Job ID = 6457634 SRX = SRX467045 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:59:22 prefetch.2.10.7: 1) Downloading 'SRR1164469'... 2020-06-21T11:59:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:07:47 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:07:47 prefetch.2.10.7: 1) 'SRR1164469' was downloaded successfully Read 48787082 spots for SRR1164469/SRR1164469.sra Written 48787082 spots for SRR1164469/SRR1164469.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:02 48787082 reads; of these: 48787082 (100.00%) were unpaired; of these: 2735927 (5.61%) aligned 0 times 38141795 (78.18%) aligned exactly 1 time 7909360 (16.21%) aligned >1 times 94.39% overall alignment rate Time searching: 00:13:02 Overall time: 00:13:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 14088293 / 46051155 = 0.3059 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:32:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:32:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:32:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:32:36: 1000000 INFO @ Sun, 21 Jun 2020 21:32:42: 2000000 INFO @ Sun, 21 Jun 2020 21:32:47: 3000000 INFO @ Sun, 21 Jun 2020 21:32:53: 4000000 INFO @ Sun, 21 Jun 2020 21:32:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:33:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:33:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:33:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:33:04: 6000000 INFO @ Sun, 21 Jun 2020 21:33:07: 1000000 INFO @ Sun, 21 Jun 2020 21:33:11: 7000000 INFO @ Sun, 21 Jun 2020 21:33:14: 2000000 INFO @ Sun, 21 Jun 2020 21:33:18: 8000000 INFO @ Sun, 21 Jun 2020 21:33:20: 3000000 INFO @ Sun, 21 Jun 2020 21:33:24: 9000000 INFO @ Sun, 21 Jun 2020 21:33:27: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:33:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:33:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:33:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:33:31: 10000000 INFO @ Sun, 21 Jun 2020 21:33:34: 5000000 INFO @ Sun, 21 Jun 2020 21:33:38: 11000000 INFO @ Sun, 21 Jun 2020 21:33:38: 1000000 INFO @ Sun, 21 Jun 2020 21:33:41: 6000000 INFO @ Sun, 21 Jun 2020 21:33:45: 12000000 INFO @ Sun, 21 Jun 2020 21:33:46: 2000000 INFO @ Sun, 21 Jun 2020 21:33:49: 7000000 INFO @ Sun, 21 Jun 2020 21:33:53: 13000000 INFO @ Sun, 21 Jun 2020 21:33:54: 3000000 INFO @ Sun, 21 Jun 2020 21:33:56: 8000000 INFO @ Sun, 21 Jun 2020 21:34:00: 14000000 INFO @ Sun, 21 Jun 2020 21:34:02: 4000000 INFO @ Sun, 21 Jun 2020 21:34:04: 9000000 INFO @ Sun, 21 Jun 2020 21:34:08: 15000000 INFO @ Sun, 21 Jun 2020 21:34:10: 5000000 INFO @ Sun, 21 Jun 2020 21:34:11: 10000000 INFO @ Sun, 21 Jun 2020 21:34:15: 16000000 INFO @ Sun, 21 Jun 2020 21:34:18: 6000000 INFO @ Sun, 21 Jun 2020 21:34:19: 11000000 INFO @ Sun, 21 Jun 2020 21:34:23: 17000000 INFO @ Sun, 21 Jun 2020 21:34:26: 12000000 INFO @ Sun, 21 Jun 2020 21:34:26: 7000000 INFO @ Sun, 21 Jun 2020 21:34:30: 18000000 INFO @ Sun, 21 Jun 2020 21:34:34: 13000000 INFO @ Sun, 21 Jun 2020 21:34:35: 8000000 INFO @ Sun, 21 Jun 2020 21:34:38: 19000000 INFO @ Sun, 21 Jun 2020 21:34:41: 14000000 INFO @ Sun, 21 Jun 2020 21:34:43: 9000000 INFO @ Sun, 21 Jun 2020 21:34:46: 20000000 INFO @ Sun, 21 Jun 2020 21:34:49: 15000000 INFO @ Sun, 21 Jun 2020 21:34:51: 10000000 INFO @ Sun, 21 Jun 2020 21:34:53: 21000000 INFO @ Sun, 21 Jun 2020 21:34:56: 16000000 INFO @ Sun, 21 Jun 2020 21:34:59: 11000000 INFO @ Sun, 21 Jun 2020 21:35:01: 22000000 INFO @ Sun, 21 Jun 2020 21:35:04: 17000000 INFO @ Sun, 21 Jun 2020 21:35:07: 12000000 INFO @ Sun, 21 Jun 2020 21:35:08: 23000000 INFO @ Sun, 21 Jun 2020 21:35:12: 18000000 INFO @ Sun, 21 Jun 2020 21:35:15: 13000000 INFO @ Sun, 21 Jun 2020 21:35:16: 24000000 INFO @ Sun, 21 Jun 2020 21:35:19: 19000000 INFO @ Sun, 21 Jun 2020 21:35:23: 14000000 INFO @ Sun, 21 Jun 2020 21:35:24: 25000000 INFO @ Sun, 21 Jun 2020 21:35:27: 20000000 INFO @ Sun, 21 Jun 2020 21:35:31: 15000000 INFO @ Sun, 21 Jun 2020 21:35:31: 26000000 INFO @ Sun, 21 Jun 2020 21:35:35: 21000000 INFO @ Sun, 21 Jun 2020 21:35:39: 16000000 INFO @ Sun, 21 Jun 2020 21:35:40: 27000000 INFO @ Sun, 21 Jun 2020 21:35:43: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:35:47: 17000000 INFO @ Sun, 21 Jun 2020 21:35:47: 28000000 INFO @ Sun, 21 Jun 2020 21:35:50: 23000000 INFO @ Sun, 21 Jun 2020 21:35:55: 18000000 INFO @ Sun, 21 Jun 2020 21:35:55: 29000000 INFO @ Sun, 21 Jun 2020 21:35:58: 24000000 INFO @ Sun, 21 Jun 2020 21:36:03: 30000000 INFO @ Sun, 21 Jun 2020 21:36:03: 19000000 INFO @ Sun, 21 Jun 2020 21:36:06: 25000000 INFO @ Sun, 21 Jun 2020 21:36:11: 31000000 INFO @ Sun, 21 Jun 2020 21:36:11: 20000000 INFO @ Sun, 21 Jun 2020 21:36:14: 26000000 INFO @ Sun, 21 Jun 2020 21:36:19: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:36:19: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:36:19: #1 total tags in treatment: 31962862 INFO @ Sun, 21 Jun 2020 21:36:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:36:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:36:19: 21000000 INFO @ Sun, 21 Jun 2020 21:36:19: #1 tags after filtering in treatment: 31962859 INFO @ Sun, 21 Jun 2020 21:36:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:36:19: #1 finished! INFO @ Sun, 21 Jun 2020 21:36:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:36:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:36:21: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:36:21: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:36:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:36:22: 27000000 INFO @ Sun, 21 Jun 2020 21:36:28: 22000000 INFO @ Sun, 21 Jun 2020 21:36:29: 28000000 INFO @ Sun, 21 Jun 2020 21:36:36: 23000000 INFO @ Sun, 21 Jun 2020 21:36:37: 29000000 INFO @ Sun, 21 Jun 2020 21:36:44: 24000000 INFO @ Sun, 21 Jun 2020 21:36:45: 30000000 INFO @ Sun, 21 Jun 2020 21:36:52: 25000000 INFO @ Sun, 21 Jun 2020 21:36:52: 31000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:37:00: 26000000 INFO @ Sun, 21 Jun 2020 21:37:00: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:37:00: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:37:00: #1 total tags in treatment: 31962862 INFO @ Sun, 21 Jun 2020 21:37:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:37:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:37:01: #1 tags after filtering in treatment: 31962859 INFO @ Sun, 21 Jun 2020 21:37:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:37:01: #1 finished! INFO @ Sun, 21 Jun 2020 21:37:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:37:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:37:03: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:37:03: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:37:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:37:08: 27000000 INFO @ Sun, 21 Jun 2020 21:37:15: 28000000 INFO @ Sun, 21 Jun 2020 21:37:22: 29000000 INFO @ Sun, 21 Jun 2020 21:37:29: 30000000 INFO @ Sun, 21 Jun 2020 21:37:36: 31000000 INFO @ Sun, 21 Jun 2020 21:37:44: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:37:44: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:37:44: #1 total tags in treatment: 31962862 INFO @ Sun, 21 Jun 2020 21:37:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:37:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:37:45: #1 tags after filtering in treatment: 31962859 INFO @ Sun, 21 Jun 2020 21:37:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:37:45: #1 finished! INFO @ Sun, 21 Jun 2020 21:37:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:37:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:37:46: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:37:46: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:37:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467045/SRX467045.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling