Job ID = 6457592 SRX = SRX467012 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:13:04 prefetch.2.10.7: 1) Downloading 'SRR1164436'... 2020-06-21T12:13:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:15:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:15:27 prefetch.2.10.7: 'SRR1164436' is valid 2020-06-21T12:15:27 prefetch.2.10.7: 1) 'SRR1164436' was downloaded successfully Read 17374320 spots for SRR1164436/SRR1164436.sra Written 17374320 spots for SRR1164436/SRR1164436.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:59 17374320 reads; of these: 17374320 (100.00%) were unpaired; of these: 1311031 (7.55%) aligned 0 times 14092754 (81.11%) aligned exactly 1 time 1970535 (11.34%) aligned >1 times 92.45% overall alignment rate Time searching: 00:03:59 Overall time: 00:03:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2610806 / 16063289 = 0.1625 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:25:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:25:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:25:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:25:17: 1000000 INFO @ Sun, 21 Jun 2020 21:25:22: 2000000 INFO @ Sun, 21 Jun 2020 21:25:27: 3000000 INFO @ Sun, 21 Jun 2020 21:25:32: 4000000 INFO @ Sun, 21 Jun 2020 21:25:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:25:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:25:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:25:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:25:42: 6000000 INFO @ Sun, 21 Jun 2020 21:25:47: 1000000 INFO @ Sun, 21 Jun 2020 21:25:48: 7000000 INFO @ Sun, 21 Jun 2020 21:25:53: 2000000 INFO @ Sun, 21 Jun 2020 21:25:53: 8000000 INFO @ Sun, 21 Jun 2020 21:25:58: 3000000 INFO @ Sun, 21 Jun 2020 21:25:59: 9000000 INFO @ Sun, 21 Jun 2020 21:26:04: 4000000 INFO @ Sun, 21 Jun 2020 21:26:04: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:26:10: 5000000 INFO @ Sun, 21 Jun 2020 21:26:10: 11000000 INFO @ Sun, 21 Jun 2020 21:26:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:26:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:26:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:26:16: 6000000 INFO @ Sun, 21 Jun 2020 21:26:17: 12000000 INFO @ Sun, 21 Jun 2020 21:26:18: 1000000 INFO @ Sun, 21 Jun 2020 21:26:22: 7000000 INFO @ Sun, 21 Jun 2020 21:26:23: 13000000 INFO @ Sun, 21 Jun 2020 21:26:24: 2000000 INFO @ Sun, 21 Jun 2020 21:26:26: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:26:26: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:26:26: #1 total tags in treatment: 13452483 INFO @ Sun, 21 Jun 2020 21:26:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:26:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:26:27: #1 tags after filtering in treatment: 13452466 INFO @ Sun, 21 Jun 2020 21:26:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:26:27: #1 finished! INFO @ Sun, 21 Jun 2020 21:26:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:26:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:26:28: 8000000 INFO @ Sun, 21 Jun 2020 21:26:28: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 21:26:28: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:26:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:26:29: 3000000 INFO @ Sun, 21 Jun 2020 21:26:33: 9000000 INFO @ Sun, 21 Jun 2020 21:26:35: 4000000 INFO @ Sun, 21 Jun 2020 21:26:39: 10000000 INFO @ Sun, 21 Jun 2020 21:26:40: 5000000 INFO @ Sun, 21 Jun 2020 21:26:44: 11000000 INFO @ Sun, 21 Jun 2020 21:26:46: 6000000 INFO @ Sun, 21 Jun 2020 21:26:51: 12000000 INFO @ Sun, 21 Jun 2020 21:26:51: 7000000 INFO @ Sun, 21 Jun 2020 21:26:56: 13000000 INFO @ Sun, 21 Jun 2020 21:26:57: 8000000 INFO @ Sun, 21 Jun 2020 21:26:59: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:26:59: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:26:59: #1 total tags in treatment: 13452483 INFO @ Sun, 21 Jun 2020 21:26:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:26:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:27:00: #1 tags after filtering in treatment: 13452466 INFO @ Sun, 21 Jun 2020 21:27:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:27:00: #1 finished! INFO @ Sun, 21 Jun 2020 21:27:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:27:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:27:01: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 21:27:01: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:27:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:27:02: 9000000 INFO @ Sun, 21 Jun 2020 21:27:07: 10000000 INFO @ Sun, 21 Jun 2020 21:27:12: 11000000 INFO @ Sun, 21 Jun 2020 21:27:18: 12000000 INFO @ Sun, 21 Jun 2020 21:27:23: 13000000 INFO @ Sun, 21 Jun 2020 21:27:25: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:27:25: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:27:25: #1 total tags in treatment: 13452483 INFO @ Sun, 21 Jun 2020 21:27:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:27:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:27:26: #1 tags after filtering in treatment: 13452466 INFO @ Sun, 21 Jun 2020 21:27:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:27:26: #1 finished! INFO @ Sun, 21 Jun 2020 21:27:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:27:26: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:27:27: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 21:27:27: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:27:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467012/SRX467012.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。