Job ID = 6457604 SRX = SRX467021 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:01:02 prefetch.2.10.7: 1) Downloading 'SRR1164445'... 2020-06-21T12:01:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:05:59 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:05:59 prefetch.2.10.7: 1) 'SRR1164445' was downloaded successfully Read 27172213 spots for SRR1164445/SRR1164445.sra Written 27172213 spots for SRR1164445/SRR1164445.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:36 27172213 reads; of these: 27172213 (100.00%) were unpaired; of these: 10559355 (38.86%) aligned 0 times 15122093 (55.65%) aligned exactly 1 time 1490765 (5.49%) aligned >1 times 61.14% overall alignment rate Time searching: 00:04:37 Overall time: 00:04:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4676341 / 16612858 = 0.2815 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:16:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:16:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:16:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:16:17: 1000000 INFO @ Sun, 21 Jun 2020 21:16:23: 2000000 INFO @ Sun, 21 Jun 2020 21:16:29: 3000000 INFO @ Sun, 21 Jun 2020 21:16:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:16:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:16:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:16:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:16:41: 5000000 INFO @ Sun, 21 Jun 2020 21:16:47: 1000000 INFO @ Sun, 21 Jun 2020 21:16:48: 6000000 INFO @ Sun, 21 Jun 2020 21:16:54: 2000000 INFO @ Sun, 21 Jun 2020 21:16:54: 7000000 INFO @ Sun, 21 Jun 2020 21:17:00: 3000000 INFO @ Sun, 21 Jun 2020 21:17:01: 8000000 INFO @ Sun, 21 Jun 2020 21:17:07: 4000000 INFO @ Sun, 21 Jun 2020 21:17:07: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:17:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:17:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:17:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:17:13: 5000000 INFO @ Sun, 21 Jun 2020 21:17:14: 10000000 INFO @ Sun, 21 Jun 2020 21:17:18: 1000000 INFO @ Sun, 21 Jun 2020 21:17:20: 6000000 INFO @ Sun, 21 Jun 2020 21:17:21: 11000000 INFO @ Sun, 21 Jun 2020 21:17:24: 2000000 INFO @ Sun, 21 Jun 2020 21:17:26: 7000000 INFO @ Sun, 21 Jun 2020 21:17:28: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:17:28: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:17:28: #1 total tags in treatment: 11936517 INFO @ Sun, 21 Jun 2020 21:17:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:17:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:17:28: #1 tags after filtering in treatment: 11936475 INFO @ Sun, 21 Jun 2020 21:17:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:17:28: #1 finished! INFO @ Sun, 21 Jun 2020 21:17:28: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:17:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:17:29: #2 number of paired peaks: 70 WARNING @ Sun, 21 Jun 2020 21:17:29: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:17:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:17:31: 3000000 INFO @ Sun, 21 Jun 2020 21:17:33: 8000000 INFO @ Sun, 21 Jun 2020 21:17:37: 4000000 INFO @ Sun, 21 Jun 2020 21:17:40: 9000000 INFO @ Sun, 21 Jun 2020 21:17:44: 5000000 INFO @ Sun, 21 Jun 2020 21:17:47: 10000000 INFO @ Sun, 21 Jun 2020 21:17:50: 6000000 INFO @ Sun, 21 Jun 2020 21:17:53: 11000000 INFO @ Sun, 21 Jun 2020 21:17:57: 7000000 INFO @ Sun, 21 Jun 2020 21:18:00: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:18:00: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:18:00: #1 total tags in treatment: 11936517 INFO @ Sun, 21 Jun 2020 21:18:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:18:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:18:00: #1 tags after filtering in treatment: 11936475 INFO @ Sun, 21 Jun 2020 21:18:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:18:00: #1 finished! INFO @ Sun, 21 Jun 2020 21:18:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:18:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:18:01: #2 number of paired peaks: 70 WARNING @ Sun, 21 Jun 2020 21:18:01: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:18:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:18:03: 8000000 INFO @ Sun, 21 Jun 2020 21:18:09: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:18:16: 10000000 INFO @ Sun, 21 Jun 2020 21:18:22: 11000000 INFO @ Sun, 21 Jun 2020 21:18:29: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:18:29: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:18:29: #1 total tags in treatment: 11936517 INFO @ Sun, 21 Jun 2020 21:18:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:18:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:18:29: #1 tags after filtering in treatment: 11936475 INFO @ Sun, 21 Jun 2020 21:18:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:18:29: #1 finished! INFO @ Sun, 21 Jun 2020 21:18:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:18:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:18:30: #2 number of paired peaks: 70 WARNING @ Sun, 21 Jun 2020 21:18:30: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:18:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467021/SRX467021.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。