Job ID = 6456854 SRX = SRX3981736 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:06:09 prefetch.2.10.7: 1) Downloading 'SRR7050620'... 2020-06-21T11:06:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:07:27 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:07:28 prefetch.2.10.7: 'SRR7050620' is valid 2020-06-21T11:07:28 prefetch.2.10.7: 1) 'SRR7050620' was downloaded successfully 2020-06-21T11:07:28 prefetch.2.10.7: 'SRR7050620' has 0 unresolved dependencies Read 14380553 spots for SRR7050620/SRR7050620.sra Written 14380553 spots for SRR7050620/SRR7050620.sra 2020-06-21T11:08:27 prefetch.2.10.7: 1) Downloading 'SRR7050621'... 2020-06-21T11:08:27 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:10:16 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:10:16 prefetch.2.10.7: 'SRR7050621' is valid 2020-06-21T11:10:16 prefetch.2.10.7: 1) 'SRR7050621' was downloaded successfully 2020-06-21T11:10:16 prefetch.2.10.7: 'SRR7050621' has 0 unresolved dependencies Read 14570629 spots for SRR7050621/SRR7050621.sra Written 14570629 spots for SRR7050621/SRR7050621.sra 2020-06-21T11:11:16 prefetch.2.10.7: 1) Downloading 'SRR7050622'... 2020-06-21T11:11:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:13:35 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:13:36 prefetch.2.10.7: 'SRR7050622' is valid 2020-06-21T11:13:36 prefetch.2.10.7: 1) 'SRR7050622' was downloaded successfully 2020-06-21T11:13:36 prefetch.2.10.7: 'SRR7050622' has 0 unresolved dependencies Read 14614020 spots for SRR7050622/SRR7050622.sra Written 14614020 spots for SRR7050622/SRR7050622.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:57 43565202 reads; of these: 43565202 (100.00%) were unpaired; of these: 1200282 (2.76%) aligned 0 times 33831889 (77.66%) aligned exactly 1 time 8533031 (19.59%) aligned >1 times 97.24% overall alignment rate Time searching: 00:11:57 Overall time: 00:11:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 15130413 / 42364920 = 0.3571 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:35:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:35:36: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:35:36: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:35:42: 1000000 INFO @ Sun, 21 Jun 2020 20:35:47: 2000000 INFO @ Sun, 21 Jun 2020 20:35:52: 3000000 INFO @ Sun, 21 Jun 2020 20:35:57: 4000000 INFO @ Sun, 21 Jun 2020 20:36:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:36:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:36:07: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:36:07: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:36:08: 6000000 INFO @ Sun, 21 Jun 2020 20:36:12: 1000000 INFO @ Sun, 21 Jun 2020 20:36:13: 7000000 INFO @ Sun, 21 Jun 2020 20:36:18: 2000000 INFO @ Sun, 21 Jun 2020 20:36:19: 8000000 INFO @ Sun, 21 Jun 2020 20:36:24: 3000000 INFO @ Sun, 21 Jun 2020 20:36:24: 9000000 INFO @ Sun, 21 Jun 2020 20:36:30: 10000000 INFO @ Sun, 21 Jun 2020 20:36:30: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:36:35: 11000000 INFO @ Sun, 21 Jun 2020 20:36:36: 5000000 INFO @ Sun, 21 Jun 2020 20:36:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:36:36: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:36:36: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:36:41: 12000000 INFO @ Sun, 21 Jun 2020 20:36:41: 6000000 INFO @ Sun, 21 Jun 2020 20:36:42: 1000000 INFO @ Sun, 21 Jun 2020 20:36:46: 13000000 INFO @ Sun, 21 Jun 2020 20:36:47: 7000000 INFO @ Sun, 21 Jun 2020 20:36:48: 2000000 INFO @ Sun, 21 Jun 2020 20:36:52: 14000000 INFO @ Sun, 21 Jun 2020 20:36:53: 8000000 INFO @ Sun, 21 Jun 2020 20:36:54: 3000000 INFO @ Sun, 21 Jun 2020 20:36:57: 15000000 INFO @ Sun, 21 Jun 2020 20:36:59: 9000000 INFO @ Sun, 21 Jun 2020 20:37:00: 4000000 INFO @ Sun, 21 Jun 2020 20:37:03: 16000000 INFO @ Sun, 21 Jun 2020 20:37:05: 10000000 INFO @ Sun, 21 Jun 2020 20:37:05: 5000000 INFO @ Sun, 21 Jun 2020 20:37:08: 17000000 INFO @ Sun, 21 Jun 2020 20:37:10: 11000000 INFO @ Sun, 21 Jun 2020 20:37:11: 6000000 INFO @ Sun, 21 Jun 2020 20:37:14: 18000000 INFO @ Sun, 21 Jun 2020 20:37:16: 12000000 INFO @ Sun, 21 Jun 2020 20:37:17: 7000000 INFO @ Sun, 21 Jun 2020 20:37:20: 19000000 INFO @ Sun, 21 Jun 2020 20:37:22: 13000000 INFO @ Sun, 21 Jun 2020 20:37:23: 8000000 INFO @ Sun, 21 Jun 2020 20:37:25: 20000000 INFO @ Sun, 21 Jun 2020 20:37:28: 14000000 INFO @ Sun, 21 Jun 2020 20:37:28: 9000000 INFO @ Sun, 21 Jun 2020 20:37:31: 21000000 INFO @ Sun, 21 Jun 2020 20:37:34: 10000000 INFO @ Sun, 21 Jun 2020 20:37:34: 15000000 INFO @ Sun, 21 Jun 2020 20:37:37: 22000000 INFO @ Sun, 21 Jun 2020 20:37:40: 11000000 INFO @ Sun, 21 Jun 2020 20:37:40: 16000000 INFO @ Sun, 21 Jun 2020 20:37:43: 23000000 INFO @ Sun, 21 Jun 2020 20:37:46: 12000000 INFO @ Sun, 21 Jun 2020 20:37:46: 17000000 INFO @ Sun, 21 Jun 2020 20:37:48: 24000000 INFO @ Sun, 21 Jun 2020 20:37:51: 13000000 INFO @ Sun, 21 Jun 2020 20:37:52: 18000000 INFO @ Sun, 21 Jun 2020 20:37:54: 25000000 INFO @ Sun, 21 Jun 2020 20:37:56: 14000000 INFO @ Sun, 21 Jun 2020 20:37:57: 19000000 INFO @ Sun, 21 Jun 2020 20:37:59: 26000000 INFO @ Sun, 21 Jun 2020 20:38:02: 15000000 INFO @ Sun, 21 Jun 2020 20:38:02: 20000000 INFO @ Sun, 21 Jun 2020 20:38:05: 27000000 INFO @ Sun, 21 Jun 2020 20:38:06: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:38:06: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:38:06: #1 total tags in treatment: 27234507 INFO @ Sun, 21 Jun 2020 20:38:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:38:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:38:07: #1 tags after filtering in treatment: 27234440 INFO @ Sun, 21 Jun 2020 20:38:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:38:07: #1 finished! INFO @ Sun, 21 Jun 2020 20:38:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:38:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:38:08: 16000000 INFO @ Sun, 21 Jun 2020 20:38:08: 21000000 INFO @ Sun, 21 Jun 2020 20:38:09: #2 number of paired peaks: 3648 INFO @ Sun, 21 Jun 2020 20:38:09: start model_add_line... INFO @ Sun, 21 Jun 2020 20:38:10: start X-correlation... INFO @ Sun, 21 Jun 2020 20:38:10: end of X-cor INFO @ Sun, 21 Jun 2020 20:38:10: #2 finished! INFO @ Sun, 21 Jun 2020 20:38:10: #2 predicted fragment length is 216 bps INFO @ Sun, 21 Jun 2020 20:38:10: #2 alternative fragment length(s) may be 4,216 bps INFO @ Sun, 21 Jun 2020 20:38:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.05_model.r INFO @ Sun, 21 Jun 2020 20:38:10: #3 Call peaks... INFO @ Sun, 21 Jun 2020 20:38:10: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 20:38:13: 17000000 INFO @ Sun, 21 Jun 2020 20:38:13: 22000000 INFO @ Sun, 21 Jun 2020 20:38:18: 18000000 INFO @ Sun, 21 Jun 2020 20:38:19: 23000000 INFO @ Sun, 21 Jun 2020 20:38:24: 19000000 INFO @ Sun, 21 Jun 2020 20:38:25: 24000000 INFO @ Sun, 21 Jun 2020 20:38:29: 20000000 INFO @ Sun, 21 Jun 2020 20:38:30: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:38:35: 21000000 INFO @ Sun, 21 Jun 2020 20:38:36: 26000000 INFO @ Sun, 21 Jun 2020 20:38:40: 22000000 INFO @ Sun, 21 Jun 2020 20:38:41: 27000000 INFO @ Sun, 21 Jun 2020 20:38:43: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:38:43: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:38:43: #1 total tags in treatment: 27234507 INFO @ Sun, 21 Jun 2020 20:38:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:38:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:38:44: #1 tags after filtering in treatment: 27234440 INFO @ Sun, 21 Jun 2020 20:38:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:38:44: #1 finished! INFO @ Sun, 21 Jun 2020 20:38:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:38:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:38:46: #2 number of paired peaks: 3648 INFO @ Sun, 21 Jun 2020 20:38:46: start model_add_line... INFO @ Sun, 21 Jun 2020 20:38:46: 23000000 INFO @ Sun, 21 Jun 2020 20:38:46: start X-correlation... INFO @ Sun, 21 Jun 2020 20:38:46: end of X-cor INFO @ Sun, 21 Jun 2020 20:38:46: #2 finished! INFO @ Sun, 21 Jun 2020 20:38:46: #2 predicted fragment length is 216 bps INFO @ Sun, 21 Jun 2020 20:38:46: #2 alternative fragment length(s) may be 4,216 bps INFO @ Sun, 21 Jun 2020 20:38:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.10_model.r INFO @ Sun, 21 Jun 2020 20:38:46: #3 Call peaks... INFO @ Sun, 21 Jun 2020 20:38:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 20:38:52: 24000000 INFO @ Sun, 21 Jun 2020 20:38:57: 25000000 INFO @ Sun, 21 Jun 2020 20:39:02: 26000000 INFO @ Sun, 21 Jun 2020 20:39:08: 27000000 INFO @ Sun, 21 Jun 2020 20:39:09: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:39:09: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:39:09: #1 total tags in treatment: 27234507 INFO @ Sun, 21 Jun 2020 20:39:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:39:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:39:10: #1 tags after filtering in treatment: 27234440 INFO @ Sun, 21 Jun 2020 20:39:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:39:10: #1 finished! INFO @ Sun, 21 Jun 2020 20:39:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:39:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:39:12: #2 number of paired peaks: 3648 INFO @ Sun, 21 Jun 2020 20:39:12: start model_add_line... INFO @ Sun, 21 Jun 2020 20:39:13: start X-correlation... INFO @ Sun, 21 Jun 2020 20:39:13: end of X-cor INFO @ Sun, 21 Jun 2020 20:39:13: #2 finished! INFO @ Sun, 21 Jun 2020 20:39:13: #2 predicted fragment length is 216 bps INFO @ Sun, 21 Jun 2020 20:39:13: #2 alternative fragment length(s) may be 4,216 bps INFO @ Sun, 21 Jun 2020 20:39:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.20_model.r INFO @ Sun, 21 Jun 2020 20:39:13: #3 Call peaks... INFO @ Sun, 21 Jun 2020 20:39:13: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:39:29: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 20:39:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.05_peaks.xls INFO @ Sun, 21 Jun 2020 20:39:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.05_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 20:40:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.05_summits.bed INFO @ Sun, 21 Jun 2020 20:40:00: Done! pass1 - making usageList (254 chroms): 2 millis pass2 - checking and writing primary data (9570 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:40:11: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 20:40:37: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 20:40:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.10_peaks.xls INFO @ Sun, 21 Jun 2020 20:40:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.10_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 20:40:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.10_summits.bed INFO @ Sun, 21 Jun 2020 20:40:41: Done! pass1 - making usageList (202 chroms): 2 millis pass2 - checking and writing primary data (7300 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:41:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.20_peaks.xls INFO @ Sun, 21 Jun 2020 20:41:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.20_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 20:41:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3981736/SRX3981736.20_summits.bed INFO @ Sun, 21 Jun 2020 20:41:07: Done! pass1 - making usageList (165 chroms): 2 millis pass2 - checking and writing primary data (4723 records, 4 fields): 12 millis CompletedMACS2peakCalling