Job ID = 6456884 SRX = SRX4047182 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:15:24 prefetch.2.10.7: 1) Downloading 'SRR7126163'... 2020-06-21T11:15:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:25:11 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:25:11 prefetch.2.10.7: 1) 'SRR7126163' was downloaded successfully 2020-06-21T11:25:11 prefetch.2.10.7: 'SRR7126163' has 0 unresolved dependencies Read 83805861 spots for SRR7126163/SRR7126163.sra Written 83805861 spots for SRR7126163/SRR7126163.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:58 83805861 reads; of these: 83805861 (100.00%) were unpaired; of these: 34571234 (41.25%) aligned 0 times 40828299 (48.72%) aligned exactly 1 time 8406328 (10.03%) aligned >1 times 58.75% overall alignment rate Time searching: 00:13:59 Overall time: 00:13:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 13400962 / 49234627 = 0.2722 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:54:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:54:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:54:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:54:39: 1000000 INFO @ Sun, 21 Jun 2020 20:54:45: 2000000 INFO @ Sun, 21 Jun 2020 20:54:50: 3000000 INFO @ Sun, 21 Jun 2020 20:54:55: 4000000 INFO @ Sun, 21 Jun 2020 20:55:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:55:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:55:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:55:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:55:06: 6000000 INFO @ Sun, 21 Jun 2020 20:55:10: 1000000 INFO @ Sun, 21 Jun 2020 20:55:12: 7000000 INFO @ Sun, 21 Jun 2020 20:55:16: 2000000 INFO @ Sun, 21 Jun 2020 20:55:18: 8000000 INFO @ Sun, 21 Jun 2020 20:55:22: 3000000 INFO @ Sun, 21 Jun 2020 20:55:24: 9000000 INFO @ Sun, 21 Jun 2020 20:55:28: 4000000 INFO @ Sun, 21 Jun 2020 20:55:29: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:55:33: 5000000 INFO @ Sun, 21 Jun 2020 20:55:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:55:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:55:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:55:35: 11000000 INFO @ Sun, 21 Jun 2020 20:55:40: 6000000 INFO @ Sun, 21 Jun 2020 20:55:40: 1000000 INFO @ Sun, 21 Jun 2020 20:55:42: 12000000 INFO @ Sun, 21 Jun 2020 20:55:46: 2000000 INFO @ Sun, 21 Jun 2020 20:55:46: 7000000 INFO @ Sun, 21 Jun 2020 20:55:48: 13000000 INFO @ Sun, 21 Jun 2020 20:55:52: 3000000 INFO @ Sun, 21 Jun 2020 20:55:53: 8000000 INFO @ Sun, 21 Jun 2020 20:55:54: 14000000 INFO @ Sun, 21 Jun 2020 20:55:58: 4000000 INFO @ Sun, 21 Jun 2020 20:55:59: 9000000 INFO @ Sun, 21 Jun 2020 20:56:00: 15000000 INFO @ Sun, 21 Jun 2020 20:56:03: 5000000 INFO @ Sun, 21 Jun 2020 20:56:05: 10000000 INFO @ Sun, 21 Jun 2020 20:56:06: 16000000 INFO @ Sun, 21 Jun 2020 20:56:09: 6000000 INFO @ Sun, 21 Jun 2020 20:56:11: 11000000 INFO @ Sun, 21 Jun 2020 20:56:12: 17000000 INFO @ Sun, 21 Jun 2020 20:56:15: 7000000 INFO @ Sun, 21 Jun 2020 20:56:17: 12000000 INFO @ Sun, 21 Jun 2020 20:56:18: 18000000 INFO @ Sun, 21 Jun 2020 20:56:21: 8000000 INFO @ Sun, 21 Jun 2020 20:56:23: 13000000 INFO @ Sun, 21 Jun 2020 20:56:24: 19000000 INFO @ Sun, 21 Jun 2020 20:56:27: 9000000 INFO @ Sun, 21 Jun 2020 20:56:29: 14000000 INFO @ Sun, 21 Jun 2020 20:56:30: 20000000 INFO @ Sun, 21 Jun 2020 20:56:33: 10000000 INFO @ Sun, 21 Jun 2020 20:56:35: 15000000 INFO @ Sun, 21 Jun 2020 20:56:36: 21000000 INFO @ Sun, 21 Jun 2020 20:56:39: 11000000 INFO @ Sun, 21 Jun 2020 20:56:41: 16000000 INFO @ Sun, 21 Jun 2020 20:56:42: 22000000 INFO @ Sun, 21 Jun 2020 20:56:45: 12000000 INFO @ Sun, 21 Jun 2020 20:56:47: 17000000 INFO @ Sun, 21 Jun 2020 20:56:48: 23000000 INFO @ Sun, 21 Jun 2020 20:56:51: 13000000 INFO @ Sun, 21 Jun 2020 20:56:53: 18000000 INFO @ Sun, 21 Jun 2020 20:56:54: 24000000 INFO @ Sun, 21 Jun 2020 20:56:57: 14000000 INFO @ Sun, 21 Jun 2020 20:56:59: 19000000 INFO @ Sun, 21 Jun 2020 20:57:00: 25000000 INFO @ Sun, 21 Jun 2020 20:57:03: 15000000 INFO @ Sun, 21 Jun 2020 20:57:05: 20000000 INFO @ Sun, 21 Jun 2020 20:57:06: 26000000 INFO @ Sun, 21 Jun 2020 20:57:08: 16000000 INFO @ Sun, 21 Jun 2020 20:57:11: 21000000 INFO @ Sun, 21 Jun 2020 20:57:12: 27000000 INFO @ Sun, 21 