Job ID = 6456528 SRX = SRX3630327 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:55:31 prefetch.2.10.7: 1) Downloading 'SRR6652849'... 2020-06-21T10:55:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:04:25 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:04:25 prefetch.2.10.7: 1) 'SRR6652849' was downloaded successfully 2020-06-21T11:04:25 prefetch.2.10.7: 'SRR6652849' has 0 unresolved dependencies Read 88666841 spots for SRR6652849/SRR6652849.sra Written 88666841 spots for SRR6652849/SRR6652849.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:53 88666841 reads; of these: 88666841 (100.00%) were unpaired; of these: 6964888 (7.86%) aligned 0 times 61550521 (69.42%) aligned exactly 1 time 20151432 (22.73%) aligned >1 times 92.14% overall alignment rate Time searching: 00:26:53 Overall time: 00:26:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 21215049 / 81701953 = 0.2597 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:53:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:53:22: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:53:22: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:53:27: 1000000 INFO @ Sun, 21 Jun 2020 20:53:32: 2000000 INFO @ Sun, 21 Jun 2020 20:53:37: 3000000 INFO @ Sun, 21 Jun 2020 20:53:42: 4000000 INFO @ Sun, 21 Jun 2020 20:53:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:53:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:53:52: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:53:52: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:53:52: 6000000 INFO @ Sun, 21 Jun 2020 20:53:58: 7000000 INFO @ Sun, 21 Jun 2020 20:53:59: 1000000 INFO @ Sun, 21 Jun 2020 20:54:04: 8000000 INFO @ Sun, 21 Jun 2020 20:54:05: 2000000 INFO @ Sun, 21 Jun 2020 20:54:10: 9000000 INFO @ Sun, 21 Jun 2020 20:54:11: 3000000 INFO @ Sun, 21 Jun 2020 20:54:15: 10000000 INFO @ Sun, 21 Jun 2020 20:54:17: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:54:21: 11000000 INFO @ Sun, 21 Jun 2020 20:54:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:54:22: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:54:22: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:54:24: 5000000 INFO @ Sun, 21 Jun 2020 20:54:27: 12000000 INFO @ Sun, 21 Jun 2020 20:54:28: 1000000 INFO @ Sun, 21 Jun 2020 20:54:30: 6000000 INFO @ Sun, 21 Jun 2020 20:54:32: 13000000 INFO @ Sun, 21 Jun 2020 20:54:34: 2000000 INFO @ Sun, 21 Jun 2020 20:54:36: 7000000 INFO @ Sun, 21 Jun 2020 20:54:38: 14000000 INFO @ Sun, 21 Jun 2020 20:54:40: 3000000 INFO @ Sun, 21 Jun 2020 20:54:42: 8000000 INFO @ Sun, 21 Jun 2020 20:54:44: 15000000 INFO @ Sun, 21 Jun 2020 20:54:45: 4000000 INFO @ Sun, 21 Jun 2020 20:54:49: 9000000 INFO @ Sun, 21 Jun 2020 20:54:50: 16000000 INFO @ Sun, 21 Jun 2020 20:54:51: 5000000 INFO @ Sun, 21 Jun 2020 20:54:55: 10000000 INFO @ Sun, 21 Jun 2020 20:54:56: 17000000 INFO @ Sun, 21 Jun 2020 20:54:57: 6000000 INFO @ Sun, 21 Jun 2020 