Job ID = 6456707 SRX = SRX3981615 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:57:46 prefetch.2.10.7: 1) Downloading 'SRR7050229'... 2020-06-21T10:57:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:59:42 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:59:43 prefetch.2.10.7: 'SRR7050229' is valid 2020-06-21T10:59:43 prefetch.2.10.7: 1) 'SRR7050229' was downloaded successfully 2020-06-21T10:59:43 prefetch.2.10.7: 'SRR7050229' has 0 unresolved dependencies Read 15538549 spots for SRR7050229/SRR7050229.sra Written 15538549 spots for SRR7050229/SRR7050229.sra 2020-06-21T11:00:44 prefetch.2.10.7: 1) Downloading 'SRR7050230'... 2020-06-21T11:00:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:02:21 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:02:22 prefetch.2.10.7: 'SRR7050230' is valid 2020-06-21T11:02:22 prefetch.2.10.7: 1) 'SRR7050230' was downloaded successfully 2020-06-21T11:02:22 prefetch.2.10.7: 'SRR7050230' has 0 unresolved dependencies Read 15132011 spots for SRR7050230/SRR7050230.sra Written 15132011 spots for SRR7050230/SRR7050230.sra 2020-06-21T11:03:20 prefetch.2.10.7: 1) Downloading 'SRR7050231'... 2020-06-21T11:03:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:04:59 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:04:59 prefetch.2.10.7: 'SRR7050231' is valid 2020-06-21T11:04:59 prefetch.2.10.7: 1) 'SRR7050231' was downloaded successfully 2020-06-21T11:04:59 prefetch.2.10.7: 'SRR7050231' has 0 unresolved dependencies Read 15517808 spots for SRR7050231/SRR7050231.sra Written 15517808 spots for SRR7050231/SRR7050231.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:06 46188368 reads; of these: 46188368 (100.00%) were unpaired; of these: 1307695 (2.83%) aligned 0 times 34517607 (74.73%) aligned exactly 1 time 10363066 (22.44%) aligned >1 times 97.17% overall alignment rate Time searching: 00:12:06 Overall time: 00:12:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 6442239 / 44880673 = 0.1435 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:29:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:29:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:29:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:29:08: 1000000 INFO @ Sun, 21 Jun 2020 20:29:14: 2000000 INFO @ Sun, 21 Jun 2020 20:29:20: 3000000 INFO @ Sun, 21 Jun 2020 20:29:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:29:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:29:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:29:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:29:33: 5000000 INFO @ Sun, 21 Jun 2020 20:29:39: 1000000 INFO @ Sun, 21 Jun 2020 20:29:40: 6000000 INFO @ Sun, 21 Jun 2020 20:29:47: 2000000 INFO @ Sun, 21 Jun 2020 20:29:47: 7000000 INFO @ Sun, 21 Jun 2020 20:29:55: 3000000 INFO @ Sun, 21 Jun 2020 20:29:55: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:30:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:30:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:30:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:30:02: 9000000 INFO @ Sun, 21 Jun 2020 20:30:02: 4000000 INFO @ Sun, 21 Jun 2020 20:30:09: 1000000 INFO @ Sun, 21 Jun 2020 20:30:10: 10000000 INFO @ Sun, 21 Jun 2020 20:30:10: 5000000 INFO @ Sun, 21 Jun 2020 20:30:16: 2000000 INFO @ Sun, 21 Jun 2020 20:30:17: 11000000 INFO @ Sun, 21 Jun 2020 20:30:18: 6000000 INFO @ Sun, 21 Jun 2020 20:30:24: 3000000 INFO @ Sun, 21 Jun 2020 20:30:24: 12000000 INFO @ Sun, 21 Jun 2020 20:30:25: 7000000 INFO @ Sun, 21 Jun 2020 20:30:31: 4000000 INFO @ Sun, 21 Jun 2020 20:30:32: 13000000 INFO @ Sun, 21 Jun 2020 20:30:33: 8000000 INFO @ Sun, 21 Jun 2020 20:30:38: 5000000 INFO @ Sun, 21 Jun 2020 20:30:39: 14000000 INFO @ Sun, 21 Jun 2020 20:30:41: 9000000 INFO @ Sun, 21 Jun 2020 20:30:46: 6000000 INFO @ Sun, 21 Jun 2020 20:30:47: 15000000 INFO @ Sun, 21 Jun 2020 20:30:48: 10000000 INFO @ Sun, 21 Jun 2020 20:30:53: 7000000 INFO @ Sun, 21 Jun 2020 20:30:54: 16000000 INFO @ Sun, 21 Jun 2020 20:30:55: 11000000 INFO @ Sun, 21 Jun 2020 20:31:01: 8000000 INFO @ Sun, 21 Jun 2020 20:31:01: 17000000 INFO @ Sun, 21 Jun 2020 20:31:03: 12000000 INFO @ Sun, 21 Jun 2020 20:31:08: 9000000 INFO @ Sun, 21 Jun 2020 20:31:09: 18000000 INFO @ Sun, 21 Jun 2020 20:31:10: 13000000 INFO @ Sun, 21 Jun 2020 20:31:15: 10000000 INFO @ Sun, 21 Jun 2020 20:31:16: 19000000 INFO @ Sun, 21 Jun 2020 20:31:18: 14000000 INFO @ Sun, 21 Jun 2020 20:31:22: 11000000 INFO @ Sun, 21 Jun 2020 20:31:23: 20000000 INFO @ Sun, 21 Jun 2020 20:31:25: 15000000 INFO @ Sun, 21 Jun 2020 20:31:30: 12000000 INFO @ Sun, 21 Jun 2020 20:31:31: 21000000 INFO @ Sun, 21 Jun 2020 20:31:33: 16000000 INFO @ Sun, 21 Jun 2020 20:31:37: 13000000 INFO @ Sun, 21 Jun 2020 20:31:38: 22000000 INFO @ Sun, 21 Jun 2020 20:31:40: 17000000 INFO @ Sun, 21 Jun 2020 20:31:44: 14000000 INFO @ Sun, 21 Jun 2020 20:31:45: 23000000 INFO @ Sun, 21 Jun 2020 20:31:48: 18000000 INFO @ Sun, 21 Jun 2020 20:31:51: 15000000 INFO @ Sun, 21 Jun 2020 20:31:52: 24000000 INFO @ Sun, 21 Jun 2020 20:31:56: 19000000 INFO @ Sun, 21 Jun 2020 20:31:58: 16000000 INFO @ Sun, 21 Jun 2020 20:32:00: 25000000 INFO @ Sun, 21 Jun 2020 20:32:03: 20000000 INFO @ Sun, 21 Jun 2020 20:32:05: 17000000 INFO @ Sun, 21 Jun 2020 20:32:07: 26000000 INFO @ Sun, 21 Jun 2020 20:32:11: 21000000 INFO @ Sun, 21 Jun 2020 20:32:13: 18000000 INFO @ Sun, 21 Jun 2020 20:32:14: 27000000 INFO @ Sun, 21 Jun 2020 20:32:18: 22000000 INFO @ Sun, 21 Jun 2020 20:32:20: 19000000 INFO @ Sun, 21 Jun 2020 20:32:21: 28000000 INFO @ Sun, 21 Jun 2020 20:32:26: 23000000 INFO @ Sun, 21 Jun 2020 20:32:27: 20000000 INFO @ Sun, 21 Jun 2020 20:32:28: 29000000 INFO @ Sun, 21 Jun 2020 20:32:34: 24000000 INFO @ Sun, 21 Jun 2020 20:32:34: 21000000 INFO @ Sun, 21 Jun 2020 20:32:36: 30000000 INFO @ Sun, 21 Jun 2020 20:32:41: 22000000 INFO @ Sun, 21 Jun 2020 20:32:42: 25000000 INFO @ Sun, 21 Jun 2020 20:32:43: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:32:49: 23000000 INFO @ Sun, 21 Jun 2020 20:32:49: 26000000 INFO @ Sun, 21 Jun 2020 20:32:50: 32000000 INFO @ Sun, 21 Jun 2020 20:32:56: 24000000 INFO @ Sun, 21 Jun 2020 20:32:57: 27000000 INFO @ Sun, 21 Jun 2020 20:32:58: 33000000 INFO @ Sun, 21 Jun 2020 20:33:03: 25000000 INFO @ Sun, 21 Jun 2020 20:33:05: 34000000 INFO @ Sun, 21 Jun 2020 20:33:05: 28000000 INFO @ Sun, 21 Jun 2020 20:33:10: 26000000 INFO @ Sun, 21 Jun 2020 20:33:12: 35000000 INFO @ Sun, 21 Jun 2020 20:33:14: 29000000 INFO @ Sun, 21 Jun 2020 20:33:18: 27000000 INFO @ Sun, 21 Jun 2020 20:33:20: 36000000 INFO @ Sun, 21 Jun 2020 20:33:22: 30000000 INFO @ Sun, 21 Jun 2020 20:33:25: 28000000 INFO @ Sun, 21 Jun 2020 20:33:27: 37000000 INFO @ Sun, 21 Jun 2020 20:33:31: 31000000 INFO @ Sun, 21 Jun 2020 20:33:33: 29000000 INFO @ Sun, 21 Jun 2020 20:33:35: 38000000 INFO @ Sun, 21 Jun 2020 20:33:38: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:33:38: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:33:38: #1 total tags in treatment: 38438434 INFO @ Sun, 21 Jun 2020 20:33:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:33:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:33:39: 32000000 INFO @ Sun, 21 Jun 2020 20:33:39: #1 tags after filtering in treatment: 38438385 INFO @ Sun, 21 Jun 2020 20:33:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:33:39: #1 finished! INFO @ Sun, 21 Jun 2020 20:33:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:33:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:33:40: 30000000 INFO @ Sun, 21 Jun 2020 20:33:42: #2 number of paired peaks: 77 WARNING @ Sun, 21 Jun 2020 20:33:42: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:33:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:33:47: 31000000 INFO @ Sun, 21 Jun 2020 20:33:49: 33000000 INFO @ Sun, 21 Jun 2020 20:33:54: 32000000 INFO @ Sun, 21 Jun 2020 20:33:58: 34000000 INFO @ Sun, 21 Jun 2020 20:34:02: 33000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:34:06: 35000000 INFO @ Sun, 21 Jun 2020 20:34:10: 34000000 INFO @ Sun, 21 Jun 2020 20:34:15: 36000000 INFO @ Sun, 21 Jun 2020 20:34:17: 35000000 INFO @ Sun, 21 Jun 2020 20:34:24: 37000000 INFO @ Sun, 21 Jun 2020 20:34:25: 36000000 INFO @ Sun, 21 Jun 2020 20:34:32: 37000000 INFO @ Sun, 21 Jun 2020 20:34:33: 38000000 INFO @ Sun, 21 Jun 2020 20:34:37: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:34:37: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:34:37: #1 total tags in treatment: 38438434 INFO @ Sun, 21 Jun 2020 20:34:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:34:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:34:38: #1 tags after filtering in treatment: 38438385 INFO @ Sun, 21 Jun 2020 20:34:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:34:38: #1 finished! INFO @ Sun, 21 Jun 2020 20:34:38: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:34:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:34:40: 38000000 INFO @ Sun, 21 Jun 2020 20:34:40: #2 number of paired peaks: 77 WARNING @ Sun, 21 Jun 2020 20:34:40: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:34:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:34:43: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:34:43: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:34:43: #1 total tags in treatment: 38438434 INFO @ Sun, 21 Jun 2020 20:34:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:34:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:34:44: #1 tags after filtering in treatment: 38438385 INFO @ Sun, 21 Jun 2020 20:34:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:34:44: #1 finished! INFO @ Sun, 21 Jun 2020 20:34:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:34:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:34:46: #2 number of paired peaks: 77 WARNING @ Sun, 21 Jun 2020 20:34:46: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:34:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3981615/SRX3981615.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling