Job ID = 6457586 SRX = SRX4669065 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:02:02 prefetch.2.10.7: 1) Downloading 'SRR7817590'... 2020-06-21T12:02:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:04:13 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:04:14 prefetch.2.10.7: 'SRR7817590' is valid 2020-06-21T12:04:14 prefetch.2.10.7: 1) 'SRR7817590' was downloaded successfully 2020-06-21T12:04:14 prefetch.2.10.7: 'SRR7817590' has 0 unresolved dependencies Read 28184477 spots for SRR7817590/SRR7817590.sra Written 28184477 spots for SRR7817590/SRR7817590.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:39 28184477 reads; of these: 28184477 (100.00%) were unpaired; of these: 1523049 (5.40%) aligned 0 times 23481484 (83.31%) aligned exactly 1 time 3179944 (11.28%) aligned >1 times 94.60% overall alignment rate Time searching: 00:06:39 Overall time: 00:06:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5281663 / 26661428 = 0.1981 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:17:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:17:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:17:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:17:40: 1000000 INFO @ Sun, 21 Jun 2020 21:17:47: 2000000 INFO @ Sun, 21 Jun 2020 21:17:54: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:18:01: 4000000 INFO @ Sun, 21 Jun 2020 21:18:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:18:03: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:18:03: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:18:08: 5000000 INFO @ Sun, 21 Jun 2020 21:18:10: 1000000 INFO @ Sun, 21 Jun 2020 21:18:15: 6000000 INFO @ Sun, 21 Jun 2020 21:18:17: 2000000 INFO @ Sun, 21 Jun 2020 21:18:23: 7000000 INFO @ Sun, 21 Jun 2020 21:18:25: 3000000 INFO @ Sun, 21 Jun 2020 21:18:30: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:18:32: 4000000 INFO @ Sun, 21 Jun 2020 21:18:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:18:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:18:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:18:37: 9000000 INFO @ Sun, 21 Jun 2020 21:18:39: 5000000 INFO @ Sun, 21 Jun 2020 21:18:40: 1000000 INFO @ Sun, 21 Jun 2020 21:18:45: 10000000 INFO @ Sun, 21 Jun 2020 21:18:46: 2000000 INFO @ Sun, 21 Jun 2020 21:18:47: 6000000 INFO @ Sun, 21 Jun 2020 21:18:52: 11000000 INFO @ Sun, 21 Jun 2020 21:18:52: 3000000 INFO @ Sun, 21 Jun 2020 21:18:54: 7000000 INFO @ Sun, 21 Jun 2020 21:18:59: 4000000 INFO @ Sun, 21 Jun 2020 21:18:59: 12000000 INFO @ Sun, 21 Jun 2020 21:19:01: 8000000 INFO @ Sun, 21 Jun 2020 21:19:05: 5000000 INFO @ Sun, 21 Jun 2020 21:19:07: 13000000 INFO @ Sun, 21 Jun 2020 21:19:09: 9000000 INFO @ Sun, 21 Jun 2020 21:19:12: 6000000 INFO @ Sun, 21 Jun 2020 21:19:14: 14000000 INFO @ Sun, 21 Jun 2020 21:19:16: 10000000 INFO @ Sun, 21 Jun 2020 21:19:18: 7000000 INFO @ Sun, 21 Jun 2020 21:19:21: 15000000 INFO @ Sun, 21 Jun 2020 21:19:23: 11000000 INFO @ Sun, 21 Jun 2020 21:19:24: 8000000 INFO @ Sun, 21 Jun 2020 21:19:28: 16000000 INFO @ Sun, 21 Jun 2020 21:19:30: 12000000 INFO @ Sun, 21 Jun 2020 21:19:31: 9000000 INFO @ Sun, 21 Jun 2020 21:19:36: 17000000 INFO @ Sun, 21 Jun 2020 21:19:37: 10000000 INFO @ Sun, 21 Jun 2020 21:19:38: 13000000 INFO @ Sun, 21 Jun 2020 21:19:43: 11000000 INFO @ Sun, 21 Jun 2020 21:19:44: 18000000 INFO @ Sun, 21 Jun 2020 21:19:45: 14000000 INFO @ Sun, 21 Jun 2020 21:19:50: 12000000 INFO @ Sun, 21 Jun 2020 21:19:51: 19000000 INFO @ Sun, 21 Jun 2020 21:19:52: 15000000 INFO @ Sun, 21 Jun 2020 21:19:56: 13000000 INFO @ Sun, 21 Jun 2020 21:19:59: 20000000 INFO @ Sun, 21 Jun 2020 21:19:59: 16000000 INFO @ Sun, 21 Jun 2020 21:20:03: 14000000 INFO @ Sun, 21 Jun 2020 21:20:06: 21000000 INFO @ Sun, 21 Jun 2020 21:20:07: 17000000 INFO @ Sun, 21 Jun 2020 21:20:09: 15000000 INFO @ Sun, 21 Jun 2020 21:20:09: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:20:09: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:20:09: #1 total tags in treatment: 21379765 INFO @ Sun, 21 Jun 2020 21:20:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:20:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:20:10: #1 tags after filtering in treatment: 21379757 INFO @ Sun, 21 Jun 2020 21:20:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:20:10: #1 finished! INFO @ Sun, 21 Jun 2020 21:20:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:20:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:20:12: #2 number of paired peaks: 76 WARNING @ Sun, 21 Jun 2020 21:20:12: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:20:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:20:15: 18000000 INFO @ Sun, 21 Jun 2020 21:20:16: 16000000 INFO @ Sun, 21 Jun 2020 21:20:22: 17000000 INFO @ Sun, 21 Jun 2020 21:20:23: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:20:29: 18000000 INFO @ Sun, 21 Jun 2020 21:20:31: 20000000 INFO @ Sun, 21 Jun 2020 21:20:36: 19000000 INFO @ Sun, 21 Jun 2020 21:20:39: 21000000 INFO @ Sun, 21 Jun 2020 21:20:42: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:20:42: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:20:42: #1 total tags in treatment: 21379765 INFO @ Sun, 21 Jun 2020 21:20:42: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:20:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:20:43: #1 tags after filtering in treatment: 21379757 INFO @ Sun, 21 Jun 2020 21:20:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:20:43: #1 finished! INFO @ Sun, 21 Jun 2020 21:20:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:20:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:20:43: 20000000 INFO @ Sun, 21 Jun 2020 21:20:44: #2 number of paired peaks: 76 WARNING @ Sun, 21 Jun 2020 21:20:44: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:20:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:20:49: 21000000 INFO @ Sun, 21 Jun 2020 21:20:52: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:20:52: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:20:52: #1 total tags in treatment: 21379765 INFO @ Sun, 21 Jun 2020 21:20:52: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:20:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:20:52: #1 tags after filtering in treatment: 21379757 INFO @ Sun, 21 Jun 2020 21:20:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:20:52: #1 finished! INFO @ Sun, 21 Jun 2020 21:20:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:20:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:20:54: #2 number of paired peaks: 76 WARNING @ Sun, 21 Jun 2020 21:20:54: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:20:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669065/SRX4669065.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。