Job ID = 6457664 SRX = SRX467065 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:52:03 prefetch.2.10.7: 1) Downloading 'SRR1164489'... 2020-06-21T11:52:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:52:12 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:52:12 prefetch.2.10.7: 'SRR1164489' is valid 2020-06-21T11:52:12 prefetch.2.10.7: 1) 'SRR1164489' was downloaded successfully Read 7340 spots for SRR1164489/SRR1164489.sra Written 7340 spots for SRR1164489/SRR1164489.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:00:00 7340 reads; of these: 7340 (100.00%) were unpaired; of these: 1914 (26.08%) aligned 0 times 4550 (61.99%) aligned exactly 1 time 876 (11.93%) aligned >1 times 73.92% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1118 / 5426 = 0.2060 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:52:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:52:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:52:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:52:45: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:52:45: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:52:45: #1 total tags in treatment: 4308 INFO @ Sun, 21 Jun 2020 20:52:45: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:52:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:52:45: #1 tags after filtering in treatment: 4167 INFO @ Sun, 21 Jun 2020 20:52:45: #1 Redundant rate of treatment: 0.03 INFO @ Sun, 21 Jun 2020 20:52:45: #1 finished! INFO @ Sun, 21 Jun 2020 20:52:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:52:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:52:45: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 20:52:45: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:52:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:53:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:53:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:53:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:53:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:53:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:53:15: #1 total tags in treatment: 4308 INFO @ Sun, 21 Jun 2020 20:53:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:53:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:53:15: #1 tags after filtering in treatment: 4167 INFO @ Sun, 21 Jun 2020 20:53:15: #1 Redundant rate of treatment: 0.03 INFO @ Sun, 21 Jun 2020 20:53:15: #1 finished! INFO @ Sun, 21 Jun 2020 20:53:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:53:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:53:15: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 20:53:15: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:53:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:53:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:53:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:53:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:53:45: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:53:45: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:53:45: #1 total tags in treatment: 4308 INFO @ Sun, 21 Jun 2020 20:53:45: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:53:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:53:45: #1 tags after filtering in treatment: 4167 INFO @ Sun, 21 Jun 2020 20:53:45: #1 Redundant rate of treatment: 0.03 INFO @ Sun, 21 Jun 2020 20:53:45: #1 finished! INFO @ Sun, 21 Jun 2020 20:53:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:53:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:53:45: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 20:53:45: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:53:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467065/SRX467065.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling