Job ID = 6457613 SRX = SRX467027 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:46:03 prefetch.2.10.7: 1) Downloading 'SRR1164451'... 2020-06-21T11:46:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:50:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:50:26 prefetch.2.10.7: 1) 'SRR1164451' was downloaded successfully Read 28969848 spots for SRR1164451/SRR1164451.sra Written 28969848 spots for SRR1164451/SRR1164451.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:56 28969848 reads; of these: 28969848 (100.00%) were unpaired; of these: 1876572 (6.48%) aligned 0 times 22165318 (76.51%) aligned exactly 1 time 4927958 (17.01%) aligned >1 times 93.52% overall alignment rate Time searching: 00:06:56 Overall time: 00:06:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7430940 / 27093276 = 0.2743 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:04:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:04:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:04:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:05:01: 1000000 INFO @ Sun, 21 Jun 2020 21:05:07: 2000000 INFO @ Sun, 21 Jun 2020 21:05:13: 3000000 INFO @ Sun, 21 Jun 2020 21:05:19: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:05:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:05:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:05:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:05:25: 5000000 INFO @ Sun, 21 Jun 2020 21:05:30: 1000000 INFO @ Sun, 21 Jun 2020 21:05:31: 6000000 INFO @ Sun, 21 Jun 2020 21:05:36: 2000000 INFO @ Sun, 21 Jun 2020 21:05:38: 7000000 INFO @ Sun, 21 Jun 2020 21:05:42: 3000000 INFO @ Sun, 21 Jun 2020 21:05:44: 8000000 INFO @ Sun, 21 Jun 2020 21:05:47: 4000000 INFO @ Sun, 21 Jun 2020 21:05:51: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:05:53: 5000000 INFO @ Sun, 21 Jun 2020 21:05:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:05:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:05:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:05:58: 10000000 INFO @ Sun, 21 Jun 2020 21:05:59: 6000000 INFO @ Sun, 21 Jun 2020 21:06:00: 1000000 INFO @ Sun, 21 Jun 2020 21:06:04: 11000000 INFO @ Sun, 21 Jun 2020 21:06:05: 7000000 INFO @ Sun, 21 Jun 2020 21:06:06: 2000000 INFO @ Sun, 21 Jun 2020 21:06:10: 8000000 INFO @ Sun, 21 Jun 2020 21:06:11: 12000000 INFO @ Sun, 21 Jun 2020 21:06:12: 3000000 INFO @ Sun, 21 Jun 2020 21:06:16: 9000000 INFO @ Sun, 21 Jun 2020 21:06:17: 4000000 INFO @ Sun, 21 Jun 2020 21:06:17: 13000000 INFO @ Sun, 21 Jun 2020 21:06:22: 10000000 INFO @ Sun, 21 Jun 2020 21:06:23: 5000000 INFO @ Sun, 21 Jun 2020 21:06:24: 14000000 INFO @ Sun, 21 Jun 2020 21:06:28: 11000000 INFO @ Sun, 21 Jun 2020 21:06:29: 6000000 INFO @ Sun, 21 Jun 2020 21:06:30: 15000000 INFO @ Sun, 21 Jun 2020 21:06:33: 12000000 INFO @ Sun, 21 Jun 2020 21:06:34: 7000000 INFO @ Sun, 21 Jun 2020 21:06:37: 16000000 INFO @ Sun, 21 Jun 2020 21:06:39: 13000000 INFO @ Sun, 21 Jun 2020 21:06:40: 8000000 INFO @ Sun, 21 Jun 2020 21:06:43: 17000000 INFO @ Sun, 21 Jun 2020 21:06:45: 14000000 INFO @ Sun, 21 Jun 2020 21:06:46: 9000000 INFO @ Sun, 21 Jun 2020 21:06:50: 18000000 INFO @ Sun, 21 Jun 2020 21:06:51: 15000000 INFO @ Sun, 21 Jun 2020 21:06:52: 10000000 INFO @ Sun, 21 Jun 2020 21:06:56: 19000000 INFO @ Sun, 21 Jun 2020 21:06:57: 16000000 INFO @ Sun, 21 Jun 2020 21:06:58: 11000000 INFO @ Sun, 21 Jun 2020 21:07:01: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:07:01: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:07:01: #1 total tags in treatment: 19662336 INFO @ Sun, 21 Jun 2020 21:07:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:07:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:07:01: #1 tags after filtering in treatment: 19662330 INFO @ Sun, 21 Jun 2020 21:07:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:07:01: #1 finished! INFO @ Sun, 21 Jun 2020 21:07:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:07:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:07:03: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 21:07:03: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:07:03: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 21:07:03: 17000000 cut: /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:07:03: 12000000 INFO @ Sun, 21 Jun 2020 21:07:08: 18000000 INFO @ Sun, 21 Jun 2020 21:07:09: 13000000 INFO @ Sun, 21 Jun 2020 21:07:14: 19000000 INFO @ Sun, 21 Jun 2020 21:07:15: 14000000 INFO @ Sun, 21 Jun 2020 21:07:18: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:07:18: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:07:18: #1 total tags in treatment: 19662336 INFO @ Sun, 21 Jun 2020 21:07:18: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:07:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:07:18: #1 tags after filtering in treatment: 19662330 INFO @ Sun, 21 Jun 2020 21:07:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:07:18: #1 finished! INFO @ Sun, 21 Jun 2020 21:07:18: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:07:18: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:07:20: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 21:07:20: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:07:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:07:20: 15000000 INFO @ Sun, 21 Jun 2020 21:07:25: 16000000 INFO @ Sun, 21 Jun 2020 21:07:31: 17000000 INFO @ Sun, 21 Jun 2020 21:07:36: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:07:41: 19000000 INFO @ Sun, 21 Jun 2020 21:07:45: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:07:45: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:07:45: #1 total tags in treatment: 19662336 INFO @ Sun, 21 Jun 2020 21:07:45: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:07:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:07:45: #1 tags after filtering in treatment: 19662330 INFO @ Sun, 21 Jun 2020 21:07:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:07:45: #1 finished! INFO @ Sun, 21 Jun 2020 21:07:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:07:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:07:47: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 21:07:47: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:07:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467027/SRX467027.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。