Job ID = 6456855 SRX = SRX3989969 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:02:46 prefetch.2.10.7: 1) Downloading 'SRR7059010'... 2020-06-21T11:02:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:06:09 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:06:09 prefetch.2.10.7: 1) 'SRR7059010' was downloaded successfully 2020-06-21T11:06:09 prefetch.2.10.7: 'SRR7059010' has 0 unresolved dependencies Read 19902490 spots for SRR7059010/SRR7059010.sra Written 19902490 spots for SRR7059010/SRR7059010.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:28 19902490 reads; of these: 19902490 (100.00%) were unpaired; of these: 1340821 (6.74%) aligned 0 times 14822629 (74.48%) aligned exactly 1 time 3739040 (18.79%) aligned >1 times 93.26% overall alignment rate Time searching: 00:04:28 Overall time: 00:04:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3908388 / 18561669 = 0.2106 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:16:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:16:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:16:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:16:32: 1000000 INFO @ Sun, 21 Jun 2020 20:16:38: 2000000 INFO @ Sun, 21 Jun 2020 20:16:43: 3000000 INFO @ Sun, 21 Jun 2020 20:16:49: 4000000 INFO @ Sun, 21 Jun 2020 20:16:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:16:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:16:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:16:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:17:00: 6000000 INFO @ Sun, 21 Jun 2020 20:17:03: 1000000 INFO @ Sun, 21 Jun 2020 20:17:06: 7000000 INFO @ Sun, 21 Jun 2020 20:17:09: 2000000 INFO @ Sun, 21 Jun 2020 20:17:13: 8000000 INFO @ Sun, 21 Jun 2020 20:17:15: 3000000 INFO @ Sun, 21 Jun 2020 20:17:19: 9000000 INFO @ Sun, 21 Jun 2020 20:17:21: 4000000 INFO @ Sun, 21 Jun 2020 20:17:25: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:17:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:17:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:17:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:17:27: 5000000 INFO @ Sun, 21 Jun 2020 20:17:31: 11000000 INFO @ Sun, 21 Jun 2020 20:17:34: 6000000 INFO @ Sun, 21 Jun 2020 20:17:34: 1000000 INFO @ Sun, 21 Jun 2020 20:17:38: 12000000 INFO @ Sun, 21 Jun 2020 20:17:41: 7000000 INFO @ Sun, 21 Jun 2020 20:17:42: 2000000 INFO @ Sun, 21 Jun 2020 20:17:45: 13000000 INFO @ Sun, 21 Jun 2020 20:17:47: 8000000 INFO @ Sun, 21 Jun 2020 20:17:49: 3000000 INFO @ Sun, 21 Jun 2020 20:17:52: 14000000 INFO @ Sun, 21 Jun 2020 20:17:54: 9000000 INFO @ Sun, 21 Jun 2020 20:17:56: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 20:17:56: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 20:17:56: #1 total tags in treatment: 14653281 INFO @ Sun, 21 Jun 2020 20:17:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:17:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:17:57: 4000000 INFO @ Sun, 21 Jun 2020 20:17:57: #1 tags after filtering in treatment: 14653277 INFO @ Sun, 21 Jun 2020 20:17:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:17:57: #1 finished! INFO @ Sun, 21 Jun 2020 20:17:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:17:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:17:58: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 20:17:58: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:17:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:18:01: 10000000 INFO @ Sun, 21 Jun 2020 20:18:04: 5000000 INFO @ Sun, 21 Jun 2020 20:18:07: 11000000 INFO @ Sun, 21 Jun 2020 20:18:11: 6000000 INFO @ Sun, 21 Jun 2020 20:18:14: 12000000 INFO @ Sun, 21 Jun 2020 20:18:19: 7000000 INFO @ Sun, 21 Jun 2020 20:18:21: 13000000 INFO @ Sun, 21 Jun 2020 20:18:26: 8000000 INFO @ Sun, 21 Jun 2020 20:18:28: 14000000 INFO @ Sun, 21 Jun 2020 20:18:32: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 20:18:32: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 20:18:32: #1 total tags in treatment: 14653281 INFO @ Sun, 21 Jun 2020 20:18:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:18:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:18:33: #1 tags after filtering in treatment: 14653277 INFO @ Sun, 21 Jun 2020 20:18:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:18:33: #1 finished! INFO @ Sun, 21 Jun 2020 20:18:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:18:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:18:33: 9000000 INFO @ Sun, 21 Jun 2020 20:18:34: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 20:18:34: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:18:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:18:40: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:18:47: 11000000 INFO @ Sun, 21 Jun 2020 20:18:55: 12000000 INFO @ Sun, 21 Jun 2020 20:19:02: 13000000 INFO @ Sun, 21 Jun 2020 20:19:08: 14000000 INFO @ Sun, 21 Jun 2020 20:19:13: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 20:19:13: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 20:19:13: #1 total tags in treatment: 14653281 INFO @ Sun, 21 Jun 2020 20:19:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:19:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:19:14: #1 tags after filtering in treatment: 14653277 INFO @ Sun, 21 Jun 2020 20:19:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:19:14: #1 finished! INFO @ Sun, 21 Jun 2020 20:19:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:19:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:19:14: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 20:19:14: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:19:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3989969/SRX3989969.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。