Job ID = 6457620 SRX = SRX467034 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:55:33 prefetch.2.10.7: 1) Downloading 'SRR1164458'... 2020-06-21T11:55:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:03:01 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:03:02 prefetch.2.10.7: 1) 'SRR1164458' was downloaded successfully Read 29175008 spots for SRR1164458/SRR1164458.sra Written 29175008 spots for SRR1164458/SRR1164458.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:18 29175008 reads; of these: 29175008 (100.00%) were unpaired; of these: 2127381 (7.29%) aligned 0 times 24386102 (83.59%) aligned exactly 1 time 2661525 (9.12%) aligned >1 times 92.71% overall alignment rate Time searching: 00:06:18 Overall time: 00:06:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7639402 / 27047627 = 0.2824 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:16:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:16:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:16:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:16:37: 1000000 INFO @ Sun, 21 Jun 2020 21:16:43: 2000000 INFO @ Sun, 21 Jun 2020 21:16:49: 3000000 INFO @ Sun, 21 Jun 2020 21:16:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:17:01: 5000000 INFO @ Sun, 21 Jun 2020 21:17:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:17:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:17:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:17:07: 1000000 INFO @ Sun, 21 Jun 2020 21:17:07: 6000000 INFO @ Sun, 21 Jun 2020 21:17:13: 2000000 INFO @ Sun, 21 Jun 2020 21:17:13: 7000000 INFO @ Sun, 21 Jun 2020 21:17:18: 3000000 INFO @ Sun, 21 Jun 2020 21:17:20: 8000000 INFO @ Sun, 21 Jun 2020 21:17:24: 4000000 INFO @ Sun, 21 Jun 2020 21:17:26: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:17:30: 5000000 INFO @ Sun, 21 Jun 2020 21:17:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:17:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:17:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:17:32: 10000000 INFO @ Sun, 21 Jun 2020 21:17:35: 6000000 INFO @ Sun, 21 Jun 2020 21:17:37: 1000000 INFO @ Sun, 21 Jun 2020 21:17:38: 11000000 INFO @ Sun, 21 Jun 2020 21:17:41: 7000000 INFO @ Sun, 21 Jun 2020 21:17:43: 2000000 INFO @ Sun, 21 Jun 2020 21:17:44: 12000000 INFO @ Sun, 21 Jun 2020 21:17:47: 8000000 INFO @ Sun, 21 Jun 2020 21:17:49: 3000000 INFO @ Sun, 21 Jun 2020 21:17:50: 13000000 INFO @ Sun, 21 Jun 2020 21:17:53: 9000000 INFO @ Sun, 21 Jun 2020 21:17:55: 4000000 INFO @ Sun, 21 Jun 2020 21:17:57: 14000000 INFO @ Sun, 21 Jun 2020 21:17:58: 10000000 INFO @ Sun, 21 Jun 2020 21:18:00: 5000000 INFO @ Sun, 21 Jun 2020 21:18:03: 15000000 INFO @ Sun, 21 Jun 2020 21:18:04: 11000000 INFO @ Sun, 21 Jun 2020 21:18:06: 6000000 INFO @ Sun, 21 Jun 2020 21:18:09: 16000000 INFO @ Sun, 21 Jun 2020 21:18:10: 12000000 INFO @ Sun, 21 Jun 2020 21:18:12: 7000000 INFO @ Sun, 21 Jun 2020 21:18:16: 13000000 INFO @ Sun, 21 Jun 2020 21:18:16: 17000000 INFO @ Sun, 21 Jun 2020 21:18:18: 8000000 INFO @ Sun, 21 Jun 2020 21:18:21: 14000000 INFO @ Sun, 21 Jun 2020 21:18:22: 18000000 INFO @ Sun, 21 Jun 2020 21:18:23: 9000000 INFO @ Sun, 21 Jun 2020 21:18:27: 15000000 INFO @ Sun, 21 Jun 2020 21:18:28: 19000000 INFO @ Sun, 21 Jun 2020 21:18:29: 10000000 INFO @ Sun, 21 Jun 2020 21:18:31: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:18:31: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:18:31: #1 total tags in treatment: 19408225 INFO @ Sun, 21 Jun 2020 21:18:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:18:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:18:32: #1 tags after filtering in treatment: 19408207 INFO @ Sun, 21 Jun 2020 21:18:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:18:32: #1 finished! INFO @ Sun, 21 Jun 2020 21:18:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:18:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:18:33: 16000000 INFO @ Sun, 21 Jun 2020 21:18:33: #2 number of paired peaks: 34 WARNING @ Sun, 21 Jun 2020 21:18:33: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:18:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:18:35: 11000000 INFO @ Sun, 21 Jun 2020 21:18:38: 17000000 INFO @ Sun, 21 Jun 2020 21:18:40: 12000000 INFO @ Sun, 21 Jun 2020 21:18:44: 18000000 INFO @ Sun, 21 Jun 2020 21:18:46: 13000000 INFO @ Sun, 21 Jun 2020 21:18:49: 19000000 INFO @ Sun, 21 Jun 2020 21:18:51: 14000000 INFO @ Sun, 21 Jun 2020 21:18:52: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:18:52: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:18:52: #1 total tags in treatment: 19408225 INFO @ Sun, 21 Jun 2020 21:18:52: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:18:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:18:52: #1 tags after filtering in treatment: 19408207 INFO @ Sun, 21 Jun 2020 21:18:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:18:52: #1 finished! INFO @ Sun, 21 Jun 2020 21:18:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:18:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:18:53: #2 number of paired peaks: 34 WARNING @ Sun, 21 Jun 2020 21:18:53: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:18:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:18:56: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:19:02: 16000000 INFO @ Sun, 21 Jun 2020 21:19:07: 17000000 INFO @ Sun, 21 Jun 2020 21:19:12: 18000000 INFO @ Sun, 21 Jun 2020 21:19:17: 19000000 INFO @ Sun, 21 Jun 2020 21:19:19: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:19:19: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:19:19: #1 total tags in treatment: 19408225 INFO @ Sun, 21 Jun 2020 21:19:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:19:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:19:20: #1 tags after filtering in treatment: 19408207 INFO @ Sun, 21 Jun 2020 21:19:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:19:20: #1 finished! INFO @ Sun, 21 Jun 2020 21:19:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:19:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:19:21: #2 number of paired peaks: 34 WARNING @ Sun, 21 Jun 2020 21:19:21: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:19:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467034/SRX467034.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。