Job ID = 14167576 SRX = SRX4712946 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:34 24841699 reads; of these: 24841699 (100.00%) were unpaired; of these: 1511652 (6.09%) aligned 0 times 18100427 (72.86%) aligned exactly 1 time 5229620 (21.05%) aligned >1 times 93.91% overall alignment rate Time searching: 00:08:35 Overall time: 00:08:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8972799 / 23330047 = 0.3846 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:55:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:55:07: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:55:07: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:55:16: 1000000 INFO @ Fri, 10 Dec 2021 12:55:25: 2000000 INFO @ Fri, 10 Dec 2021 12:55:32: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:55:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:55:37: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:55:37: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:55:40: 4000000 INFO @ Fri, 10 Dec 2021 12:55:44: 1000000 INFO @ Fri, 10 Dec 2021 12:55:48: 5000000 INFO @ Fri, 10 Dec 2021 12:55:51: 2000000 INFO @ Fri, 10 Dec 2021 12:55:56: 6000000 INFO @ Fri, 10 Dec 2021 12:55:58: 3000000 INFO @ Fri, 10 Dec 2021 12:56:04: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:56:05: 4000000 INFO @ Fri, 10 Dec 2021 12:56:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:56:07: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:56:07: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:56:12: 5000000 INFO @ Fri, 10 Dec 2021 12:56:12: 8000000 INFO @ Fri, 10 Dec 2021 12:56:16: 1000000 INFO @ Fri, 10 Dec 2021 12:56:19: 6000000 INFO @ Fri, 10 Dec 2021 12:56:20: 9000000 INFO @ Fri, 10 Dec 2021 12:56:25: 2000000 INFO @ Fri, 10 Dec 2021 12:56:26: 7000000 INFO @ Fri, 10 Dec 2021 12:56:27: 10000000 INFO @ Fri, 10 Dec 2021 12:56:32: 8000000 INFO @ Fri, 10 Dec 2021 12:56:34: 3000000 INFO @ Fri, 10 Dec 2021 12:56:35: 11000000 INFO @ Fri, 10 Dec 2021 12:56:39: 9000000 INFO @ Fri, 10 Dec 2021 12:56:43: 4000000 INFO @ Fri, 10 Dec 2021 12:56:43: 12000000 INFO @ Fri, 10 Dec 2021 12:56:46: 10000000 INFO @ Fri, 10 Dec 2021 12:56:51: 13000000 INFO @ Fri, 10 Dec 2021 12:56:52: 5000000 INFO @ Fri, 10 Dec 2021 12:56:53: 11000000 INFO @ Fri, 10 Dec 2021 12:56:59: 14000000 INFO @ Fri, 10 Dec 2021 12:57:01: 6000000 INFO @ Fri, 10 Dec 2021 12:57:01: 12000000 INFO @ Fri, 10 Dec 2021 12:57:03: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 12:57:03: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 12:57:03: #1 total tags in treatment: 14357248 INFO @ Fri, 10 Dec 2021 12:57:03: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:57:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:57:03: #1 tags after filtering in treatment: 14357245 INFO @ Fri, 10 Dec 2021 12:57:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:57:03: #1 finished! INFO @ Fri, 10 Dec 2021 12:57:03: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:57:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:57:05: #2 number of paired peaks: 1126 INFO @ Fri, 10 Dec 2021 12:57:05: start model_add_line... INFO @ Fri, 10 Dec 2021 12:57:05: start X-correlation... INFO @ Fri, 10 Dec 2021 12:57:05: end of X-cor INFO @ Fri, 10 Dec 2021 12:57:05: #2 finished! INFO @ Fri, 10 Dec 2021 12:57:05: #2 predicted fragment length is 143 bps INFO @ Fri, 10 Dec 2021 12:57:05: #2 alternative fragment length(s) may be 143 bps INFO @ Fri, 10 Dec 2021 12:57:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.05_model.r INFO @ Fri, 10 Dec 2021 12:57:05: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:57:05: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:57:08: 13000000 INFO @ Fri, 10 Dec 2021 12:57:09: 7000000 INFO @ Fri, 10 Dec 2021 12:57:15: 14000000 INFO @ Fri, 10 Dec 2021 12:57:18: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 12:57:18: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 12:57:18: #1 total tags in treatment: 14357248 INFO @ Fri, 10 Dec 2021 12:57:18: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:57:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:57:18: 8000000 INFO @ Fri, 10 Dec 2021 12:57:18: #1 tags after filtering in treatment: 14357245 INFO @ Fri, 10 Dec 2021 12:57:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:57:18: #1 finished! INFO @ Fri, 10 Dec 2021 12:57:18: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:57:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:57:20: #2 number of paired peaks: 1126 INFO @ Fri, 10 Dec 2021 12:57:20: start model_add_line... INFO @ Fri, 10 Dec 2021 12:57:20: start X-correlation... INFO @ Fri, 10 Dec 2021 12:57:20: end of X-cor INFO @ Fri, 10 Dec 2021 12:57:20: #2 finished! INFO @ Fri, 10 Dec 2021 12:57:20: #2 predicted fragment length is 143 bps INFO @ Fri, 10 Dec 2021 12:57:20: #2 alternative fragment length(s) may be 143 bps INFO @ Fri, 10 Dec 2021 12:57:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.10_model.r INFO @ Fri, 10 Dec 2021 12:57:20: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:57:20: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:57:27: 9000000 INFO @ Fri, 10 Dec 2021 12:57:35: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:57:43: 11000000 INFO @ Fri, 10 Dec 2021 12:57:49: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:57:53: 12000000 INFO @ Fri, 10 Dec 2021 12:58:01: 13000000 INFO @ Fri, 10 Dec 2021 12:58:02: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:58:09: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.05_peaks.xls INFO @ Fri, 10 Dec 2021 12:58:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:58:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.05_summits.bed INFO @ Fri, 10 Dec 2021 12:58:09: Done! pass1 - making usageList (675 chroms): 3 millis pass2 - checking and writing primary data (7578 records, 4 fields): 56 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:58:10: 14000000 INFO @ Fri, 10 Dec 2021 12:58:13: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 12:58:13: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 12:58:13: #1 total tags in treatment: 14357248 INFO @ Fri, 10 Dec 2021 12:58:13: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:58:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:58:14: #1 tags after filtering in treatment: 14357245 INFO @ Fri, 10 Dec 2021 12:58:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:58:14: #1 finished! INFO @ Fri, 10 Dec 2021 12:58:14: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:58:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:58:16: #2 number of paired peaks: 1126 INFO @ Fri, 10 Dec 2021 12:58:16: start model_add_line... INFO @ Fri, 10 Dec 2021 12:58:16: start X-correlation... INFO @ Fri, 10 Dec 2021 12:58:16: end of X-cor INFO @ Fri, 10 Dec 2021 12:58:16: #2 finished! INFO @ Fri, 10 Dec 2021 12:58:16: #2 predicted fragment length is 143 bps INFO @ Fri, 10 Dec 2021 12:58:16: #2 alternative fragment length(s) may be 143 bps INFO @ Fri, 10 Dec 2021 12:58:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.20_model.r INFO @ Fri, 10 Dec 2021 12:58:16: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:58:16: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 12:58:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.10_peaks.xls INFO @ Fri, 10 Dec 2021 12:58:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:58:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.10_summits.bed INFO @ Fri, 10 Dec 2021 12:58:21: Done! pass1 - making usageList (588 chroms): 4 millis pass2 - checking and writing primary data (5883 records, 4 fields): 31 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:58:58: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:59:17: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.20_peaks.xls INFO @ Fri, 10 Dec 2021 12:59:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:59:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX4712946/SRX4712946.20_summits.bed INFO @ Fri, 10 Dec 2021 12:59:17: Done! pass1 - making usageList (461 chroms): 2 millis pass2 - checking and writing primary data (4126 records, 4 fields): 24 millis CompletedMACS2peakCalling