Job ID = 6457157 SRX = SRX450792 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:30:25 prefetch.2.10.7: 1) Downloading 'SRR1145609'... 2020-06-21T11:30:25 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:32:58 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:32:58 prefetch.2.10.7: 1) 'SRR1145609' was downloaded successfully Read 36813441 spots for SRR1145609/SRR1145609.sra Written 36813441 spots for SRR1145609/SRR1145609.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:00 36813441 reads; of these: 36813441 (100.00%) were unpaired; of these: 16933143 (46.00%) aligned 0 times 18097078 (49.16%) aligned exactly 1 time 1783220 (4.84%) aligned >1 times 54.00% overall alignment rate Time searching: 00:05:00 Overall time: 00:05:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6317147 / 19880298 = 0.3178 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:43:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:43:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:43:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:43:50: 1000000 INFO @ Sun, 21 Jun 2020 20:43:57: 2000000 INFO @ Sun, 21 Jun 2020 20:44:03: 3000000 INFO @ Sun, 21 Jun 2020 20:44:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:44:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:44:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:44:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:44:17: 5000000 INFO @ Sun, 21 Jun 2020 20:44:21: 1000000 INFO @ Sun, 21 Jun 2020 20:44:24: 6000000 INFO @ Sun, 21 Jun 2020 20:44:28: 2000000 INFO @ Sun, 21 Jun 2020 20:44:31: 7000000 INFO @ Sun, 21 Jun 2020 20:44:35: 3000000 INFO @ Sun, 21 Jun 2020 20:44:39: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:44:43: 4000000 INFO @ Sun, 21 Jun 2020 20:44:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:44:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:44:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:44:46: 9000000 INFO @ Sun, 21 Jun 2020 20:44:50: 5000000 INFO @ Sun, 21 Jun 2020 20:44:51: 1000000 INFO @ Sun, 21 Jun 2020 20:44:53: 10000000 INFO @ Sun, 21 Jun 2020 20:44:57: 6000000 INFO @ Sun, 21 Jun 2020 20:44:59: 2000000 INFO @ Sun, 21 Jun 2020 20:45:00: 11000000 INFO @ Sun, 21 Jun 2020 20:45:05: 7000000 INFO @ Sun, 21 Jun 2020 20:45:06: 3000000 INFO @ Sun, 21 Jun 2020 20:45:08: 12000000 INFO @ Sun, 21 Jun 2020 20:45:12: 8000000 INFO @ Sun, 21 Jun 2020 20:45:14: 4000000 INFO @ Sun, 21 Jun 2020 20:45:15: 13000000 INFO @ Sun, 21 Jun 2020 20:45:19: #1 tag size is determined as 41 bps INFO @ Sun, 21 Jun 2020 20:45:19: #1 tag size = 41 INFO @ Sun, 21 Jun 2020 20:45:19: #1 total tags in treatment: 13563151 INFO @ Sun, 21 Jun 2020 20:45:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:45:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:45:19: #1 tags after filtering in treatment: 13563002 INFO @ Sun, 21 Jun 2020 20:45:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:45:19: #1 finished! INFO @ Sun, 21 Jun 2020 20:45:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:45:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:45:20: 9000000 INFO @ Sun, 21 Jun 2020 20:45:20: #2 number of paired peaks: 60 WARNING @ Sun, 21 Jun 2020 20:45:20: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:45:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:45:21: 5000000 INFO @ Sun, 21 Jun 2020 20:45:27: 10000000 INFO @ Sun, 21 Jun 2020 20:45:28: 6000000 INFO @ Sun, 21 Jun 2020 20:45:34: 11000000 INFO @ Sun, 21 Jun 2020 20:45:35: 7000000 INFO @ Sun, 21 Jun 2020 20:45:42: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:45:43: 8000000 INFO @ Sun, 21 Jun 2020 20:45:49: 13000000 INFO @ Sun, 21 Jun 2020 20:45:50: 9000000 INFO @ Sun, 21 Jun 2020 20:45:53: #1 tag size is determined as 41 bps INFO @ Sun, 21 Jun 2020 20:45:53: #1 tag size = 41 INFO @ Sun, 21 Jun 2020 20:45:53: #1 total tags in treatment: 13563151 INFO @ Sun, 21 Jun 2020 20:45:53: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:45:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:45:54: #1 tags after filtering in treatment: 13563002 INFO @ Sun, 21 Jun 2020 20:45:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:45:54: #1 finished! INFO @ Sun, 21 Jun 2020 20:45:54: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:45:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:45:55: #2 number of paired peaks: 60 WARNING @ Sun, 21 Jun 2020 20:45:55: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:45:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:45:57: 10000000 INFO @ Sun, 21 Jun 2020 20:46:03: 11000000 INFO @ Sun, 21 Jun 2020 20:46:10: 12000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:46:17: 13000000 INFO @ Sun, 21 Jun 2020 20:46:21: #1 tag size is determined as 41 bps INFO @ Sun, 21 Jun 2020 20:46:21: #1 tag size = 41 INFO @ Sun, 21 Jun 2020 20:46:21: #1 total tags in treatment: 13563151 INFO @ Sun, 21 Jun 2020 20:46:21: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:46:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:46:21: #1 tags after filtering in treatment: 13563002 INFO @ Sun, 21 Jun 2020 20:46:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:46:21: #1 finished! INFO @ Sun, 21 Jun 2020 20:46:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:46:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:46:22: #2 number of paired peaks: 60 WARNING @ Sun, 21 Jun 2020 20:46:22: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:46:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450792/SRX450792.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling