Job ID = 6456604 SRX = SRX370359 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T11:07:24 prefetch.2.10.7: 1) Downloading 'SRR1020538'... 2020-06-21T11:07:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:50:43 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:50:43 prefetch.2.10.7: 1) 'SRR1020538' was downloaded successfully Read 142823755 spots for SRR1020538/SRR1020538.sra Written 142823755 spots for SRR1020538/SRR1020538.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:44:54 142823755 reads; of these: 142823755 (100.00%) were unpaired; of these: 31407504 (21.99%) aligned 0 times 101613922 (71.15%) aligned exactly 1 time 9802329 (6.86%) aligned >1 times 78.01% overall alignment rate Time searching: 00:44:55 Overall time: 00:44:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 62745594 / 111416251 = 0.5632 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:22:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:22:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:22:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:22:39: 1000000 INFO @ Sun, 21 Jun 2020 22:22:47: 2000000 INFO @ Sun, 21 Jun 2020 22:22:55: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:23:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:23:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:23:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:23:04: 4000000 INFO @ Sun, 21 Jun 2020 22:23:08: 1000000 INFO @ Sun, 21 Jun 2020 22:23:12: 5000000 INFO @ Sun, 21 Jun 2020 22:23:15: 2000000 INFO @ Sun, 21 Jun 2020 22:23:20: 6000000 INFO @ Sun, 21 Jun 2020 22:23:23: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:23:29: 7000000 INFO @ Sun, 21 Jun 2020 22:23:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:23:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:23:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:23:30: 4000000 INFO @ Sun, 21 Jun 2020 22:23:37: 8000000 INFO @ Sun, 21 Jun 2020 22:23:38: 5000000 INFO @ Sun, 21 Jun 2020 22:23:38: 1000000 INFO @ Sun, 21 Jun 2020 22:23:45: 9000000 INFO @ Sun, 21 Jun 2020 22:23:46: 6000000 INFO @ Sun, 21 Jun 2020 22:23:46: 2000000 INFO @ Sun, 21 Jun 2020 22:23:53: 7000000 INFO @ Sun, 21 Jun 2020 22:23:54: 10000000 INFO @ Sun, 21 Jun 2020 22:23:54: 3000000 INFO @ Sun, 21 Jun 2020 22:24:01: 8000000 INFO @ Sun, 21 Jun 2020 22:24:01: 11000000 INFO @ Sun, 21 Jun 2020 22:24:02: 4000000 INFO @ Sun, 21 Jun 2020 22:24:09: 9000000 INFO @ Sun, 21 Jun 2020 22:24:09: 12000000 INFO @ Sun, 21 Jun 2020 22:24:09: 5000000 INFO @ Sun, 21 Jun 2020 22:24:16: 10000000 INFO @ Sun, 21 Jun 2020 22:24:16: 13000000 INFO @ Sun, 21 Jun 2020 22:24:17: 6000000 INFO @ Sun, 21 Jun 2020 22:24:24: 11000000 INFO @ Sun, 21 Jun 2020 22:24:24: 14000000 INFO @ Sun, 21 Jun 2020 22:24:24: 7000000 INFO @ Sun, 21 Jun 2020 22:24:32: 12000000 INFO @ Sun, 21 Jun 2020 22:24:32: 15000000 INFO @ Sun, 21 Jun 2020 22:24:32: 8000000 INFO @ Sun, 21 Jun 2020 22:24:39: 13000000 INFO @ Sun, 21 Jun 2020 22:24:39: 16000000 INFO @ Sun, 21 Jun 2020 22:24:40: 9000000 INFO @ Sun, 21 Jun 2020 22:24:47: 14000000 INFO @ Sun, 21 Jun 2020 22:24:47: 17000000 INFO @ Sun, 21 Jun 2020 22:24:47: 10000000 INFO @ Sun, 21 Jun 2020 22:24:54: 18000000 INFO @ Sun, 21 Jun 2020 22:24:54: 15000000 INFO @ Sun, 21 Jun 2020 22:24:55: 11000000 INFO @ Sun, 21 Jun 2020 22:25:01: 19000000 INFO @ Sun, 21 Jun 