Job ID = 14167583 SRX = SRX4712943 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:39 44240601 reads; of these: 44240601 (100.00%) were unpaired; of these: 1785997 (4.04%) aligned 0 times 33623628 (76.00%) aligned exactly 1 time 8830976 (19.96%) aligned >1 times 95.96% overall alignment rate Time searching: 00:13:39 Overall time: 00:13:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 26277262 / 42454604 = 0.6189 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:11:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:11:18: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:11:18: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:11:25: 1000000 INFO @ Fri, 10 Dec 2021 13:11:32: 2000000 INFO @ Fri, 10 Dec 2021 13:11:39: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:11:46: 4000000 INFO @ Fri, 10 Dec 2021 13:11:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:11:50: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:11:50: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:11:55: 5000000 INFO @ Fri, 10 Dec 2021 13:11:58: 1000000 INFO @ Fri, 10 Dec 2021 13:12:02: 6000000 INFO @ Fri, 10 Dec 2021 13:12:05: 2000000 INFO @ Fri, 10 Dec 2021 13:12:09: 7000000 INFO @ Fri, 10 Dec 2021 13:12:12: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:12:16: 8000000 INFO @ Fri, 10 Dec 2021 13:12:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:12:19: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:12:19: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:12:20: 4000000 INFO @ Fri, 10 Dec 2021 13:12:23: 9000000 INFO @ Fri, 10 Dec 2021 13:12:26: 1000000 INFO @ Fri, 10 Dec 2021 13:12:27: 5000000 INFO @ Fri, 10 Dec 2021 13:12:31: 10000000 INFO @ Fri, 10 Dec 2021 13:12:34: 2000000 INFO @ Fri, 10 Dec 2021 13:12:35: 6000000 INFO @ Fri, 10 Dec 2021 13:12:38: 11000000 INFO @ Fri, 10 Dec 2021 13:12:42: 3000000 INFO @ Fri, 10 Dec 2021 13:12:42: 7000000 INFO @ Fri, 10 Dec 2021 13:12:46: 12000000 INFO @ Fri, 10 Dec 2021 13:12:49: 4000000 INFO @ Fri, 10 Dec 2021 13:12:50: 8000000 INFO @ Fri, 10 Dec 2021 13:12:55: 13000000 INFO @ Fri, 10 Dec 2021 13:12:57: 5000000 INFO @ Fri, 10 Dec 2021 13:12:58: 9000000 INFO @ Fri, 10 Dec 2021 13:13:02: 14000000 INFO @ Fri, 10 Dec 2021 13:13:04: 6000000 INFO @ Fri, 10 Dec 2021 13:13:05: 10000000 INFO @ Fri, 10 Dec 2021 13:13:10: 15000000 INFO @ Fri, 10 Dec 2021 13:13:13: 7000000 INFO @ Fri, 10 Dec 2021 13:13:14: 11000000 INFO @ Fri, 10 Dec 2021 13:13:18: 16000000 INFO @ Fri, 10 Dec 2021 13:13:21: 12000000 INFO @ Fri, 10 Dec 2021 13:13:21: 8000000 INFO @ Fri, 10 Dec 2021 13:13:22: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 13:13:22: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 13:13:22: #1 total tags in treatment: 16177342 INFO @ Fri, 10 Dec 2021 13:13:22: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:13:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:13:23: #1 tags after filtering in treatment: 16177342 INFO @ Fri, 10 Dec 2021 13:13:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:13:24: #1 finished! INFO @ Fri, 10 Dec 2021 13:13:24: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:13:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:13:25: #2 number of paired peaks: 2531 INFO @ Fri, 10 Dec 2021 13:13:25: start model_add_line... INFO @ Fri, 10 Dec 2021 13:13:25: start X-correlation... INFO @ Fri, 10 Dec 2021 13:13:26: end of X-cor INFO @ Fri, 10 Dec 2021 13:13:26: #2 finished! INFO @ Fri, 10 Dec 2021 13:13:26: #2 predicted fragment length is 165 bps INFO @ Fri, 10 Dec 2021 13:13:26: #2 alternative fragment length(s) may be 165 bps INFO @ Fri, 10 Dec 2021 13:13:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.05_model.r INFO @ Fri, 10 Dec 2021 13:13:26: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:13:26: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:13:29: 9000000 INFO @ Fri, 10 Dec 2021 13:13:30: 13000000 INFO @ Fri, 10 Dec 2021 13:13:36: 10000000 INFO @ Fri, 10 Dec 2021 13:13:39: 14000000 INFO @ Fri, 10 Dec 2021 13:13:45: 11000000 INFO @ Fri, 10 Dec 2021 13:13:46: 15000000 INFO @ Fri, 10 Dec 2021 13:13:52: 12000000 INFO @ Fri, 10 Dec 2021 13:13:55: 16000000 INFO @ Fri, 10 Dec 2021 13:13:58: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 13:13:58: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 13:13:58: #1 total tags in treatment: 16177342 INFO @ Fri, 10 Dec 2021 13:13:58: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:13:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:14:00: #1 tags after filtering in treatment: 16177342 INFO @ Fri, 10 Dec 2021 13:14:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:14:00: #1 finished! INFO @ Fri, 10 Dec 2021 13:14:00: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:14:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:14:01: #2 number of paired peaks: 2531 INFO @ Fri, 10 Dec 2021 13:14:02: start model_add_line... INFO @ Fri, 10 Dec 2021 13:14:02: start X-correlation... INFO @ Fri, 10 Dec 2021 13:14:02: 13000000 INFO @ Fri, 10 Dec 2021 13:14:02: end of X-cor INFO @ Fri, 10 Dec 2021 13:14:02: #2 finished! INFO @ Fri, 10 Dec 2021 13:14:02: #2 predicted fragment length is 165 bps INFO @ Fri, 10 Dec 2021 13:14:02: #2 alternative fragment length(s) may be 165 bps INFO @ Fri, 10 Dec 2021 13:14:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.10_model.r INFO @ Fri, 10 Dec 2021 13:14:02: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:14:02: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:14:08: 14000000 INFO @ Fri, 10 Dec 2021 13:14:14: 15000000 INFO @ Fri, 10 Dec 2021 13:14:22: 16000000 INFO @ Fri, 10 Dec 2021 13:14:23: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 13:14:23: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 13:14:23: #1 total tags in treatment: 16177342 INFO @ Fri, 10 Dec 2021 13:14:23: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:14:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:14:24: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:14:25: #1 tags after filtering in treatment: 16177342 INFO @ Fri, 10 Dec 2021 13:14:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 13:14:25: #1 finished! INFO @ Fri, 10 Dec 2021 13:14:25: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:14:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:14:27: #2 number of paired peaks: 2531 INFO @ Fri, 10 Dec 2021 13:14:27: start model_add_line... INFO @ Fri, 10 Dec 2021 13:14:27: start X-correlation... INFO @ Fri, 10 Dec 2021 13:14:27: end of X-cor INFO @ Fri, 10 Dec 2021 13:14:27: #2 finished! INFO @ Fri, 10 Dec 2021 13:14:27: #2 predicted fragment length is 165 bps INFO @ Fri, 10 Dec 2021 13:14:27: #2 alternative fragment length(s) may be 165 bps INFO @ Fri, 10 Dec 2021 13:14:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.20_model.r INFO @ Fri, 10 Dec 2021 13:14:27: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:14:27: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:14:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.05_peaks.xls INFO @ Fri, 10 Dec 2021 13:14:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:14:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.05_summits.bed INFO @ Fri, 10 Dec 2021 13:14:49: Done! pass1 - making usageList (800 chroms): 3 millis pass2 - checking and writing primary data (10614 records, 4 fields): 62 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:14:57: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:15:12: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:15:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.10_peaks.xls INFO @ Fri, 10 Dec 2021 13:15:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:15:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.10_summits.bed INFO @ Fri, 10 Dec 2021 13:15:26: Done! pass1 - making usageList (678 chroms): 2 millis pass2 - checking and writing primary data (8327 records, 4 fields): 53 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:15:40: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.20_peaks.xls INFO @ Fri, 10 Dec 2021 13:15:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:15:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX4712943/SRX4712943.20_summits.bed INFO @ Fri, 10 Dec 2021 13:15:40: Done! pass1 - making usageList (577 chroms): 3 millis pass2 - checking and writing primary data (6442 records, 4 fields): 43 millis CompletedMACS2peakCalling