Job ID = 6456534 SRX = SRX3630331 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:53:16 prefetch.2.10.7: 1) Downloading 'SRR6652853'... 2020-06-21T10:53:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:01:43 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:01:43 prefetch.2.10.7: 1) 'SRR6652853' was downloaded successfully 2020-06-21T11:01:43 prefetch.2.10.7: 'SRR6652853' has 0 unresolved dependencies Read 79827204 spots for SRR6652853/SRR6652853.sra Written 79827204 spots for SRR6652853/SRR6652853.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:38 79827204 reads; of these: 79827204 (100.00%) were unpaired; of these: 4028711 (5.05%) aligned 0 times 57848737 (72.47%) aligned exactly 1 time 17949756 (22.49%) aligned >1 times 94.95% overall alignment rate Time searching: 00:23:38 Overall time: 00:23:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 19922212 / 75798493 = 0.2628 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:46:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:46:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:46:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:46:20: 1000000 INFO @ Sun, 21 Jun 2020 20:46:25: 2000000 INFO @ Sun, 21 Jun 2020 20:46:31: 3000000 INFO @ Sun, 21 Jun 2020 20:46:37: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:46:43: 5000000 INFO @ Sun, 21 Jun 2020 20:46:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:46:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:46:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:46:48: 6000000 INFO @ Sun, 21 Jun 2020 20:46:49: 1000000 INFO @ Sun, 21 Jun 2020 20:46:54: 7000000 INFO @ Sun, 21 Jun 2020 20:46:55: 2000000 INFO @ Sun, 21 Jun 2020 20:47:00: 8000000 INFO @ Sun, 21 Jun 2020 20:47:01: 3000000 INFO @ Sun, 21 Jun 2020 20:47:06: 9000000 INFO @ Sun, 21 Jun 2020 20:47:06: 4000000 INFO @ Sun, 21 Jun 2020 20:47:12: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:47:12: 5000000 INFO @ Sun, 21 Jun 2020 20:47:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:47:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:47:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:47:18: 11000000 INFO @ Sun, 21 Jun 2020 20:47:18: 6000000 INFO @ Sun, 21 Jun 2020 20:47:20: 1000000 INFO @ Sun, 21 Jun 2020 20:47:23: 12000000 INFO @ Sun, 21 Jun 2020 20:47:24: 7000000 INFO @ Sun, 21 Jun 2020 20:47:26: 2000000 INFO @ Sun, 21 Jun 2020 20:47:29: 13000000 INFO @ Sun, 21 Jun 2020 20:47:30: 8000000 INFO @ Sun, 21 Jun 2020 20:47:32: 3000000 INFO @ Sun, 21 Jun 2020 20:47:35: 14000000 INFO @ Sun, 21 Jun 2020 20:47:36: 9000000 INFO @ Sun, 21 Jun 2020 20:47:38: 4000000 INFO @ Sun, 21 Jun 2020 20:47:41: 15000000 INFO @ Sun, 21 Jun 2020 20:47:42: 10000000 INFO @ Sun, 21 Jun 2020 20:47:44: 5000000 INFO @ Sun, 21 Jun 2020 20:47:47: 16000000 INFO @ Sun, 21 Jun 2020 20:47:48: 11000000 INFO @ Sun, 21 Jun 2020 20:47:50: 6000000 INFO @ Sun, 21 Jun 2020 20:47:52: 17000000 INFO @ Sun, 21 Jun 2020 20:47:54: 12000000 INFO @ Sun, 21 Jun 2020 20:47:56: 7000000 INFO @ Sun, 21 Jun 2020 20:47:58: 18000000 INFO @ Sun, 21 Jun 2020 20:48:00: 13000000 INFO @ Sun, 21 Jun 2020 20:48:02: 8000000 INFO @ Sun, 21 Jun 2020 20:48:04: 19000000 INFO @ Sun, 21 Jun 2020 20:48:06: 14000000 INFO @ Sun, 21 Jun 2020 20:48:08: 9000000 INFO @ Sun, 21 Jun 2020 20:48:10: 20000000 INFO @ Sun, 21 Jun 2020 20:48:12: 15000000 INFO @ Sun, 21 Jun 2020 20:48:14: 10000000 INFO @ Sun, 21 Jun 2020 20:48:15: 21000000 INFO @ Sun, 21 Jun 2020 20:48:18: 16000000 INFO @ Sun, 21 Jun 2020 20:48:20: 11000000 INFO @ Sun, 21 Jun 2020 20:48:21: 22000000 INFO @ Sun, 21 Jun 2020 20:48:23: 17000000 INFO @ Sun, 21 Jun 2020 20:48:26: 12000000 INFO @ Sun, 21 Jun 