Job ID = 6457656 SRX = SRX467058 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:46:48 prefetch.2.10.7: 1) Downloading 'SRR1164482'... 2020-06-21T11:46:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:50:10 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:50:10 prefetch.2.10.7: 1) 'SRR1164482' was downloaded successfully Read 29206592 spots for SRR1164482/SRR1164482.sra Written 29206592 spots for SRR1164482/SRR1164482.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:47 29206592 reads; of these: 29206592 (100.00%) were unpaired; of these: 1045765 (3.58%) aligned 0 times 22388070 (76.65%) aligned exactly 1 time 5772757 (19.77%) aligned >1 times 96.42% overall alignment rate Time searching: 00:07:48 Overall time: 00:07:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6658096 / 28160827 = 0.2364 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:05:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:05:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:05:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:05:45: 1000000 INFO @ Sun, 21 Jun 2020 21:05:52: 2000000 INFO @ Sun, 21 Jun 2020 21:05:58: 3000000 INFO @ Sun, 21 Jun 2020 21:06:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:06:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:06:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:06:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:06:12: 5000000 INFO @ Sun, 21 Jun 2020 21:06:16: 1000000 INFO @ Sun, 21 Jun 2020 21:06:19: 6000000 INFO @ Sun, 21 Jun 2020 21:06:24: 2000000 INFO @ Sun, 21 Jun 2020 21:06:26: 7000000 INFO @ Sun, 21 Jun 2020 21:06:31: 3000000 INFO @ Sun, 21 Jun 2020 21:06:33: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:06:38: 4000000 INFO @ Sun, 21 Jun 2020 21:06:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:06:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:06:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:06:41: 9000000 INFO @ Sun, 21 Jun 2020 21:06:45: 1000000 INFO @ Sun, 21 Jun 2020 21:06:46: 5000000 INFO @ Sun, 21 Jun 2020 21:06:48: 10000000 INFO @ Sun, 21 Jun 2020 21:06:51: 2000000 INFO @ Sun, 21 Jun 2020 21:06:53: 6000000 INFO @ Sun, 21 Jun 2020 21:06:56: 11000000 INFO @ Sun, 21 Jun 2020 21:06:58: 3000000 INFO @ Sun, 21 Jun 2020 21:07:00: 7000000 INFO @ Sun, 21 Jun 2020 21:07:03: 12000000 INFO @ Sun, 21 Jun 2020 21:07:04: 4000000 INFO @ Sun, 21 Jun 2020 21:07:08: 8000000 INFO @ Sun, 21 Jun 2020 21:07:11: 13000000 INFO @ Sun, 21 Jun 2020 21:07:11: 5000000 INFO @ Sun, 21 Jun 2020 21:07:15: 9000000 INFO @ Sun, 21 Jun 2020 21:07:18: 6000000 INFO @ Sun, 21 Jun 2020 21:07:18: 14000000 INFO @ Sun, 21 Jun 2020 21:07:23: 10000000 INFO @ Sun, 21 Jun 2020 21:07:24: 7000000 INFO @ Sun, 21 Jun 2020 21:07:26: 15000000 INFO @ Sun, 21 Jun 2020 21:07:30: 11000000 INFO @ Sun, 21 Jun 2020 21:07:31: 8000000 INFO @ Sun, 21 Jun 2020 21:07:33: 16000000 INFO @ Sun, 21 Jun 2020 21:07:37: 9000000 INFO @ Sun, 21 Jun 2020 21:07:38: 12000000 INFO @ Sun, 21 Jun 2020 21:07:40: 17000000 INFO @ Sun, 21 Jun 2020 21:07:43: 10000000 INFO @ Sun, 21 Jun 2020 21:07:45: 13000000 INFO @ Sun, 21 Jun 2020 21:07:48: 18000000 INFO @ Sun, 21 Jun 2020 21:07:50: 11000000 INFO @ Sun, 21 Jun 2020 21:07:52: 14000000 INFO @ Sun, 21 Jun 2020 21:07:55: 19000000 INFO @ Sun, 21 Jun 2020 21:07:56: 12000000 INFO @ Sun, 21 Jun 2020 21:08:00: 15000000 INFO @ Sun, 21 Jun 2020 21:08:03: 20000000 INFO @ Sun, 21 Jun 2020 21:08:03: 13000000 INFO @ Sun, 21 Jun 2020 21:08:07: 16000000 INFO @ Sun, 21 Jun 2020 21:08:09: 14000000 INFO @ Sun, 21 Jun 2020 21:08:10: 21000000 INFO @ Sun, 21 Jun 2020 21:08:14: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:08:14: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:08:14: #1 total tags in treatment: 21502731 INFO @ Sun, 21 Jun 2020 21:08:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:08:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:08:14: 17000000 INFO @ Sun, 21 Jun 2020 21:08:15: #1 tags after filtering in treatment: 21502723 INFO @ Sun, 21 Jun 2020 21:08:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:08:15: #1 finished! INFO @ Sun, 21 Jun 2020 21:08:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:08:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:08:16: 15000000 INFO @ Sun, 21 Jun 2020 21:08:16: #2 number of paired peaks: 56 WARNING @ Sun, 21 Jun 2020 21:08:16: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:08:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:08:22: 18000000 INFO @ Sun, 21 Jun 2020 21:08:22: 16000000 INFO @ Sun, 21 Jun 2020 21:08:28: 17000000 INFO @ Sun, 21 Jun 2020 21:08:29: 19000000 INFO @ Sun, 21 Jun 2020 21:08:35: 18000000 INFO @ Sun, 21 Jun 2020 21:08:36: 20000000 INFO @ Sun, 21 Jun 2020 21:08:41: 19000000 INFO @ Sun, 21 Jun 2020 21:08:43: 21000000 INFO @ Sun, 21 Jun 2020 21:08:47: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:08:47: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:08:47: #1 total tags in treatment: 21502731 INFO @ Sun, 21 Jun 2020 21:08:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:08:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:08:47: 20000000 INFO @ Sun, 21 Jun 2020 21:08:48: #1 tags after filtering in treatment: 21502723 INFO @ Sun, 21 Jun 2020 21:08:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:08:48: #1 finished! INFO @ Sun, 21 Jun 2020 21:08:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:08:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:08:49: #2 number of paired peaks: 56 WARNING @ Sun, 21 Jun 2020 21:08:49: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:08:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:08:53: 21000000 INFO @ Sun, 21 Jun 2020 21:08:56: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:08:56: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:08:56: #1 total tags in treatment: 21502731 INFO @ Sun, 21 Jun 2020 21:08:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:08:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:08:57: #1 tags after filtering in treatment: 21502723 INFO @ Sun, 21 Jun 2020 21:08:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:08:57: #1 finished! INFO @ Sun, 21 Jun 2020 21:08:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:08:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:08:58: #2 number of paired peaks: 56 WARNING @ Sun, 21 Jun 2020 21:08:58: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:08:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467058/SRX467058.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。