Job ID = 6457156 SRX = SRX450791 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:32:55 prefetch.2.10.7: 1) Downloading 'SRR1145608'... 2020-06-21T11:32:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:38:09 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:38:09 prefetch.2.10.7: 1) 'SRR1145608' was downloaded successfully Read 35703091 spots for SRR1145608/SRR1145608.sra Written 35703091 spots for SRR1145608/SRR1145608.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:24 35703091 reads; of these: 35703091 (100.00%) were unpaired; of these: 27808933 (77.89%) aligned 0 times 7175771 (20.10%) aligned exactly 1 time 718387 (2.01%) aligned >1 times 22.11% overall alignment rate Time searching: 00:03:25 Overall time: 00:03:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1307117 / 7894158 = 0.1656 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:45:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:45:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:45:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:45:27: 1000000 INFO @ Sun, 21 Jun 2020 20:45:32: 2000000 INFO @ Sun, 21 Jun 2020 20:45:38: 3000000 INFO @ Sun, 21 Jun 2020 20:45:43: 4000000 INFO @ Sun, 21 Jun 2020 20:45:48: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:45:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:45:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:45:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:45:55: 6000000 INFO @ Sun, 21 Jun 2020 20:45:59: 1000000 INFO @ Sun, 21 Jun 2020 20:45:59: #1 tag size is determined as 41 bps INFO @ Sun, 21 Jun 2020 20:45:59: #1 tag size = 41 INFO @ Sun, 21 Jun 2020 20:45:59: #1 total tags in treatment: 6587041 INFO @ Sun, 21 Jun 2020 20:45:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:45:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:46:00: #1 tags after filtering in treatment: 6586779 INFO @ Sun, 21 Jun 2020 20:46:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:46:00: #1 finished! INFO @ Sun, 21 Jun 2020 20:46:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:46:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:46:00: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 20:46:00: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:46:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:46:06: 2000000 INFO @ Sun, 21 Jun 2020 20:46:13: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:46:20: 4000000 INFO @ Sun, 21 Jun 2020 20:46:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:46:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:46:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:46:28: 5000000 INFO @ Sun, 21 Jun 2020 20:46:28: 1000000 INFO @ Sun, 21 Jun 2020 20:46:35: 2000000 INFO @ Sun, 21 Jun 2020 20:46:36: 6000000 INFO @ Sun, 21 Jun 2020 20:46:40: #1 tag size is determined as 41 bps INFO @ Sun, 21 Jun 2020 20:46:40: #1 tag size = 41 INFO @ Sun, 21 Jun 2020 20:46:40: #1 total tags in treatment: 6587041 INFO @ Sun, 21 Jun 2020 20:46:40: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:46:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:46:40: #1 tags after filtering in treatment: 6586779 INFO @ Sun, 21 Jun 2020 20:46:40: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:46:40: #1 finished! INFO @ Sun, 21 Jun 2020 20:46:40: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:46:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:46:41: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 20:46:41: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:46:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:46:42: 3000000 INFO @ Sun, 21 Jun 2020 20:46:48: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:46:54: 5000000 INFO @ Sun, 21 Jun 2020 20:47:00: 6000000 INFO @ Sun, 21 Jun 2020 20:47:03: #1 tag size is determined as 41 bps INFO @ Sun, 21 Jun 2020 20:47:03: #1 tag size = 41 INFO @ Sun, 21 Jun 2020 20:47:03: #1 total tags in treatment: 6587041 INFO @ Sun, 21 Jun 2020 20:47:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:47:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:47:04: #1 tags after filtering in treatment: 6586779 INFO @ Sun, 21 Jun 2020 20:47:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:47:04: #1 finished! INFO @ Sun, 21 Jun 2020 20:47:04: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:47:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:47:04: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 20:47:04: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:47:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX450791/SRX450791.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。