Job ID = 6457576 SRX = SRX4669060 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:55:03 prefetch.2.10.7: 1) Downloading 'SRR7817585'... 2020-06-21T11:55:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:57:43 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:57:44 prefetch.2.10.7: 'SRR7817585' is valid 2020-06-21T11:57:44 prefetch.2.10.7: 1) 'SRR7817585' was downloaded successfully 2020-06-21T11:57:44 prefetch.2.10.7: 'SRR7817585' has 0 unresolved dependencies Read 29516034 spots for SRR7817585/SRR7817585.sra Written 29516034 spots for SRR7817585/SRR7817585.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:53 29516034 reads; of these: 29516034 (100.00%) were unpaired; of these: 2166303 (7.34%) aligned 0 times 23893690 (80.95%) aligned exactly 1 time 3456041 (11.71%) aligned >1 times 92.66% overall alignment rate Time searching: 00:06:53 Overall time: 00:06:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5394580 / 27349731 = 0.1972 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:11:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:11:12: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:11:12: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:11:18: 1000000 INFO @ Sun, 21 Jun 2020 21:11:24: 2000000 INFO @ Sun, 21 Jun 2020 21:11:29: 3000000 INFO @ Sun, 21 Jun 2020 21:11:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:11:41: 5000000 INFO @ Sun, 21 Jun 2020 21:11:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:11:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:11:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:11:48: 6000000 INFO @ Sun, 21 Jun 2020 21:11:49: 1000000 INFO @ Sun, 21 Jun 2020 21:11:54: 7000000 INFO @ Sun, 21 Jun 2020 21:11:56: 2000000 INFO @ Sun, 21 Jun 2020 21:12:01: 8000000 INFO @ Sun, 21 Jun 2020 21:12:02: 3000000 INFO @ Sun, 21 Jun 2020 21:12:08: 9000000 INFO @ Sun, 21 Jun 2020 21:12:09: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:12:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:12:12: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:12:12: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:12:15: 10000000 INFO @ Sun, 21 Jun 2020 21:12:17: 5000000 INFO @ Sun, 21 Jun 2020 21:12:21: 1000000 INFO @ Sun, 21 Jun 2020 21:12:23: 11000000 INFO @ Sun, 21 Jun 2020 21:12:25: 6000000 INFO @ Sun, 21 Jun 2020 21:12:30: 2000000 INFO @ Sun, 21 Jun 2020 21:12:31: 12000000 INFO @ Sun, 21 Jun 2020 21:12:33: 7000000 INFO @ Sun, 21 Jun 2020 21:12:38: 3000000 INFO @ Sun, 21 Jun 2020 21:12:39: 13000000 INFO @ Sun, 21 Jun 2020 21:12:40: 8000000 INFO @ Sun, 21 Jun 2020 21:12:47: 14000000 INFO @ Sun, 21 Jun 2020 21:12:47: 4000000 INFO @ Sun, 21 Jun 2020 21:12:48: 9000000 INFO @ Sun, 21 Jun 2020 21:12:55: 15000000 INFO @ Sun, 21 Jun 2020 21:12:56: 5000000 INFO @ Sun, 21 Jun 2020 21:12:56: 10000000 INFO @ Sun, 21 Jun 2020 21:13:02: 16000000 INFO @ Sun, 21 Jun 2020 21:13:04: 11000000 INFO @ Sun, 21 Jun 2020 21:13:05: 6000000 INFO @ Sun, 21 Jun 2020 21:13:10: 17000000 INFO @ Sun, 21 Jun 2020 21:13:12: 12000000 INFO @ Sun, 21 Jun 2020 21:13:14: 7000000 INFO @ Sun, 21 Jun 2020 21:13:19: 18000000 INFO @ Sun, 21 Jun 2020 21:13:20: 13000000 INFO @ Sun, 21 Jun 2020 21:13:23: 8000000 INFO @ Sun, 21 Jun 2020 21:13:27: 19000000 INFO @ Sun, 21 Jun 2020 21:13:28: 14000000 INFO @ Sun, 21 Jun 2020 21:13:31: 9000000 INFO @ Sun, 21 Jun 2020 21:13:35: 20000000 INFO @ Sun, 21 Jun 2020 21:13:36: 15000000 INFO @ Sun, 21 Jun 2020 21:13:40: 10000000 INFO @ Sun, 21 Jun 2020 21:13:42: 21000000 INFO @ Sun, 21 Jun 2020 21:13:44: 16000000 INFO @ Sun, 21 Jun 2020 21:13:49: 11000000 INFO @ Sun, 21 Jun 2020 21:13:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:13:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:13:51: #1 total tags in treatment: 21955151 INFO @ Sun, 21 Jun 2020 21:13:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:13:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:13:51: #1 tags after filtering in treatment: 21955135 INFO @ Sun, 21 Jun 2020 21:13:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:13:51: #1 finished! INFO @ Sun, 21 Jun 2020 21:13:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:13:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:13:51: 17000000 INFO @ Sun, 21 Jun 2020 21:13:53: #2 number of paired peaks: 72 WARNING @ Sun, 21 Jun 2020 21:13:53: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:13:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:13:58: 12000000 INFO @ Sun, 21 Jun 2020 21:13:59: 18000000 INFO @ Sun, 21 Jun 2020 21:14:06: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:14:07: 19000000 INFO @ Sun, 21 Jun 2020 21:14:14: 14000000 INFO @ Sun, 21 Jun 2020 21:14:15: 20000000 INFO @ Sun, 21 Jun 2020 21:14:23: 21000000 INFO @ Sun, 21 Jun 2020 21:14:23: 15000000 INFO @ Sun, 21 Jun 2020 21:14:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:14:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:14:30: #1 total tags in treatment: 21955151 INFO @ Sun, 21 Jun 2020 21:14:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:14:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:14:31: #1 tags after filtering in treatment: 21955135 INFO @ Sun, 21 Jun 2020 21:14:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:14:31: #1 finished! INFO @ Sun, 21 Jun 2020 21:14:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:14:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:14:31: 16000000 INFO @ Sun, 21 Jun 2020 21:14:33: #2 number of paired peaks: 72 WARNING @ Sun, 21 Jun 2020 21:14:33: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:14:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:14:39: 17000000 INFO @ Sun, 21 Jun 2020 21:14:48: 18000000 INFO @ Sun, 21 Jun 2020 21:14:55: 19000000 INFO @ Sun, 21 Jun 2020 21:15:03: 20000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:15:11: 21000000 INFO @ Sun, 21 Jun 2020 21:15:19: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:15:19: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:15:19: #1 total tags in treatment: 21955151 INFO @ Sun, 21 Jun 2020 21:15:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:15:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:15:19: #1 tags after filtering in treatment: 21955135 INFO @ Sun, 21 Jun 2020 21:15:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:15:19: #1 finished! INFO @ Sun, 21 Jun 2020 21:15:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:15:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:15:21: #2 number of paired peaks: 72 WARNING @ Sun, 21 Jun 2020 21:15:21: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:15:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4669060/SRX4669060.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling