Job ID = 6456607 SRX = SRX370362 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:13:24 prefetch.2.10.7: 1) Downloading 'SRR1020541'... 2020-06-21T11:13:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:00:04 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:00:04 prefetch.2.10.7: 1) 'SRR1020541' was downloaded successfully Read 146552705 spots for SRR1020541/SRR1020541.sra Written 146552705 spots for SRR1020541/SRR1020541.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:40:54 146552705 reads; of these: 146552705 (100.00%) were unpaired; of these: 42050729 (28.69%) aligned 0 times 96914025 (66.13%) aligned exactly 1 time 7587951 (5.18%) aligned >1 times 71.31% overall alignment rate Time searching: 00:40:54 Overall time: 00:40:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 62243407 / 104501976 = 0.5956 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:25:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:25:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:25:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:25:23: 1000000 INFO @ Sun, 21 Jun 2020 22:25:31: 2000000 INFO @ Sun, 21 Jun 2020 22:25:39: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:25:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:25:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:25:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:25:47: 4000000 INFO @ Sun, 21 Jun 2020 22:25:53: 1000000 INFO @ Sun, 21 Jun 2020 22:25:55: 5000000 INFO @ Sun, 21 Jun 2020 22:26:01: 2000000 INFO @ Sun, 21 Jun 2020 22:26:03: 6000000 INFO @ Sun, 21 Jun 2020 22:26:09: 3000000 INFO @ Sun, 21 Jun 2020 22:26:11: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:26:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:26:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:26:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:26:17: 4000000 INFO @ Sun, 21 Jun 2020 22:26:19: 8000000 INFO @ Sun, 21 Jun 2020 22:26:24: 1000000 INFO @ Sun, 21 Jun 2020 22:26:25: 5000000 INFO @ Sun, 21 Jun 2020 22:26:27: 9000000 INFO @ Sun, 21 Jun 2020 22:26:32: 2000000 INFO @ Sun, 21 Jun 2020 22:26:33: 6000000 INFO @ Sun, 21 Jun 2020 22:26:36: 10000000 INFO @ Sun, 21 Jun 2020 22:26:40: 3000000 INFO @ Sun, 21 Jun 2020 22:26:41: 7000000 INFO @ Sun, 21 Jun 2020 22:26:44: 11000000 INFO @ Sun, 21 Jun 2020 22:26:49: 8000000 INFO @ Sun, 21 Jun 2020 22:26:49: 4000000 INFO @ Sun, 21 Jun 2020 22:26:52: 12000000 INFO @ Sun, 21 Jun 2020 22:26:57: 9000000 INFO @ Sun, 21 Jun 2020 22:26:57: 5000000 INFO @ Sun, 21 Jun 2020 22:27:00: 13000000 INFO @ Sun, 21 Jun 2020 22:27:05: 10000000 INFO @ Sun, 21 Jun 2020 22:27:05: 6000000 INFO @ Sun, 21 Jun 2020 22:27:08: 14000000 INFO @ Sun, 21 Jun 2020 22:27:13: 11000000 INFO @ Sun, 21 Jun 2020 22:27:13: 7000000 INFO @ Sun, 21 Jun 2020 22:27:16: 15000000 INFO @ Sun, 21 Jun 2020 22:27:21: 12000000 INFO @ Sun, 21 Jun 2020 22:27:21: 8000000 INFO @ Sun, 21 Jun 2020 22:27:24: 16000000 INFO @ Sun, 21 Jun 2020 22:27:29: 13000000 INFO @ Sun, 21 Jun 2020 22:27:30: 9000000 INFO @ Sun, 21 Jun 2020 22:27:32: 17000000 INFO @ Sun, 21 Jun 2020 22:27:37: 14000000 INFO @ Sun, 21 Jun 2020 22:27:38: 10000000 INFO @ Sun, 21 Jun 2020 22:27:40: 18000000 INFO @ Sun, 21 Jun 2020 22:27:45: 15000000 INFO @ Sun, 21 Jun 2020 22:27:46: 11000000 INFO @ Sun, 21 Jun 2020 22:27:49: 19000000 INFO @ Sun, 21 Jun 2020 22:27:53: 16000000 INFO @ Sun, 21 Jun 2020 22:27:54: 12000000 INFO @ Sun, 21 Jun 2020 22:27:57: 20000000 INFO @ Sun, 21 Jun 2020 22:28:01: 17000000 INFO @ Sun, 21 Jun 2020 22:28:02: 13000000 INFO @ Sun, 21 Jun 2020 22:28:05: 21000000 INFO @ Sun, 21 Jun 2020 22:28:09: 18000000 INFO @ Sun, 21 Jun 2020 22:28:11: 14000000 INFO @ Sun, 21 Jun 2020 22:28:13: 22000000 INFO @ Sun, 21 Jun 2020 22:28:17: 19000000 INFO @ Sun, 21 Jun 2020 22:28:19: 15000000 INFO @ Sun, 21 Jun 2020 22:28:22: 23000000 INFO @ Sun, 21 Jun 2020 22:28:26: 20000000 INFO @ Sun, 21 Jun 2020 22:28:27: 16000000 INFO @ Sun, 21 Jun 2020 22:28:30: 24000000 INFO @ Sun, 21 Jun 2020 22:28:34: 21000000 INFO @ Sun, 21 Jun 2020 22:28:35: 17000000 INFO @ Sun, 21 Jun 2020 22:28:38: 25000000 INFO @ Sun, 21 Jun 2020 22:28:42: 22000000 INFO @ Sun, 21 Jun 2020 22:28:43: 18000000 INFO @ Sun, 21 Jun 2020 22:28:46: 26000000 INFO @ Sun, 21 Jun 2020 22:28:50: 23000000 INFO @ Sun, 21 Jun 2020 22:28:52: 19000000 INFO @ Sun, 21 Jun 2020 22:28:54: 27000000 INFO @ Sun, 21 Jun 2020 22:28:58: 24000000 INFO @ Sun, 21 Jun 2020 22:29:00: 20000000 INFO @ Sun, 21 Jun 2020 22:29:02: 28000000 INFO @ Sun, 21 Jun 2020 22:29:06: 25000000 INFO @ Sun, 21 Jun 2020 22:29:08: 21000000 INFO @ Sun, 21 Jun 2020 22:29:10: 29000000 INFO @ Sun, 21 Jun 2020 22:29:14: 26000000 INFO @ Sun, 21 Jun 2020 22:29:17: 22000000 INFO @ Sun, 21 Jun 2020 22:29:19: 30000000 INFO @ Sun, 21 Jun 2020 22:29:22: 27000000 INFO @ Sun, 21 Jun 2020 22:29:25: 23000000 INFO @ Sun, 21 Jun 2020 22:29:27: 31000000 INFO @ Sun, 21 Jun 2020 22:29:31: 28000000 INFO @ Sun, 21 Jun 2020 22:29:33: 24000000 INFO @ Sun, 21 Jun 2020 22:29:34: 32000000 INFO @ Sun, 21 Jun 2020 22:29:39: 29000000 INFO @ Sun, 21 Jun 2020 22:29:41: 25000000 INFO @ Sun, 21 Jun 2020 22:29:42: 33000000 INFO @ Sun, 21 Jun 2020 22:29:47: 30000000 INFO @ Sun, 21 Jun 2020 22:29:49: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:29:50: 34000000 INFO @ Sun, 21 Jun 2020 22:29:55: 31000000 INFO @ Sun, 21 Jun 2020 22:29:57: 27000000 INFO @ Sun, 21 Jun 2020 22:29:59: 35000000 INFO @ Sun, 21 Jun 2020 22:30:02: 32000000 INFO @ Sun, 21 Jun 2020 22:30:05: 28000000 INFO @ Sun, 21 Jun 2020 22:30:07: 36000000 INFO @ Sun, 21 Jun 2020 22:30:10: 33000000 INFO @ Sun, 21 Jun 2020 22:30:13: 29000000 INFO @ Sun, 21 Jun 2020 22:30:15: 37000000 INFO @ Sun, 21 Jun 2020 22:30:18: 34000000 INFO @ Sun, 21 Jun 2020 22:30:21: 30000000 INFO @ Sun, 21 Jun 2020 22:30:22: 38000000 INFO @ Sun, 21 Jun 2020 22:30:26: 35000000 INFO @ Sun, 21 Jun 2020 22:30:29: 31000000 INFO @ Sun, 21 Jun 2020 22:30:30: 39000000 INFO @ Sun, 21 Jun 2020 22:30:34: 36000000 INFO @ Sun, 21 Jun 2020 22:30:37: 32000000 INFO @ Sun, 21 Jun 2020 22:30:38: 40000000 INFO @ Sun, 21 Jun 2020 22:30:42: 37000000 INFO @ Sun, 21 Jun 2020 22:30:44: 33000000 INFO @ Sun, 21 Jun 2020 22:30:46: 41000000 INFO @ Sun, 21 Jun 2020 22:30:49: 38000000 INFO @ Sun, 21 Jun 2020 22:30:52: 34000000 INFO @ Sun, 21 Jun 2020 22:30:53: 42000000 INFO @ Sun, 21 Jun 2020 22:30:56: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:30:56: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:30:56: #1 total tags in treatment: 42258569 INFO @ Sun, 21 Jun 2020 22:30:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:30:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:30:57: 39000000 INFO @ Sun, 21 Jun 2020 22:30:58: #1 tags after filtering in treatment: 42258430 INFO @ Sun, 21 Jun 2020 22:30:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:30:58: #1 finished! INFO @ Sun, 21 Jun 2020 22:30:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:30:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:31:00: 35000000 INFO @ Sun, 21 Jun 2020 22:31:00: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 22:31:00: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:31:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:31:05: 40000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:31:08: 36000000 INFO @ Sun, 21 Jun 2020 22:31:12: 41000000 INFO @ Sun, 21 Jun 2020 22:31:16: 37000000 INFO @ Sun, 21 Jun 2020 22:31:20: 42000000 INFO @ Sun, 21 Jun 2020 22:31:23: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:31:23: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:31:23: #1 total tags in treatment: 42258569 INFO @ Sun, 21 Jun 2020 22:31:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:31:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:31:23: 38000000 INFO @ Sun, 21 Jun 2020 22:31:24: #1 tags after filtering in treatment: 42258430 INFO @ Sun, 21 Jun 2020 22:31:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:31:24: #1 finished! INFO @ Sun, 21 Jun 2020 22:31:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:31:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:31:27: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 22:31:27: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:31:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:31:31: 39000000 INFO @ Sun, 21 Jun 2020 22:31:38: 40000000 INFO @ Sun, 21 Jun 2020 22:31:45: 41000000 INFO @ Sun, 21 Jun 2020 22:31:52: 42000000 INFO @ Sun, 21 Jun 2020 22:31:54: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:31:54: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:31:54: #1 total tags in treatment: 42258569 INFO @ Sun, 21 Jun 2020 22:31:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:31:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:31:56: #1 tags after filtering in treatment: 42258430 INFO @ Sun, 21 Jun 2020 22:31:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:31:56: #1 finished! INFO @ Sun, 21 Jun 2020 22:31:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:31:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:31:58: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 22:31:58: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:31:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370362/SRX370362.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling