Job ID = 6456530 SRX = SRX3630329 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:58:31 prefetch.2.10.7: 1) Downloading 'SRR6652851'... 2020-06-21T10:58:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:05:47 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:05:47 prefetch.2.10.7: 1) 'SRR6652851' was downloaded successfully 2020-06-21T11:05:47 prefetch.2.10.7: 'SRR6652851' has 0 unresolved dependencies Read 74853287 spots for SRR6652851/SRR6652851.sra Written 74853287 spots for SRR6652851/SRR6652851.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:21:50 74853287 reads; of these: 74853287 (100.00%) were unpaired; of these: 3834694 (5.12%) aligned 0 times 55756048 (74.49%) aligned exactly 1 time 15262545 (20.39%) aligned >1 times 94.88% overall alignment rate Time searching: 00:21:51 Overall time: 00:21:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 21946210 / 71018593 = 0.3090 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:49:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:49:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:49:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:49:07: 1000000 INFO @ Sun, 21 Jun 2020 20:49:13: 2000000 INFO @ Sun, 21 Jun 2020 20:49:18: 3000000 INFO @ Sun, 21 Jun 2020 20:49:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:49:30: 5000000 INFO @ Sun, 21 Jun 2020 20:49:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:49:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:49:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:49:36: 6000000 INFO @ Sun, 21 Jun 2020 20:49:38: 1000000 INFO @ Sun, 21 Jun 2020 20:49:42: 7000000 INFO @ Sun, 21 Jun 2020 20:49:44: 2000000 INFO @ Sun, 21 Jun 2020 20:49:49: 8000000 INFO @ Sun, 21 Jun 2020 20:49:50: 3000000 INFO @ Sun, 21 Jun 2020 20:49:55: 9000000 INFO @ Sun, 21 Jun 2020 20:49:57: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:50:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:50:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:50:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:50:02: 10000000 INFO @ Sun, 21 Jun 2020 20:50:03: 5000000 INFO @ Sun, 21 Jun 2020 20:50:08: 1000000 INFO @ Sun, 21 Jun 2020 20:50:08: 11000000 INFO @ Sun, 21 Jun 2020 20:50:10: 6000000 INFO @ Sun, 21 Jun 2020 20:50:15: 12000000 INFO @ Sun, 21 Jun 2020 20:50:15: 2000000 INFO @ Sun, 21 Jun 2020 20:50:17: 7000000 INFO @ Sun, 21 Jun 2020 20:50:22: 13000000 INFO @ Sun, 21 Jun 2020 20:50:22: 3000000 INFO @ Sun, 21 Jun 2020 20:50:24: 8000000 INFO @ Sun, 21 Jun 2020 20:50:29: 14000000 INFO @ Sun, 21 Jun 2020 20:50:29: 4000000 INFO @ Sun, 21 Jun 2020 20:50:31: 9000000 INFO @ Sun, 21 Jun 2020 20:50:35: 15000000 INFO @ Sun, 21 Jun 2020 20:50:36: 5000000 INFO @ Sun, 21 Jun 2020 20:50:38: 10000000 INFO @ Sun, 21 Jun 2020 20:50:42: 16000000 INFO @ Sun, 21 Jun 2020 20:50:43: 6000000 INFO @ Sun, 21 Jun 2020 20:50:45: 11000000 INFO @ Sun, 21 Jun 2020 20:50:49: 17000000 INFO @ Sun, 21 Jun 2020 20:50:49: 7000000 INFO @ Sun, 21 Jun 2020 20:50:52: 12000000 INFO @ Sun, 21 Jun 2020 20:50:56: 18000000 INFO @ Sun, 21 Jun 2020 20:50:56: 8000000 INFO @ Sun, 21 Jun 2020 20:50:58: 13000000 INFO @ Sun, 21 Jun 2020 20:51:03: 19000000 INFO @ Sun, 21 Jun 2020 20:51:03: 9000000 INFO @ Sun, 21 Jun 2020 20:51:05: 14000000 INFO @ Sun, 21 Jun 2020 20:51:09: 20000000 INFO @ Sun, 21 Jun 2020 20:51:10: 10000000 INFO @ Sun, 21 Jun 2020 20:51:12: 15000000 INFO @ Sun, 21 Jun 2020 20:51:16: 21000000 INFO @ Sun, 21 Jun 2020 20:51:17: 11000000 INFO @ Sun, 21 Jun 2020 20:51:19: 16000000 INFO @ Sun, 21 Jun 2020 20:51:22: 22000000 INFO @ Sun, 21 Jun 2020 20:51:23: 12000000 INFO @ Sun, 21 Jun 2020 20:51:25: 17000000 INFO @ Sun, 21 Jun 2020 20:51:29: 23000000 INFO @ Sun, 21 Jun 2020 20:51:30: 13000000 INFO @ Sun, 21 Jun 2020 20:51:32: 18000000 INFO @ Sun, 21 Jun 2020 20:51:36: 24000000 INFO @ Sun, 21 Jun 2020 20:51:37: 14000000 INFO @ Sun, 21 Jun 2020 20:51:39: 19000000 INFO @ Sun, 21 Jun 2020 20:51:42: 25000000 INFO @ Sun, 21 Jun 2020 20:51:43: 15000000 INFO @ Sun, 21 Jun 2020 20:51:45: 20000000 INFO @ Sun, 21 Jun 2020 20:51:48: 26000000 INFO @ Sun, 21 Jun 2020 20:51:50: 16000000 INFO @ Sun, 21 Jun 2020 20:51:52: 21000000 INFO @ Sun, 21 Jun 2020 20:51:55: 27000000 INFO @ Sun, 21 Jun 2020 20:51:57: 17000000 INFO @ Sun, 21 Jun 2020 20:51:59: 22000000 INFO @ Sun, 21 Jun 2020 20:52:02: 28000000 INFO @ Sun, 21 Jun 2020 20:52:03: 18000000 INFO @ Sun, 21 Jun 2020 20:52:05: 23000000 INFO @ Sun, 21 Jun 2020 20:52:08: 29000000 INFO @ Sun, 21 Jun 2020 20:52:10: 19000000 INFO @ Sun, 21 Jun 2020 20:52:12: 24000000 INFO @ Sun, 21 Jun 2020 20:52:14: 30000000 INFO @ Sun, 21 Jun 2020 20:52:17: 20000000 INFO @ Sun, 21 Jun 2020 20:52:19: 25000000 INFO @ Sun, 21 Jun 2020 20:52:21: 31000000 INFO @ Sun, 21 Jun 2020 20:52:23: 21000000 INFO @ Sun, 21 Jun 2020 20:52:25: 26000000 INFO @ Sun, 21 Jun 2020 20:52:27: 32000000 INFO @ Sun, 21 Jun 2020 20:52:30: 22000000 INFO @ Sun, 21 Jun 2020 20:52:32: 27000000 INFO @ Sun, 21 Jun 2020 20:52:34: 33000000 INFO @ Sun, 21 Jun 2020 20:52:36: 23000000 INFO @ Sun, 21 Jun 2020 20:52:39: 28000000 INFO @ Sun, 21 Jun 2020 20:52:40: 34000000 INFO @ Sun, 21 Jun 2020 20:52:43: 24000000 INFO @ Sun, 21 Jun 2020 20:52:45: 29000000 INFO @ Sun, 21 Jun 2020 20:52:46: 35000000 INFO @ Sun, 21 Jun 2020 20:52:50: 25000000 INFO @ Sun, 21 Jun 2020 20:52:52: 30000000 INFO @ Sun, 21 Jun 2020 20:52:53: 36000000 INFO @ Sun, 21 Jun 2020 20:52:56: 26000000 INFO @ Sun, 21 Jun 2020 20:52:58: 31000000 INFO @ Sun, 21 Jun 2020 20:52:59: 37000000 INFO @ Sun, 21 Jun 2020 20:53:03: 27000000 INFO @ Sun, 21 Jun 2020 20:53:05: 32000000 INFO @ Sun, 21 Jun 2020 20:53:06: 38000000 INFO @ Sun, 21 Jun 2020 20:53:10: 28000000 INFO @ Sun, 21 Jun 2020 20:53:11: 33000000 INFO @ Sun, 21 Jun 2020 20:53:13: 39000000 INFO @ Sun, 21 Jun 2020 20:53:16: 29000000 INFO @ Sun, 21 Jun 2020 20:53:18: 34000000 INFO @ Sun, 21 Jun 2020 20:53:19: 40000000 INFO @ Sun, 21 Jun 2020 20:53:22: 30000000 INFO @ Sun, 21 Jun 2020 20:53:24: 35000000 INFO @ Sun, 21 Jun 2020 20:53:25: 41000000 INFO @ Sun, 21 Jun 2020 20:53:29: 31000000 INFO @ Sun, 21 Jun 2020 20:53:31: 36000000 INFO @ Sun, 21 Jun 2020 20:53:32: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:53:35: 32000000 INFO @ Sun, 21 Jun 2020 20:53:37: 37000000 INFO @ Sun, 21 Jun 2020 20:53:38: 43000000 INFO @ Sun, 21 Jun 2020 20:53:41: 33000000 INFO @ Sun, 21 Jun 2020 20:53:44: 38000000 INFO @ Sun, 21 Jun 2020 20:53:44: 44000000 INFO @ Sun, 21 Jun 2020 20:53:48: 34000000 INFO @ Sun, 21 Jun 2020 20:53:51: 45000000 INFO @ Sun, 21 Jun 2020 20:53:51: 39000000 INFO @ Sun, 21 Jun 2020 20:53:54: 35000000 INFO @ Sun, 21 Jun 2020 20:53:57: 46000000 INFO @ Sun, 21 Jun 2020 20:53:57: 40000000 INFO @ Sun, 21 Jun 2020 20:54:01: 36000000 INFO @ Sun, 21 Jun 2020 20:54:03: 47000000 INFO @ Sun, 21 Jun 2020 20:54:03: 41000000 INFO @ Sun, 21 Jun 2020 20:54:07: 37000000 INFO @ Sun, 21 Jun 2020 20:54:09: 48000000 INFO @ Sun, 21 Jun 2020 20:54:10: 42000000 INFO @ Sun, 21 Jun 2020 20:54:13: 38000000 INFO @ Sun, 21 Jun 2020 20:54:16: 49000000 INFO @ Sun, 21 Jun 2020 20:54:16: 43000000 INFO @ Sun, 21 Jun 2020 20:54:17: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:54:17: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:54:17: #1 total tags in treatment: 49072383 INFO @ Sun, 21 Jun 2020 20:54:17: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:54:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:54:18: #1 tags after filtering in treatment: 49072337 INFO @ Sun, 21 Jun 2020 20:54:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:54:18: #1 finished! INFO @ Sun, 21 Jun 2020 20:54:18: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:54:18: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:54:20: 39000000 INFO @ Sun, 21 Jun 2020 20:54:21: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:54:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:54:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:54:22: 44000000 INFO @ Sun, 21 Jun 2020 20:54:26: 40000000 INFO @ Sun, 21 Jun 2020 20:54:28: 45000000 INFO @ Sun, 21 Jun 2020 20:54:32: 41000000 INFO @ Sun, 21 Jun 2020 20:54:35: 46000000 INFO @ Sun, 21 Jun 2020 20:54:38: 42000000 INFO @ Sun, 21 Jun 2020 20:54:41: 47000000 INFO @ Sun, 21 Jun 2020 20:54:44: 43000000 INFO @ Sun, 21 Jun 2020 20:54:47: 48000000 INFO @ Sun, 21 Jun 2020 20:54:50: 44000000 INFO @ Sun, 21 Jun 2020 20:54:53: 49000000 INFO @ Sun, 21 Jun 2020 20:54:54: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:54:54: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:54:54: #1 total tags in treatment: 49072383 INFO @ Sun, 21 Jun 2020 20:54:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:54:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:54:55: #1 tags after filtering in treatment: 49072337 INFO @ Sun, 21 Jun 2020 20:54:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:54:55: #1 finished! INFO @ Sun, 21 Jun 2020 20:54:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:54:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:54:56: 45000000 INFO @ Sun, 21 Jun 2020 20:54:58: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:54:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:54:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:55:02: 46000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 20:55:08: 47000000 INFO @ Sun, 21 Jun 2020 20:55:13: 48000000 INFO @ Sun, 21 Jun 2020 20:55:19: 49000000 INFO @ Sun, 21 Jun 2020 20:55:20: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:55:20: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:55:20: #1 total tags in treatment: 49072383 INFO @ Sun, 21 Jun 2020 20:55:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:55:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:55:21: #1 tags after filtering in treatment: 49072337 INFO @ Sun, 21 Jun 2020 20:55:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:55:21: #1 finished! INFO @ Sun, 21 Jun 2020 20:55:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:55:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:55:24: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:55:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:55:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630329/SRX3630329.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling