Job ID = 6457622 SRX = SRX467036 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:02:17 prefetch.2.10.7: 1) Downloading 'SRR1164460'... 2020-06-21T12:02:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:05:45 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:05:45 prefetch.2.10.7: 1) 'SRR1164460' was downloaded successfully Read 17765478 spots for SRR1164460/SRR1164460.sra Written 17765478 spots for SRR1164460/SRR1164460.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:41 17765478 reads; of these: 17765478 (100.00%) were unpaired; of these: 578653 (3.26%) aligned 0 times 14803170 (83.33%) aligned exactly 1 time 2383655 (13.42%) aligned >1 times 96.74% overall alignment rate Time searching: 00:04:41 Overall time: 00:04:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2885961 / 17186825 = 0.1679 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:15:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:15:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:15:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:15:54: 1000000 INFO @ Sun, 21 Jun 2020 21:16:00: 2000000 INFO @ Sun, 21 Jun 2020 21:16:05: 3000000 INFO @ Sun, 21 Jun 2020 21:16:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:16:16: 5000000 INFO @ Sun, 21 Jun 2020 21:16:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:16:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:16:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:16:22: 6000000 INFO @ Sun, 21 Jun 2020 21:16:25: 1000000 INFO @ Sun, 21 Jun 2020 21:16:29: 7000000 INFO @ Sun, 21 Jun 2020 21:16:31: 2000000 INFO @ Sun, 21 Jun 2020 21:16:35: 8000000 INFO @ Sun, 21 Jun 2020 21:16:37: 3000000 INFO @ Sun, 21 Jun 2020 21:16:41: 9000000 INFO @ Sun, 21 Jun 2020 21:16:43: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:16:47: 10000000 INFO @ Sun, 21 Jun 2020 21:16:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:16:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:16:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:16:50: 5000000 INFO @ Sun, 21 Jun 2020 21:16:54: 11000000 INFO @ Sun, 21 Jun 2020 21:16:55: 1000000 INFO @ Sun, 21 Jun 2020 21:16:56: 6000000 INFO @ Sun, 21 Jun 2020 21:17:00: 12000000 INFO @ Sun, 21 Jun 2020 21:17:01: 2000000 INFO @ Sun, 21 Jun 2020 21:17:02: 7000000 INFO @ Sun, 21 Jun 2020 21:17:07: 13000000 INFO @ Sun, 21 Jun 2020 21:17:07: 3000000 INFO @ Sun, 21 Jun 2020 21:17:09: 8000000 INFO @ Sun, 21 Jun 2020 21:17:13: 14000000 INFO @ Sun, 21 Jun 2020 21:17:14: 4000000 INFO @ Sun, 21 Jun 2020 21:17:15: 9000000 INFO @ Sun, 21 Jun 2020 21:17:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:17:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:17:15: #1 total tags in treatment: 14300864 INFO @ Sun, 21 Jun 2020 21:17:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:17:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:17:16: #1 tags after filtering in treatment: 14300844 INFO @ Sun, 21 Jun 2020 21:17:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:17:16: #1 finished! INFO @ Sun, 21 Jun 2020 21:17:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:17:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:17:17: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 21:17:17: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:17:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:17:20: 5000000 INFO @ Sun, 21 Jun 2020 21:17:21: 10000000 INFO @ Sun, 21 Jun 2020 21:17:26: 6000000 INFO @ Sun, 21 Jun 2020 21:17:27: 11000000 INFO @ Sun, 21 Jun 2020 21:17:32: 7000000 INFO @ Sun, 21 Jun 2020 21:17:34: 12000000 INFO @ Sun, 21 Jun 2020 21:17:38: 8000000 INFO @ Sun, 21 Jun 2020 21:17:40: 13000000 INFO @ Sun, 21 Jun 2020 21:17:45: 9000000 INFO @ Sun, 21 Jun 2020 21:17:46: 14000000 INFO @ Sun, 21 Jun 2020 21:17:48: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:17:48: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:17:48: #1 total tags in treatment: 14300864 INFO @ Sun, 21 Jun 2020 21:17:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:17:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:17:49: #1 tags after filtering in treatment: 14300844 INFO @ Sun, 21 Jun 2020 21:17:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:17:49: #1 finished! INFO @ Sun, 21 Jun 2020 21:17:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:17:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:17:50: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 21:17:50: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:17:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:17:51: 10000000 INFO @ Sun, 21 Jun 2020 21:17:56: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:18:02: 12000000 INFO @ Sun, 21 Jun 2020 21:18:08: 13000000 INFO @ Sun, 21 Jun 2020 21:18:14: 14000000 INFO @ Sun, 21 Jun 2020 21:18:16: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:18:16: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:18:16: #1 total tags in treatment: 14300864 INFO @ Sun, 21 Jun 2020 21:18:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:18:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:18:16: #1 tags after filtering in treatment: 14300844 INFO @ Sun, 21 Jun 2020 21:18:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:18:16: #1 finished! INFO @ Sun, 21 Jun 2020 21:18:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:18:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:18:17: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 21:18:17: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:18:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467036/SRX467036.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。