Job ID = 6529674 SRX = SRX3981639 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:54 44128995 reads; of these: 44128995 (100.00%) were unpaired; of these: 1788108 (4.05%) aligned 0 times 36307317 (82.28%) aligned exactly 1 time 6033570 (13.67%) aligned >1 times 95.95% overall alignment rate Time searching: 00:09:54 Overall time: 00:09:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11092559 / 42340887 = 0.2620 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 02:47:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 02:47:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 02:47:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 02:47:20: 1000000 INFO @ Tue, 30 Jun 2020 02:47:26: 2000000 INFO @ Tue, 30 Jun 2020 02:47:32: 3000000 INFO @ Tue, 30 Jun 2020 02:47:37: 4000000 INFO @ Tue, 30 Jun 2020 02:47:43: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 02:47:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 02:47:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 02:47:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 02:47:49: 6000000 INFO @ Tue, 30 Jun 2020 02:47:51: 1000000 INFO @ Tue, 30 Jun 2020 02:47:55: 7000000 INFO @ Tue, 30 Jun 2020 02:47:58: 2000000 INFO @ Tue, 30 Jun 2020 02:48:02: 8000000 INFO @ Tue, 30 Jun 2020 02:48:04: 3000000 INFO @ Tue, 30 Jun 2020 02:48:08: 9000000 INFO @ Tue, 30 Jun 2020 02:48:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 02:48:14: 10000000 INFO @ Tue, 30 Jun 2020 02:48:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 02:48:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 02:48:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 02:48:17: 5000000 INFO @ Tue, 30 Jun 2020 02:48:21: 11000000 INFO @ Tue, 30 Jun 2020 02:48:21: 1000000 INFO @ Tue, 30 Jun 2020 02:48:23: 6000000 INFO @ Tue, 30 Jun 2020 02:48:27: 12000000 INFO @ Tue, 30 Jun 2020 02:48:28: 2000000 INFO @ Tue, 30 Jun 2020 02:48:30: 7000000 INFO @ Tue, 30 Jun 2020 02:48:33: 13000000 INFO @ Tue, 30 Jun 2020 02:48:34: 3000000 INFO @ Tue, 30 Jun 2020 02:48:36: 8000000 INFO @ Tue, 30 Jun 2020 02:48:40: 14000000 INFO @ Tue, 30 Jun 2020 02:48:41: 4000000 INFO @ Tue, 30 Jun 2020 02:48:42: 9000000 INFO @ Tue, 30 Jun 2020 02:48:46: 15000000 INFO @ Tue, 30 Jun 2020 02:48:47: 5000000 INFO @ Tue, 30 Jun 2020 02:48:49: 10000000 INFO @ Tue, 30 Jun 2020 02:48:52: 16000000 INFO @ Tue, 30 Jun 2020 02:48:54: 6000000 INFO @ Tue, 30 Jun 2020 02:48:55: 11000000 INFO @ Tue, 30 Jun 2020 02:48:59: 17000000 INFO @ Tue, 30 Jun 2020 02:49:00: 7000000 INFO @ Tue, 30 Jun 2020 02:49:02: 12000000 INFO @ Tue, 30 Jun 2020 02:49:05: 18000000 INFO @ Tue, 30 Jun 2020 02:49:06: 8000000 INFO @ Tue, 30 Jun 2020 02:49:08: 13000000 INFO @ Tue, 30 Jun 2020 02:49:12: 19000000 INFO @ Tue, 30 Jun 2020 02:49:13: 9000000 INFO @ Tue, 30 Jun 2020 02:49:14: 14000000 INFO @ Tue, 30 Jun 2020 02:49:18: 20000000 INFO @ Tue, 30 Jun 2020 02:49:19: 10000000 INFO @ Tue, 30 Jun 2020 02:49:21: 15000000 INFO @ Tue, 30 Jun 2020 02:49:24: 21000000 INFO @ Tue, 30 Jun 2020 02:49:25: 11000000 INFO @ Tue, 30 Jun 2020 02:49:27: 16000000 INFO @ Tue, 30 Jun 2020 02:49:30: 22000000 INFO @ Tue, 30 Jun 2020 02:49:32: 12000000 INFO @ Tue, 30 Jun 2020 02:49:33: 17000000 INFO @ Tue, 30 Jun 2020 02:49:37: 23000000 INFO @ Tue, 30 Jun 2020 02:49:38: 13000000 INFO @ Tue, 30 Jun 2020 02:49:39: 18000000 INFO @ Tue, 30 Jun 2020 02:49:43: 24000000 INFO @ Tue, 30 Jun 2020 02:49:44: 14000000 INFO @ Tue, 30 Jun 2020 02:49:46: 19000000 INFO @ Tue, 30 Jun 2020 02:49:49: 25000000 INFO @ Tue, 30 Jun 2020 02:49:50: 15000000 INFO @ Tue, 30 Jun 2020 02:49:52: 20000000 INFO @ Tue, 30 Jun 2020 02:49:56: 26000000 INFO @ Tue, 30 Jun 2020 02:49:57: 16000000 INFO @ Tue, 30 Jun 2020 02:49:58: 21000000 INFO @ Tue, 30 Jun 2020 02:50:02: 27000000 INFO @ Tue, 30 Jun 2020 02:50:03: 17000000 INFO @ Tue, 30 Jun 2020 02:50:05: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 02:50:09: 28000000 INFO @ Tue, 30 Jun 2020 02:50:09: 18000000 INFO @ Tue, 30 Jun 2020 02:50:11: 23000000 INFO @ Tue, 30 Jun 2020 02:50:15: 29000000 INFO @ Tue, 30 Jun 2020 02:50:16: 19000000 INFO @ Tue, 30 Jun 2020 02:50:18: 24000000 INFO @ Tue, 30 Jun 2020 02:50:22: 30000000 INFO @ Tue, 30 Jun 2020 02:50:22: 20000000 INFO @ Tue, 30 Jun 2020 02:50:24: 25000000 INFO @ Tue, 30 Jun 2020 02:50:28: 31000000 INFO @ Tue, 30 Jun 2020 02:50:29: 21000000 INFO @ Tue, 30 Jun 2020 02:50:30: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 02:50:30: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 02:50:30: #1 total tags in treatment: 31248328 INFO @ Tue, 30 Jun 2020 02:50:30: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 02:50:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 02:50:30: 26000000 INFO @ Tue, 30 Jun 2020 02:50:31: #1 tags after filtering in treatment: 31248271 INFO @ Tue, 30 Jun 2020 02:50:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 02:50:31: #1 finished! INFO @ Tue, 30 Jun 2020 02:50:31: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 02:50:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 02:50:33: #2 number of paired peaks: 2076 INFO @ Tue, 30 Jun 2020 02:50:33: start model_add_line... INFO @ Tue, 30 Jun 2020 02:50:33: start X-correlation... INFO @ Tue, 30 Jun 2020 02:50:33: end of X-cor INFO @ Tue, 30 Jun 2020 02:50:33: #2 finished! INFO @ Tue, 30 Jun 2020 02:50:33: #2 predicted fragment length is 108 bps INFO @ Tue, 30 Jun 2020 02:50:33: #2 alternative fragment length(s) may be 2,108,130 bps INFO @ Tue, 30 Jun 2020 02:50:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.05_model.r INFO @ Tue, 30 Jun 2020 02:50:33: #3 Call peaks... INFO @ Tue, 30 Jun 2020 02:50:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 02:50:35: 22000000 INFO @ Tue, 30 Jun 2020 02:50:37: 27000000 INFO @ Tue, 30 Jun 2020 02:50:41: 23000000 INFO @ Tue, 30 Jun 2020 02:50:44: 28000000 INFO @ Tue, 30 Jun 2020 02:50:48: 24000000 INFO @ Tue, 30 Jun 2020 02:50:50: 29000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 02:50:54: 25000000 INFO @ Tue, 30 Jun 2020 02:50:56: 30000000 INFO @ Tue, 30 Jun 2020 02:51:01: 26000000 INFO @ Tue, 30 Jun 2020 02:51:03: 31000000 INFO @ Tue, 30 Jun 2020 02:51:05: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 02:51:05: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 02:51:05: #1 total tags in treatment: 31248328 INFO @ Tue, 30 Jun 2020 02:51:05: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 02:51:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 02:51:05: #1 tags after filtering in treatment: 31248271 INFO @ Tue, 30 Jun 2020 02:51:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 02:51:05: #1 finished! INFO @ Tue, 30 Jun 2020 02:51:05: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 02:51:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 02:51:07: 27000000 INFO @ Tue, 30 Jun 2020 02:51:07: #2 number of paired peaks: 2076 INFO @ Tue, 30 Jun 2020 02:51:07: start model_add_line... INFO @ Tue, 30 Jun 2020 02:51:08: start X-correlation... INFO @ Tue, 30 Jun 2020 02:51:08: end of X-cor INFO @ Tue, 30 Jun 2020 02:51:08: #2 finished! INFO @ Tue, 30 Jun 2020 02:51:08: #2 predicted fragment length is 108 bps INFO @ Tue, 30 Jun 2020 02:51:08: #2 alternative fragment length(s) may be 2,108,130 bps INFO @ Tue, 30 Jun 2020 02:51:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.10_model.r INFO @ Tue, 30 Jun 2020 02:51:08: #3 Call peaks... INFO @ Tue, 30 Jun 2020 02:51:08: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 02:51:13: 28000000 INFO @ Tue, 30 Jun 2020 02:51:19: 29000000 INFO @ Tue, 30 Jun 2020 02:51:24: 30000000 INFO @ Tue, 30 Jun 2020 02:51:30: 31000000 INFO @ Tue, 30 Jun 2020 02:51:32: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 02:51:32: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 02:51:32: #1 total tags in treatment: 31248328 INFO @ Tue, 30 Jun 2020 02:51:32: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 02:51:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 02:51:33: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 02:51:33: #1 tags after filtering in treatment: 31248271 INFO @ Tue, 30 Jun 2020 02:51:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 02:51:33: #1 finished! INFO @ Tue, 30 Jun 2020 02:51:33: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 02:51:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 02:51:35: #2 number of paired peaks: 2076 INFO @ Tue, 30 Jun 2020 02:51:35: start model_add_line... INFO @ Tue, 30 Jun 2020 02:51:35: start X-correlation... INFO @ Tue, 30 Jun 2020 02:51:35: end of X-cor INFO @ Tue, 30 Jun 2020 02:51:35: #2 finished! INFO @ Tue, 30 Jun 2020 02:51:35: #2 predicted fragment length is 108 bps INFO @ Tue, 30 Jun 2020 02:51:35: #2 alternative fragment length(s) may be 2,108,130 bps INFO @ Tue, 30 Jun 2020 02:51:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.20_model.r INFO @ Tue, 30 Jun 2020 02:51:35: #3 Call peaks... INFO @ Tue, 30 Jun 2020 02:51:35: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 02:52:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.05_peaks.xls INFO @ Tue, 30 Jun 2020 02:52:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.05_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 02:52:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.05_summits.bed INFO @ Tue, 30 Jun 2020 02:52:00: Done! pass1 - making usageList (193 chroms): 2 millis pass2 - checking and writing primary data (10877 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 02:52:06: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 02:52:33: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.10_peaks.xls INFO @ Tue, 30 Jun 2020 02:52:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.10_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 02:52:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.10_summits.bed INFO @ Tue, 30 Jun 2020 02:52:33: Done! pass1 - making usageList (135 chroms): 2 millis pass2 - checking and writing primary data (4737 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 02:52:34: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 02:53:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.20_peaks.xls INFO @ Tue, 30 Jun 2020 02:53:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.20_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 02:53:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX3981639/SRX3981639.20_summits.bed INFO @ Tue, 30 Jun 2020 02:53:00: Done! pass1 - making usageList (76 chroms): 1 millis pass2 - checking and writing primary data (1211 records, 4 fields): 5 millis CompletedMACS2peakCalling