Job ID = 6457621 SRX = SRX467035 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:10:04 prefetch.2.10.7: 1) Downloading 'SRR1164459'... 2020-06-21T12:10:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:14:08 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:14:08 prefetch.2.10.7: 1) 'SRR1164459' was downloaded successfully Read 17765478 spots for SRR1164459/SRR1164459.sra Written 17765478 spots for SRR1164459/SRR1164459.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:11 17765478 reads; of these: 17765478 (100.00%) were unpaired; of these: 578539 (3.26%) aligned 0 times 14803213 (83.33%) aligned exactly 1 time 2383726 (13.42%) aligned >1 times 96.74% overall alignment rate Time searching: 00:04:11 Overall time: 00:04:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2886305 / 17186939 = 0.1679 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:23:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:23:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:23:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:23:19: 1000000 INFO @ Sun, 21 Jun 2020 21:23:25: 2000000 INFO @ Sun, 21 Jun 2020 21:23:30: 3000000 INFO @ Sun, 21 Jun 2020 21:23:35: 4000000 INFO @ Sun, 21 Jun 2020 21:23:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:23:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:23:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:23:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:23:46: 6000000 INFO @ Sun, 21 Jun 2020 21:23:51: 1000000 INFO @ Sun, 21 Jun 2020 21:23:52: 7000000 INFO @ Sun, 21 Jun 2020 21:23:58: 2000000 INFO @ Sun, 21 Jun 2020 21:23:58: 8000000 INFO @ Sun, 21 Jun 2020 21:24:04: 3000000 INFO @ Sun, 21 Jun 2020 21:24:05: 9000000 INFO @ Sun, 21 Jun 2020 21:24:11: 10000000 INFO @ Sun, 21 Jun 2020 21:24:11: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:24:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:24:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:24:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:24:17: 11000000 INFO @ Sun, 21 Jun 2020 21:24:18: 5000000 INFO @ Sun, 21 Jun 2020 21:24:20: 1000000 INFO @ Sun, 21 Jun 2020 21:24:23: 12000000 INFO @ Sun, 21 Jun 2020 21:24:25: 6000000 INFO @ Sun, 21 Jun 2020 21:24:26: 2000000 INFO @ Sun, 21 Jun 2020 21:24:29: 13000000 INFO @ Sun, 21 Jun 2020 21:24:32: 3000000 INFO @ Sun, 21 Jun 2020 21:24:32: 7000000 INFO @ Sun, 21 Jun 2020 21:24:35: 14000000 INFO @ Sun, 21 Jun 2020 21:24:38: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:24:38: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:24:38: #1 total tags in treatment: 14300634 INFO @ Sun, 21 Jun 2020 21:24:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:24:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:24:38: 4000000 INFO @ Sun, 21 Jun 2020 21:24:38: #1 tags after filtering in treatment: 14300615 INFO @ Sun, 21 Jun 2020 21:24:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:24:38: #1 finished! INFO @ Sun, 21 Jun 2020 21:24:38: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:24:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:24:39: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 21:24:39: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:24:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:24:39: 8000000 INFO @ Sun, 21 Jun 2020 21:24:44: 5000000 INFO @ Sun, 21 Jun 2020 21:24:46: 9000000 INFO @ Sun, 21 Jun 2020 21:24:50: 6000000 INFO @ Sun, 21 Jun 2020 21:24:53: 10000000 INFO @ Sun, 21 Jun 2020 21:24:56: 7000000 INFO @ Sun, 21 Jun 2020 21:25:01: 11000000 INFO @ Sun, 21 Jun 2020 21:25:02: 8000000 INFO @ Sun, 21 Jun 2020 21:25:08: 9000000 INFO @ Sun, 21 Jun 2020 21:25:08: 12000000 INFO @ Sun, 21 Jun 2020 21:25:13: 10000000 INFO @ Sun, 21 Jun 2020 21:25:15: 13000000 INFO @ Sun, 21 Jun 2020 21:25:19: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:25:22: 14000000 INFO @ Sun, 21 Jun 2020 21:25:24: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:25:24: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:25:24: #1 total tags in treatment: 14300634 INFO @ Sun, 21 Jun 2020 21:25:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:25:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:25:25: #1 tags after filtering in treatment: 14300615 INFO @ Sun, 21 Jun 2020 21:25:25: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:25:25: #1 finished! INFO @ Sun, 21 Jun 2020 21:25:25: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:25:25: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:25:26: 12000000 INFO @ Sun, 21 Jun 2020 21:25:26: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 21:25:26: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:25:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:25:31: 13000000 INFO @ Sun, 21 Jun 2020 21:25:36: 14000000 INFO @ Sun, 21 Jun 2020 21:25:38: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:25:38: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:25:38: #1 total tags in treatment: 14300634 INFO @ Sun, 21 Jun 2020 21:25:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:25:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:25:39: #1 tags after filtering in treatment: 14300615 INFO @ Sun, 21 Jun 2020 21:25:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:25:39: #1 finished! INFO @ Sun, 21 Jun 2020 21:25:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:25:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:25:40: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 21:25:40: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:25:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467035/SRX467035.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。