Job ID = 6457598 SRX = SRX467015 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:43:48 prefetch.2.10.7: 1) Downloading 'SRR1164439'... 2020-06-21T11:43:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:50:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:50:14 prefetch.2.10.7: 1) 'SRR1164439' was downloaded successfully Read 43059388 spots for SRR1164439/SRR1164439.sra Written 43059388 spots for SRR1164439/SRR1164439.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:13 43059388 reads; of these: 43059388 (100.00%) were unpaired; of these: 1978361 (4.59%) aligned 0 times 34345158 (79.76%) aligned exactly 1 time 6735869 (15.64%) aligned >1 times 95.41% overall alignment rate Time searching: 00:10:13 Overall time: 00:10:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12256877 / 41081027 = 0.2984 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:11:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:11:03: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:11:03: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:11:08: 1000000 INFO @ Sun, 21 Jun 2020 21:11:13: 2000000 INFO @ Sun, 21 Jun 2020 21:11:18: 3000000 INFO @ Sun, 21 Jun 2020 21:11:23: 4000000 INFO @ Sun, 21 Jun 2020 21:11:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:11:33: 6000000 INFO @ Sun, 21 Jun 2020 21:11:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:11:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:11:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:11:38: 7000000 INFO @ Sun, 21 Jun 2020 21:11:39: 1000000 INFO @ Sun, 21 Jun 2020 21:11:43: 8000000 INFO @ Sun, 21 Jun 2020 21:11:44: 2000000 INFO @ Sun, 21 Jun 2020 21:11:48: 9000000 INFO @ Sun, 21 Jun 2020 21:11:49: 3000000 INFO @ Sun, 21 Jun 2020 21:11:54: 10000000 INFO @ Sun, 21 Jun 2020 21:11:54: 4000000 INFO @ Sun, 21 Jun 2020 21:11:59: 11000000 INFO @ Sun, 21 Jun 2020 21:12:00: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:12:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:12:03: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:12:03: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:12:04: 12000000 INFO @ Sun, 21 Jun 2020 21:12:05: 6000000 INFO @ Sun, 21 Jun 2020 21:12:09: 1000000 INFO @ Sun, 21 Jun 2020 21:12:09: 13000000 INFO @ Sun, 21 Jun 2020 21:12:10: 7000000 INFO @ Sun, 21 Jun 2020 21:12:15: 14000000 INFO @ Sun, 21 Jun 2020 21:12:16: 2000000 INFO @ Sun, 21 Jun 2020 21:12:16: 8000000 INFO @ Sun, 21 Jun 2020 21:12:21: 15000000 INFO @ Sun, 21 Jun 2020 21:12:21: 9000000 INFO @ Sun, 21 Jun 2020 21:12:22: 3000000 INFO @ Sun, 21 Jun 2020 21:12:26: 16000000 INFO @ Sun, 21 Jun 2020 21:12:27: 10000000 INFO @ Sun, 21 Jun 2020 21:12:28: 4000000 INFO @ Sun, 21 Jun 2020 21:12:32: 17000000 INFO @ Sun, 21 Jun 2020 21:12:33: 11000000 INFO @ Sun, 21 Jun 2020 21:12:34: 5000000 INFO @ Sun, 21 Jun 2020 21:12:37: 18000000 INFO @ Sun, 21 Jun 2020 21:12:38: 12000000 INFO @ Sun, 21 Jun 2020 21:12:40: 6000000 INFO @ Sun, 21 Jun 2020 21:12:43: 19000000 INFO @ Sun, 21 Jun 2020 21:12:44: 13000000 INFO @ Sun, 21 Jun 2020 21:12:46: 7000000 INFO @ Sun, 21 Jun 2020 21:12:49: 20000000 INFO @ Sun, 21 Jun 2020 21:12:49: 14000000 INFO @ Sun, 21 Jun 2020 21:12:52: 8000000 INFO @ Sun, 21 Jun 2020 21:12:54: 21000000 INFO @ Sun, 21 Jun 2020 21:12:55: 15000000 INFO @ Sun, 21 Jun 2020 21:12:59: 9000000 INFO @ Sun, 21 Jun 2020 21:13:00: 22000000 INFO @ Sun, 21 Jun 2020 21:13:01: 16000000 INFO @ Sun, 21 Jun 2020 21:13:05: 10000000 INFO @ Sun, 21 Jun 2020 21:13:06: 23000000 INFO @ Sun, 21 Jun 2020 21:13:06: 17000000 INFO @ Sun, 21 Jun 2020 21:13:11: 11000000 INFO @ Sun, 21 Jun 2020 21:13:11: 24000000 INFO @ Sun, 21 Jun 2020 21:13:12: 18000000 INFO @ Sun, 21 Jun 2020 21:13:17: 25000000 INFO @ Sun, 21 Jun 2020 21:13:17: 12000000 INFO @ Sun, 21 Jun 2020 21:13:17: 19000000 INFO @ Sun, 21 Jun 2020 21:13:22: 26000000 INFO @ Sun, 21 Jun 2020 21:13:23: 20000000 INFO @ Sun, 21 Jun 2020 21:13:23: 13000000 INFO @ Sun, 21 Jun 2020 21:13:28: 27000000 INFO @ Sun, 21 Jun 2020 21:13:28: 21000000 INFO @ Sun, 21 Jun 2020 21:13:30: 14000000 INFO @ Sun, 21 Jun 2020 21:13:33: 28000000 INFO @ Sun, 21 Jun 2020 21:13:34: 22000000 INFO @ Sun, 21 Jun 2020 21:13:36: 15000000 INFO @ Sun, 21 Jun 2020 21:13:38: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:13:38: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:13:38: #1 total tags in treatment: 28824150 INFO @ Sun, 21 Jun 2020 21:13:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:13:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:13:39: #1 tags after filtering in treatment: 28824146 INFO @ Sun, 21 Jun 2020 21:13:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:13:39: #1 finished! INFO @ Sun, 21 Jun 2020 21:13:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:13:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:13:40: 23000000 INFO @ Sun, 21 Jun 2020 21:13:41: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:13:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:13:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:13:42: 16000000 INFO @ Sun, 21 Jun 2020 21:13:45: 24000000 INFO @ Sun, 21 Jun 2020 21:13:48: 17000000 INFO @ Sun, 21 Jun 2020 21:13:51: 25000000 INFO @ Sun, 21 Jun 2020 21:13:55: 18000000 INFO @ Sun, 21 Jun 2020 21:13:57: 26000000 INFO @ Sun, 21 Jun 2020 21:14:01: 19000000 INFO @ Sun, 21 Jun 2020 21:14:02: 27000000 INFO @ Sun, 21 Jun 2020 21:14:07: 20000000 INFO @ Sun, 21 Jun 2020 21:14:08: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:14:13: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:14:13: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:14:13: #1 total tags in treatment: 28824150 INFO @ Sun, 21 Jun 2020 21:14:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:14:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:14:13: 21000000 INFO @ Sun, 21 Jun 2020 21:14:13: #1 tags after filtering in treatment: 28824146 INFO @ Sun, 21 Jun 2020 21:14:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:14:13: #1 finished! INFO @ Sun, 21 Jun 2020 21:14:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:14:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:14:15: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:14:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:14:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:14:19: 22000000 INFO @ Sun, 21 Jun 2020 21:14:25: 23000000 INFO @ Sun, 21 Jun 2020 21:14:31: 24000000 INFO @ Sun, 21 Jun 2020 21:14:37: 25000000 INFO @ Sun, 21 Jun 2020 21:14:43: 26000000 INFO @ Sun, 21 Jun 2020 21:14:49: 27000000 INFO @ Sun, 21 Jun 2020 21:14:55: 28000000 INFO @ Sun, 21 Jun 2020 21:15:00: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:15:00: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:15:00: #1 total tags in treatment: 28824150 INFO @ Sun, 21 Jun 2020 21:15:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:15:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:15:01: #1 tags after filtering in treatment: 28824146 INFO @ Sun, 21 Jun 2020 21:15:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:15:01: #1 finished! INFO @ Sun, 21 Jun 2020 21:15:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:15:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:15:03: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:15:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:15:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467015/SRX467015.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。