Job ID = 6457218 SRX = SRX457598 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:34:40 prefetch.2.10.7: 1) Downloading 'SRR1153274'... 2020-06-21T11:34:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:54:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:54:26 prefetch.2.10.7: 1) 'SRR1153274' was downloaded successfully Read 109944906 spots for SRR1153274/SRR1153274.sra Written 109944906 spots for SRR1153274/SRR1153274.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:32:39 109944906 reads; of these: 109944906 (100.00%) were unpaired; of these: 6019174 (5.47%) aligned 0 times 80885339 (73.57%) aligned exactly 1 time 23040393 (20.96%) aligned >1 times 94.53% overall alignment rate Time searching: 00:32:40 Overall time: 00:32:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 56237203 / 103925732 = 0.5411 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:57:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:57:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:57:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:57:07: 1000000 INFO @ Sun, 21 Jun 2020 21:57:12: 2000000 INFO @ Sun, 21 Jun 2020 21:57:18: 3000000 INFO @ Sun, 21 Jun 2020 21:57:23: 4000000 INFO @ Sun, 21 Jun 2020 21:57:29: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:57:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:57:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:57:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:57:35: 6000000 INFO @ Sun, 21 Jun 2020 21:57:37: 1000000 INFO @ Sun, 21 Jun 2020 21:57:41: 7000000 INFO @ Sun, 21 Jun 2020 21:57:43: 2000000 INFO @ Sun, 21 Jun 2020 21:57:47: 8000000 INFO @ Sun, 21 Jun 2020 21:57:49: 3000000 INFO @ Sun, 21 Jun 2020 21:57:54: 9000000 INFO @ Sun, 21 Jun 2020 21:57:55: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:58:00: 10000000 INFO @ Sun, 21 Jun 2020 21:58:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:58:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:58:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:58:01: 5000000 INFO @ Sun, 21 Jun 2020 21:58:06: 11000000 INFO @ Sun, 21 Jun 2020 21:58:08: 6000000 INFO @ Sun, 21 Jun 2020 21:58:08: 1000000 INFO @ Sun, 21 Jun 2020 21:58:13: 12000000 INFO @ Sun, 21 Jun 2020 21:58:14: 7000000 INFO @ Sun, 21 Jun 2020 21:58:15: 2000000 INFO @ Sun, 21 Jun 2020 21:58:19: 13000000 INFO @ Sun, 21 Jun 2020 21:58:20: 8000000 INFO @ Sun, 21 Jun 2020 21:58:22: 3000000 INFO @ Sun, 21 Jun 2020 21:58:26: 14000000 INFO @ Sun, 21 Jun 2020 21:58:27: 9000000 INFO @ Sun, 21 Jun 2020 21:58:29: 4000000 INFO @ Sun, 21 Jun 2020 21:58:32: 15000000 INFO @ Sun, 21 Jun 2020 21:58:33: 10000000 INFO @ Sun, 21 Jun 2020 21:58:36: 5000000 INFO @ Sun, 21 Jun 2020 21:58:39: 16000000 INFO @ Sun, 21 Jun 2020 21:58:39: 11000000 INFO @ Sun, 21 Jun 2020 21:58:43: 6000000 INFO @ Sun, 21 Jun 2020 21:58:45: 17000000 INFO @ Sun, 21 Jun 2020 21:58:46: 12000000 INFO @ Sun, 21 Jun 2020 21:58:50: 7000000 INFO @ Sun, 21 Jun 2020 21:58:52: 13000000 INFO @ Sun, 21 Jun 2020 21:58:52: 18000000 INFO @ Sun, 21 Jun 2020 21:58:57: 8000000 INFO @ Sun, 21 Jun 2020 21:58:58: 14000000 INFO @ Sun, 21 Jun 2020 21:58:59: 19000000 INFO @ Sun, 21 Jun 2020 21:59:05: 9000000 INFO @ Sun, 21 Jun 2020 21:59:05: 15000000 INFO @ Sun, 21 Jun 2020 21:59:05: 20000000 INFO @ Sun, 21 Jun 2020 21:59:11: 16000000 INFO @ Sun, 21 Jun 2020 21:59:11: 21000000 INFO @ Sun, 21 Jun 2020 21:59:12: 10000000 INFO @ Sun, 21 Jun 2020 21:59:17: 17000000 INFO @ Sun, 21 Jun 2020 21:59:18: 22000000 INFO @ Sun, 21 Jun 2020 21:59:19: 11000000 INFO @ Sun, 21 Jun 2020 21:59:24: 18000000 INFO @ Sun, 21 Jun 2020 21:59:25: 23000000 INFO @ Sun, 21 Jun 2020 21:59:26: 12000000 INFO @ Sun, 21 Jun 2020 21:59:30: 19000000 INFO @ Sun, 21 Jun 2020 21:59:31: 24000000 INFO @ Sun, 21 Jun 2020 21:59:33: 13000000 INFO @ Sun, 21 Jun 2020 21:59:37: 20000000 INFO @ Sun, 21 Jun 2020 21:59:37: 25000000 INFO @ Sun, 21 Jun 2020 21:59:39: 14000000 INFO @ Sun, 21 Jun 2020 21:59:43: 21000000 INFO @ Sun, 21 Jun 2020 21:59:44: 26000000 INFO @ Sun, 21 Jun 2020 21:59:46: 15000000 INFO @ Sun, 21 Jun 2020 21:59:49: 22000000 INFO @ Sun, 21 Jun 2020 21:59:51: 27000000 INFO @ Sun, 21 Jun 2020 21:59:52: 16000000 INFO @ Sun, 21 Jun 2020 21:59:56: 23000000 INFO @ Sun, 21 Jun 2020 21:59:57: 28000000 INFO @ Sun, 21 Jun 2020 21:59:59: 17000000 INFO @ Sun, 21 Jun 2020 22:00:02: 24000000 INFO @ Sun, 21 Jun 2020 22:00:04: 29000000 INFO @ Sun, 21 Jun 2020 22:00:05: 18000000 INFO @ Sun, 21 Jun 2020 22:00:09: 25000000 INFO @ Sun, 21 Jun 2020 22:00:10: 30000000 INFO @ Sun, 21 Jun 2020 22:00:12: 19000000 INFO @ Sun, 21 Jun 2020 22:00:15: 26000000 INFO @ Sun, 21 Jun 2020 22:00:16: 31000000 INFO @ Sun, 21 Jun 2020 22:00:19: 20000000 INFO @ Sun, 21 Jun 2020 22:00:21: 27000000 INFO @ Sun, 21 Jun 2020 22:00:23: 32000000 INFO @ Sun, 21 Jun 2020 22:00:26: 21000000 INFO @ Sun, 21 Jun 2020 22:00:28: 28000000 INFO @ Sun, 21 Jun 2020 22:00:29: 33000000 INFO @ Sun, 21 Jun 2020 22:00:33: 22000000 INFO @ Sun, 21 Jun 2020 22:00:34: 29000000 INFO @ Sun, 21 Jun 2020 22:00:35: 34000000 INFO @ Sun, 21 Jun 2020 22:00:40: 23000000 INFO @ Sun, 21 Jun 2020 22:00:41: 30000000 INFO @ Sun, 21 Jun 2020 22:00:42: 35000000 INFO @ Sun, 21 Jun 2020 22:00:46: 24000000 INFO @ Sun, 21 Jun 2020 22:00:47: 31000000 INFO @ Sun, 21 Jun 2020 22:00:48: 36000000 INFO @ Sun, 21 Jun 2020 22:00:53: 25000000 INFO @ Sun, 21 Jun 2020 22:00:53: 32000000 INFO @ Sun, 21 Jun 2020 22:00:54: 37000000 INFO @ Sun, 21 Jun 2020 22:00:59: 26000000 INFO @ Sun, 21 Jun 2020 22:01:00: 33000000 INFO @ Sun, 21 Jun 2020 22:01:01: 38000000 INFO @ Sun, 21 Jun 2020 22:01:06: 27000000 INFO @ Sun, 21 Jun 2020 22:01:06: 34000000 INFO @ Sun, 21 Jun 2020 22:01:07: 39000000 INFO @ Sun, 21 Jun 2020 22:01:12: 28000000 INFO @ Sun, 21 Jun 2020 22:01:12: 35000000 INFO @ Sun, 21 Jun 2020 22:01:13: 40000000 INFO @ Sun, 21 Jun 2020 22:01:18: 29000000 INFO @ Sun, 21 Jun 2020 22:01:19: 36000000 INFO @ Sun, 21 Jun 2020 22:01:20: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:01:25: 30000000 INFO @ Sun, 21 Jun 2020 22:01:25: 37000000 INFO @ Sun, 21 Jun 2020 22:01:26: 42000000 INFO @ Sun, 21 Jun 2020 22:01:31: 31000000 INFO @ Sun, 21 Jun 2020 22:01:32: 38000000 INFO @ Sun, 21 Jun 2020 22:01:32: 43000000 INFO @ Sun, 21 Jun 2020 22:01:37: 32000000 INFO @ Sun, 21 Jun 2020 22:01:38: 39000000 INFO @ Sun, 21 Jun 2020 22:01:39: 44000000 INFO @ Sun, 21 Jun 2020 22:01:44: 33000000 INFO @ Sun, 21 Jun 2020 22:01:45: 40000000 INFO @ Sun, 21 Jun 2020 22:01:45: 45000000 INFO @ Sun, 21 Jun 2020 22:01:50: 34000000 INFO @ Sun, 21 Jun 2020 22:01:51: 41000000 INFO @ Sun, 21 Jun 2020 22:01:51: 46000000 INFO @ Sun, 21 Jun 2020 