Job ID = 6457618 SRX = SRX467032 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:54:48 prefetch.2.10.7: 1) Downloading 'SRR1164456'... 2020-06-21T11:54:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:00:52 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:00:52 prefetch.2.10.7: 1) 'SRR1164456' was downloaded successfully Read 28454075 spots for SRR1164456/SRR1164456.sra Written 28454075 spots for SRR1164456/SRR1164456.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:57 28454075 reads; of these: 28454075 (100.00%) were unpaired; of these: 1807870 (6.35%) aligned 0 times 24521760 (86.18%) aligned exactly 1 time 2124445 (7.47%) aligned >1 times 93.65% overall alignment rate Time searching: 00:05:57 Overall time: 00:05:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7348460 / 26646205 = 0.2758 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:13:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:13:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:13:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:14:03: 1000000 INFO @ Sun, 21 Jun 2020 21:14:08: 2000000 INFO @ Sun, 21 Jun 2020 21:14:13: 3000000 INFO @ Sun, 21 Jun 2020 21:14:18: 4000000 INFO @ Sun, 21 Jun 2020 21:14:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:14:28: 6000000 INFO @ Sun, 21 Jun 2020 21:14:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:14:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:14:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:14:33: 7000000 INFO @ Sun, 21 Jun 2020 21:14:33: 1000000 INFO @ Sun, 21 Jun 2020 21:14:38: 8000000 INFO @ Sun, 21 Jun 2020 21:14:38: 2000000 INFO @ Sun, 21 Jun 2020 21:14:43: 9000000 INFO @ Sun, 21 Jun 2020 21:14:43: 3000000 INFO @ Sun, 21 Jun 2020 21:14:48: 10000000 INFO @ Sun, 21 Jun 2020 21:14:49: 4000000 INFO @ Sun, 21 Jun 2020 21:14:53: 11000000 INFO @ Sun, 21 Jun 2020 21:14:54: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:14:58: 12000000 INFO @ Sun, 21 Jun 2020 21:14:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:14:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:14:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:14:59: 6000000 INFO @ Sun, 21 Jun 2020 21:15:03: 13000000 INFO @ Sun, 21 Jun 2020 21:15:03: 1000000 INFO @ Sun, 21 Jun 2020 21:15:04: 7000000 INFO @ Sun, 21 Jun 2020 21:15:08: 14000000 INFO @ Sun, 21 Jun 2020 21:15:08: 2000000 INFO @ Sun, 21 Jun 2020 21:15:09: 8000000 INFO @ Sun, 21 Jun 2020 21:15:13: 15000000 INFO @ Sun, 21 Jun 2020 21:15:14: 3000000 INFO @ Sun, 21 Jun 2020 21:15:14: 9000000 INFO @ Sun, 21 Jun 2020 21:15:19: 4000000 INFO @ Sun, 21 Jun 2020 21:15:19: 16000000 INFO @ Sun, 21 Jun 2020 21:15:19: 10000000 INFO @ Sun, 21 Jun 2020 21:15:24: 5000000 INFO @ Sun, 21 Jun 2020 21:15:24: 17000000 INFO @ Sun, 21 Jun 2020 21:15:24: 11000000 INFO @ Sun, 21 Jun 2020 21:15:29: 6000000 INFO @ Sun, 21 Jun 2020 21:15:29: 12000000 INFO @ Sun, 21 Jun 2020 21:15:29: 18000000 INFO @ Sun, 21 Jun 2020 21:15:34: 7000000 INFO @ Sun, 21 Jun 2020 21:15:34: 19000000 INFO @ Sun, 21 Jun 2020 21:15:34: 13000000 INFO @ Sun, 21 Jun 2020 21:15:36: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:15:36: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:15:36: #1 total tags in treatment: 19297745 INFO @ Sun, 21 Jun 2020 21:15:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:15:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:15:37: #1 tags after filtering in treatment: 19297707 INFO @ Sun, 21 Jun 2020 21:15:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:15:37: #1 finished! INFO @ Sun, 21 Jun 2020 21:15:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:15:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:15:38: #2 number of paired peaks: 21 WARNING @ Sun, 21 Jun 2020 21:15:38: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:15:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:15:39: 8000000 INFO @ Sun, 21 Jun 2020 21:15:40: 14000000 INFO @ Sun, 21 Jun 2020 21:15:44: 9000000 INFO @ Sun, 21 Jun 2020 21:15:45: 15000000 INFO @ Sun, 21 Jun 2020 21:15:49: 10000000 INFO @ Sun, 21 Jun 2020 21:15:50: 16000000 INFO @ Sun, 21 Jun 2020 21:15:54: 11000000 INFO @ Sun, 21 Jun 2020 21:15:55: 17000000 INFO @ Sun, 21 Jun 2020 21:15:59: 12000000 INFO @ Sun, 21 Jun 2020 21:16:01: 18000000 INFO @ Sun, 21 Jun 2020 21:16:04: 13000000 INFO @ Sun, 21 Jun 2020 21:16:06: 19000000 INFO @ Sun, 21 Jun 2020 21:16:08: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:16:08: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:16:08: #1 total tags in treatment: 19297745 INFO @ Sun, 21 Jun 2020 21:16:08: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:16:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:16:08: #1 tags after filtering in treatment: 19297707 INFO @ Sun, 21 Jun 2020 21:16:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:16:08: #1 finished! INFO @ Sun, 21 Jun 2020 21:16:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:16:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:16:09: 14000000 INFO @ Sun, 21 Jun 2020 21:16:09: #2 number of paired peaks: 21 WARNING @ Sun, 21 Jun 2020 21:16:09: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:16:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:16:14: 15000000 INFO @ Sun, 21 Jun 2020 21:16:19: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:16:25: 17000000 INFO @ Sun, 21 Jun 2020 21:16:30: 18000000 INFO @ Sun, 21 Jun 2020 21:16:35: 19000000 INFO @ Sun, 21 Jun 2020 21:16:36: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:16:36: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:16:36: #1 total tags in treatment: 19297745 INFO @ Sun, 21 Jun 2020 21:16:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:16:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:16:37: #1 tags after filtering in treatment: 19297707 INFO @ Sun, 21 Jun 2020 21:16:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:16:37: #1 finished! INFO @ Sun, 21 Jun 2020 21:16:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:16:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:16:38: #2 number of paired peaks: 21 WARNING @ Sun, 21 Jun 2020 21:16:38: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:16:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467032/SRX467032.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。