Job ID = 6456965 SRX = SRX4158197 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:19:12 prefetch.2.10.7: 1) Downloading 'SRR7253477'... 2020-06-21T11:19:12 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:23:52 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:23:52 prefetch.2.10.7: 1) 'SRR7253477' was downloaded successfully Read 32671823 spots for SRR7253477/SRR7253477.sra Written 32671823 spots for SRR7253477/SRR7253477.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:32 32671823 reads; of these: 32671823 (100.00%) were unpaired; of these: 1228063 (3.76%) aligned 0 times 28106595 (86.03%) aligned exactly 1 time 3337165 (10.21%) aligned >1 times 96.24% overall alignment rate Time searching: 00:08:33 Overall time: 00:08:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9586027 / 31443760 = 0.3049 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:41:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:41:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:41:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:41:35: 1000000 INFO @ Sun, 21 Jun 2020 20:41:41: 2000000 INFO @ Sun, 21 Jun 2020 20:41:46: 3000000 INFO @ Sun, 21 Jun 2020 20:41:52: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:41:58: 5000000 INFO @ Sun, 21 Jun 2020 20:42:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:42:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:42:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:42:04: 6000000 INFO @ Sun, 21 Jun 2020 20:42:06: 1000000 INFO @ Sun, 21 Jun 2020 20:42:10: 7000000 INFO @ Sun, 21 Jun 2020 20:42:12: 2000000 INFO @ Sun, 21 Jun 2020 20:42:15: 8000000 INFO @ Sun, 21 Jun 2020 20:42:18: 3000000 INFO @ Sun, 21 Jun 2020 20:42:21: 9000000 INFO @ Sun, 21 Jun 2020 20:42:24: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:42:27: 10000000 INFO @ Sun, 21 Jun 2020 20:42:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:42:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:42:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:42:30: 5000000 INFO @ Sun, 21 Jun 2020 20:42:33: 11000000 INFO @ Sun, 21 Jun 2020 20:42:35: 1000000 INFO @ Sun, 21 Jun 2020 20:42:36: 6000000 INFO @ Sun, 21 Jun 2020 20:42:39: 12000000 INFO @ Sun, 21 Jun 2020 20:42:41: 2000000 INFO @ Sun, 21 Jun 2020 20:42:42: 7000000 INFO @ Sun, 21 Jun 2020 20:42:45: 13000000 INFO @ Sun, 21 Jun 2020 20:42:47: 3000000 INFO @ Sun, 21 Jun 2020 20:42:48: 8000000 INFO @ Sun, 21 Jun 2020 20:42:51: 14000000 INFO @ Sun, 21 Jun 2020 20:42:53: 4000000 INFO @ Sun, 21 Jun 2020 20:42:54: 9000000 INFO @ Sun, 21 Jun 2020 20:42:57: 15000000 INFO @ Sun, 21 Jun 2020 20:42:59: 5000000 INFO @ Sun, 21 Jun 2020 20:43:00: 10000000 INFO @ Sun, 21 Jun 2020 20:43:03: 16000000 INFO @ Sun, 21 Jun 2020 20:43:05: 6000000 INFO @ Sun, 21 Jun 2020 20:43:06: 11000000 INFO @ Sun, 21 Jun 2020 20:43:09: 17000000 INFO @ Sun, 21 Jun 2020 20:43:12: 7000000 INFO @ Sun, 21 Jun 2020 20:43:12: 12000000 INFO @ Sun, 21 Jun 2020 20:43:16: 18000000 INFO @ Sun, 21 Jun 2020 20:43:18: 8000000 INFO @ Sun, 21 Jun 2020 20:43:18: 13000000 INFO @ Sun, 21 Jun 2020 20:43:22: 19000000 INFO @ Sun, 21 Jun 2020 20:43:24: 9000000 INFO @ Sun, 21 Jun 2020 20:43:24: 14000000 INFO @ Sun, 21 Jun 2020 20:43:28: 20000000 INFO @ Sun, 21 Jun 2020 20:43:29: 10000000 INFO @ Sun, 21 Jun 2020 20:43:31: 15000000 INFO @ Sun, 21 Jun 2020 20:43:34: 21000000 INFO @ Sun, 21 Jun 2020 20:43:35: 11000000 INFO @ Sun, 21 Jun 2020 20:43:37: 16000000 INFO @ Sun, 21 Jun 2020 20:43:39: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:43:39: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:43:39: #1 total tags in treatment: 21857733 INFO @ Sun, 21 Jun 2020 20:43:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:43:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:43:40: #1 tags after filtering in treatment: 21857635 INFO @ Sun, 21 Jun 2020 20:43:40: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:43:40: #1 finished! INFO @ Sun, 21 Jun 2020 20:43:40: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:43:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:43:41: #2 number of paired peaks: 74 WARNING @ Sun, 21 Jun 2020 20:43:41: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:43:41: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 20:43:41: 12000000 cut: /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:43:43: 17000000 INFO @ Sun, 21 Jun 2020 20:43:47: 13000000 INFO @ Sun, 21 Jun 2020 20:43:49: 18000000 INFO @ Sun, 21 Jun 2020 20:43:53: 14000000 INFO @ Sun, 21 Jun 2020 20:43:55: 19000000 INFO @ Sun, 21 Jun 2020 20:43:59: 15000000 INFO @ Sun, 21 Jun 2020 20:44:01: 20000000 INFO @ Sun, 21 Jun 2020 20:44:05: 16000000 INFO @ Sun, 21 Jun 2020 20:44:07: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:44:11: 17000000 INFO @ Sun, 21 Jun 2020 20:44:13: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:44:13: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:44:13: #1 total tags in treatment: 21857733 INFO @ Sun, 21 Jun 2020 20:44:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:44:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:44:13: #1 tags after filtering in treatment: 21857635 INFO @ Sun, 21 Jun 2020 20:44:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:44:13: #1 finished! INFO @ Sun, 21 Jun 2020 20:44:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:44:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:44:15: #2 number of paired peaks: 74 WARNING @ Sun, 21 Jun 2020 20:44:15: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:44:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:44:17: 18000000 INFO @ Sun, 21 Jun 2020 20:44:23: 19000000 INFO @ Sun, 21 Jun 2020 20:44:28: 20000000 INFO @ Sun, 21 Jun 2020 20:44:33: 21000000 INFO @ Sun, 21 Jun 2020 20:44:39: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 20:44:39: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 20:44:39: #1 total tags in treatment: 21857733 INFO @ Sun, 21 Jun 2020 20:44:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:44:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:44:39: #1 tags after filtering in treatment: 21857635 INFO @ Sun, 21 Jun 2020 20:44:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:44:39: #1 finished! INFO @ Sun, 21 Jun 2020 20:44:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:44:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:44:41: #2 number of paired peaks: 74 WARNING @ Sun, 21 Jun 2020 20:44:41: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:44:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4158197/SRX4158197.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。