2020 20:48:27: 23000000 INFO @ Sun, 21 Jun 2020 20:48:29: 18000000 INFO @ Sun, 21 Jun 2020 20:48:32: 13000000 INFO @ Sun, 21 Jun 2020 20:48:33: 24000000 INFO @ Sun, 21 Jun 2020 20:48:35: 19000000 INFO @ Sun, 21 Jun 2020 20:48:38: 14000000 INFO @ Sun, 21 Jun 2020 20:48:39: 25000000 INFO @ Sun, 21 Jun 2020 20:48:41: 20000000 INFO @ Sun, 21 Jun 2020 20:48:44: 15000000 INFO @ Sun, 21 Jun 2020 20:48:44: 26000000 INFO @ Sun, 21 Jun 2020 20:48:46: 21000000 INFO @ Sun, 21 Jun 2020 20:48:49: 16000000 INFO @ Sun, 21 Jun 2020 20:48:50: 27000000 INFO @ Sun, 21 Jun 2020 20:48:52: 22000000 INFO @ Sun, 21 Jun 2020 20:48:55: 17000000 INFO @ Sun, 21 Jun 2020 20:48:56: 28000000 INFO @ Sun, 21 Jun 2020 20:48:58: 23000000 INFO @ Sun, 21 Jun 2020 20:49:01: 18000000 INFO @ Sun, 21 Jun 2020 20:49:01: 29000000 INFO @ Sun, 21 Jun 2020 20:49:04: 24000000 INFO @ Sun, 21 Jun 2020 20:49:07: 19000000 INFO @ Sun, 21 Jun 2020 20:49:07: 30000000 INFO @ Sun, 21 Jun 2020 20:49:10: 25000000 INFO @ Sun, 21 Jun 2020 20:49:12: 31000000 INFO @ Sun, 21 Jun 2020 20:49:13: 20000000 INFO @ Sun, 21 Jun 2020 20:49:16: 26000000 INFO @ Sun, 21 Jun 2020 20:49:18: 32000000 INFO @ Sun, 21 Jun 2020 20:49:18: 21000000 INFO @ Sun, 21 Jun 2020 20:49:21: 27000000 INFO @ Sun, 21 Jun 2020 20:49:24: 33000000 INFO @ Sun, 21 Jun 2020 20:49:24: 22000000 INFO @ Sun, 21 Jun 2020 20:49:27: 28000000 INFO @ Sun, 21 Jun 2020 20:49:29: 34000000 INFO @ Sun, 21 Jun 2020 20:49:30: 23000000 INFO @ Sun, 21 Jun 2020 20:49:33: 29000000 INFO @ Sun, 21 Jun 2020 20:49:35: 35000000 INFO @ Sun, 21 Jun 2020 20:49:36: 24000000 INFO @ Sun, 21 Jun 2020 20:49:38: 30000000 INFO @ Sun, 21 Jun 2020 20:49:40: 36000000 INFO @ Sun, 21 Jun 2020 20:49:42: 25000000 INFO @ Sun, 21 Jun 2020 20:49:44: 31000000 INFO @ Sun, 21 Jun 2020 20:49:46: 37000000 INFO @ Sun, 21 Jun 2020 20:49:47: 26000000 INFO @ Sun, 21 Jun 2020 20:49:50: 32000000 INFO @ Sun, 21 Jun 2020 20:49:52: 38000000 INFO @ Sun, 21 Jun 2020 20:49:53: 27000000 INFO @ Sun, 21 Jun 2020 20:49:55: 33000000 INFO @ Sun, 21 Jun 2020 20:49:57: 39000000 INFO @ Sun, 21 Jun 2020 20:49:59: 28000000 INFO @ Sun, 21 Jun 2020 20:50:01: 34000000 INFO @ Sun, 21 Jun 2020 20:50:03: 40000000 INFO @ Sun, 21 Jun 2020 20:50:05: 29000000 INFO @ Sun, 21 Jun 2020 20:50:07: 35000000 INFO @ Sun, 21 Jun 2020 20:50:09: 41000000 INFO @ Sun, 21 Jun 2020 20:50:11: 30000000 INFO @ Sun, 21 Jun 2020 20:50:13: 36000000 INFO @ Sun, 21 Jun 2020 20:50:14: 42000000 INFO @ Sun, 21 Jun 2020 20:50:16: 31000000 INFO @ Sun, 21 Jun 2020 20:50:19: 37000000 INFO @ Sun, 21 Jun 2020 20:50:20: 43000000 INFO @ Sun, 21 Jun 2020 20:50:22: 32000000 INFO @ Sun, 21 Jun 2020 20:50:25: 38000000 INFO @ Sun, 21 Jun 2020 20:50:26: 44000000 INFO @ Sun, 21 Jun 2020 20:50:28: 33000000 INFO @ Sun, 21 Jun 2020 20:50:31: 39000000 INFO @ Sun, 21 Jun 2020 20:50:32: 45000000 INFO @ Sun, 21 Jun 2020 20:50:34: 34000000 INFO @ Sun, 21 Jun 2020 20:50:36: 40000000 INFO @ Sun, 21 Jun 2020 20:50:37: 46000000 INFO @ Sun, 21 Jun 2020 20:50:39: 35000000 INFO @ Sun, 21 Jun 2020 20:50:42: 41000000 INFO @ Sun, 21 Jun 2020 20:50:43: 47000000 INFO @ Sun, 21 Jun 2020 20:50:45: 36000000 INFO @ Sun, 21 Jun 2020 20:50:48: 42000000 INFO @ Sun, 21 Jun 2020 20:50:49: 48000000 INFO @ Sun, 21 Jun 2020 20:50:51: 37000000 INFO @ Sun, 21 Jun 2020 20:50:54: 43000000 INFO @ Sun, 21 Jun 2020 20:50:54: 49000000 INFO @ Sun, 21 Jun 2020 20:50:57: 38000000 INFO @ Sun, 21 Jun 2020 20:51:00: 44000000 INFO @ Sun, 21 Jun 2020 20:51:00: 50000000 INFO @ Sun, 21 Jun 2020 20:51:03: 39000000 INFO @ Sun, 21 Jun 2020 20:51:06: 51000000 INFO @ Sun, 21 Jun 2020 20:51:06: 45000000 INFO @ Sun, 21 Jun 2020 20:51:09: 40000000 INFO @ Sun, 21 Jun 2020 20:51:11: 