: 24000000 INFO @ Fri, 10 Dec 2021 13:40:28: 22000000 INFO @ Fri, 10 Dec 2021 13:40:29: 18000000 INFO @ Fri, 10 Dec 2021 13:40:30: 25000000 INFO @ Fri, 10 Dec 2021 13:40:35: 23000000 INFO @ Fri, 10 Dec 2021 13:40:36: 19000000 INFO @ Fri, 10 Dec 2021 13:40:38: 26000000 INFO @ Fri, 10 Dec 2021 13:40:42: 24000000 INFO @ Fri, 10 Dec 2021 13:40:43: 20000000 INFO @ Fri, 10 Dec 2021 13:40:46: 27000000 INFO @ Fri, 10 Dec 2021 13:40:49: 25000000 INFO @ Fri, 10 Dec 2021 13:40:50: 21000000 INFO @ Fri, 10 Dec 2021 13:40:54: 28000000 INFO @ Fri, 10 Dec 2021 13:40:57: 26000000 INFO @ Fri, 10 Dec 2021 13:40:58: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:41:02: 29000000 INFO @ Fri, 10 Dec 2021 13:41:04: 27000000 INFO @ Fri, 10 Dec 2021 13:41:05: 23000000 INFO @ Fri, 10 Dec 2021 13:41:10: 30000000 INFO @ Fri, 10 Dec 2021 13:41:11: 28000000 INFO @ Fri, 10 Dec 2021 13:41:12: 24000000 INFO @ Fri, 10 Dec 2021 13:41:17: 31000000 INFO @ Fri, 10 Dec 2021 13:41:18: 29000000 INFO @ Fri, 10 Dec 2021 13:41:19: 25000000 INFO @ Fri, 10 Dec 2021 13:41:25: 32000000 INFO @ Fri, 10 Dec 2021 13:41:25: 30000000 INFO @ Fri, 10 Dec 2021 13:41:27: 26000000 INFO @ Fri, 10 Dec 2021 13:41:33: 31000000 INFO @ Fri, 10 Dec 2021 13:41:33: 33000000 INFO @ Fri, 10 Dec 2021 13:41:34: 27000000 INFO @ Fri, 10 Dec 2021 13:41:40: 32000000 INFO @ Fri, 10 Dec 2021 13:41:42: 28000000 INFO @ Fri, 10 Dec 2021 13:41:42: 34000000 INFO @ Fri, 10 Dec 2021 13:41:47: 33000000 INFO @ Fri, 10 Dec 2021 13:41:49: 29000000 INFO @ Fri, 10 Dec 2021 13:41:50: 35000000 INFO @ Fri, 10 Dec 2021 13:41:55: 34000000 INFO @ Fri, 10 Dec 2021 13:41:56: 30000000 INFO @ Fri, 10 Dec 2021 13:41:58: 36000000 INFO @ Fri, 10 Dec 2021 13:42:02: 35000000 INFO @ Fri, 10 Dec 2021 13:42:03: 31000000 INFO @ Fri, 10 Dec 2021 13:42:06: 37000000 INFO @ Fri, 10 Dec 2021 13:42:09: 36000000 INFO @ Fri, 10 Dec 2021 13:42:10: 32000000 INFO @ Fri, 10 Dec 2021 13:42:14: 38000000 INFO @ Fri, 10 Dec 2021 13:42:17: 37000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:42:17: 33000000 INFO @ Fri, 10 Dec 2021 13:42:23: 39000000 INFO @ Fri, 10 Dec 2021 13:42:24: 38000000 INFO @ Fri, 10 Dec 2021 13:42:25: 34000000 INFO @ Fri, 10 Dec 2021 13:42:31: 40000000 INFO @ Fri, 10 Dec 2021 13:42:31: 39000000 INFO @ Fri, 10 Dec 2021 13:42:33: 35000000 INFO @ Fri, 10 Dec 2021 13:42:39: 40000000 INFO @ Fri, 10 Dec 2021 13:42:39: 41000000 INFO @ Fri, 10 Dec 2021 13:42:40: 36000000 INFO @ Fri, 10 Dec 2021 13:42:46: 41000000 INFO @ Fri, 10 Dec 2021 13:42:48: 37000000 INFO @ Fri, 10 Dec 2021 13:42:48: 42000000 INFO @ Fri, 10 Dec 2021 13:42:53: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 13:42:53: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 13:42:53: #1 total tags in treatment: 42632778 INFO @ Fri, 10 Dec 2021 13:42:53: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:42:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:42:54: 42000000 INFO @ Fri, 10 Dec 2021 13:42:54: #1 tags after filtering in treatment: 42632771 INFO @ Fri, 10 Dec 2021 13:42:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:42:54: #1 finished! INFO @ Fri, 10 Dec 2021 13:42:54: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:42:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:42:55: 38000000 INFO @ Fri, 10 Dec 2021 13:42:57: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 13:42:57: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:42:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:42:59: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 13:42:59: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 13:42:59: #1 total tags in treatment: 42632778 INFO @ Fri, 10 Dec 2021 13:42:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:42:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:43:00: #1 tags after filtering in treatment: 42632771 INFO @ Fri, 10 Dec 2021 13:43:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:43:00: #1 finished! INFO @ Fri, 10 Dec 2021 13:43:00: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:43:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:43:01: 39000000 INFO @ Fri, 10 Dec 2021 13:43:02: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 13:43:02: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:43:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:43:07: 40000000 INFO @ Fri, 10 Dec 2021 13:43:13: 41000000 INFO @ Fri, 10 Dec 2021 13:43:19: 42000000 INFO @ Fri, 10 Dec 2021 13:43:23: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 13:43:23: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 13:43:23: #1 total tags in treatment: 42632778 INFO @ Fri, 10 Dec 2021 13:43:23: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:43:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:43:24: #1 tags after filtering in treatment: 42632771 INFO @ Fri, 10 Dec 2021 13:43:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:43:24: #1 finished! INFO @ Fri, 10 Dec 2021 13:43:24: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:43:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:43:26: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 13:43:26: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 13:43:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4712956/SRX4712956.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling