Job ID = 6457591 SRX = SRX467011 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:46:03 prefetch.2.10.7: 1) Downloading 'SRR1164435'... 2020-06-21T11:46:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:48:43 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:48:45 prefetch.2.10.7: 'SRR1164435' is valid 2020-06-21T11:48:45 prefetch.2.10.7: 1) 'SRR1164435' was downloaded successfully Read 17374320 spots for SRR1164435/SRR1164435.sra Written 17374320 spots for SRR1164435/SRR1164435.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:55 17374320 reads; of these: 17374320 (100.00%) were unpaired; of these: 1311047 (7.55%) aligned 0 times 14092690 (81.11%) aligned exactly 1 time 1970583 (11.34%) aligned >1 times 92.45% overall alignment rate Time searching: 00:03:55 Overall time: 00:03:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2610764 / 16063273 = 0.1625 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:57:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:57:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:57:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:57:28: 1000000 INFO @ Sun, 21 Jun 2020 20:57:33: 2000000 INFO @ Sun, 21 Jun 2020 20:57:37: 3000000 INFO @ Sun, 21 Jun 2020 20:57:42: 4000000 INFO @ Sun, 21 Jun 2020 20:57:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:57:51: 6000000 INFO @ Sun, 21 Jun 2020 20:57:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:57:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:57:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:57:56: 7000000 INFO @ Sun, 21 Jun 2020 20:57:59: 1000000 INFO @ Sun, 21 Jun 2020 20:58:01: 8000000 INFO @ Sun, 21 Jun 2020 20:58:05: 2000000 INFO @ Sun, 21 Jun 2020 20:58:06: 9000000 INFO @ Sun, 21 Jun 2020 20:58:11: 3000000 INFO @ Sun, 21 Jun 2020 20:58:11: 10000000 INFO @ Sun, 21 Jun 2020 20:58:16: 11000000 INFO @ Sun, 21 Jun 2020 20:58:17: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:58:22: 12000000 INFO @ Sun, 21 Jun 2020 20:58:23: 5000000 INFO @ Sun, 21 Jun 2020 20:58:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:58:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:58:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:58:27: 13000000 INFO @ Sun, 21 Jun 2020 20:58:28: 1000000 INFO @ Sun, 21 Jun 2020 20:58:29: 6000000 INFO @ Sun, 21 Jun 2020 20:58:29: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:58:29: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:58:29: #1 total tags in treatment: 13452509 INFO @ Sun, 21 Jun 2020 20:58:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:58:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:58:30: #1 tags after filtering in treatment: 13452489 INFO @ Sun, 21 Jun 2020 20:58:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:58:30: #1 finished! INFO @ Sun, 21 Jun 2020 20:58:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:58:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:58:31: #2 number of paired peaks: 37 WARNING @ Sun, 21 Jun 2020 20:58:31: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:58:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:58:33: 2000000 INFO @ Sun, 21 Jun 2020 20:58:35: 7000000 INFO @ Sun, 21 Jun 2020 20:58:38: 3000000 INFO @ Sun, 21 Jun 2020 20:58:41: 8000000 INFO @ Sun, 21 Jun 2020 20:58:43: 4000000 INFO @ Sun, 21 Jun 2020 20:58:47: 9000000 INFO @ Sun, 21 Jun 2020 20:58:48: 5000000 INFO @ Sun, 21 Jun 2020 20:58:53: 10000000 INFO @ Sun, 21 Jun 2020 20:58:53: 6000000 INFO @ Sun, 21 Jun 2020 20:58:58: 7000000 INFO @ Sun, 21 Jun 2020 20:58:59: 11000000 INFO @ Sun, 21 Jun 2020 20:59:03: 8000000 INFO @ Sun, 21 Jun 2020 20:59:05: 12000000 INFO @ Sun, 21 Jun 2020 20:59:09: 9000000 INFO @ Sun, 21 Jun 2020 20:59:11: 13000000 INFO @ Sun, 21 Jun 2020 20:59:14: 10000000 INFO @ Sun, 21 Jun 2020 20:59:14: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:59:14: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:59:14: #1 total tags in treatment: 13452509 INFO @ Sun, 21 Jun 2020 20:59:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:59:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:59:15: #1 tags after filtering in treatment: 13452489 INFO @ Sun, 21 Jun 2020 20:59:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:59:15: #1 finished! INFO @ Sun, 21 Jun 2020 20:59:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:59:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:59:16: #2 number of paired peaks: 37 WARNING @ Sun, 21 Jun 2020 20:59:16: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:59:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:59:19: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:59:25: 12000000 INFO @ Sun, 21 Jun 2020 20:59:30: 13000000 INFO @ Sun, 21 Jun 2020 20:59:32: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:59:32: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:59:32: #1 total tags in treatment: 13452509 INFO @ Sun, 21 Jun 2020 20:59:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:59:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:59:33: #1 tags after filtering in treatment: 13452489 INFO @ Sun, 21 Jun 2020 20:59:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:59:33: #1 finished! INFO @ Sun, 21 Jun 2020 20:59:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:59:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:59:34: #2 number of paired peaks: 37 WARNING @ Sun, 21 Jun 2020 20:59:34: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:59:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX467011/SRX467011.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。