52000000 INFO @ Sun, 21 Jun 2020 20:51:11: 46000000 INFO @ Sun, 21 Jun 2020 20:51:15: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:51:17: 53000000 INFO @ Sun, 21 Jun 2020 20:51:17: 47000000 INFO @ Sun, 21 Jun 2020 20:51:21: 42000000 INFO @ Sun, 21 Jun 2020 20:51:23: 54000000 INFO @ Sun, 21 Jun 2020 20:51:23: 48000000 INFO @ Sun, 21 Jun 2020 20:51:26: 43000000 INFO @ Sun, 21 Jun 2020 20:51:29: 55000000 INFO @ Sun, 21 Jun 2020 20:51:29: 49000000 INFO @ Sun, 21 Jun 2020 20:51:33: 44000000 INFO @ Sun, 21 Jun 2020 20:51:35: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:51:35: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:51:35: #1 total tags in treatment: 55876281 INFO @ Sun, 21 Jun 2020 20:51:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:51:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:51:35: 50000000 INFO @ Sun, 21 Jun 2020 20:51:36: #1 tags after filtering in treatment: 55876259 INFO @ Sun, 21 Jun 2020 20:51:36: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:51:36: #1 finished! INFO @ Sun, 21 Jun 2020 20:51:36: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:51:36: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:51:39: 45000000 INFO @ Sun, 21 Jun 2020 20:51:39: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:51:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:51:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:51:41: 51000000 INFO @ Sun, 21 Jun 2020 20:51:44: 46000000 INFO @ Sun, 21 Jun 2020 20:51:47: 52000000 INFO @ Sun, 21 Jun 2020 20:51:50: 47000000 INFO @ Sun, 21 Jun 2020 20:51:52: 53000000 INFO @ Sun, 21 Jun 2020 20:51:56: 48000000 INFO @ Sun, 21 Jun 2020 20:51:58: 54000000 INFO @ Sun, 21 Jun 2020 20:52:02: 49000000 INFO @ Sun, 21 Jun 2020 20:52:04: 55000000 INFO @ Sun, 21 Jun 2020 20:52:08: 50000000 INFO @ Sun, 21 Jun 2020 20:52:10: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:52:10: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:52:10: #1 total tags in treatment: 55876281 INFO @ Sun, 21 Jun 2020 20:52:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:52:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:52:11: #1 tags after filtering in treatment: 55876259 INFO @ Sun, 21 Jun 2020 20:52:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:52:11: #1 finished! INFO @ Sun, 21 Jun 2020 20:52:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:52:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:52:14: 51000000 INFO @ Sun, 21 Jun 2020 20:52:14: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:52:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:52:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:52:19: 52000000 INFO @ Sun, 21 Jun 2020 20:52:25: 53000000 INFO @ Sun, 21 Jun 2020 20:52:31: 54000000 INFO @ Sun, 21 Jun 2020 20:52:37: 55000000 INFO @ Sun, 21 Jun 2020 20:52:42: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:52:42: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:52:42: #1 total tags in treatment: 55876281 INFO @ Sun, 21 Jun 2020 20:52:42: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:52:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:52:44: #1 tags after filtering in treatment: 55876259 INFO @ Sun, 21 Jun 2020 20:52:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:52:44: #1 finished! INFO @ Sun, 21 Jun 2020 20:52:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:52:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:52:47: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:52:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:52:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630331/SRX3630331.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。