Job ID = 6457641 SRX = SRX467049 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:05:36 prefetch.2.10.7: 1) Downloading 'SRR1164473'... 2020-06-21T12:05:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:15:49 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:15:49 prefetch.2.10.7: 1) 'SRR1164473' was downloaded successfully Read 84993195 spots for SRR1164473/SRR1164473.sra Written 84993195 spots for SRR1164473/SRR1164473.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:58 84993195 reads; of these: 84993195 (100.00%) were unpaired; of these: 3426698 (4.03%) aligned 0 times 63019641 (74.15%) aligned exactly 1 time 18546856 (21.82%) aligned >1 times 95.97% overall alignment rate Time searching: 00:24:59 Overall time: 00:24:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 36540447 / 81566497 = 0.4480 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:00:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:00:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:00:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:01:03: 1000000 INFO @ Sun, 21 Jun 2020 22:01:09: 2000000 INFO @ Sun, 21 Jun 2020 22:01:15: 3000000 INFO @ Sun, 21 Jun 2020 22:01:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:01:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:01:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:01:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:01:28: 5000000 INFO @ Sun, 21 Jun 2020 22:01:33: 1000000 INFO @ Sun, 21 Jun 2020 22:01:34: 6000000 INFO @ Sun, 21 Jun 2020 22:01:40: 2000000 INFO @ Sun, 21 Jun 2020 22:01:40: 7000000 INFO @ Sun, 21 Jun 2020 22:01:46: 3000000 INFO @ Sun, 21 Jun 2020 22:01:46: 8000000 INFO @ Sun, 21 Jun 2020 22:01:52: 4000000 INFO @ Sun, 21 Jun 2020 22:01:52: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:01:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:01:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:01:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:01:58: 10000000 INFO @ Sun, 21 Jun 2020 22:01:58: 5000000 INFO @ Sun, 21 Jun 2020 22:02:03: 1000000 INFO @ Sun, 21 Jun 2020 22:02:05: 11000000 INFO @ Sun, 21 Jun 2020 22:02:05: 6000000 INFO @ Sun, 21 Jun 2020 22:02:10: 2000000 INFO @ Sun, 21 Jun 2020 22:02:11: 12000000 INFO @ Sun, 21 Jun 2020 22:02:11: 7000000 INFO @ Sun, 21 Jun 2020 22:02:16: 3000000 INFO @ Sun, 21 Jun 2020 22:02:17: 13000000 INFO @ Sun, 21 Jun 2020 22:02:17: 8000000 INFO @ Sun, 21 Jun 2020 22:02:22: 4000000 INFO @ Sun, 21 Jun 2020 22:02:23: 14000000 INFO @ Sun, 21 Jun 2020 22:02:23: 9000000 INFO @ Sun, 21 Jun 2020 22:02:28: 5000000 INFO @ Sun, 21 Jun 2020 22:02:29: 10000000 INFO @ Sun, 21 Jun 2020 22:02:29: 15000000 INFO @ Sun, 21 Jun 2020 22:02:34: 6000000 INFO @ Sun, 21 Jun 2020 22:02:35: 11000000 INFO @ Sun, 21 Jun 2020 22:02:36: 16000000 INFO @ Sun, 21 Jun 2020 22:02:40: 7000000 INFO @ Sun, 21 Jun 2020 22:02:41: 12000000 INFO @ Sun, 21 Jun 2020 22:02:42: 17000000 INFO @ Sun, 21 Jun 2020 22:02:46: 8000000 INFO @ Sun, 21 Jun 2020 22:02:48: 13000000 INFO @ Sun, 21 Jun 2020 22:02:48: 18000000 INFO @ Sun, 21 Jun 2020 22:02:52: 9000000 INFO @ Sun, 21 Jun 2020 22:02:54: 14000000 INFO @ Sun, 21 Jun 2020 22:02:54: 19000000 INFO @ Sun, 21 Jun 2020 22:02:58: 10000000 INFO @ Sun, 21 Jun 2020 22:03:00: 15000000 INFO @ Sun, 21 Jun 2020 22:03:01: 20000000 INFO @ Sun, 21 Jun 2020 22:03:04: 11000000 INFO @ Sun, 21 Jun 2020 22:03:06: 16000000 INFO @ Sun, 21 Jun 2020 22:03:07: 21000000 INFO @ Sun, 21 Jun 2020 22:03:10: 12000000 INFO @ Sun, 21 Jun 2020 22:03:12: 17000000 INFO @ Sun, 21 Jun 2020 22:03:13: 22000000 INFO @ Sun, 21 Jun 2020 22:03:16: 13000000 INFO @ Sun, 21 Jun 2020 22:03:19: 18000000 INFO @ Sun, 21 Jun 2020 22:03:19: 23000000 INFO @ Sun, 21 Jun 2020 22:03:22: 14000000 INFO @ Sun, 21 Jun 2020 22:03:25: 24000000 INFO @ Sun, 21 Jun 2020 22:03:25: 19000000 INFO @ Sun, 21 Jun 2020 22:03:29: 15000000 INFO @ Sun, 21 Jun 2020 22:03:31: 25000000 INFO @ Sun, 21 Jun 2020 22:03:31: 20000000 INFO @ Sun, 21 Jun 2020 22:03:35: 16000000 INFO @ Sun, 21 Jun 2020 22:03:37: 21000000 INFO @ Sun, 21 Jun 2020 22:03:37: 26000000 INFO @ Sun, 21 Jun 2020 22:03:41: 17000000 INFO @ Sun, 21 Jun 2020 22:03:43: 22000000 INFO @ Sun, 21 Jun 2020 22:03:43: 27000000 INFO @ Sun, 21 Jun 2020 22:03:47: 18000000 INFO @ Sun, 21 Jun 2020 22:03:49: 23000000 INFO @ Sun, 21 Jun 2020 22:03:49: 28000000 INFO @ Sun, 21 Jun 2020 22:03:53: 19000000 INFO @ Sun, 21 Jun 2020 22:03:55: 29000000 INFO @ Sun, 21 Jun 2020 22:03:55: 24000000 INFO @ Sun, 21 Jun 2020 22:03:59: 20000000 INFO @ Sun, 21 Jun 2020 22:04:01: 30000000 INFO @ Sun, 21 Jun 2020 22:04:01: 25000000 INFO @ Sun, 21 Jun 2020 22:04:05: 21000000 INFO @ Sun, 21 Jun 2020 22:04:07: 31000000 INFO @ Sun, 21 Jun 2020 22:04:07: 26000000 INFO @ Sun, 21 Jun 2020 22:04:11: 22000000 INFO @ Sun, 21 Jun 2020 22:04:13: 32000000 INFO @ Sun, 21 Jun 2020 22:04:14: 27000000 INFO @ Sun, 21 Jun 2020 22:04:17: 23000000 INFO @ Sun, 21 Jun 2020 22:04:18: 33000000 INFO @ Sun, 21 Jun 2020 22:04:20: 28000000 INFO @ Sun, 21 Jun 2020 22:04:23: 24000000 INFO @ Sun, 21 Jun 2020 22:04:24: 34000000 INFO @ Sun, 21 Jun 2020 22:04:26: 29000000 INFO @ Sun, 21 Jun 2020 22:04:29: 25000000 INFO @ Sun, 21 Jun 2020 22:04:30: 35000000 INFO @ Sun, 21 Jun 2020 22:04:32: 30000000 INFO @ Sun, 21 Jun 2020 22:04:35: 26000000 INFO @ Sun, 21 Jun 2020 22:04:36: 36000000 INFO @ Sun, 21 Jun 2020 22:04:37: 31000000 INFO @ Sun, 21 Jun 2020 22:04:41: 27000000 INFO @ Sun, 21 Jun 2020 22:04:42: 37000000 INFO @ Sun, 21 Jun 2020 22:04:43: 32000000 INFO @ Sun, 21 Jun 2020 22:04:47: 28000000 INFO @ Sun, 21 Jun 2020 22:04:48: 38000000 INFO @ Sun, 21 Jun 2020 22:04:49: 33000000 INFO @ Sun, 21 Jun 2020 22:04:52: 29000000 INFO @ Sun, 21 Jun 2020 22:04:54: 39000000 INFO @ Sun, 21 Jun 2020 22:04:55: 34000000 INFO @ Sun, 21 Jun 2020 22:04:58: 30000000 INFO @ Sun, 21 Jun 2020 22:05:00: 40000000 INFO @ Sun, 21 Jun 2020 22:05:01: 35000000 INFO @ Sun, 21 Jun 2020 22:05:04: 31000000 INFO @ Sun, 21 Jun 2020 22:05:06: 41000000 INFO @ Sun, 21 Jun 2020 22:05:07: 36000000 INFO @ Sun, 21 Jun 2020 22:05:10: 32000000 INFO @ Sun, 21 Jun 2020 22:05:12: 42000000 INFO @ Sun, 21 Jun 2020 22:05:13: 37000000 INFO @ Sun, 21 Jun 2020 22:05:15: 33000000 INFO @ Sun, 21 Jun 2020 22:05:18: 43000000 INFO @ Sun, 21 Jun 2020 22:05:19: 38000000 INFO @ Sun, 21 Jun 2020 22:05:21: 34000000 INFO @ Sun, 21 Jun 2020 22:05:23: 44000000 INFO @ Sun, 21 Jun 2020 22:05:25: 39000000 INFO @ Sun, 21 Jun 2020 22:05:27: 35000000 INFO @ Sun, 21 Jun 2020 22:05:30: 45000000 INFO @ Sun, 21 Jun 2020 22:05:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:05:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:05:30: #1 total tags in treatment: 45026050 INFO @ Sun, 21 Jun 2020 22:05:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:05:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:05:31: #1 tags after filtering in treatment: 45026050 INFO @ Sun, 21 Jun 2020 22:05:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:05:31: #1 finished! INFO @ Sun, 21 Jun 2020 22:05:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:05:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:05:31: 40000000 INFO @ Sun, 21 Jun 2020 22:05:33: 36000000 INFO @ Sun, 21 Jun 2020 22:05:34: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 22:05:34: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:05:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:05:37: 41000000 INFO @ Sun, 21 Jun 2020 22:05:40: 37000000 INFO @ Sun, 21 Jun 2020 22:05:43: 42000000 INFO @ Sun, 21 Jun 2020 22:05:46: 38000000 INFO @ Sun, 21 Jun 2020 22:05:48: 43000000 INFO @ Sun, 21 Jun 2020 22:05:52: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:05:54: 44000000 INFO @ Sun, 21 Jun 2020 22:05:59: 40000000 INFO @ Sun, 21 Jun 2020 22:06:00: 45000000 INFO @ Sun, 21 Jun 2020 22:06:01: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:06:01: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:06:01: #1 total tags in treatment: 45026050 INFO @ Sun, 21 Jun 2020 22:06:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:06:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:06:02: #1 tags after filtering in treatment: 45026050 INFO @ Sun, 21 Jun 2020 22:06:02: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:06:02: #1 finished! INFO @ Sun, 21 Jun 2020 22:06:02: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:06:02: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:06:05: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 22:06:05: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:06:05: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 22:06:05: 41000000 cut: /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:06:11: 42000000 INFO @ Sun, 21 Jun 2020 22:06:17: 43000000 INFO @ Sun, 21 Jun 2020 22:06:23: 44000000 INFO @ Sun, 21 Jun 2020 22:06:29: 45000000 INFO @ Sun, 21 Jun 2020 22:06:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:06:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:06:30: #1 total tags in treatment: 45026050 INFO @ Sun, 21 Jun 2020 22:06:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:06:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:06:31: #1 tags after filtering in treatment: 45026050 INFO @ Sun, 21 Jun 2020 22:06:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:06:31: #1 finished! INFO @ Sun, 21 Jun 2020 22:06:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:06:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:06:33: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 22:06:33: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:06:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467049/SRX467049.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。