Jun 2020 20:57:14: 17000000 INFO @ Sun, 21 Jun 2020 20:57:17: 22000000 INFO @ Sun, 21 Jun 2020 20:57:18: 28000000 INFO @ Sun, 21 Jun 2020 20:57:20: 18000000 INFO @ Sun, 21 Jun 2020 20:57:23: 23000000 INFO @ Sun, 21 Jun 2020 20:57:24: 29000000 INFO @ Sun, 21 Jun 2020 20:57:26: 19000000 INFO @ Sun, 21 Jun 2020 20:57:29: 24000000 INFO @ Sun, 21 Jun 2020 20:57:30: 30000000 INFO @ Sun, 21 Jun 2020 20:57:32: 20000000 INFO @ Sun, 21 Jun 2020 20:57:35: 25000000 INFO @ Sun, 21 Jun 2020 20:57:36: 31000000 INFO @ Sun, 21 Jun 2020 20:57:38: 21000000 INFO @ Sun, 21 Jun 2020 20:57:41: 26000000 INFO @ Sun, 21 Jun 2020 20:57:42: 32000000 INFO @ Sun, 21 Jun 2020 20:57:44: 22000000 INFO @ Sun, 21 Jun 2020 20:57:47: 27000000 INFO @ Sun, 21 Jun 2020 20:57:48: 33000000 INFO @ Sun, 21 Jun 2020 20:57:50: 23000000 INFO @ Sun, 21 Jun 2020 20:57:52: 28000000 INFO @ Sun, 21 Jun 2020 20:57:54: 34000000 INFO @ Sun, 21 Jun 2020 20:57:56: 24000000 INFO @ Sun, 21 Jun 2020 20:57:58: 29000000 INFO @ Sun, 21 Jun 2020 20:58:00: 35000000 INFO @ Sun, 21 Jun 2020 20:58:01: 25000000 INFO @ Sun, 21 Jun 2020 20:58:05: 30000000 INFO @ Sun, 21 Jun 2020 20:58:05: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:58:05: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:58:05: #1 total tags in treatment: 35833665 INFO @ Sun, 21 Jun 2020 20:58:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:58:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:58:06: #1 tags after filtering in treatment: 35833661 INFO @ Sun, 21 Jun 2020 20:58:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:58:06: #1 finished! INFO @ Sun, 21 Jun 2020 20:58:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:58:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:58:07: 26000000 INFO @ Sun, 21 Jun 2020 20:58:08: #2 number of paired peaks: 71 WARNING @ Sun, 21 Jun 2020 20:58:08: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:58:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:58:11: 31000000 INFO @ Sun, 21 Jun 2020 20:58:13: 27000000 INFO @ Sun, 21 Jun 2020 20:58:17: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:58:19: 28000000 INFO @ Sun, 21 Jun 2020 20:58:23: 33000000 INFO @ Sun, 21 Jun 2020 20:58:25: 29000000 INFO @ Sun, 21 Jun 2020 20:58:29: 34000000 INFO @ Sun, 21 Jun 2020 20:58:32: 30000000 INFO @ Sun, 21 Jun 2020 20:58:35: 35000000 INFO @ Sun, 21 Jun 2020 20:58:38: 31000000 INFO @ Sun, 21 Jun 2020 20:58:41: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:58:41: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:58:41: #1 total tags in treatment: 35833665 INFO @ Sun, 21 Jun 2020 20:58:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:58:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:58:42: #1 tags after filtering in treatment: 35833661 INFO @ Sun, 21 Jun 2020 20:58:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:58:42: #1 finished! INFO @ Sun, 21 Jun 2020 20:58:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:58:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:58:44: 32000000 INFO @ Sun, 21 Jun 2020 20:58:44: #2 number of paired peaks: 71 WARNING @ Sun, 21 Jun 2020 20:58:44: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:58:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:58:49: 33000000 INFO @ Sun, 21 Jun 2020 20:58:55: 34000000 INFO @ Sun, 21 Jun 2020 20:59:01: 35000000 INFO @ Sun, 21 Jun 2020 20:59:06: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:59:06: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:59:06: #1 total tags in treatment: 35833665 INFO @ Sun, 21 Jun 2020 20:59:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:59:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:59:06: #1 tags after filtering in treatment: 35833661 INFO @ Sun, 21 Jun 2020 20:59:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:59:06: #1 finished! INFO @ Sun, 21 Jun 2020 20:59:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:59:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:59:09: #2 number of paired peaks: 71 WARNING @ Sun, 21 Jun 2020 20:59:09: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:59:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX4047182/SRX4047182.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。