Job ID = 6457627 SRX = SRX467038 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:50:18 prefetch.2.10.7: 1) Downloading 'SRR1164462'... 2020-06-21T11:50:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:55:45 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:55:45 prefetch.2.10.7: 1) 'SRR1164462' was downloaded successfully Read 38980643 spots for SRR1164462/SRR1164462.sra Written 38980643 spots for SRR1164462/SRR1164462.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:03 38980643 reads; of these: 38980643 (100.00%) were unpaired; of these: 1982816 (5.09%) aligned 0 times 31435615 (80.64%) aligned exactly 1 time 5562212 (14.27%) aligned >1 times 94.91% overall alignment rate Time searching: 00:09:03 Overall time: 00:09:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 14009433 / 36997827 = 0.3787 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:13:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:13:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:13:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:13:59: 1000000 INFO @ Sun, 21 Jun 2020 21:14:04: 2000000 INFO @ Sun, 21 Jun 2020 21:14:08: 3000000 INFO @ Sun, 21 Jun 2020 21:14:13: 4000000 INFO @ Sun, 21 Jun 2020 21:14:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:14:22: 6000000 INFO @ Sun, 21 Jun 2020 21:14:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:14:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:14:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:14:27: 7000000 INFO @ Sun, 21 Jun 2020 21:14:29: 1000000 INFO @ Sun, 21 Jun 2020 21:14:32: 8000000 INFO @ Sun, 21 Jun 2020 21:14:34: 2000000 INFO @ Sun, 21 Jun 2020 21:14:37: 9000000 INFO @ Sun, 21 Jun 2020 21:14:39: 3000000 INFO @ Sun, 21 Jun 2020 21:14:42: 10000000 INFO @ Sun, 21 Jun 2020 21:14:44: 4000000 INFO @ Sun, 21 Jun 2020 21:14:47: 11000000 INFO @ Sun, 21 Jun 2020 21:14:49: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:14:52: 12000000 INFO @ Sun, 21 Jun 2020 21:14:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:14:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:14:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:14:54: 6000000 INFO @ Sun, 21 Jun 2020 21:14:57: 13000000 INFO @ Sun, 21 Jun 2020 21:14:59: 7000000 INFO @ Sun, 21 Jun 2020 21:14:59: 1000000 INFO @ Sun, 21 Jun 2020 21:15:02: 14000000 INFO @ Sun, 21 Jun 2020 21:15:04: 8000000 INFO @ Sun, 21 Jun 2020 21:15:04: 2000000 INFO @ Sun, 21 Jun 2020 21:15:07: 15000000 INFO @ Sun, 21 Jun 2020 21:15:09: 9000000 INFO @ Sun, 21 Jun 2020 21:15:09: 3000000 INFO @ Sun, 21 Jun 2020 21:15:12: 16000000 INFO @ Sun, 21 Jun 2020 21:15:14: 10000000 INFO @ Sun, 21 Jun 2020 21:15:14: 4000000 INFO @ Sun, 21 Jun 2020 21:15:17: 17000000 INFO @ Sun, 21 Jun 2020 21:15:19: 11000000 INFO @ Sun, 21 Jun 2020 21:15:19: 5000000 INFO @ Sun, 21 Jun 2020 21:15:22: 18000000 INFO @ Sun, 21 Jun 2020 21:15:24: 12000000 INFO @ Sun, 21 Jun 2020 21:15:24: 6000000 INFO @ Sun, 21 Jun 2020 21:15:28: 19000000 INFO @ Sun, 21 Jun 2020 21:15:29: 13000000 INFO @ Sun, 21 Jun 2020 21:15:29: 7000000 INFO @ Sun, 21 Jun 2020 21:15:33: 20000000 INFO @ Sun, 21 Jun 2020 21:15:34: 14000000 INFO @ Sun, 21 Jun 2020 21:15:34: 8000000 INFO @ Sun, 21 Jun 2020 21:15:38: 21000000 INFO @ Sun, 21 Jun 2020 21:15:39: 15000000 INFO @ Sun, 21 Jun 2020 21:15:40: 9000000 INFO @ Sun, 21 Jun 2020 21:15:43: 22000000 INFO @ Sun, 21 Jun 2020 21:15:44: 16000000 INFO @ Sun, 21 Jun 2020 21:15:45: 10000000 INFO @ Sun, 21 Jun 2020 21:15:49: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:15:49: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:15:49: #1 total tags in treatment: 22988394 INFO @ Sun, 21 Jun 2020 21:15:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:15:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:15:49: #1 tags after filtering in treatment: 22988393 INFO @ Sun, 21 Jun 2020 21:15:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:15:49: #1 finished! INFO @ Sun, 21 Jun 2020 21:15:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:15:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:15:49: 17000000 INFO @ Sun, 21 Jun 2020 21:15:50: 11000000 INFO @ Sun, 21 Jun 2020 21:15:51: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 21:15:51: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:15:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:15:55: 18000000 INFO @ Sun, 21 Jun 2020 21:15:55: 12000000 INFO @ Sun, 21 Jun 2020 21:16:00: 19000000 INFO @ Sun, 21 Jun 2020 21:16:00: 13000000 INFO @ Sun, 21 Jun 2020 21:16:05: 14000000 INFO @ Sun, 21 Jun 2020 21:16:05: 20000000 INFO @ Sun, 21 Jun 2020 21:16:10: 15000000 INFO @ Sun, 21 Jun 2020 21:16:10: 21000000 INFO @ Sun, 21 Jun 2020 21:16:15: 16000000 INFO @ Sun, 21 Jun 2020 21:16:16: 22000000 INFO @ Sun, 21 Jun 2020 21:16:20: 17000000 INFO @ Sun, 21 Jun 2020 21:16:21: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:16:21: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:16:21: #1 total tags in treatment: 22988394 INFO @ Sun, 21 Jun 2020 21:16:21: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:16:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:16:22: #1 tags after filtering in treatment: 22988393 INFO @ Sun, 21 Jun 2020 21:16:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:16:22: #1 finished! INFO @ Sun, 21 Jun 2020 21:16:22: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:16:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:16:23: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 21:16:23: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:16:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:16:25: 18000000 INFO @ Sun, 21 Jun 2020 21:16:31: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:16:36: 20000000 INFO @ Sun, 21 Jun 2020 21:16:41: 21000000 INFO @ Sun, 21 Jun 2020 21:16:46: 22000000 INFO @ Sun, 21 Jun 2020 21:16:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:16:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:16:51: #1 total tags in treatment: 22988394 INFO @ Sun, 21 Jun 2020 21:16:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:16:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:16:52: #1 tags after filtering in treatment: 22988393 INFO @ Sun, 21 Jun 2020 21:16:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:16:52: #1 finished! INFO @ Sun, 21 Jun 2020 21:16:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:16:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:16:53: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 21:16:53: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:16:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467038/SRX467038.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。