Job ID = 6456605 SRX = SRX370360 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:01:31 prefetch.2.10.7: 1) Downloading 'SRR1020539'... 2020-06-21T11:01:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:39:15 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:39:15 prefetch.2.10.7: 1) 'SRR1020539' was downloaded successfully Read 142082745 spots for SRR1020539/SRR1020539.sra Written 142082745 spots for SRR1020539/SRR1020539.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:40:11 142082745 reads; of these: 142082745 (100.00%) were unpaired; of these: 37438713 (26.35%) aligned 0 times 96813897 (68.14%) aligned exactly 1 time 7830135 (5.51%) aligned >1 times 73.65% overall alignment rate Time searching: 00:40:11 Overall time: 00:40:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 63198739 / 104644032 = 0.6039 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:57:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:57:47: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:57:47: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:57:55: 1000000 INFO @ Sun, 21 Jun 2020 21:58:03: 2000000 INFO @ Sun, 21 Jun 2020 21:58:10: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:58:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:58:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:58:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:58:19: 4000000 INFO @ Sun, 21 Jun 2020 21:58:25: 1000000 INFO @ Sun, 21 Jun 2020 21:58:28: 5000000 INFO @ Sun, 21 Jun 2020 21:58:33: 2000000 INFO @ Sun, 21 Jun 2020 21:58:37: 6000000 INFO @ Sun, 21 Jun 2020 21:58:42: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:58:45: 7000000 INFO @ Sun, 21 Jun 2020 21:58:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:58:47: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:58:47: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:58:50: 4000000 INFO @ Sun, 21 Jun 2020 21:58:55: 8000000 INFO @ Sun, 21 Jun 2020 21:58:55: 1000000 INFO @ Sun, 21 Jun 2020 21:58:59: 5000000 INFO @ Sun, 21 Jun 2020 21:59:04: 9000000 INFO @ Sun, 21 Jun 2020 21:59:04: 2000000 INFO @ Sun, 21 Jun 2020 21:59:08: 6000000 INFO @ Sun, 21 Jun 2020 21:59:13: 3000000 INFO @ Sun, 21 Jun 2020 21:59:13: 10000000 INFO @ Sun, 21 Jun 2020 21:59:16: 7000000 INFO @ Sun, 21 Jun 2020 21:59:22: 4000000 INFO @ Sun, 21 Jun 2020 21:59:22: 11000000 INFO @ Sun, 21 Jun 2020 21:59:25: 8000000 INFO @ Sun, 21 Jun 2020 21:59:30: 5000000 INFO @ Sun, 21 Jun 2020 21:59:31: 12000000 INFO @ Sun, 21 Jun 2020 21:59:34: 9000000 INFO @ Sun, 21 Jun 2020 21:59:39: 6000000 INFO @ Sun, 21 Jun 2020 21:59:40: 13000000 INFO @ Sun, 21 Jun 2020 21:59:43: 10000000 INFO @ Sun, 21 Jun 2020 21:59:48: 7000000 INFO @ Sun, 21 Jun 2020 21:59:49: 14000000 INFO @ Sun, 21 Jun 2020 21:59:52: 11000000 INFO @ Sun, 21 Jun 2020 21:59:57: 8000000 INFO @ Sun, 21 Jun 2020 21:59:58: 15000000 INFO @ Sun, 21 Jun 2020 22:00:00: 12000000 INFO @ Sun, 21 Jun 2020 22:00:05: 9000000 INFO @ Sun, 21 Jun 2020 22:00:07: 16000000 INFO @ Sun, 21 Jun 2020 22:00:09: 13000000 INFO @ Sun, 21 Jun 2020 22:00:14: 10000000 INFO @ Sun, 21 Jun 2020 22:00:16: 17000000 INFO @ Sun, 21 Jun 2020 22:00:18: 14000000 INFO @ Sun, 21 Jun 2020 22:00:23: 11000000 INFO @ Sun, 21 Jun 2020 22:00:25: 18000000 INFO @ Sun, 21 Jun 2020 22:00:27: 15000000 INFO @ Sun, 21 Jun 2020 22:00:32: 12000000 INFO @ Sun, 21 Jun 2020 22:00:34: 19000000 INFO @ Sun, 21 Jun 2020 22:00:36: 16000000 INFO @ Sun, 21 Jun 2020 22:00:41: 13000000 INFO @ Sun, 21 Jun 2020 22:00:43: 20000000 INFO @ Sun, 21 Jun 2020 22:00:45: 17000000 INFO @ Sun, 21 Jun 2020 22:00:50: 14000000 INFO @ Sun, 21 Jun 2020 22:00:52: 21000000 INFO @ Sun, 21 Jun 2020 22:00:54: 18000000 INFO @ Sun, 21 Jun 2020 22:00:59: 15000000 INFO @ Sun, 21 Jun 2020 22:01:01: 22000000 INFO @ Sun, 21 Jun 2020 22:01:03: 19000000 INFO @ Sun, 21 Jun 2020 22:01:08: 16000000 INFO @ Sun, 21 Jun 2020 22:01:11: 23000000 INFO @ Sun, 21 Jun 2020 22:01:12: 20000000 INFO @ Sun, 21 Jun 2020 22:01:17: 17000000 INFO @ Sun, 21 Jun 2020 22:01:20: 24000000 INFO @ Sun, 21 Jun 2020 22:01:20: 21000000 INFO @ Sun, 21 Jun 2020 22:01:25: 18000000 INFO @ Sun, 21 Jun 2020 22:01:29: 25000000 INFO @ Sun, 21 Jun 2020 22:01:29: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:01:34: 19000000 INFO @ Sun, 21 Jun 2020 22:01:38: 26000000 INFO @ Sun, 21 Jun 2020 22:01:38: 23000000 INFO @ Sun, 21 Jun 2020 22:01:43: 20000000 INFO @ Sun, 21 Jun 2020 22:01:47: 24000000 INFO @ Sun, 21 Jun 2020 22:01:47: 27000000 INFO @ Sun, 21 Jun 2020 22:01:52: 21000000 INFO @ Sun, 21 Jun 2020 22:01:55: 25000000 INFO @ Sun, 21 Jun 2020 22:01:56: 28000000 INFO @ Sun, 21 Jun 2020 22:02:00: 22000000 INFO @ Sun, 21 Jun 2020 22:02:04: 26000000 INFO @ Sun, 21 Jun 2020 22:02:05: 29000000 INFO @ Sun, 21 Jun 2020 22:02:09: 23000000 INFO @ Sun, 21 Jun 2020 22:02:12: 27000000 INFO @ Sun, 21 Jun 2020 22:02:14: 30000000 INFO @ Sun, 21 Jun 2020 22:02:17: 24000000 INFO @ Sun, 21 Jun 2020 22:02:21: 28000000 INFO @ Sun, 21 Jun 2020 22:02:23: 31000000 INFO @ Sun, 21 Jun 2020 22:02:26: 25000000 INFO @ Sun, 21 Jun 2020 22:02:30: 29000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:02:32: 32000000 INFO @ Sun, 21 Jun 2020 22:02:35: 26000000 INFO @ Sun, 21 Jun 2020 22:02:38: 30000000 INFO @ Sun, 21 Jun 2020 22:02:41: 33000000 INFO @ Sun, 21 Jun 2020 22:02:43: 27000000 INFO @ Sun, 21 Jun 2020 22:02:47: 31000000 INFO @ Sun, 21 Jun 2020 22:02:51: 34000000 INFO @ Sun, 21 Jun 2020 22:02:52: 28000000 INFO @ Sun, 21 Jun 2020 22:02:55: 32000000 INFO @ Sun, 21 Jun 2020 22:03:00: 35000000 INFO @ Sun, 21 Jun 2020 22:03:00: 29000000 INFO @ Sun, 21 Jun 2020 22:03:04: 33000000 INFO @ Sun, 21 Jun 2020 22:03:09: 30000000 INFO @ Sun, 21 Jun 2020 22:03:10: 36000000 INFO @ Sun, 21 Jun 2020 22:03:13: 34000000 INFO @ Sun, 21 Jun 2020 22:03:18: 31000000 INFO @ Sun, 21 Jun 2020 22:03:20: 37000000 INFO @ Sun, 21 Jun 2020 22:03:22: 35000000 INFO @ Sun, 21 Jun 2020 22:03:26: 32000000 INFO @ Sun, 21 Jun 2020 22:03:29: 38000000 INFO @ Sun, 21 Jun 2020 22:03:30: 36000000 INFO @ Sun, 21 Jun 2020 22:03:35: 33000000 INFO @ Sun, 21 Jun 2020 22:03:39: 39000000 INFO @ Sun, 21 Jun 2020 22:03:39: 37000000 INFO @ Sun, 21 Jun 2020 22:03:44: 34000000 INFO @ Sun, 21 Jun 2020 22:03:48: 38000000 INFO @ Sun, 21 Jun 2020 22:03:48: 40000000 INFO @ Sun, 21 Jun 2020 22:03:53: 35000000 INFO @ Sun, 21 Jun 2020 22:03:57: 39000000 INFO @ Sun, 21 Jun 2020 22:03:58: 41000000 INFO @ Sun, 21 Jun 2020 22:04:02: 36000000 INFO @ Sun, 21 Jun 2020 22:04:03: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:04:03: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:04:03: #1 total tags in treatment: 41445293 INFO @ Sun, 21 Jun 2020 22:04:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:04:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:04:04: #1 tags after filtering in treatment: 41445156 INFO @ Sun, 21 Jun 2020 22:04:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:04:04: #1 finished! INFO @ Sun, 21 Jun 2020 22:04:04: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:04:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:04:05: 40000000 INFO @ Sun, 21 Jun 2020 22:04:06: #2 number of paired peaks: 65 WARNING @ Sun, 21 Jun 2020 22:04:06: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:04:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:04:10: 37000000 INFO @ Sun, 21 Jun 2020 22:04:14: 41000000 INFO @ Sun, 21 Jun 2020 22:04:18: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:04:18: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:04:18: #1 total tags in treatment: 41445293 INFO @ Sun, 21 Jun 2020 22:04:18: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:04:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:04:18: 38000000 INFO @ Sun, 21 Jun 2020 22:04:19: #1 tags after filtering in treatment: 41445156 INFO @ Sun, 21 Jun 2020 22:04:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:04:19: #1 finished! INFO @ Sun, 21 Jun 2020 22:04:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:04:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:04:21: #2 number of paired peaks: 65 WARNING @ Sun, 21 Jun 2020 22:04:21: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:04:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:04:26: 39000000 INFO @ Sun, 21 Jun 2020 22:04:33: 40000000 INFO @ Sun, 21 Jun 2020 22:04:40: 41000000 INFO @ Sun, 21 Jun 2020 22:04:43: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:04:43: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:04:43: #1 total tags in treatment: 41445293 INFO @ Sun, 21 Jun 2020 22:04:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:04:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:04:44: #1 tags after filtering in treatment: 41445156 INFO @ Sun, 21 Jun 2020 22:04:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:04:44: #1 finished! INFO @ Sun, 21 Jun 2020 22:04:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:04:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:04:47: #2 number of paired peaks: 65 WARNING @ Sun, 21 Jun 2020 22:04:47: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:04:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370360/SRX370360.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling