Job ID = 6457657 SRX = SRX467059 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:52:33 prefetch.2.10.7: 1) Downloading 'SRR1164483'... 2020-06-21T11:52:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:56:56 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:56:56 prefetch.2.10.7: 1) 'SRR1164483' was downloaded successfully Read 29206592 spots for SRR1164483/SRR1164483.sra Written 29206592 spots for SRR1164483/SRR1164483.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:07 29206592 reads; of these: 29206592 (100.00%) were unpaired; of these: 1045798 (3.58%) aligned 0 times 22388171 (76.65%) aligned exactly 1 time 5772623 (19.76%) aligned >1 times 96.42% overall alignment rate Time searching: 00:08:07 Overall time: 00:08:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6658712 / 28160794 = 0.2365 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:12:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:12:36: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:12:36: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:12:42: 1000000 INFO @ Sun, 21 Jun 2020 21:12:48: 2000000 INFO @ Sun, 21 Jun 2020 21:12:54: 3000000 INFO @ Sun, 21 Jun 2020 21:13:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:13:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:13:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:13:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:13:06: 5000000 INFO @ Sun, 21 Jun 2020 21:13:12: 1000000 INFO @ Sun, 21 Jun 2020 21:13:13: 6000000 INFO @ Sun, 21 Jun 2020 21:13:17: 2000000 INFO @ Sun, 21 Jun 2020 21:13:19: 7000000 INFO @ Sun, 21 Jun 2020 21:13:23: 3000000 INFO @ Sun, 21 Jun 2020 21:13:26: 8000000 INFO @ Sun, 21 Jun 2020 21:13:29: 4000000 INFO @ Sun, 21 Jun 2020 21:13:32: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:13:35: 5000000 INFO @ Sun, 21 Jun 2020 21:13:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:13:36: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:13:36: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:13:38: 10000000 INFO @ Sun, 21 Jun 2020 21:13:41: 6000000 INFO @ Sun, 21 Jun 2020 21:13:42: 1000000 INFO @ Sun, 21 Jun 2020 21:13:45: 11000000 INFO @ Sun, 21 Jun 2020 21:13:48: 7000000 INFO @ Sun, 21 Jun 2020 21:13:49: 2000000 INFO @ Sun, 21 Jun 2020 21:13:52: 12000000 INFO @ Sun, 21 Jun 2020 21:13:54: 8000000 INFO @ Sun, 21 Jun 2020 21:13:55: 3000000 INFO @ Sun, 21 Jun 2020 21:13:59: 13000000 INFO @ Sun, 21 Jun 2020 21:14:00: 9000000 INFO @ Sun, 21 Jun 2020 21:14:02: 4000000 INFO @ Sun, 21 Jun 2020 21:14:06: 14000000 INFO @ Sun, 21 Jun 2020 21:14:06: 10000000 INFO @ Sun, 21 Jun 2020 21:14:09: 5000000 INFO @ Sun, 21 Jun 2020 21:14:12: 11000000 INFO @ Sun, 21 Jun 2020 21:14:12: 15000000 INFO @ Sun, 21 Jun 2020 21:14:16: 6000000 INFO @ Sun, 21 Jun 2020 21:14:18: 12000000 INFO @ Sun, 21 Jun 2020 21:14:19: 16000000 INFO @ Sun, 21 Jun 2020 21:14:22: 7000000 INFO @ Sun, 21 Jun 2020 21:14:25: 13000000 INFO @ Sun, 21 Jun 2020 21:14:26: 17000000 INFO @ Sun, 21 Jun 2020 21:14:29: 8000000 INFO @ Sun, 21 Jun 2020 21:14:31: 14000000 INFO @ Sun, 21 Jun 2020 21:14:33: 18000000 INFO @ Sun, 21 Jun 2020 21:14:36: 9000000 INFO @ Sun, 21 Jun 2020 21:14:37: 15000000 INFO @ Sun, 21 Jun 2020 21:14:40: 19000000 INFO @ Sun, 21 Jun 2020 21:14:43: 10000000 INFO @ Sun, 21 Jun 2020 21:14:43: 16000000 INFO @ Sun, 21 Jun 2020 21:14:46: 20000000 INFO @ Sun, 21 Jun 2020 21:14:50: 11000000 INFO @ Sun, 21 Jun 2020 21:14:50: 17000000 INFO @ Sun, 21 Jun 2020 21:14:53: 21000000 INFO @ Sun, 21 Jun 2020 21:14:56: 18000000 INFO @ Sun, 21 Jun 2020 21:14:56: 12000000 INFO @ Sun, 21 Jun 2020 21:14:57: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:14:57: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:14:57: #1 total tags in treatment: 21502082 INFO @ Sun, 21 Jun 2020 21:14:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:14:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:14:58: #1 tags after filtering in treatment: 21502072 INFO @ Sun, 21 Jun 2020 21:14:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:14:58: #1 finished! INFO @ Sun, 21 Jun 2020 21:14:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:14:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:14:59: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 21:14:59: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:14:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:15:03: 19000000 INFO @ Sun, 21 Jun 2020 21:15:03: 13000000 INFO @ Sun, 21 Jun 2020 21:15:09: 20000000 INFO @ Sun, 21 Jun 2020 21:15:10: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:15:15: 21000000 INFO @ Sun, 21 Jun 2020 21:15:17: 15000000 INFO @ Sun, 21 Jun 2020 21:15:19: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:15:19: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:15:19: #1 total tags in treatment: 21502082 INFO @ Sun, 21 Jun 2020 21:15:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:15:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:15:19: #1 tags after filtering in treatment: 21502072 INFO @ Sun, 21 Jun 2020 21:15:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:15:19: #1 finished! INFO @ Sun, 21 Jun 2020 21:15:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:15:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:15:21: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 21:15:21: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:15:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:15:23: 16000000 INFO @ Sun, 21 Jun 2020 21:15:29: 17000000 INFO @ Sun, 21 Jun 2020 21:15:36: 18000000 INFO @ Sun, 21 Jun 2020 21:15:42: 19000000 INFO @ Sun, 21 Jun 2020 21:15:48: 20000000 INFO @ Sun, 21 Jun 2020 21:15:54: 21000000 INFO @ Sun, 21 Jun 2020 21:15:57: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:15:57: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:15:57: #1 total tags in treatment: 21502082 INFO @ Sun, 21 Jun 2020 21:15:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:15:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:15:58: #1 tags after filtering in treatment: 21502072 INFO @ Sun, 21 Jun 2020 21:15:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:15:58: #1 finished! INFO @ Sun, 21 Jun 2020 21:15:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:15:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:15:59: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 21:15:59: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:15:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467059/SRX467059.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。