20:55:01: 11000000 INFO @ Sun, 21 Jun 2020 20:55:01: 18000000 INFO @ Sun, 21 Jun 2020 20:55:03: 7000000 INFO @ Sun, 21 Jun 2020 20:55:07: 19000000 INFO @ Sun, 21 Jun 2020 20:55:07: 12000000 INFO @ Sun, 21 Jun 2020 20:55:09: 8000000 INFO @ Sun, 21 Jun 2020 20:55:13: 20000000 INFO @ Sun, 21 Jun 2020 20:55:14: 13000000 INFO @ Sun, 21 Jun 2020 20:55:14: 9000000 INFO @ Sun, 21 Jun 2020 20:55:19: 21000000 INFO @ Sun, 21 Jun 2020 20:55:20: 14000000 INFO @ Sun, 21 Jun 2020 20:55:20: 10000000 INFO @ Sun, 21 Jun 2020 20:55:25: 22000000 INFO @ Sun, 21 Jun 2020 20:55:26: 11000000 INFO @ Sun, 21 Jun 2020 20:55:26: 15000000 INFO @ Sun, 21 Jun 2020 20:55:30: 23000000 INFO @ Sun, 21 Jun 2020 20:55:32: 12000000 INFO @ Sun, 21 Jun 2020 20:55:32: 16000000 INFO @ Sun, 21 Jun 2020 20:55:36: 24000000 INFO @ Sun, 21 Jun 2020 20:55:37: 13000000 INFO @ Sun, 21 Jun 2020 20:55:39: 17000000 INFO @ Sun, 21 Jun 2020 20:55:42: 25000000 INFO @ Sun, 21 Jun 2020 20:55:43: 14000000 INFO @ Sun, 21 Jun 2020 20:55:45: 18000000 INFO @ Sun, 21 Jun 2020 20:55:47: 26000000 INFO @ Sun, 21 Jun 2020 20:55:49: 15000000 INFO @ Sun, 21 Jun 2020 20:55:52: 19000000 INFO @ Sun, 21 Jun 2020 20:55:53: 27000000 INFO @ Sun, 21 Jun 2020 20:55:55: 16000000 INFO @ Sun, 21 Jun 2020 20:55:58: 20000000 INFO @ Sun, 21 Jun 2020 20:55:59: 28000000 INFO @ Sun, 21 Jun 2020 20:56:00: 17000000 INFO @ Sun, 21 Jun 2020 20:56:04: 21000000 INFO @ Sun, 21 Jun 2020 20:56:04: 29000000 INFO @ Sun, 21 Jun 2020 20:56:06: 18000000 INFO @ Sun, 21 Jun 2020 20:56:10: 30000000 INFO @ Sun, 21 Jun 2020 20:56:11: 22000000 INFO @ Sun, 21 Jun 2020 20:56:12: 19000000 INFO @ Sun, 21 Jun 2020 20:56:15: 31000000 INFO @ Sun, 21 Jun 2020 20:56:17: 23000000 INFO @ Sun, 21 Jun 2020 20:56:18: 20000000 INFO @ Sun, 21 Jun 2020 20:56:21: 32000000 INFO @ Sun, 21 Jun 2020 20:56:23: 24000000 INFO @ Sun, 21 Jun 2020 20:56:23: 21000000 INFO @ Sun, 21 Jun 2020 20:56:27: 33000000 INFO @ Sun, 21 Jun 2020 20:56:29: 25000000 INFO @ Sun, 21 Jun 2020 20:56:29: 22000000 INFO @ Sun, 21 Jun 2020 20:56:32: 34000000 INFO @ Sun, 21 Jun 2020 20:56:35: 23000000 INFO @ Sun, 21 Jun 2020 20:56:35: 26000000 INFO @ Sun, 21 Jun 2020 20:56:38: 35000000 INFO @ Sun, 21 Jun 2020 20:56:41: 24000000 INFO @ Sun, 21 Jun 2020 20:56:41: 27000000 INFO @ Sun, 21 Jun 2020 20:56:44: 36000000 INFO @ Sun, 21 Jun 2020 20:56:46: 25000000 INFO @ Sun, 21 Jun 2020 20:56:48: 28000000 INFO @ Sun, 21 Jun 2020 20:56:49: 37000000 INFO @ Sun, 21 Jun 2020 20:56:52: 26000000 INFO @ Sun, 21 Jun 2020 20:56:54: 29000000 INFO @ Sun, 21 Jun 2020 20:56:55: 38000000 INFO @ Sun, 21 Jun 2020 20:56:58: 27000000 INFO @ Sun, 21 Jun 2020 20:57:00: 30000000 INFO @ Sun, 21 Jun 2020 20:57:01: 39000000 INFO @ Sun, 21 Jun 2020 20:57:03: 28000000 INFO @ Sun, 21 Jun 2020 20:57:06: 31000000 INFO @ Sun, 21 Jun 2020 20:57:07: 40000000 INFO @ Sun, 21 Jun 2020 20:57:10: 29000000 INFO @ Sun, 21 Jun 2020 20:57:13: 32000000 INFO @ Sun, 21 Jun 2020 20:57:13: 41000000 INFO @ Sun, 21 Jun 2020 20:57:16: 30000000 INFO @ Sun, 21 Jun 2020 20:57:19: 33000000 INFO @ Sun, 21 Jun 2020 20:57:19: 42000000 INFO @ Sun, 21 Jun 2020 20:57:22: 31000000 INFO @ Sun, 21 Jun 2020 20:57:25: 34000000 INFO @ Sun, 21 Jun 2020 20:57:25: 43000000 INFO @ Sun, 21 Jun 2020 20:57:28: 32000000 INFO @ Sun, 21 Jun 2020 20:57:32: 35000000 INFO @ Sun, 21 Jun 2020 20:57:32: 44000000 INFO @ Sun, 21 Jun 2020 20:57:33: 33000000 INFO @ Sun, 21 Jun 2020 20:57:38: 45000000 INFO @ Sun, 21 Jun 2020 20:57:38: 36000000 INFO @ Sun, 21 Jun 2020 20:57:39: 34000000 INFO @ Sun, 21 Jun 2020 20:57:44: 46000000 INFO @ Sun, 21 Jun 2020 20:57:44: 37000000 INFO @ Sun, 21 Jun 2020 20:57:45: 35000000 INFO @ Sun, 21 Jun 2020 20:57:50: 47000000 INFO @ Sun, 21 Jun 2020 20:57:50: 38000000 INFO @ Sun, 21 Jun 2020 20:57:51: 36000000 INFO @ Sun, 21 Jun 2020 20:57:56: 48000000 INFO @ Sun, 21 Jun 2020 20:57:56: 39000000 INFO @ Sun, 21 Jun 2020 20:57:57: 37000000 INFO @ Sun, 21 Jun 2020 20:58:01: 49000000 INFO @ Sun, 21 Jun 2020 20:58:03: 40000000 INFO @ Sun, 21 Jun 2020 20:58:03: 38000000 INFO @ Sun, 21 Jun 2020 20:58:07: 50000000 INFO @ Sun, 21 Jun 2020 20:58:09: 39000000 INFO @ Sun, 21 Jun 2020 20:58:09: 41000000 INFO @ Sun, 21 Jun 2020 20:58:13: 51000000 INFO @ Sun, 21 Jun 2020 20:58:15: 40000000 INFO @ Sun, 21 Jun 2020 20:58:15: 42000000 INFO @ Sun, 21 Jun 2020 20:58:19: 52000000 INFO @ Sun, 21 Jun 2020 20:58:20: 41000000 INFO @ Sun, 21 Jun 2020 20:58:22: 43000000 INFO @ Sun, 21 Jun 2020 20:58:24: 53000000 INFO @ Sun, 21 Jun 2020 20:58:26: 42000000 INFO @ Sun, 21 Jun 2020 20:58:28: 44000000 INFO @ Sun, 21 Jun 2020 20:58:30: 54000000 INFO @ Sun, 21 Jun 2020 20:58:32: 43000000 INFO @ Sun, 21 Jun 2020 20:58:35: 45000000 INFO @ Sun, 21 Jun 2020 20:58:36: 55000000 INFO @ Sun, 21 Jun 2020 20:58:38: 44000000 INFO @ Sun, 21 Jun 2020 20:58:41: 46000000 INFO @ Sun, 21 Jun 2020 20:58:42: 56000000 INFO @ Sun, 21 Jun 2020 20:58:44: 45000000 INFO @ Sun, 21 Jun 2020 20:58:47: 47000000 INFO @ Sun, 21 Jun 2020 20:58:48: 57000000 INFO @ Sun, 21 Jun 2020 20:58:49: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:58:54: 58000000 INFO @ Sun, 21 Jun 2020 20:58:54: 48000000 INFO @ Sun, 21 Jun 2020 20:58:55: 47000000 INFO @ Sun, 21 Jun 2020 20:58:59: 59000000 INFO @ Sun, 21 Jun 2020 20:59:00: 49000000 INFO @ Sun, 21 Jun 2020 20:59:01: 48000000 INFO @ Sun, 21 Jun 2020 20:59:05: 60000000 INFO @ Sun, 21 Jun 2020 20:59:07: 50000000 INFO @ Sun, 21 Jun 2020 20:59:07: 49000000 INFO @ Sun, 21 Jun 2020 20:59:08: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:59:08: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:59:08: #1 total tags in treatment: 60486904 INFO @ Sun, 21 Jun 2020 20:59:08: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:59:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:59:10: #1 tags after filtering in treatment: 60486872 INFO @ Sun, 21 Jun 2020 20:59:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:59:10: #1 finished! INFO @ Sun, 21 Jun 2020 20:59:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:59:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:59:13: 51000000 INFO @ Sun, 21 Jun 2020 20:59:13: 50000000 INFO @ Sun, 21 Jun 2020 20:59:13: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:59:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:59:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:59:19: 51000000 INFO @ Sun, 21 Jun 2020 20:59:19: 52000000 INFO @ Sun, 21 Jun 2020 20:59:24: 52000000 INFO @ Sun, 21 Jun 2020 20:59:26: 53000000 INFO @ Sun, 21 Jun 2020 20:59:30: 53000000 INFO @ Sun, 21 Jun 2020 20:59:32: 54000000 INFO @ Sun, 21 Jun 2020 20:59:36: 54000000 INFO @ Sun, 21 Jun 2020 20:59:38: 55000000 INFO @ Sun, 21 Jun 2020 20:59:41: 55000000 INFO @ Sun, 21 Jun 2020 20:59:44: 56000000 INFO @ Sun, 21 Jun 2020 20:59:47: 56000000 INFO @ Sun, 21 Jun 2020 20:59:51: 57000000 INFO @ Sun, 21 Jun 2020 20:59:53: 57000000 INFO @ Sun, 21 Jun 2020 20:59:57: 58000000 INFO @ Sun, 21 Jun 2020 20:59:58: 58000000 INFO @ Sun, 21 Jun 2020 21:00:03: 59000000 INFO @ Sun, 21 Jun 2020 21:00:04: 59000000 INFO @ Sun, 21 Jun 2020 21:00:09: 60000000 INFO @ Sun, 21 Jun 2020 21:00:10: 60000000 INFO @ Sun, 21 Jun 2020 21:00:13: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 21:00:13: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 21:00:13: #1 total tags in treatment: 60486904 INFO @ Sun, 21 Jun 2020 21:00:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:00:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:00:13: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 21:00:13: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 21:00:13: #1 total tags in treatment: 60486904 INFO @ Sun, 21 Jun 2020 21:00:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:00:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:00:14: #1 tags after filtering in treatment: 60486872 INFO @ Sun, 21 Jun 2020 21:00:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:00:14: #1 finished! INFO @ Sun, 21 Jun 2020 21:00:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:00:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:00:15: #1 tags after filtering in treatment: 60486872 INFO @ Sun, 21 Jun 2020 21:00:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:00:15: #1 finished! INFO @ Sun, 21 Jun 2020 21:00:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:00:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:00:17: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:00:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:00:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:00:18: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:00:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:00:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630327/SRX3630327.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。