Job ID = 6457590 SRX = SRX4669069 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:38:55 prefetch.2.10.7: 1) Downloading 'SRR7817594'... 2020-06-21T11:38:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:42:34 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:42:35 prefetch.2.10.7: 'SRR7817594' is valid 2020-06-21T11:42:35 prefetch.2.10.7: 1) 'SRR7817594' was downloaded successfully 2020-06-21T11:42:35 prefetch.2.10.7: 'SRR7817594' has 0 unresolved dependencies Read 29334226 spots for SRR7817594/SRR7817594.sra Written 29334226 spots for SRR7817594/SRR7817594.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:26 29334226 reads; of these: 29334226 (100.00%) were unpaired; of these: 1279925 (4.36%) aligned 0 times 25503946 (86.94%) aligned exactly 1 time 2550355 (8.69%) aligned >1 times 95.64% overall alignment rate Time searching: 00:06:26 Overall time: 00:06:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7075716 / 28054301 = 0.2522 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:55:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:55:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:55:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:55:35: 1000000 INFO @ Sun, 21 Jun 2020 20:55:40: 2000000 INFO @ Sun, 21 Jun 2020 20:55:46: 3000000 INFO @ Sun, 21 Jun 2020 20:55:51: 4000000 INFO @ Sun, 21 Jun 2020 20:55:57: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:56:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:56:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:56:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:56:02: 6000000 INFO @ Sun, 21 Jun 2020 20:56:05: 1000000 INFO @ Sun, 21 Jun 2020 20:56:07: 7000000 INFO @ Sun, 21 Jun 2020 20:56:11: 2000000 INFO @ Sun, 21 Jun 2020 20:56:13: 8000000 INFO @ Sun, 21 Jun 2020 20:56:16: 3000000 INFO @ Sun, 21 Jun 2020 20:56:18: 9000000 INFO @ Sun, 21 Jun 2020 20:56:22: 4000000 INFO @ Sun, 21 Jun 2020 20:56:24: 10000000 INFO @ Sun, 21 Jun 2020 20:56:27: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:56:29: 11000000 INFO @ Sun, 21 Jun 2020 20:56:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:56:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:56:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:56:32: 6000000 INFO @ Sun, 21 Jun 2020 20:56:35: 12000000 INFO @ Sun, 21 Jun 2020 20:56:35: 1000000 INFO @ Sun, 21 Jun 2020 20:56:38: 7000000 INFO @ Sun, 21 Jun 2020 20:56:40: 13000000 INFO @ Sun, 21 Jun 2020 20:56:41: 2000000 INFO @ Sun, 21 Jun 2020 20:56:43: 8000000 INFO @ Sun, 21 Jun 2020 20:56:46: 14000000 INFO @ Sun, 21 Jun 2020 20:56:46: 3000000 INFO @ Sun, 21 Jun 2020 20:56:49: 9000000 INFO @ Sun, 21 Jun 2020 20:56:51: 15000000 INFO @ Sun, 21 Jun 2020 20:56:52: 4000000 INFO @ Sun, 21 Jun 2020 20:56:54: 10000000 INFO @ Sun, 21 Jun 2020 20:56:56: 16000000 INFO @ Sun, 21 Jun 2020 20:56:57: 5000000 INFO @ Sun, 21 Jun 2020 20:56:59: 11000000 INFO @ Sun, 21 Jun 2020 20:57:02: 17000000 INFO @ Sun, 21 Jun 2020 20:57:03: 6000000 INFO @ Sun, 21 Jun 2020 20:57:05: 12000000 INFO @ Sun, 21 Jun 2020 20:57:07: 18000000 INFO @ Sun, 21 Jun 2020 20:57:08: 7000000 INFO @ Sun, 21 Jun 2020 20:57:10: 13000000 INFO @ Sun, 21 Jun 2020 20:57:13: 19000000 INFO @ Sun, 21 Jun 2020 20:57:13: 8000000 INFO @ Sun, 21 Jun 2020 20:57:16: 14000000 INFO @ Sun, 21 Jun 2020 20:57:18: 20000000 INFO @ Sun, 21 Jun 2020 20:57:19: 9000000 INFO @ Sun, 21 Jun 2020 20:57:21: 15000000 INFO @ Sun, 21 Jun 2020 20:57:24: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:57:24: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:57:24: #1 total tags in treatment: 20978585 INFO @ Sun, 21 Jun 2020 20:57:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:57:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:57:24: 10000000 INFO @ Sun, 21 Jun 2020 20:57:24: #1 tags after filtering in treatment: 20978547 INFO @ Sun, 21 Jun 2020 20:57:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:57:24: #1 finished! INFO @ Sun, 21 Jun 2020 20:57:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:57:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:57:26: #2 number of paired peaks: 63 WARNING @ Sun, 21 Jun 2020 20:57:26: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:57:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:57:26: 16000000 INFO @ Sun, 21 Jun 2020 20:57:30: 11000000 INFO @ Sun, 21 Jun 2020 20:57:32: 17000000 INFO @ Sun, 21 Jun 2020 20:57:35: 12000000 INFO @ Sun, 21 Jun 2020 20:57:37: 18000000 INFO @ Sun, 21 Jun 2020 20:57:41: 13000000 INFO @ Sun, 21 Jun 2020 20:57:43: 19000000 INFO @ Sun, 21 Jun 2020 20:57:46: 14000000 INFO @ Sun, 21 Jun 2020 20:57:48: 20000000 INFO @ Sun, 21 Jun 2020 20:57:51: 15000000 INFO @ Sun, 21 Jun 2020 20:57:53: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:57:53: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:57:53: #1 total tags in treatment: 20978585 INFO @ Sun, 21 Jun 2020 20:57:53: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:57:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:57:54: #1 tags after filtering in treatment: 20978547 INFO @ Sun, 21 Jun 2020 20:57:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:57:54: #1 finished! INFO @ Sun, 21 Jun 2020 20:57:54: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:57:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:57:55: #2 number of paired peaks: 63 WARNING @ Sun, 21 Jun 2020 20:57:55: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:57:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:57:56: 16000000 INFO @ Sun, 21 Jun 2020 20:58:01: 17000000 INFO @ Sun, 21 Jun 2020 20:58:07: 18000000 INFO @ Sun, 21 Jun 2020 20:58:12: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:58:17: 20000000 INFO @ Sun, 21 Jun 2020 20:58:23: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:58:23: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:58:23: #1 total tags in treatment: 20978585 INFO @ Sun, 21 Jun 2020 20:58:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:58:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:58:23: #1 tags after filtering in treatment: 20978547 INFO @ Sun, 21 Jun 2020 20:58:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:58:23: #1 finished! INFO @ Sun, 21 Jun 2020 20:58:23: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:58:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:58:25: #2 number of paired peaks: 63 WARNING @ Sun, 21 Jun 2020 20:58:25: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:58:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669069/SRX4669069.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。