Job ID = 6457596 SRX = SRX467013 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:39:40 prefetch.2.10.7: 1) Downloading 'SRR1164437'... 2020-06-21T11:39:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:49:53 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:49:53 prefetch.2.10.7: 1) 'SRR1164437' was downloaded successfully Read 60880063 spots for SRR1164437/SRR1164437.sra Written 60880063 spots for SRR1164437/SRR1164437.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:37 60880063 reads; of these: 60880063 (100.00%) were unpaired; of these: 27122452 (44.55%) aligned 0 times 30144073 (49.51%) aligned exactly 1 time 3613538 (5.94%) aligned >1 times 55.45% overall alignment rate Time searching: 00:09:37 Overall time: 00:09:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 11539318 / 33757611 = 0.3418 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:09:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:09:03: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:09:03: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:09:09: 1000000 INFO @ Sun, 21 Jun 2020 21:09:14: 2000000 INFO @ Sun, 21 Jun 2020 21:09:19: 3000000 INFO @ Sun, 21 Jun 2020 21:09:25: 4000000 INFO @ Sun, 21 Jun 2020 21:09:30: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:09:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:09:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:09:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:09:36: 6000000 INFO @ Sun, 21 Jun 2020 21:09:40: 1000000 INFO @ Sun, 21 Jun 2020 21:09:42: 7000000 INFO @ Sun, 21 Jun 2020 21:09:46: 2000000 INFO @ Sun, 21 Jun 2020 21:09:48: 8000000 INFO @ Sun, 21 Jun 2020 21:09:53: 3000000 INFO @ Sun, 21 Jun 2020 21:09:54: 9000000 INFO @ Sun, 21 Jun 2020 21:10:00: 10000000 INFO @ Sun, 21 Jun 2020 21:10:00: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:10:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:10:03: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:10:03: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:10:06: 11000000 INFO @ Sun, 21 Jun 2020 21:10:07: 5000000 INFO @ Sun, 21 Jun 2020 21:10:10: 1000000 INFO @ Sun, 21 Jun 2020 21:10:12: 12000000 INFO @ Sun, 21 Jun 2020 21:10:14: 6000000 INFO @ Sun, 21 Jun 2020 21:10:17: 2000000 INFO @ Sun, 21 Jun 2020 21:10:18: 13000000 INFO @ Sun, 21 Jun 2020 21:10:20: 7000000 INFO @ Sun, 21 Jun 2020 21:10:23: 3000000 INFO @ Sun, 21 Jun 2020 21:10:24: 14000000 INFO @ Sun, 21 Jun 2020 21:10:27: 8000000 INFO @ Sun, 21 Jun 2020 21:10:30: 4000000 INFO @ Sun, 21 Jun 2020 21:10:30: 15000000 INFO @ Sun, 21 Jun 2020 21:10:34: 9000000 INFO @ Sun, 21 Jun 2020 21:10:36: 16000000 INFO @ Sun, 21 Jun 2020 21:10:36: 5000000 INFO @ Sun, 21 Jun 2020 21:10:41: 10000000 INFO @ Sun, 21 Jun 2020 21:10:42: 17000000 INFO @ Sun, 21 Jun 2020 21:10:43: 6000000 INFO @ Sun, 21 Jun 2020 21:10:48: 11000000 INFO @ Sun, 21 Jun 2020 21:10:49: 18000000 INFO @ Sun, 21 Jun 2020 21:10:49: 7000000 INFO @ Sun, 21 Jun 2020 21:10:54: 12000000 INFO @ Sun, 21 Jun 2020 21:10:55: 19000000 INFO @ Sun, 21 Jun 2020 21:10:56: 8000000 INFO @ Sun, 21 Jun 2020 21:11:01: 13000000 INFO @ Sun, 21 Jun 2020 21:11:01: 20000000 INFO @ Sun, 21 Jun 2020 21:11:02: 9000000 INFO @ Sun, 21 Jun 2020 21:11:07: 21000000 INFO @ Sun, 21 Jun 2020 21:11:08: 14000000 INFO @ Sun, 21 Jun 2020 21:11:09: 10000000 INFO @ Sun, 21 Jun 2020 21:11:14: 22000000 INFO @ Sun, 21 Jun 2020 21:11:15: 15000000 INFO @ Sun, 21 Jun 2020 21:11:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:11:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:11:15: #1 total tags in treatment: 22218293 INFO @ Sun, 21 Jun 2020 21:11:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:11:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:11:16: 11000000 INFO @ Sun, 21 Jun 2020 21:11:16: #1 tags after filtering in treatment: 22218274 INFO @ Sun, 21 Jun 2020 21:11:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:11:16: #1 finished! INFO @ Sun, 21 Jun 2020 21:11:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:11:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:11:17: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 21:11:17: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:11:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:11:21: 16000000 INFO @ Sun, 21 Jun 2020 21:11:22: 12000000 INFO @ Sun, 21 Jun 2020 21:11:28: 17000000 INFO @ Sun, 21 Jun 2020 21:11:28: 13000000 INFO @ Sun, 21 Jun 2020 21:11:35: 18000000 INFO @ Sun, 21 Jun 2020 21:11:35: 14000000 INFO @ Sun, 21 Jun 2020 21:11:42: 15000000 INFO @ Sun, 21 Jun 2020 21:11:42: 19000000 INFO @ Sun, 21 Jun 2020 21:11:49: 20000000 INFO @ Sun, 21 Jun 2020 21:11:49: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:11:55: 17000000 INFO @ Sun, 21 Jun 2020 21:11:55: 21000000 INFO @ Sun, 21 Jun 2020 21:12:02: 18000000 INFO @ Sun, 21 Jun 2020 21:12:02: 22000000 INFO @ Sun, 21 Jun 2020 21:12:04: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:12:04: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:12:04: #1 total tags in treatment: 22218293 INFO @ Sun, 21 Jun 2020 21:12:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:12:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:12:05: #1 tags after filtering in treatment: 22218274 INFO @ Sun, 21 Jun 2020 21:12:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:12:05: #1 finished! INFO @ Sun, 21 Jun 2020 21:12:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:12:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:12:06: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 21:12:06: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:12:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:12:08: 19000000 INFO @ Sun, 21 Jun 2020 21:12:14: 20000000 INFO @ Sun, 21 Jun 2020 21:12:19: 21000000 INFO @ Sun, 21 Jun 2020 21:12:25: 22000000 INFO @ Sun, 21 Jun 2020 21:12:26: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:12:26: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:12:26: #1 total tags in treatment: 22218293 INFO @ Sun, 21 Jun 2020 21:12:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:12:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:12:27: #1 tags after filtering in treatment: 22218274 INFO @ Sun, 21 Jun 2020 21:12:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:12:27: #1 finished! INFO @ Sun, 21 Jun 2020 21:12:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:12:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:12:28: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 21:12:28: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:12:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467013/SRX467013.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。