Job ID = 6456873 SRX = SRX403441 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:27:27 prefetch.2.10.7: 1) Downloading 'SRR1066656'... 2020-06-21T11:27:27 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:31:36 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:31:36 prefetch.2.10.7: 1) 'SRR1066656' was downloaded successfully Read 33788440 spots for SRR1066656/SRR1066656.sra Written 33788440 spots for SRR1066656/SRR1066656.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:13 33788440 reads; of these: 33788440 (100.00%) were unpaired; of these: 1457698 (4.31%) aligned 0 times 24286079 (71.88%) aligned exactly 1 time 8044663 (23.81%) aligned >1 times 95.69% overall alignment rate Time searching: 00:07:13 Overall time: 00:07:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5636210 / 32330742 = 0.1743 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:46:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:46:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:46:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:46:40: 1000000 INFO @ Sun, 21 Jun 2020 20:46:45: 2000000 INFO @ Sun, 21 Jun 2020 20:46:50: 3000000 INFO @ Sun, 21 Jun 2020 20:46:55: 4000000 INFO @ Sun, 21 Jun 2020 20:47:00: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:47:05: 6000000 INFO @ Sun, 21 Jun 2020 20:47:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:47:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:47:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:47:10: 1000000 INFO @ Sun, 21 Jun 2020 20:47:10: 7000000 INFO @ Sun, 21 Jun 2020 20:47:14: 2000000 INFO @ Sun, 21 Jun 2020 20:47:15: 8000000 INFO @ Sun, 21 Jun 2020 20:47:18: 3000000 INFO @ Sun, 21 Jun 2020 20:47:20: 9000000 INFO @ Sun, 21 Jun 2020 20:47:23: 4000000 INFO @ Sun, 21 Jun 2020 20:47:25: 10000000 INFO @ Sun, 21 Jun 2020 20:47:27: 5000000 INFO @ Sun, 21 Jun 2020 20:47:30: 11000000 INFO @ Sun, 21 Jun 2020 20:47:32: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:47:35: 12000000 INFO @ Sun, 21 Jun 2020 20:47:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:47:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:47:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:47:36: 7000000 INFO @ Sun, 21 Jun 2020 20:47:40: 13000000 INFO @ Sun, 21 Jun 2020 20:47:40: 1000000 INFO @ Sun, 21 Jun 2020 20:47:40: 8000000 INFO @ Sun, 21 Jun 2020 20:47:44: 2000000 INFO @ Sun, 21 Jun 2020 20:47:44: 14000000 INFO @ Sun, 21 Jun 2020 20:47:45: 9000000 INFO @ Sun, 21 Jun 2020 20:47:48: 3000000 INFO @ Sun, 21 Jun 2020 20:47:49: 10000000 INFO @ Sun, 21 Jun 2020 20:47:49: 15000000 INFO @ Sun, 21 Jun 2020 20:47:53: 4000000 INFO @ Sun, 21 Jun 2020 20:47:53: 11000000 INFO @ Sun, 21 Jun 2020 20:47:54: 16000000 INFO @ Sun, 21 Jun 2020 20:47:57: 5000000 INFO @ Sun, 21 Jun 2020 20:47:58: 12000000 INFO @ Sun, 21 Jun 2020 20:47:59: 17000000 INFO @ Sun, 21 Jun 2020 20:48:01: 6000000 INFO @ Sun, 21 Jun 2020 20:48:02: 13000000 INFO @ Sun, 21 Jun 2020 20:48:04: 18000000 INFO @ Sun, 21 Jun 2020 20:48:06: 7000000 INFO @ Sun, 21 Jun 2020 20:48:06: 14000000 INFO @ Sun, 21 Jun 2020 20:48:09: 19000000 INFO @ Sun, 21 Jun 2020 20:48:10: 8000000 INFO @ Sun, 21 Jun 2020 20:48:11: 15000000 INFO @ Sun, 21 Jun 2020 20:48:14: 20000000 INFO @ Sun, 21 Jun 2020 20:48:14: 9000000 INFO @ Sun, 21 Jun 2020 20:48:15: 16000000 INFO @ Sun, 21 Jun 2020 20:48:18: 21000000 INFO @ Sun, 21 Jun 2020 20:48:19: 10000000 INFO @ Sun, 21 Jun 2020 20:48:19: 17000000 INFO @ Sun, 21 Jun 2020 20:48:23: 22000000 INFO @ Sun, 21 Jun 2020 20:48:23: 11000000 INFO @ Sun, 21 Jun 2020 20:48:24: 18000000 INFO @ Sun, 21 Jun 2020 20:48:28: 12000000 INFO @ Sun, 21 Jun 2020 20:48:28: 19000000 INFO @ Sun, 21 Jun 2020 20:48:29: 23000000 INFO @ Sun, 21 Jun 2020 20:48:32: 13000000 INFO @ Sun, 21 Jun 2020 20:48:32: 20000000 INFO @ Sun, 21 Jun 2020 20:48:33: 24000000 INFO @ Sun, 21 Jun 2020 20:48:36: 14000000 INFO @ Sun, 21 Jun 2020 20:48:37: 21000000 INFO @ Sun, 21 Jun 2020 20:48:38: 25000000 INFO @ Sun, 21 Jun 2020 20:48:41: 15000000 INFO @ Sun, 21 Jun 2020 20:48:41: 22000000 INFO @ Sun, 21 Jun 2020 20:48:43: 26000000 INFO @ Sun, 21 Jun 2020 20:48:45: 16000000 INFO @ Sun, 21 Jun 2020 20:48:46: 23000000 INFO @ Sun, 21 Jun 2020 20:48:47: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 20:48:47: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 20:48:47: #1 total tags in treatment: 26694532 INFO @ Sun, 21 Jun 2020 20:48:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:48:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:48:48: #1 tags after filtering in treatment: 26694527 INFO @ Sun, 21 Jun 2020 20:48:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:48:48: #1 finished! INFO @ Sun, 21 Jun 2020 20:48:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:48:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:48:49: 17000000 INFO @ Sun, 21 Jun 2020 20:48:49: #2 number of paired peaks: 72 WARNING @ Sun, 21 Jun 2020 20:48:49: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:48:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:48:50: 24000000 INFO @ Sun, 21 Jun 2020 20:48:54: 18000000 INFO @ Sun, 21 Jun 2020 20:48:54: 25000000 INFO @ Sun, 21 Jun 2020 20:48:58: 19000000 INFO @ Sun, 21 Jun 2020 20:48:59: 26000000 INFO @ Sun, 21 Jun 2020 20:49:02: 20000000 INFO @ Sun, 21 Jun 2020 20:49:02: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 20:49:02: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 20:49:02: #1 total tags in treatment: 26694532 INFO @ Sun, 21 Jun 2020 20:49:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:49:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:49:03: #1 tags after filtering in treatment: 26694527 INFO @ Sun, 21 Jun 2020 20:49:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:49:03: #1 finished! INFO @ Sun, 21 Jun 2020 20:49:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:49:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:49:05: #2 number of paired peaks: 72 WARNING @ Sun, 21 Jun 2020 20:49:05: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:49:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:49:06: 21000000 INFO @ Sun, 21 Jun 2020 20:49:11: 22000000 INFO @ Sun, 21 Jun 2020 20:49:15: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:49:20: 24000000 INFO @ Sun, 21 Jun 2020 20:49:24: 25000000 INFO @ Sun, 21 Jun 2020 20:49:29: 26000000 INFO @ Sun, 21 Jun 2020 20:49:32: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 20:49:32: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 20:49:32: #1 total tags in treatment: 26694532 INFO @ Sun, 21 Jun 2020 20:49:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:49:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:49:32: #1 tags after filtering in treatment: 26694527 INFO @ Sun, 21 Jun 2020 20:49:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:49:32: #1 finished! INFO @ Sun, 21 Jun 2020 20:49:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:49:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:49:34: #2 number of paired peaks: 72 WARNING @ Sun, 21 Jun 2020 20:49:34: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:49:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX403441/SRX403441.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。