Job ID = 6457605 SRX = SRX467022 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:02:17 prefetch.2.10.7: 1) Downloading 'SRR1164446'... 2020-06-21T12:02:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:06:00 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:06:00 prefetch.2.10.7: 1) 'SRR1164446' was downloaded successfully Read 30541895 spots for SRR1164446/SRR1164446.sra Written 30541895 spots for SRR1164446/SRR1164446.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:18 30541895 reads; of these: 30541895 (100.00%) were unpaired; of these: 8663359 (28.37%) aligned 0 times 18398878 (60.24%) aligned exactly 1 time 3479658 (11.39%) aligned >1 times 71.63% overall alignment rate Time searching: 00:06:18 Overall time: 00:06:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5427779 / 21878536 = 0.2481 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:18:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:18:47: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:18:47: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:18:52: 1000000 INFO @ Sun, 21 Jun 2020 21:18:57: 2000000 INFO @ Sun, 21 Jun 2020 21:19:02: 3000000 INFO @ Sun, 21 Jun 2020 21:19:07: 4000000 INFO @ Sun, 21 Jun 2020 21:19:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:19:16: 6000000 INFO @ Sun, 21 Jun 2020 21:19:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:19:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:19:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:19:21: 7000000 INFO @ Sun, 21 Jun 2020 21:19:23: 1000000 INFO @ Sun, 21 Jun 2020 21:19:26: 8000000 INFO @ Sun, 21 Jun 2020 21:19:28: 2000000 INFO @ Sun, 21 Jun 2020 21:19:31: 9000000 INFO @ Sun, 21 Jun 2020 21:19:34: 3000000 INFO @ Sun, 21 Jun 2020 21:19:35: 10000000 INFO @ Sun, 21 Jun 2020 21:19:39: 4000000 INFO @ Sun, 21 Jun 2020 21:19:40: 11000000 INFO @ Sun, 21 Jun 2020 21:19:44: 5000000 INFO @ Sun, 21 Jun 2020 21:19:45: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:19:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:19:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:19:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:19:50: 13000000 INFO @ Sun, 21 Jun 2020 21:19:50: 6000000 INFO @ Sun, 21 Jun 2020 21:19:54: 1000000 INFO @ Sun, 21 Jun 2020 21:19:55: 14000000 INFO @ Sun, 21 Jun 2020 21:19:55: 7000000 INFO @ Sun, 21 Jun 2020 21:19:59: 2000000 INFO @ Sun, 21 Jun 2020 21:20:00: 15000000 INFO @ Sun, 21 Jun 2020 21:20:01: 8000000 INFO @ Sun, 21 Jun 2020 21:20:04: 3000000 INFO @ Sun, 21 Jun 2020 21:20:05: 16000000 INFO @ Sun, 21 Jun 2020 21:20:06: 9000000 INFO @ Sun, 21 Jun 2020 21:20:08: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:20:08: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:20:08: #1 total tags in treatment: 16450757 INFO @ Sun, 21 Jun 2020 21:20:08: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:20:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:20:08: #1 tags after filtering in treatment: 16450743 INFO @ Sun, 21 Jun 2020 21:20:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:20:08: #1 finished! INFO @ Sun, 21 Jun 2020 21:20:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:20:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:20:09: 4000000 INFO @ Sun, 21 Jun 2020 21:20:09: #2 number of paired peaks: 62 WARNING @ Sun, 21 Jun 2020 21:20:09: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:20:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:20:11: 10000000 INFO @ Sun, 21 Jun 2020 21:20:14: 5000000 INFO @ Sun, 21 Jun 2020 21:20:17: 11000000 INFO @ Sun, 21 Jun 2020 21:20:18: 6000000 INFO @ Sun, 21 Jun 2020 21:20:23: 12000000 INFO @ Sun, 21 Jun 2020 21:20:23: 7000000 INFO @ Sun, 21 Jun 2020 21:20:28: 8000000 INFO @ Sun, 21 Jun 2020 21:20:28: 13000000 INFO @ Sun, 21 Jun 2020 21:20:33: 9000000 INFO @ Sun, 21 Jun 2020 21:20:35: 14000000 INFO @ Sun, 21 Jun 2020 21:20:38: 10000000 INFO @ Sun, 21 Jun 2020 21:20:41: 15000000 INFO @ Sun, 21 Jun 2020 21:20:43: 11000000 INFO @ Sun, 21 Jun 2020 21:20:47: 16000000 INFO @ Sun, 21 Jun 2020 21:20:48: 12000000 INFO @ Sun, 21 Jun 2020 21:20:50: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:20:50: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:20:50: #1 total tags in treatment: 16450757 INFO @ Sun, 21 Jun 2020 21:20:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:20:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:20:50: #1 tags after filtering in treatment: 16450743 INFO @ Sun, 21 Jun 2020 21:20:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:20:50: #1 finished! INFO @ Sun, 21 Jun 2020 21:20:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:20:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:20:51: #2 number of paired peaks: 62 WARNING @ Sun, 21 Jun 2020 21:20:51: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:20:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 88 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:20:53: 13000000 INFO @ Sun, 21 Jun 2020 21:20:59: 14000000 INFO @ Sun, 21 Jun 2020 21:21:03: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:21:08: 16000000 INFO @ Sun, 21 Jun 2020 21:21:11: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:21:11: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:21:11: #1 total tags in treatment: 16450757 INFO @ Sun, 21 Jun 2020 21:21:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:21:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:21:12: #1 tags after filtering in treatment: 16450743 INFO @ Sun, 21 Jun 2020 21:21:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:21:12: #1 finished! INFO @ Sun, 21 Jun 2020 21:21:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:21:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:21:13: #2 number of paired peaks: 62 WARNING @ Sun, 21 Jun 2020 21:21:13: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:21:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467022/SRX467022.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。