2020 22:25:02: 16000000 INFO @ Sun, 21 Jun 2020 22:25:03: 12000000 INFO @ Sun, 21 Jun 2020 22:25:09: 20000000 INFO @ Sun, 21 Jun 2020 22:25:10: 17000000 INFO @ Sun, 21 Jun 2020 22:25:10: 13000000 INFO @ Sun, 21 Jun 2020 22:25:16: 21000000 INFO @ Sun, 21 Jun 2020 22:25:17: 18000000 INFO @ Sun, 21 Jun 2020 22:25:18: 14000000 INFO @ Sun, 21 Jun 2020 22:25:24: 22000000 INFO @ Sun, 21 Jun 2020 22:25:25: 19000000 INFO @ Sun, 21 Jun 2020 22:25:26: 15000000 INFO @ Sun, 21 Jun 2020 22:25:31: 23000000 INFO @ Sun, 21 Jun 2020 22:25:33: 20000000 INFO @ Sun, 21 Jun 2020 22:25:34: 16000000 INFO @ Sun, 21 Jun 2020 22:25:39: 24000000 INFO @ Sun, 21 Jun 2020 22:25:41: 21000000 INFO @ Sun, 21 Jun 2020 22:25:42: 17000000 INFO @ Sun, 21 Jun 2020 22:25:46: 25000000 INFO @ Sun, 21 Jun 2020 22:25:49: 22000000 INFO @ Sun, 21 Jun 2020 22:25:50: 18000000 INFO @ Sun, 21 Jun 2020 22:25:54: 26000000 INFO @ Sun, 21 Jun 2020 22:25:57: 23000000 INFO @ Sun, 21 Jun 2020 22:25:58: 19000000 INFO @ Sun, 21 Jun 2020 22:26:02: 27000000 INFO @ Sun, 21 Jun 2020 22:26:05: 24000000 INFO @ Sun, 21 Jun 2020 22:26:06: 20000000 INFO @ Sun, 21 Jun 2020 22:26:10: 28000000 INFO @ Sun, 21 Jun 2020 22:26:13: 25000000 INFO @ Sun, 21 Jun 2020 22:26:14: 21000000 INFO @ Sun, 21 Jun 2020 22:26:18: 29000000 INFO @ Sun, 21 Jun 2020 22:26:21: 26000000 INFO @ Sun, 21 Jun 2020 22:26:22: 22000000 INFO @ Sun, 21 Jun 2020 22:26:25: 30000000 INFO @ Sun, 21 Jun 2020 22:26:29: 27000000 INFO @ Sun, 21 Jun 2020 22:26:30: 23000000 INFO @ Sun, 21 Jun 2020 22:26:33: 31000000 INFO @ Sun, 21 Jun 2020 22:26:38: 28000000 INFO @ Sun, 21 Jun 2020 22:26:38: 24000000 INFO @ Sun, 21 Jun 2020 22:26:41: 32000000 INFO @ Sun, 21 Jun 2020 22:26:46: 29000000 INFO @ Sun, 21 Jun 2020 22:26:47: 25000000 INFO @ Sun, 21 Jun 2020 22:26:49: 33000000 INFO @ Sun, 21 Jun 2020 22:26:54: 30000000 INFO @ Sun, 21 Jun 2020 22:26:55: 26000000 INFO @ Sun, 21 Jun 2020 22:26:57: 34000000 INFO @ Sun, 21 Jun 2020 22:27:02: 31000000 INFO @ Sun, 21 Jun 2020 22:27:03: 27000000 INFO @ Sun, 21 Jun 2020 22:27:05: 35000000 INFO @ Sun, 21 Jun 2020 22:27:11: 32000000 INFO @ Sun, 21 Jun 2020 22:27:12: 28000000 INFO @ Sun, 21 Jun 2020 22:27:13: 36000000 INFO @ Sun, 21 Jun 2020 22:27:19: 33000000 INFO @ Sun, 21 Jun 2020 22:27:20: 29000000 INFO @ Sun, 21 Jun 2020 22:27:21: 37000000 INFO @ Sun, 21 Jun 2020 22:27:28: 34000000 INFO @ Sun, 21 Jun 2020 22:27:28: 30000000 INFO @ Sun, 21 Jun 2020 22:27:30: 38000000 INFO @ Sun, 21 Jun 2020 22:27:36: 35000000 INFO @ Sun, 21 Jun 2020 22:27:37: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:27:39: 39000000 INFO @ Sun, 21 Jun 2020 22:27:45: 36000000 INFO @ Sun, 21 Jun 2020 22:27:45: 32000000 INFO @ Sun, 21 Jun 2020 22:27:48: 40000000 INFO @ Sun, 21 Jun 2020 22:27:53: 37000000 INFO @ Sun, 21 Jun 2020 22:27:53: 33000000 INFO @ Sun, 21 Jun 2020 22:27:57: 41000000 INFO @ Sun, 21 Jun 2020 22:28:01: 38000000 INFO @ Sun, 21 Jun 2020 22:28:02: 34000000 INFO @ Sun, 21 Jun 2020 22:28:05: 42000000 INFO @ Sun, 21 Jun 2020 22:28:10: 39000000 INFO @ Sun, 21 Jun 2020 22:28:11: 35000000 INFO @ Sun, 21 Jun 2020 22:28:14: 43000000 INFO @ Sun, 21 Jun 2020 22:28:19: 40000000 INFO @ Sun, 21 Jun 2020 22:28:20: 36000000 INFO @ Sun, 21 Jun 2020 22:28:23: 44000000 INFO @ Sun, 21 Jun 2020 22:28:28: 41000000 INFO @ Sun, 21 Jun 2020 22:28:28: 37000000 INFO @ Sun, 21 Jun 2020 22:28:31: 45000000 INFO @ Sun, 21 Jun 2020 22:28:36: 42000000 INFO @ Sun, 21 Jun 2020 22:28:37: 38000000 INFO @ Sun, 21 Jun 2020 22:28:40: 46000000 INFO @ Sun, 21 Jun 2020 22:28:45: 43000000 INFO @ Sun, 21 Jun 2020 22:28:45: 39000000 INFO @ Sun, 21 Jun 2020 22:28:48: 47000000 INFO @ Sun, 21 Jun 2020 22:28:53: 44000000 INFO @ Sun, 21 Jun 2020 22:28:54: 40000000 INFO @ Sun, 21 Jun 2020 22:28:57: 48000000 INFO @ Sun, 21 Jun 2020 22:29:02: 45000000 INFO @ Sun, 21 Jun 2020 22:29:03: 41000000 INFO @ Sun, 21 Jun 2020 22:29:04: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:29:04: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:29:04: #1 total tags in treatment: 48670657 INFO @ Sun, 21 Jun 2020 22:29:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:29:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:29:05: #1 tags after filtering in treatment: 48670528 INFO @ Sun, 21 Jun 2020 22:29:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:29:05: #1 finished! INFO @ Sun, 21 Jun 2020 22:29:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:29:05: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:29:08: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:29:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:29:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:29:10: 46000000 INFO @ Sun, 21 Jun 2020 22:29:11: 42000000 INFO @ Sun, 21 Jun 2020 22:29:18: 47000000 INFO @ Sun, 21 Jun 2020 22:29:20: 43000000 INFO @ Sun, 21 Jun 2020 22:29:27: 48000000 INFO @ Sun, 21 Jun 2020 22:29:28: 44000000 INFO @ Sun, 21 Jun 2020 22:29:33: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:29:33: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:29:33: #1 total tags in treatment: 48670657 INFO @ Sun, 21 Jun 2020 22:29:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:29:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:29:35: #1 tags after filtering in treatment: 48670528 INFO @ Sun, 21 Jun 2020 22:29:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:29:35: #1 finished! INFO @ Sun, 21 Jun 2020 22:29:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:29:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:29:37: 45000000 INFO @ Sun, 21 Jun 2020 22:29:38: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:29:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:29:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:29:45: 46000000 INFO @ Sun, 21 Jun 2020 22:29:54: 47000000 INFO @ Sun, 21 Jun 2020 22:30:02: 48000000 INFO @ Sun, 21 Jun 2020 22:30:09: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:30:09: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:30:09: #1 total tags in treatment: 48670657 INFO @ Sun, 21 Jun 2020 22:30:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:30:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:30:10: #1 tags after filtering in treatment: 48670528 INFO @ Sun, 21 Jun 2020 22:30:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:30:10: #1 finished! INFO @ Sun, 21 Jun 2020 22:30:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:30:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:30:13: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:30:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:30:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX370359/SRX370359.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling