Job ID = 6457584 SRX = SRX4669063 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:54:33 prefetch.2.10.7: 1) Downloading 'SRR7817588'... 2020-06-21T11:54:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:55:39 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:55:40 prefetch.2.10.7: 'SRR7817588' is valid 2020-06-21T11:55:40 prefetch.2.10.7: 1) 'SRR7817588' was downloaded successfully 2020-06-21T11:55:40 prefetch.2.10.7: 'SRR7817588' has 0 unresolved dependencies Read 10838247 spots for SRR7817588/SRR7817588.sra Written 10838247 spots for SRR7817588/SRR7817588.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:28 10838247 reads; of these: 10838247 (100.00%) were unpaired; of these: 736263 (6.79%) aligned 0 times 8828210 (81.45%) aligned exactly 1 time 1273774 (11.75%) aligned >1 times 93.21% overall alignment rate Time searching: 00:02:28 Overall time: 00:02:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1204889 / 10101984 = 0.1193 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:01:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:01:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:01:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:01:11: 1000000 INFO @ Sun, 21 Jun 2020 21:01:17: 2000000 INFO @ Sun, 21 Jun 2020 21:01:23: 3000000 INFO @ Sun, 21 Jun 2020 21:01:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:01:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:01:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:01:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:01:35: 5000000 INFO @ Sun, 21 Jun 2020 21:01:41: 1000000 INFO @ Sun, 21 Jun 2020 21:01:41: 6000000 INFO @ Sun, 21 Jun 2020 21:01:47: 2000000 INFO @ Sun, 21 Jun 2020 21:01:47: 7000000 INFO @ Sun, 21 Jun 2020 21:01:53: 3000000 INFO @ Sun, 21 Jun 2020 21:01:54: 8000000 INFO @ Sun, 21 Jun 2020 21:01:59: 4000000 INFO @ Sun, 21 Jun 2020 21:02:00: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:02:00: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:02:00: #1 total tags in treatment: 8897095 INFO @ Sun, 21 Jun 2020 21:02:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:02:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:02:00: #1 tags after filtering in treatment: 8897024 INFO @ Sun, 21 Jun 2020 21:02:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:02:00: #1 finished! INFO @ Sun, 21 Jun 2020 21:02:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:02:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:02:01: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 21:02:01: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:02:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:02:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:02:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:02:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:02:05: 5000000 INFO @ Sun, 21 Jun 2020 21:02:11: 1000000 INFO @ Sun, 21 Jun 2020 21:02:11: 6000000 INFO @ Sun, 21 Jun 2020 21:02:17: 7000000 INFO @ Sun, 21 Jun 2020 21:02:17: 2000000 INFO @ Sun, 21 Jun 2020 21:02:23: 3000000 INFO @ Sun, 21 Jun 2020 21:02:23: 8000000 INFO @ Sun, 21 Jun 2020 21:02:29: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:02:29: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:02:29: #1 total tags in treatment: 8897095 INFO @ Sun, 21 Jun 2020 21:02:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:02:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:02:29: 4000000 INFO @ Sun, 21 Jun 2020 21:02:30: #1 tags after filtering in treatment: 8897024 INFO @ Sun, 21 Jun 2020 21:02:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:02:30: #1 finished! INFO @ Sun, 21 Jun 2020 21:02:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:02:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:02:30: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 21:02:30: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:02:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:02:35: 5000000 INFO @ Sun, 21 Jun 2020 21:02:41: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:02:47: 7000000 INFO @ Sun, 21 Jun 2020 21:02:53: 8000000 INFO @ Sun, 21 Jun 2020 21:02:58: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:02:58: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:02:58: #1 total tags in treatment: 8897095 INFO @ Sun, 21 Jun 2020 21:02:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:02:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:02:58: #1 tags after filtering in treatment: 8897024 INFO @ Sun, 21 Jun 2020 21:02:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:02:58: #1 finished! INFO @ Sun, 21 Jun 2020 21:02:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:02:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:02:59: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 21:02:59: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:02:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669063/SRX4669063.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。