22:01:56: 35000000 INFO @ Sun, 21 Jun 2020 22:01:57: 42000000 INFO @ Sun, 21 Jun 2020 22:01:58: 47000000 INFO @ Sun, 21 Jun 2020 22:02:02: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:02:02: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:02:02: #1 total tags in treatment: 47688529 INFO @ Sun, 21 Jun 2020 22:02:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:02:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:02:03: 36000000 INFO @ Sun, 21 Jun 2020 22:02:03: #1 tags after filtering in treatment: 47688529 INFO @ Sun, 21 Jun 2020 22:02:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:02:03: #1 finished! INFO @ Sun, 21 Jun 2020 22:02:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:02:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:02:03: 43000000 INFO @ Sun, 21 Jun 2020 22:02:06: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 22:02:06: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:02:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:02:09: 37000000 INFO @ Sun, 21 Jun 2020 22:02:10: 44000000 INFO @ Sun, 21 Jun 2020 22:02:15: 38000000 INFO @ Sun, 21 Jun 2020 22:02:16: 45000000 INFO @ Sun, 21 Jun 2020 22:02:22: 39000000 INFO @ Sun, 21 Jun 2020 22:02:22: 46000000 INFO @ Sun, 21 Jun 2020 22:02:28: 40000000 INFO @ Sun, 21 Jun 2020 22:02:28: 47000000 INFO @ Sun, 21 Jun 2020 22:02:33: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:02:33: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:02:33: #1 total tags in treatment: 47688529 INFO @ Sun, 21 Jun 2020 22:02:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:02:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:02:34: #1 tags after filtering in treatment: 47688529 INFO @ Sun, 21 Jun 2020 22:02:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:02:34: #1 finished! INFO @ Sun, 21 Jun 2020 22:02:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:02:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:02:34: 41000000 INFO @ Sun, 21 Jun 2020 22:02:37: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 22:02:37: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:02:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:02:40: 42000000 INFO @ Sun, 21 Jun 2020 22:02:46: 43000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:02:52: 44000000 INFO @ Sun, 21 Jun 2020 22:02:58: 45000000 INFO @ Sun, 21 Jun 2020 22:03:04: 46000000 INFO @ Sun, 21 Jun 2020 22:03:09: 47000000 INFO @ Sun, 21 Jun 2020 22:03:14: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:03:14: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:03:14: #1 total tags in treatment: 47688529 INFO @ Sun, 21 Jun 2020 22:03:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:03:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:03:15: #1 tags after filtering in treatment: 47688529 INFO @ Sun, 21 Jun 2020 22:03:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:03:15: #1 finished! INFO @ Sun, 21 Jun 2020 22:03:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:03:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:03:18: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 22:03:18: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:03:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX457598/SRX457598.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling