Job ID = 6456606 SRX = SRX370361 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:00:01 prefetch.2.10.7: 1) Downloading 'SRR1020540'... 2020-06-21T11:00:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:33:04 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:33:04 prefetch.2.10.7: 1) 'SRR1020540' was downloaded successfully Read 105881026 spots for SRR1020540/SRR1020540.sra Written 105881026 spots for SRR1020540/SRR1020540.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:52 105881026 reads; of these: 105881026 (100.00%) were unpaired; of these: 15642084 (14.77%) aligned 0 times 82662040 (78.07%) aligned exactly 1 time 7576902 (7.16%) aligned >1 times 85.23% overall alignment rate Time searching: 00:32:52 Overall time: 00:32:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 55322369 / 90238942 = 0.6131 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:39:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:39:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:39:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:39:17: 1000000 INFO @ Sun, 21 Jun 2020 21:39:23: 2000000 INFO @ Sun, 21 Jun 2020 21:39:29: 3000000 INFO @ Sun, 21 Jun 2020 21:39:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:39:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:39:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:39:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:39:42: 5000000 INFO @ Sun, 21 Jun 2020 21:39:49: 6000000 INFO @ Sun, 21 Jun 2020 21:39:49: 1000000 INFO @ Sun, 21 Jun 2020 21:39:56: 7000000 INFO @ Sun, 21 Jun 2020 21:39:57: 2000000 INFO @ Sun, 21 Jun 2020 21:40:04: 8000000 INFO @ Sun, 21 Jun 2020 21:40:05: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:40:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:40:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:40:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:40:11: 9000000 INFO @ Sun, 21 Jun 2020 21:40:13: 4000000 INFO @ Sun, 21 Jun 2020 21:40:18: 1000000 INFO @ Sun, 21 Jun 2020 21:40:19: 10000000 INFO @ Sun, 21 Jun 2020 21:40:21: 5000000 INFO @ Sun, 21 Jun 2020 21:40:25: 2000000 INFO @ Sun, 21 Jun 2020 21:40:26: 11000000 INFO @ Sun, 21 Jun 2020 21:40:29: 6000000 INFO @ Sun, 21 Jun 2020 21:40:33: 3000000 INFO @ Sun, 21 Jun 2020 21:40:34: 12000000 INFO @ Sun, 21 Jun 2020 21:40:36: 7000000 INFO @ Sun, 21 Jun 2020 21:40:40: 4000000 INFO @ Sun, 21 Jun 2020 21:40:41: 13000000 INFO @ Sun, 21 Jun 2020 21:40:44: 8000000 INFO @ Sun, 21 Jun 2020 21:40:47: 5000000 INFO @ Sun, 21 Jun 2020 21:40:49: 14000000 INFO @ Sun, 21 Jun 2020 21:40:52: 9000000 INFO @ Sun, 21 Jun 2020 21:40:55: 6000000 INFO @ Sun, 21 Jun 2020 21:40:56: 15000000 INFO @ Sun, 21 Jun 2020 21:41:00: 10000000 INFO @ Sun, 21 Jun 2020 21:41:02: 7000000 INFO @ Sun, 21 Jun 2020 21:41:03: 16000000 INFO @ Sun, 21 Jun 2020 21:41:08: 11000000 INFO @ Sun, 21 Jun 2020 21:41:09: 8000000 INFO @ Sun, 21 Jun 2020 21:41:11: 17000000 INFO @ Sun, 21 Jun 2020 21:41:16: 12000000 INFO @ Sun, 21 Jun 2020 21:41:17: 9000000 INFO @ Sun, 21 Jun 2020 21:41:19: 18000000 INFO @ Sun, 21 Jun 2020 21:41:24: 13000000 INFO @ Sun, 21 Jun 2020 21:41:24: 10000000 INFO @ Sun, 21 Jun 2020 21:41:26: 19000000 INFO @ Sun, 21 Jun 2020 21:41:32: 11000000 INFO @ Sun, 21 Jun 2020 21:41:33: 14000000 INFO @ Sun, 21 Jun 2020 21:41:34: 20000000 INFO @ Sun, 21 Jun 2020 21:41:39: 12000000 INFO @ Sun, 21 Jun 2020 21:41:41: 21000000 INFO @ Sun, 21 Jun 2020 21:41:41: 15000000 INFO @ Sun, 21 Jun 2020 21:41:47: 13000000 INFO @ Sun, 21 Jun 2020 21:41:49: 22000000 INFO @ Sun, 21 Jun 2020 21:41:50: 16000000 INFO @ Sun, 21 Jun 2020 21:41:54: 14000000 INFO @ Sun, 21 Jun 2020 21:41:56: 23000000 INFO @ Sun, 21 Jun 2020 21:41:59: 17000000 INFO @ Sun, 21 Jun 2020 21:42:02: 15000000 INFO @ Sun, 21 Jun 2020 21:42:04: 24000000 INFO @ Sun, 21 Jun 2020 21:42:07: 18000000 INFO @ Sun, 21 Jun 2020 21:42:09: 16000000 INFO @ Sun, 21 Jun 2020 21:42:11: 25000000 INFO @ Sun, 21 Jun 2020 21:42:16: 19000000 INFO @ Sun, 21 Jun 2020 21:42:17: 17000000 INFO @ Sun, 21 Jun 2020 21:42:19: 26000000 INFO @ Sun, 21 Jun 2020 21:42:24: 20000000 INFO @ Sun, 21 Jun 2020 21:42:25: 18000000 INFO @ Sun, 21 Jun 2020 21:42:26: 27000000 INFO @ Sun, 21 Jun 2020 21:42:32: 19000000 INFO @ Sun, 21 Jun 2020 21:42:32: 21000000 INFO @ Sun, 21 Jun 2020 21:42:34: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:42:39: 20000000 INFO @ Sun, 21 Jun 2020 21:42:40: 22000000 INFO @ Sun, 21 Jun 2020 21:42:41: 29000000 INFO @ Sun, 21 Jun 2020 21:42:47: 21000000 INFO @ Sun, 21 Jun 2020 21:42:49: 23000000 INFO @ Sun, 21 Jun 2020 21:42:49: 30000000 INFO @ Sun, 21 Jun 2020 21:42:54: 22000000 INFO @ Sun, 21 Jun 2020 21:42:56: 31000000 INFO @ Sun, 21 Jun 2020 21:42:57: 24000000 INFO @ Sun, 21 Jun 2020 21:43:02: 23000000 INFO @ Sun, 21 Jun 2020 21:43:04: 32000000 INFO @ Sun, 21 Jun 2020 21:43:05: 25000000 INFO @ Sun, 21 Jun 2020 21:43:09: 24000000 INFO @ Sun, 21 Jun 2020 21:43:11: 33000000 INFO @ Sun, 21 Jun 2020 21:43:13: 26000000 INFO @ Sun, 21 Jun 2020 21:43:17: 25000000 INFO @ Sun, 21 Jun 2020 21:43:19: 34000000 INFO @ Sun, 21 Jun 2020 21:43:22: 27000000 INFO @ Sun, 21 Jun 2020 21:43:24: 26000000 INFO @ Sun, 21 Jun 2020 21:43:26: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 21:43:26: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 21:43:26: #1 total tags in treatment: 34916573 INFO @ Sun, 21 Jun 2020 21:43:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:43:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:43:27: #1 tags after filtering in treatment: 34916407 INFO @ Sun, 21 Jun 2020 21:43:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:43:27: #1 finished! INFO @ Sun, 21 Jun 2020 21:43:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:43:27: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 21:43:29: #2 number of paired peaks: 96 WARNING @ Sun, 21 Jun 2020 21:43:29: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:43:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:43:30: 28000000 INFO @ Sun, 21 Jun 2020 21:43:32: 27000000 INFO @ Sun, 21 Jun 2020 21:43:38: 29000000 INFO @ Sun, 21 Jun 2020 21:43:39: 28000000 INFO @ Sun, 21 Jun 2020 21:43:46: 29000000 INFO @ Sun, 21 Jun 2020 21:43:46: 30000000 INFO @ Sun, 21 Jun 2020 21:43:54: 30000000 INFO @ Sun, 21 Jun 2020 21:43:55: 31000000 INFO @ Sun, 21 Jun 2020 21:44:01: 31000000 INFO @ Sun, 21 Jun 2020 21:44:03: 32000000 INFO @ Sun, 21 Jun 2020 21:44:08: 32000000 INFO @ Sun, 21 Jun 2020 21:44:11: 33000000 INFO @ Sun, 21 Jun 2020 21:44:15: 33000000 INFO @ Sun, 21 Jun 2020 21:44:19: 34000000 INFO @ Sun, 21 Jun 2020 21:44:23: 34000000 INFO @ Sun, 21 Jun 2020 21:44:27: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 21:44:27: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 21:44:27: #1 total tags in treatment: 34916573 INFO @ Sun, 21 Jun 2020 21:44:27: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:44:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:44:27: #1 tags after filtering in treatment: 34916407 INFO @ Sun, 21 Jun 2020 21:44:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:44:27: #1 finished! INFO @ Sun, 21 Jun 2020 21:44:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:44:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:44:30: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 21:44:30: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 21:44:30: #1 total tags in treatment: 34916573 INFO @ Sun, 21 Jun 2020 21:44:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:44:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:44:30: #2 number of paired peaks: 96 WARNING @ Sun, 21 Jun 2020 21:44:30: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:44:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:44:30: #1 tags after filtering in treatment: 34916407 INFO @ Sun, 21 Jun 2020 21:44:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:44:30: #1 finished! INFO @ Sun, 21 Jun 2020 21:44:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:44:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:44:32: #2 number of paired peaks: 96 WARNING @ Sun, 21 Jun 2020 21:44:32: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:44:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370361/SRX370361.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling