Job ID = 6457645 SRX = SRX467053 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:45:33 prefetch.2.10.7: 1) Downloading 'SRR1164477'... 2020-06-21T11:45:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:51:56 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:51:56 prefetch.2.10.7: 1) 'SRR1164477' was downloaded successfully Read 37021579 spots for SRR1164477/SRR1164477.sra Written 37021579 spots for SRR1164477/SRR1164477.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:19 37021579 reads; of these: 37021579 (100.00%) were unpaired; of these: 1808788 (4.89%) aligned 0 times 26734374 (72.21%) aligned exactly 1 time 8478417 (22.90%) aligned >1 times 95.11% overall alignment rate Time searching: 00:10:19 Overall time: 00:10:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10351575 / 35212791 = 0.2940 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:13:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:13:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:13:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:13:39: 1000000 INFO @ Sun, 21 Jun 2020 21:13:45: 2000000 INFO @ Sun, 21 Jun 2020 21:13:50: 3000000 INFO @ Sun, 21 Jun 2020 21:13:56: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:14:02: 5000000 INFO @ Sun, 21 Jun 2020 21:14:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:14:03: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:14:03: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:14:08: 6000000 INFO @ Sun, 21 Jun 2020 21:14:10: 1000000 INFO @ Sun, 21 Jun 2020 21:14:15: 7000000 INFO @ Sun, 21 Jun 2020 21:14:16: 2000000 INFO @ Sun, 21 Jun 2020 21:14:21: 8000000 INFO @ Sun, 21 Jun 2020 21:14:23: 3000000 INFO @ Sun, 21 Jun 2020 21:14:28: 9000000 INFO @ Sun, 21 Jun 2020 21:14:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:14:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:14:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:14:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:14:34: 10000000 INFO @ Sun, 21 Jun 2020 21:14:36: 5000000 INFO @ Sun, 21 Jun 2020 21:14:40: 1000000 INFO @ Sun, 21 Jun 2020 21:14:41: 11000000 INFO @ Sun, 21 Jun 2020 21:14:43: 6000000 INFO @ Sun, 21 Jun 2020 21:14:47: 2000000 INFO @ Sun, 21 Jun 2020 21:14:48: 12000000 INFO @ Sun, 21 Jun 2020 21:14:50: 7000000 INFO @ Sun, 21 Jun 2020 21:14:54: 3000000 INFO @ Sun, 21 Jun 2020 21:14:55: 13000000 INFO @ Sun, 21 Jun 2020 21:14:57: 8000000 INFO @ Sun, 21 Jun 2020 21:15:01: 4000000 INFO @ Sun, 21 Jun 2020 21:15:02: 14000000 INFO @ Sun, 21 Jun 2020 21:15:04: 9000000 INFO @ Sun, 21 Jun 2020 21:15:09: 15000000 INFO @ Sun, 21 Jun 2020 21:15:09: 5000000 INFO @ Sun, 21 Jun 2020 21:15:11: 10000000 INFO @ Sun, 21 Jun 2020 21:15:16: 16000000 INFO @ Sun, 21 Jun 2020 21:15:16: 6000000 INFO @ Sun, 21 Jun 2020 21:15:19: 11000000 INFO @ Sun, 21 Jun 2020 21:15:22: 17000000 INFO @ Sun, 21 Jun 2020 21:15:23: 7000000 INFO @ Sun, 21 Jun 2020 21:15:25: 12000000 INFO @ Sun, 21 Jun 2020 21:15:29: 18000000 INFO @ Sun, 21 Jun 2020 21:15:30: 8000000 INFO @ Sun, 21 Jun 2020 21:15:32: 13000000 INFO @ Sun, 21 Jun 2020 21:15:36: 19000000 INFO @ Sun, 21 Jun 2020 21:15:38: 9000000 INFO @ Sun, 21 Jun 2020 21:15:39: 14000000 INFO @ Sun, 21 Jun 2020 21:15:43: 20000000 INFO @ Sun, 21 Jun 2020 21:15:45: 10000000 INFO @ Sun, 21 Jun 2020 21:15:46: 15000000 INFO @ Sun, 21 Jun 2020 21:15:51: 21000000 INFO @ Sun, 21 Jun 2020 21:15:52: 11000000 INFO @ Sun, 21 Jun 2020 21:15:52: 16000000 INFO @ Sun, 21 Jun 2020 21:15:57: 22000000 INFO @ Sun, 21 Jun 2020 21:15:59: 12000000 INFO @ Sun, 21 Jun 2020 21:15:59: 17000000 INFO @ Sun, 21 Jun 2020 21:16:04: 23000000 INFO @ Sun, 21 Jun 2020 21:16:06: 13000000 INFO @ Sun, 21 Jun 2020 21:16:06: 18000000 INFO @ Sun, 21 Jun 2020 21:16:11: 24000000 INFO @ Sun, 21 Jun 2020 21:16:12: 14000000 INFO @ Sun, 21 Jun 2020 21:16:13: 19000000 INFO @ Sun, 21 Jun 2020 21:16:18: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:16:18: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:16:18: #1 total tags in treatment: 24861216 INFO @ Sun, 21 Jun 2020 21:16:18: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:16:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:16:19: #1 tags after filtering in treatment: 24861215 INFO @ Sun, 21 Jun 2020 21:16:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:16:19: #1 finished! INFO @ Sun, 21 Jun 2020 21:16:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:16:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:16:19: 15000000 INFO @ Sun, 21 Jun 2020 21:16:20: 20000000 INFO @ Sun, 21 Jun 2020 21:16:21: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 21:16:21: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:16:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:16:26: 16000000 INFO @ Sun, 21 Jun 2020 21:16:27: 21000000 INFO @ Sun, 21 Jun 2020 21:16:32: 17000000 INFO @ Sun, 21 Jun 2020 21:16:33: 22000000 INFO @ Sun, 21 Jun 2020 21:16:38: 18000000 INFO @ Sun, 21 Jun 2020 21:16:40: 23000000 INFO @ Sun, 21 Jun 2020 21:16:44: 19000000 INFO @ Sun, 21 Jun 2020 21:16:46: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:16:51: 20000000 INFO @ Sun, 21 Jun 2020 21:16:52: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:16:52: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:16:52: #1 total tags in treatment: 24861216 INFO @ Sun, 21 Jun 2020 21:16:52: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:16:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:16:53: #1 tags after filtering in treatment: 24861215 INFO @ Sun, 21 Jun 2020 21:16:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:16:53: #1 finished! INFO @ Sun, 21 Jun 2020 21:16:53: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:16:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:16:54: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 21:16:54: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:16:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:16:57: 21000000 INFO @ Sun, 21 Jun 2020 21:17:03: 22000000 INFO @ Sun, 21 Jun 2020 21:17:09: 23000000 INFO @ Sun, 21 Jun 2020 21:17:15: 24000000 INFO @ Sun, 21 Jun 2020 21:17:20: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:17:20: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:17:20: #1 total tags in treatment: 24861216 INFO @ Sun, 21 Jun 2020 21:17:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:17:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:17:22: #1 tags after filtering in treatment: 24861215 INFO @ Sun, 21 Jun 2020 21:17:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:17:22: #1 finished! INFO @ Sun, 21 Jun 2020 21:17:22: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:17:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:17:23: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 21:17:23: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:17:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467053/SRX467053.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。