Job ID = 6456967 SRX = SRX4158199 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:44:06 prefetch.2.10.7: 1) Downloading 'SRR7253479'... 2020-06-21T11:44:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:47:32 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:47:32 prefetch.2.10.7: 1) 'SRR7253479' was downloaded successfully Read 27087679 spots for SRR7253479/SRR7253479.sra Written 27087679 spots for SRR7253479/SRR7253479.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:22 27087679 reads; of these: 27087679 (100.00%) were unpaired; of these: 1107033 (4.09%) aligned 0 times 22758296 (84.02%) aligned exactly 1 time 3222350 (11.90%) aligned >1 times 95.91% overall alignment rate Time searching: 00:06:22 Overall time: 00:06:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2746243 / 25980646 = 0.1057 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:03:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:03:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:03:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:03:33: 1000000 INFO @ Sun, 21 Jun 2020 21:03:41: 2000000 INFO @ Sun, 21 Jun 2020 21:03:50: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:03:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:03:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:03:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:03:59: 4000000 INFO @ Sun, 21 Jun 2020 21:04:03: 1000000 INFO @ Sun, 21 Jun 2020 21:04:07: 5000000 INFO @ Sun, 21 Jun 2020 21:04:13: 2000000 INFO @ Sun, 21 Jun 2020 21:04:16: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:04:22: 3000000 INFO @ Sun, 21 Jun 2020 21:04:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:04:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:04:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:04:24: 7000000 INFO @ Sun, 21 Jun 2020 21:04:32: 4000000 INFO @ Sun, 21 Jun 2020 21:04:32: 1000000 INFO @ Sun, 21 Jun 2020 21:04:33: 8000000 INFO @ Sun, 21 Jun 2020 21:04:41: 2000000 INFO @ Sun, 21 Jun 2020 21:04:41: 9000000 INFO @ Sun, 21 Jun 2020 21:04:42: 5000000 INFO @ Sun, 21 Jun 2020 21:04:49: 3000000 INFO @ Sun, 21 Jun 2020 21:04:50: 10000000 INFO @ Sun, 21 Jun 2020 21:04:51: 6000000 INFO @ Sun, 21 Jun 2020 21:04:58: 4000000 INFO @ Sun, 21 Jun 2020 21:04:59: 11000000 INFO @ Sun, 21 Jun 2020 21:05:01: 7000000 INFO @ Sun, 21 Jun 2020 21:05:06: 5000000 INFO @ Sun, 21 Jun 2020 21:05:08: 12000000 INFO @ Sun, 21 Jun 2020 21:05:10: 8000000 INFO @ Sun, 21 Jun 2020 21:05:15: 6000000 INFO @ Sun, 21 Jun 2020 21:05:16: 13000000 INFO @ Sun, 21 Jun 2020 21:05:20: 9000000 INFO @ Sun, 21 Jun 2020 21:05:24: 7000000 INFO @ Sun, 21 Jun 2020 21:05:26: 14000000 INFO @ Sun, 21 Jun 2020 21:05:30: 10000000 INFO @ Sun, 21 Jun 2020 21:05:33: 8000000 INFO @ Sun, 21 Jun 2020 21:05:35: 15000000 INFO @ Sun, 21 Jun 2020 21:05:40: 11000000 INFO @ Sun, 21 Jun 2020 21:05:42: 9000000 INFO @ Sun, 21 Jun 2020 21:05:44: 16000000 INFO @ Sun, 21 Jun 2020 21:05:49: 12000000 INFO @ Sun, 21 Jun 2020 21:05:52: 10000000 INFO @ Sun, 21 Jun 2020 21:05:53: 17000000 INFO @ Sun, 21 Jun 2020 21:05:59: 13000000 INFO @ Sun, 21 Jun 2020 21:06:01: 11000000 INFO @ Sun, 21 Jun 2020 21:06:02: 18000000 INFO @ Sun, 21 Jun 2020 21:06:09: 14000000 INFO @ Sun, 21 Jun 2020 21:06:09: 12000000 INFO @ Sun, 21 Jun 2020 21:06:11: 19000000 INFO @ Sun, 21 Jun 2020 21:06:18: 13000000 INFO @ Sun, 21 Jun 2020 21:06:19: 15000000 INFO @ Sun, 21 Jun 2020 21:06:20: 20000000 INFO @ Sun, 21 Jun 2020 21:06:27: 14000000 INFO @ Sun, 21 Jun 2020 21:06:29: 16000000 INFO @ Sun, 21 Jun 2020 21:06:29: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:06:36: 15000000 INFO @ Sun, 21 Jun 2020 21:06:38: 22000000 INFO @ Sun, 21 Jun 2020 21:06:38: 17000000 INFO @ Sun, 21 Jun 2020 21:06:44: 16000000 INFO @ Sun, 21 Jun 2020 21:06:46: 23000000 INFO @ Sun, 21 Jun 2020 21:06:48: 18000000 INFO @ Sun, 21 Jun 2020 21:06:48: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:06:48: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:06:48: #1 total tags in treatment: 23234403 INFO @ Sun, 21 Jun 2020 21:06:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:06:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:06:49: #1 tags after filtering in treatment: 23234319 INFO @ Sun, 21 Jun 2020 21:06:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:06:49: #1 finished! INFO @ Sun, 21 Jun 2020 21:06:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:06:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:06:51: #2 number of paired peaks: 73 WARNING @ Sun, 21 Jun 2020 21:06:51: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:06:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:06:52: 17000000 INFO @ Sun, 21 Jun 2020 21:06:58: 19000000 INFO @ Sun, 21 Jun 2020 21:07:01: 18000000 INFO @ Sun, 21 Jun 2020 21:07:07: 20000000 INFO @ Sun, 21 Jun 2020 21:07:09: 19000000 INFO @ Sun, 21 Jun 2020 21:07:17: 21000000 INFO @ Sun, 21 Jun 2020 21:07:18: 20000000 INFO @ Sun, 21 Jun 2020 21:07:26: 21000000 INFO @ Sun, 21 Jun 2020 21:07:26: 22000000 INFO @ Sun, 21 Jun 2020 21:07:33: 22000000 INFO @ Sun, 21 Jun 2020 21:07:35: 23000000 INFO @ Sun, 21 Jun 2020 21:07:38: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:07:38: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:07:38: #1 total tags in treatment: 23234403 INFO @ Sun, 21 Jun 2020 21:07:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:07:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:07:39: #1 tags after filtering in treatment: 23234319 INFO @ Sun, 21 Jun 2020 21:07:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:07:39: #1 finished! INFO @ Sun, 21 Jun 2020 21:07:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:07:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:07:40: #2 number of paired peaks: 73 WARNING @ Sun, 21 Jun 2020 21:07:40: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:07:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:07:41: 23000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:07:43: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:07:43: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:07:43: #1 total tags in treatment: 23234403 INFO @ Sun, 21 Jun 2020 21:07:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:07:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:07:44: #1 tags after filtering in treatment: 23234319 INFO @ Sun, 21 Jun 2020 21:07:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:07:44: #1 finished! INFO @ Sun, 21 Jun 2020 21:07:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:07:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:07:46: #2 number of paired peaks: 73 WARNING @ Sun, 21 Jun 2020 21:07:46: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:07:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158199/SRX4158199.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling