Job ID = 6457252 SRX = SRX4580637 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:39:40 prefetch.2.10.7: 1) Downloading 'SRR7724142'... 2020-06-21T11:39:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:48:22 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:48:22 prefetch.2.10.7: 1) 'SRR7724142' was downloaded successfully Read 51831789 spots for SRR7724142/SRR7724142.sra Written 51831789 spots for SRR7724142/SRR7724142.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:59 51831789 reads; of these: 51831789 (100.00%) were unpaired; of these: 4503746 (8.69%) aligned 0 times 38373503 (74.03%) aligned exactly 1 time 8954540 (17.28%) aligned >1 times 91.31% overall alignment rate Time searching: 00:11:59 Overall time: 00:11:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 6741175 / 47328043 = 0.1424 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:13:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:13:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:13:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:13:56: 1000000 INFO @ Sun, 21 Jun 2020 21:14:01: 2000000 INFO @ Sun, 21 Jun 2020 21:14:06: 3000000 INFO @ Sun, 21 Jun 2020 21:14:11: 4000000 INFO @ Sun, 21 Jun 2020 21:14:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:14:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:14:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:14:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:14:21: 6000000 INFO @ Sun, 21 Jun 2020 21:14:26: 7000000 INFO @ Sun, 21 Jun 2020 21:14:27: 1000000 INFO @ Sun, 21 Jun 2020 21:14:32: 8000000 INFO @ Sun, 21 Jun 2020 21:14:32: 2000000 INFO @ Sun, 21 Jun 2020 21:14:37: 9000000 INFO @ Sun, 21 Jun 2020 21:14:38: 3000000 INFO @ Sun, 21 Jun 2020 21:14:42: 10000000 INFO @ Sun, 21 Jun 2020 21:14:43: 4000000 INFO @ Sun, 21 Jun 2020 21:14:47: 11000000 INFO @ Sun, 21 Jun 2020 21:14:49: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:14:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:14:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:14:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:14:52: 12000000 INFO @ Sun, 21 Jun 2020 21:14:55: 6000000 INFO @ Sun, 21 Jun 2020 21:14:57: 1000000 INFO @ Sun, 21 Jun 2020 21:14:57: 13000000 INFO @ Sun, 21 Jun 2020 21:15:00: 7000000 INFO @ Sun, 21 Jun 2020 21:15:02: 2000000 INFO @ Sun, 21 Jun 2020 21:15:02: 14000000 INFO @ Sun, 21 Jun 2020 21:15:06: 8000000 INFO @ Sun, 21 Jun 2020 21:15:07: 15000000 INFO @ Sun, 21 Jun 2020 21:15:08: 3000000 INFO @ Sun, 21 Jun 2020 21:15:12: 9000000 INFO @ Sun, 21 Jun 2020 21:15:13: 16000000 INFO @ Sun, 21 Jun 2020 21:15:14: 4000000 INFO @ Sun, 21 Jun 2020 21:15:18: 10000000 INFO @ Sun, 21 Jun 2020 21:15:18: 17000000 INFO @ Sun, 21 Jun 2020 21:15:19: 5000000 INFO @ Sun, 21 Jun 2020 21:15:23: 18000000 INFO @ Sun, 21 Jun 2020 21:15:24: 11000000 INFO @ Sun, 21 Jun 2020 21:15:25: 6000000 INFO @ Sun, 21 Jun 2020 21:15:28: 19000000 INFO @ Sun, 21 Jun 2020 21:15:29: 12000000 INFO @ Sun, 21 Jun 2020 21:15:31: 7000000 INFO @ Sun, 21 Jun 2020 21:15:33: 20000000 INFO @ Sun, 21 Jun 2020 21:15:35: 13000000 INFO @ Sun, 21 Jun 2020 21:15:38: 8000000 INFO @ Sun, 21 Jun 2020 21:15:38: 21000000 INFO @ Sun, 21 Jun 2020 21:15:41: 14000000 INFO @ Sun, 21 Jun 2020 21:15:44: 22000000 INFO @ Sun, 21 Jun 2020 21:15:44: 9000000 INFO @ Sun, 21 Jun 2020 21:15:47: 15000000 INFO @ Sun, 21 Jun 2020 21:15:49: 23000000 INFO @ Sun, 21 Jun 2020 21:15:50: 10000000 INFO @ Sun, 21 Jun 2020 21:15:52: 16000000 INFO @ Sun, 21 Jun 2020 21:15:54: 24000000 INFO @ Sun, 21 Jun 2020 21:15:56: 11000000 INFO @ Sun, 21 Jun 2020 21:15:58: 17000000 INFO @ Sun, 21 Jun 2020 21:15:59: 25000000 INFO @ Sun, 21 Jun 2020 21:16:01: 12000000 INFO @ Sun, 21 Jun 2020 21:16:04: 18000000 INFO @ Sun, 21 Jun 2020 21:16:04: 26000000 INFO @ Sun, 21 Jun 2020 21:16:07: 13000000 INFO @ Sun, 21 Jun 2020 21:16:09: 27000000 INFO @ Sun, 21 Jun 2020 21:16:10: 19000000 INFO @ Sun, 21 Jun 2020 21:16:13: 14000000 INFO @ Sun, 21 Jun 2020 21:16:15: 28000000 INFO @ Sun, 21 Jun 2020 21:16:16: 20000000 INFO @ Sun, 21 Jun 2020 21:16:19: 15000000 INFO @ Sun, 21 Jun 2020 21:16:20: 29000000 INFO @ Sun, 21 Jun 2020 21:16:21: 21000000 INFO @ Sun, 21 Jun 2020 21:16:25: 16000000 INFO @ Sun, 21 Jun 2020 21:16:25: 30000000 INFO @ Sun, 21 Jun 2020 21:16:27: 22000000 INFO @ Sun, 21 Jun 2020 21:16:30: 31000000 INFO @ Sun, 21 Jun 2020 21:16:31: 17000000 INFO @ Sun, 21 Jun 2020 21:16:33: 23000000 INFO @ Sun, 21 Jun 2020 21:16:35: 32000000 INFO @ Sun, 21 Jun 2020 21:16:36: 18000000 INFO @ Sun, 21 Jun 2020 21:16:38: 24000000 INFO @ Sun, 21 Jun 2020 21:16:41: 33000000 INFO @ Sun, 21 Jun 2020 21:16:42: 19000000 INFO @ Sun, 21 Jun 2020 21:16:44: 25000000 INFO @ Sun, 21 Jun 2020 21:16:46: 34000000 INFO @ Sun, 21 Jun 2020 21:16:48: 20000000 INFO @ Sun, 21 Jun 2020 21:16:50: 26000000 INFO @ Sun, 21 Jun 2020 21:16:51: 35000000 INFO @ Sun, 21 Jun 2020 21:16:54: 21000000 INFO @ Sun, 21 Jun 2020 21:16:56: 27000000 INFO @ Sun, 21 Jun 2020 21:16:56: 36000000 INFO @ Sun, 21 Jun 2020 21:16:59: 22000000 INFO @ Sun, 21 Jun 2020 21:17:01: 37000000 INFO @ Sun, 21 Jun 2020 21:17:02: 28000000 INFO @ Sun, 21 Jun 2020 21:17:05: 23000000 INFO @ Sun, 21 Jun 2020 21:17:07: 38000000 INFO @ Sun, 21 Jun 2020 21:17:07: 29000000 INFO @ Sun, 21 Jun 2020 21:17:11: 24000000 INFO @ Sun, 21 Jun 2020 21:17:12: 39000000 INFO @ Sun, 21 Jun 2020 21:17:13: 30000000 INFO @ Sun, 21 Jun 2020 21:17:17: 25000000 INFO @ Sun, 21 Jun 2020 21:17:17: 40000000 INFO @ Sun, 21 Jun 2020 21:17:19: 31000000 INFO @ Sun, 21 Jun 2020 21:17:20: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:17:20: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:17:20: #1 total tags in treatment: 40586868 INFO @ Sun, 21 Jun 2020 21:17:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:17:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:17:21: #1 tags after filtering in treatment: 40586802 INFO @ Sun, 21 Jun 2020 21:17:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:17:21: #1 finished! INFO @ Sun, 21 Jun 2020 21:17:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:17:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:17:22: 26000000 INFO @ Sun, 21 Jun 2020 21:17:24: #2 number of paired peaks: 90 WARNING @ Sun, 21 Jun 2020 21:17:24: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:17:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:17:24: 32000000 INFO @ Sun, 21 Jun 2020 21:17:28: 27000000 INFO @ Sun, 21 Jun 2020 21:17:30: 33000000 INFO @ Sun, 21 Jun 2020 21:17:34: 28000000 INFO @ Sun, 21 Jun 2020 21:17:36: 34000000 INFO @ Sun, 21 Jun 2020 21:17:40: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:17:42: 35000000 INFO @ Sun, 21 Jun 2020 21:17:45: 30000000 INFO @ Sun, 21 Jun 2020 21:17:48: 36000000 INFO @ Sun, 21 Jun 2020 21:17:51: 31000000 INFO @ Sun, 21 Jun 2020 21:17:53: 37000000 INFO @ Sun, 21 Jun 2020 21:17:57: 32000000 INFO @ Sun, 21 Jun 2020 21:17:59: 38000000 INFO @ Sun, 21 Jun 2020 21:18:03: 33000000 INFO @ Sun, 21 Jun 2020 21:18:05: 39000000 INFO @ Sun, 21 Jun 2020 21:18:08: 34000000 INFO @ Sun, 21 Jun 2020 21:18:10: 40000000 INFO @ Sun, 21 Jun 2020 21:18:14: 35000000 INFO @ Sun, 21 Jun 2020 21:18:14: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:18:14: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:18:14: #1 total tags in treatment: 40586868 INFO @ Sun, 21 Jun 2020 21:18:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:18:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:18:15: #1 tags after filtering in treatment: 40586802 INFO @ Sun, 21 Jun 2020 21:18:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:18:15: #1 finished! INFO @ Sun, 21 Jun 2020 21:18:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:18:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:18:18: #2 number of paired peaks: 90 WARNING @ Sun, 21 Jun 2020 21:18:18: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:18:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:18:20: 36000000 INFO @ Sun, 21 Jun 2020 21:18:25: 37000000 INFO @ Sun, 21 Jun 2020 21:18:31: 38000000 INFO @ Sun, 21 Jun 2020 21:18:37: 39000000 INFO @ Sun, 21 Jun 2020 21:18:42: 40000000 INFO @ Sun, 21 Jun 2020 21:18:46: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:18:46: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:18:46: #1 total tags in treatment: 40586868 INFO @ Sun, 21 Jun 2020 21:18:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:18:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:18:47: #1 tags after filtering in treatment: 40586802 INFO @ Sun, 21 Jun 2020 21:18:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:18:47: #1 finished! INFO @ Sun, 21 Jun 2020 21:18:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:18:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:18:49: #2 number of paired peaks: 90 WARNING @ Sun, 21 Jun 2020 21:18:49: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:18:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4580637/SRX4580637.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。