Job ID = 14167635 SRX = SRX4712952 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:29 45849454 reads; of these: 45849454 (100.00%) were unpaired; of these: 2535495 (5.53%) aligned 0 times 33963733 (74.08%) aligned exactly 1 time 9350226 (20.39%) aligned >1 times 94.47% overall alignment rate Time searching: 00:11:29 Overall time: 00:11:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 28540458 / 43313959 = 0.6589 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:12:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:12:38: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:12:38: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:12:43: 1000000 INFO @ Fri, 10 Dec 2021 13:12:48: 2000000 INFO @ Fri, 10 Dec 2021 13:12:54: 3000000 INFO @ Fri, 10 Dec 2021 13:12:59: 4000000 INFO @ Fri, 10 Dec 2021 13:13:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:13:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:13:08: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:13:08: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:13:10: 6000000 INFO @ Fri, 10 Dec 2021 13:13:15: 1000000 INFO @ Fri, 10 Dec 2021 13:13:16: 7000000 INFO @ Fri, 10 Dec 2021 13:13:22: 2000000 INFO @ Fri, 10 Dec 2021 13:13:23: 8000000 INFO @ Fri, 10 Dec 2021 13:13:29: 9000000 INFO @ Fri, 10 Dec 2021 13:13:29: 3000000 INFO @ Fri, 10 Dec 2021 13:13:36: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:13:37: 4000000 INFO @ Fri, 10 Dec 2021 13:13:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:13:38: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:13:38: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:13:42: 11000000 INFO @ Fri, 10 Dec 2021 13:13:44: 5000000 INFO @ Fri, 10 Dec 2021 13:13:44: 1000000 INFO @ Fri, 10 Dec 2021 13:13:49: 12000000 INFO @ Fri, 10 Dec 2021 13:13:51: 2000000 INFO @ Fri, 10 Dec 2021 13:13:51: 6000000 INFO @ Fri, 10 Dec 2021 13:13:55: 13000000 INFO @ Fri, 10 Dec 2021 13:13:57: 3000000 INFO @ Fri, 10 Dec 2021 13:13:58: 7000000 INFO @ Fri, 10 Dec 2021 13:14:02: 14000000 INFO @ Fri, 10 Dec 2021 13:14:04: 4000000 INFO @ Fri, 10 Dec 2021 13:14:06: 8000000 INFO @ Fri, 10 Dec 2021 13:14:07: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 13:14:07: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 13:14:07: #1 total tags in treatment: 14773501 INFO @ Fri, 10 Dec 2021 13:14:07: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:14:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:14:08: #1 tags after filtering in treatment: 14773499 INFO @ Fri, 10 Dec 2021 13:14:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:14:08: #1 finished! INFO @ Fri, 10 Dec 2021 13:14:08: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:14:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:14:09: #2 number of paired peaks: 1322 INFO @ Fri, 10 Dec 2021 13:14:09: start model_add_line... INFO @ Fri, 10 Dec 2021 13:14:09: start X-correlation... INFO @ Fri, 10 Dec 2021 13:14:09: end of X-cor INFO @ Fri, 10 Dec 2021 13:14:09: #2 finished! INFO @ Fri, 10 Dec 2021 13:14:09: #2 predicted fragment length is 118 bps INFO @ Fri, 10 Dec 2021 13:14:09: #2 alternative fragment length(s) may be 118 bps INFO @ Fri, 10 Dec 2021 13:14:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.05_model.r INFO @ Fri, 10 Dec 2021 13:14:09: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:14:09: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:14:10: 5000000 INFO @ Fri, 10 Dec 2021 13:14:13: 9000000 INFO @ Fri, 10 Dec 2021 13:14:17: 6000000 INFO @ Fri, 10 Dec 2021 13:14:20: 10000000 INFO @ Fri, 10 Dec 2021 13:14:23: 7000000 INFO @ Fri, 10 Dec 2021 13:14:27: 11000000 INFO @ Fri, 10 Dec 2021 13:14:30: 8000000 INFO @ Fri, 10 Dec 2021 13:14:35: 12000000 INFO @ Fri, 10 Dec 2021 13:14:36: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:14:40: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:14:42: 13000000 INFO @ Fri, 10 Dec 2021 13:14:43: 10000000 INFO @ Fri, 10 Dec 2021 13:14:49: 11000000 INFO @ Fri, 10 Dec 2021 13:14:49: 14000000 INFO @ Fri, 10 Dec 2021 13:14:55: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 13:14:55: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 13:14:55: #1 total tags in treatment: 14773501 INFO @ Fri, 10 Dec 2021 13:14:55: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:14:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:14:55: #1 tags after filtering in treatment: 14773499 INFO @ Fri, 10 Dec 2021 13:14:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:14:55: #1 finished! INFO @ Fri, 10 Dec 2021 13:14:55: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:14:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:14:56: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.05_peaks.xls INFO @ Fri, 10 Dec 2021 13:14:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:14:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.05_summits.bed INFO @ Fri, 10 Dec 2021 13:14:56: Done! INFO @ Fri, 10 Dec 2021 13:14:56: 12000000 pass1 - making usageList (833 chroms): 2 millis pass2 - checking and writing primary data (7682 records, 4 fields): 28 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:14:56: #2 number of paired peaks: 1322 INFO @ Fri, 10 Dec 2021 13:14:56: start model_add_line... INFO @ Fri, 10 Dec 2021 13:14:56: start X-correlation... INFO @ Fri, 10 Dec 2021 13:14:56: end of X-cor INFO @ Fri, 10 Dec 2021 13:14:56: #2 finished! INFO @ Fri, 10 Dec 2021 13:14:56: #2 predicted fragment length is 118 bps INFO @ Fri, 10 Dec 2021 13:14:56: #2 alternative fragment length(s) may be 118 bps INFO @ Fri, 10 Dec 2021 13:14:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.10_model.r INFO @ Fri, 10 Dec 2021 13:14:56: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:14:56: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:15:02: 13000000 INFO @ Fri, 10 Dec 2021 13:15:08: 14000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:15:12: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 13:15:12: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 13:15:12: #1 total tags in treatment: 14773501 INFO @ Fri, 10 Dec 2021 13:15:12: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:15:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:15:13: #1 tags after filtering in treatment: 14773499 INFO @ Fri, 10 Dec 2021 13:15:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:15:13: #1 finished! INFO @ Fri, 10 Dec 2021 13:15:13: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:15:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:15:14: #2 number of paired peaks: 1322 INFO @ Fri, 10 Dec 2021 13:15:14: start model_add_line... INFO @ Fri, 10 Dec 2021 13:15:14: start X-correlation... INFO @ Fri, 10 Dec 2021 13:15:14: end of X-cor INFO @ Fri, 10 Dec 2021 13:15:14: #2 finished! INFO @ Fri, 10 Dec 2021 13:15:14: #2 predicted fragment length is 118 bps INFO @ Fri, 10 Dec 2021 13:15:14: #2 alternative fragment length(s) may be 118 bps INFO @ Fri, 10 Dec 2021 13:15:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.20_model.r INFO @ Fri, 10 Dec 2021 13:15:14: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:15:14: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:15:27: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:15:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.10_peaks.xls INFO @ Fri, 10 Dec 2021 13:15:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:15:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.10_summits.bed INFO @ Fri, 10 Dec 2021 13:15:42: Done! pass1 - making usageList (683 chroms): 2 millis pass2 - checking and writing primary data (5698 records, 4 fields): 23 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:15:45: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:16:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.20_peaks.xls INFO @ Fri, 10 Dec 2021 13:16:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:16:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX4712952/SRX4712952.20_summits.bed INFO @ Fri, 10 Dec 2021 13:16:00: Done! pass1 - making usageList (575 chroms): 1 millis pass2 - checking and writing primary data (4014 records, 4 fields): 19 millis CompletedMACS2peakCalling