Job ID = 6457597 SRX = SRX467014 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:41:25 prefetch.2.10.7: 1) Downloading 'SRR1164438'... 2020-06-21T11:41:25 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:47:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:47:26 prefetch.2.10.7: 1) 'SRR1164438' was downloaded successfully Read 39940498 spots for SRR1164438/SRR1164438.sra Written 39940498 spots for SRR1164438/SRR1164438.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:17 39940498 reads; of these: 39940498 (100.00%) were unpaired; of these: 10771381 (26.97%) aligned 0 times 25750644 (64.47%) aligned exactly 1 time 3418473 (8.56%) aligned >1 times 73.03% overall alignment rate Time searching: 00:07:17 Overall time: 00:07:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8938494 / 29169117 = 0.3064 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:02:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:02:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:02:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:02:29: 1000000 INFO @ Sun, 21 Jun 2020 21:02:34: 2000000 INFO @ Sun, 21 Jun 2020 21:02:39: 3000000 INFO @ Sun, 21 Jun 2020 21:02:44: 4000000 INFO @ Sun, 21 Jun 2020 21:02:49: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:02:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:02:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:02:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:02:54: 6000000 INFO @ Sun, 21 Jun 2020 21:02:58: 1000000 INFO @ Sun, 21 Jun 2020 21:03:00: 7000000 INFO @ Sun, 21 Jun 2020 21:03:04: 2000000 INFO @ Sun, 21 Jun 2020 21:03:05: 8000000 INFO @ Sun, 21 Jun 2020 21:03:09: 3000000 INFO @ Sun, 21 Jun 2020 21:03:10: 9000000 INFO @ Sun, 21 Jun 2020 21:03:15: 4000000 INFO @ Sun, 21 Jun 2020 21:03:16: 10000000 INFO @ Sun, 21 Jun 2020 21:03:21: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:03:21: 11000000 INFO @ Sun, 21 Jun 2020 21:03:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:03:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:03:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:03:27: 6000000 INFO @ Sun, 21 Jun 2020 21:03:27: 12000000 INFO @ Sun, 21 Jun 2020 21:03:29: 1000000 INFO @ Sun, 21 Jun 2020 21:03:33: 7000000 INFO @ Sun, 21 Jun 2020 21:03:33: 13000000 INFO @ Sun, 21 Jun 2020 21:03:36: 2000000 INFO @ Sun, 21 Jun 2020 21:03:38: 8000000 INFO @ Sun, 21 Jun 2020 21:03:39: 14000000 INFO @ Sun, 21 Jun 2020 21:03:42: 3000000 INFO @ Sun, 21 Jun 2020 21:03:44: 9000000 INFO @ Sun, 21 Jun 2020 21:03:44: 15000000 INFO @ Sun, 21 Jun 2020 21:03:49: 4000000 INFO @ Sun, 21 Jun 2020 21:03:50: 16000000 INFO @ Sun, 21 Jun 2020 21:03:50: 10000000 INFO @ Sun, 21 Jun 2020 21:03:55: 5000000 INFO @ Sun, 21 Jun 2020 21:03:56: 11000000 INFO @ Sun, 21 Jun 2020 21:03:56: 17000000 INFO @ Sun, 21 Jun 2020 21:04:02: 6000000 INFO @ Sun, 21 Jun 2020 21:04:02: 12000000 INFO @ Sun, 21 Jun 2020 21:04:02: 18000000 INFO @ Sun, 21 Jun 2020 21:04:07: 13000000 INFO @ Sun, 21 Jun 2020 21:04:08: 19000000 INFO @ Sun, 21 Jun 2020 21:04:08: 7000000 INFO @ Sun, 21 Jun 2020 21:04:13: 14000000 INFO @ Sun, 21 Jun 2020 21:04:13: 20000000 INFO @ Sun, 21 Jun 2020 21:04:14: 8000000 INFO @ Sun, 21 Jun 2020 21:04:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:04:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:04:15: #1 total tags in treatment: 20230623 INFO @ Sun, 21 Jun 2020 21:04:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:04:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:04:15: #1 tags after filtering in treatment: 20230598 INFO @ Sun, 21 Jun 2020 21:04:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:04:15: #1 finished! INFO @ Sun, 21 Jun 2020 21:04:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:04:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:04:17: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 21:04:17: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:04:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:04:19: 15000000 INFO @ Sun, 21 Jun 2020 21:04:20: 9000000 INFO @ Sun, 21 Jun 2020 21:04:25: 16000000 INFO @ Sun, 21 Jun 2020 21:04:27: 10000000 INFO @ Sun, 21 Jun 2020 21:04:31: 17000000 INFO @ Sun, 21 Jun 2020 21:04:33: 11000000 INFO @ Sun, 21 Jun 2020 21:04:37: 18000000 INFO @ Sun, 21 Jun 2020 21:04:39: 12000000 INFO @ Sun, 21 Jun 2020 21:04:42: 19000000 INFO @ Sun, 21 Jun 2020 21:04:45: 13000000 INFO @ Sun, 21 Jun 2020 21:04:48: 20000000 INFO @ Sun, 21 Jun 2020 21:04:50: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:04:50: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:04:50: #1 total tags in treatment: 20230623 INFO @ Sun, 21 Jun 2020 21:04:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:04:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:04:50: #1 tags after filtering in treatment: 20230598 INFO @ Sun, 21 Jun 2020 21:04:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:04:50: #1 finished! INFO @ Sun, 21 Jun 2020 21:04:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:04:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:04:51: 14000000 INFO @ Sun, 21 Jun 2020 21:04:52: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 21:04:52: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:04:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:04:57: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:05:03: 16000000 INFO @ Sun, 21 Jun 2020 21:05:09: 17000000 INFO @ Sun, 21 Jun 2020 21:05:15: 18000000 INFO @ Sun, 21 Jun 2020 21:05:21: 19000000 INFO @ Sun, 21 Jun 2020 21:05:27: 20000000 INFO @ Sun, 21 Jun 2020 21:05:28: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:05:28: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:05:28: #1 total tags in treatment: 20230623 INFO @ Sun, 21 Jun 2020 21:05:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:05:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:05:29: #1 tags after filtering in treatment: 20230598 INFO @ Sun, 21 Jun 2020 21:05:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:05:29: #1 finished! INFO @ Sun, 21 Jun 2020 21:05:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:05:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:05:30: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 21:05:30: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:05:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467014/SRX467014.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。