Job ID = 6457588 SRX = SRX4669067 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:45:03 prefetch.2.10.7: 1) Downloading 'SRR7817592'... 2020-06-21T11:45:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:47:33 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:47:34 prefetch.2.10.7: 'SRR7817592' is valid 2020-06-21T11:47:34 prefetch.2.10.7: 1) 'SRR7817592' was downloaded successfully 2020-06-21T11:47:34 prefetch.2.10.7: 'SRR7817592' has 0 unresolved dependencies Read 25986033 spots for SRR7817592/SRR7817592.sra Written 25986033 spots for SRR7817592/SRR7817592.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:26 25986033 reads; of these: 25986033 (100.00%) were unpaired; of these: 2473531 (9.52%) aligned 0 times 21269684 (81.85%) aligned exactly 1 time 2242818 (8.63%) aligned >1 times 90.48% overall alignment rate Time searching: 00:05:26 Overall time: 00:05:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4637084 / 23512502 = 0.1972 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:58:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:58:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:58:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:58:50: 1000000 INFO @ Sun, 21 Jun 2020 20:58:56: 2000000 INFO @ Sun, 21 Jun 2020 20:59:01: 3000000 INFO @ Sun, 21 Jun 2020 20:59:06: 4000000 INFO @ Sun, 21 Jun 2020 20:59:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:59:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:59:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:59:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:59:17: 6000000 INFO @ Sun, 21 Jun 2020 20:59:21: 1000000 INFO @ Sun, 21 Jun 2020 20:59:23: 7000000 INFO @ Sun, 21 Jun 2020 20:59:27: 2000000 INFO @ Sun, 21 Jun 2020 20:59:28: 8000000 INFO @ Sun, 21 Jun 2020 20:59:33: 3000000 INFO @ Sun, 21 Jun 2020 20:59:34: 9000000 INFO @ Sun, 21 Jun 2020 20:59:39: 4000000 INFO @ Sun, 21 Jun 2020 20:59:40: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:59:45: 5000000 INFO @ Sun, 21 Jun 2020 20:59:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:59:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:59:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:59:46: 11000000 INFO @ Sun, 21 Jun 2020 20:59:51: 6000000 INFO @ Sun, 21 Jun 2020 20:59:51: 1000000 INFO @ Sun, 21 Jun 2020 20:59:52: 12000000 INFO @ Sun, 21 Jun 2020 20:59:57: 7000000 INFO @ Sun, 21 Jun 2020 20:59:57: 2000000 INFO @ Sun, 21 Jun 2020 20:59:58: 13000000 INFO @ Sun, 21 Jun 2020 21:00:03: 8000000 INFO @ Sun, 21 Jun 2020 21:00:03: 3000000 INFO @ Sun, 21 Jun 2020 21:00:04: 14000000 INFO @ Sun, 21 Jun 2020 21:00:08: 9000000 INFO @ Sun, 21 Jun 2020 21:00:09: 4000000 INFO @ Sun, 21 Jun 2020 21:00:10: 15000000 INFO @ Sun, 21 Jun 2020 21:00:14: 10000000 INFO @ Sun, 21 Jun 2020 21:00:15: 5000000 INFO @ Sun, 21 Jun 2020 21:00:16: 16000000 INFO @ Sun, 21 Jun 2020 21:00:20: 11000000 INFO @ Sun, 21 Jun 2020 21:00:21: 6000000 INFO @ Sun, 21 Jun 2020 21:00:22: 17000000 INFO @ Sun, 21 Jun 2020 21:00:26: 12000000 INFO @ Sun, 21 Jun 2020 21:00:27: 7000000 INFO @ Sun, 21 Jun 2020 21:00:28: 18000000 INFO @ Sun, 21 Jun 2020 21:00:32: 13000000 INFO @ Sun, 21 Jun 2020 21:00:33: 8000000 INFO @ Sun, 21 Jun 2020 21:00:33: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:00:33: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:00:33: #1 total tags in treatment: 18875418 INFO @ Sun, 21 Jun 2020 21:00:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:00:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:00:34: #1 tags after filtering in treatment: 18875389 INFO @ Sun, 21 Jun 2020 21:00:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:00:34: #1 finished! INFO @ Sun, 21 Jun 2020 21:00:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:00:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:00:35: #2 number of paired peaks: 58 WARNING @ Sun, 21 Jun 2020 21:00:35: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:00:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:00:38: 14000000 INFO @ Sun, 21 Jun 2020 21:00:39: 9000000 INFO @ Sun, 21 Jun 2020 21:00:44: 15000000 INFO @ Sun, 21 Jun 2020 21:00:45: 10000000 INFO @ Sun, 21 Jun 2020 21:00:50: 16000000 INFO @ Sun, 21 Jun 2020 21:00:50: 11000000 INFO @ Sun, 21 Jun 2020 21:00:56: 17000000 INFO @ Sun, 21 Jun 2020 21:00:56: 12000000 INFO @ Sun, 21 Jun 2020 21:01:02: 18000000 INFO @ Sun, 21 Jun 2020 21:01:02: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:01:07: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:01:07: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:01:07: #1 total tags in treatment: 18875418 INFO @ Sun, 21 Jun 2020 21:01:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:01:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:01:08: #1 tags after filtering in treatment: 18875389 INFO @ Sun, 21 Jun 2020 21:01:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:01:08: #1 finished! INFO @ Sun, 21 Jun 2020 21:01:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:01:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:01:08: 14000000 INFO @ Sun, 21 Jun 2020 21:01:09: #2 number of paired peaks: 58 WARNING @ Sun, 21 Jun 2020 21:01:09: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:01:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:01:14: 15000000 INFO @ Sun, 21 Jun 2020 21:01:19: 16000000 INFO @ Sun, 21 Jun 2020 21:01:25: 17000000 INFO @ Sun, 21 Jun 2020 21:01:30: 18000000 INFO @ Sun, 21 Jun 2020 21:01:35: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:01:35: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:01:35: #1 total tags in treatment: 18875418 INFO @ Sun, 21 Jun 2020 21:01:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:01:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:01:35: #1 tags after filtering in treatment: 18875389 INFO @ Sun, 21 Jun 2020 21:01:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:01:35: #1 finished! INFO @ Sun, 21 Jun 2020 21:01:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:01:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:01:36: #2 number of paired peaks: 58 WARNING @ Sun, 21 Jun 2020 21:01:36: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:01:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669067/SRX4669067.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。