Job ID = 6456526 SRX = SRX3630325 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:59:16 prefetch.2.10.7: 1) Downloading 'SRR6652847'... 2020-06-21T10:59:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T11:06:00 prefetch.2.10.7: HTTPS download succeed 2020-06-21T11:06:00 prefetch.2.10.7: 1) 'SRR6652847' was downloaded successfully 2020-06-21T11:06:00 prefetch.2.10.7: 'SRR6652847' has 0 unresolved dependencies Read 71263728 spots for SRR6652847/SRR6652847.sra Written 71263728 spots for SRR6652847/SRR6652847.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:53 71263728 reads; of these: 71263728 (100.00%) were unpaired; of these: 5067763 (7.11%) aligned 0 times 50078828 (70.27%) aligned exactly 1 time 16117137 (22.62%) aligned >1 times 92.89% overall alignment rate Time searching: 00:20:53 Overall time: 00:20:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 15510090 / 66195965 = 0.2343 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:50:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:50:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:50:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:50:08: 1000000 INFO @ Sun, 21 Jun 2020 20:50:15: 2000000 INFO @ Sun, 21 Jun 2020 20:50:23: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:50:30: 4000000 INFO @ Sun, 21 Jun 2020 20:50:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:50:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:50:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:50:38: 5000000 INFO @ Sun, 21 Jun 2020 20:50:39: 1000000 INFO @ Sun, 21 Jun 2020 20:50:45: 6000000 INFO @ Sun, 21 Jun 2020 20:50:46: 2000000 INFO @ Sun, 21 Jun 2020 20:50:53: 7000000 INFO @ Sun, 21 Jun 2020 20:50:54: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:51:01: 8000000 INFO @ Sun, 21 Jun 2020 20:51:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:51:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:51:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:51:02: 4000000 INFO @ Sun, 21 Jun 2020 20:51:07: 1000000 INFO @ Sun, 21 Jun 2020 20:51:08: 9000000 INFO @ Sun, 21 Jun 2020 20:51:09: 5000000 INFO @ Sun, 21 Jun 2020 20:51:13: 2000000 INFO @ Sun, 21 Jun 2020 20:51:16: 10000000 INFO @ Sun, 21 Jun 2020 20:51:17: 6000000 INFO @ Sun, 21 Jun 2020 20:51:20: 3000000 INFO @ Sun, 21 Jun 2020 20:51:24: 11000000 INFO @ Sun, 21 Jun 2020 20:51:25: 7000000 INFO @ Sun, 21 Jun 2020 20:51:26: 4000000 INFO @ Sun, 21 Jun 2020 20:51:32: 12000000 INFO @ Sun, 21 Jun 2020 20:51:32: 5000000 INFO @ Sun, 21 Jun 2020 20:51:33: 8000000 INFO @ Sun, 21 Jun 2020 20:51:39: 6000000 INFO @ Sun, 21 Jun 2020 20:51:40: 13000000 INFO @ Sun, 21 Jun 2020 20:51:40: 9000000 INFO @ Sun, 21 Jun 2020 20:51:45: 7000000 INFO @ Sun, 21 Jun 2020 20:51:47: 14000000 INFO @ Sun, 21 Jun 2020 20:51:48: 10000000 INFO @ Sun, 21 Jun 2020 20:51:51: 8000000 INFO @ Sun, 21 Jun 2020 20:51:55: 15000000 INFO @ Sun, 21 Jun 2020 20:51:56: 11000000 INFO @ Sun, 21 Jun 2020 20:51:57: 9000000 INFO @ Sun, 21 Jun 2020 20:52:03: 16000000 INFO @ Sun, 21 Jun 2020 20:52:04: 10000000 INFO @ Sun, 21 Jun 2020 20:52:04: 12000000 INFO @ Sun, 21 Jun 2020 20:52:10: 11000000 INFO @ Sun, 21 Jun 2020 20:52:10: 17000000 INFO @ Sun, 21 Jun 2020 20:52:11: 13000000 INFO @ Sun, 21 Jun 2020 20:52:16: 12000000 INFO @ Sun, 21 Jun 2020 20:52:18: 18000000 INFO @ Sun, 21 Jun 2020 20:52:19: 14000000 INFO @ Sun, 21 Jun 2020 20:52:22: 13000000 INFO @ Sun, 21 Jun 2020 20:52:26: 19000000 INFO @ Sun, 21 Jun 2020 20:52:27: 15000000 INFO @ Sun, 21 Jun 2020 20:52:29: 14000000 INFO @ Sun, 21 Jun 2020 20:52:34: 20000000 INFO @ Sun, 21 Jun 2020 20:52:35: 16000000 INFO @ Sun, 21 Jun 2020 20:52:36: 15000000 INFO @ Sun, 21 Jun 2020 20:52:41: 21000000 INFO @ Sun, 21 Jun 2020 20:52:42: 17000000 INFO @ Sun, 21 Jun 2020 20:52:42: 16000000 INFO @ Sun, 21 Jun 2020 20:52:49: 17000000 INFO @ Sun, 21 Jun 2020 20:52:49: 22000000 INFO @ Sun, 21 Jun 2020 20:52:49: 18000000 INFO @ Sun, 21 Jun 2020 20:52:55: 18000000 INFO @ Sun, 21 Jun 2020 20:52:56: 23000000 INFO @ Sun, 21 Jun 2020 20:52:57: 19000000 INFO @ Sun, 21 Jun 2020 20:53:01: 19000000 INFO @ Sun, 21 Jun 2020 20:53:04: 24000000 INFO @ Sun, 21 Jun 2020 20:53:05: 20000000 INFO @ Sun, 21 Jun 2020 20:53:08: 20000000 INFO @ Sun, 21 Jun 2020 20:53:12: 25000000 INFO @ Sun, 21 Jun 2020 20:53:13: 21000000 INFO @ Sun, 21 Jun 2020 20:53:14: 21000000 INFO @ Sun, 21 Jun 2020 20:53:20: 26000000 INFO @ Sun, 21 Jun 2020 20:53:20: 22000000 INFO @ Sun, 21 Jun 2020 20:53:21: 22000000 INFO @ Sun, 21 Jun 2020 20:53:27: 27000000 INFO @ Sun, 21 Jun 2020 20:53:28: 23000000 INFO @ Sun, 21 Jun 2020 20:53:28: 23000000 INFO @ Sun, 21 Jun 2020 20:53:35: 24000000 INFO @ Sun, 21 Jun 2020 20:53:35: 28000000 INFO @ Sun, 21 Jun 2020 20:53:36: 24000000 INFO @ Sun, 21 Jun 2020 20:53:41: 25000000 INFO @ Sun, 21 Jun 2020 20:53:43: 29000000 INFO @ Sun, 21 Jun 2020 20:53:43: 25000000 INFO @ Sun, 21 Jun 2020 20:53:48: 26000000 INFO @ Sun, 21 Jun 2020 20:53:50: 30000000 INFO @ Sun, 21 Jun 2020 20:53:51: 26000000 INFO @ Sun, 21 Jun 2020 20:53:54: 27000000 INFO @ Sun, 21 Jun 2020 20:53:58: 31000000 INFO @ Sun, 21 Jun 2020 20:53:59: 27000000 INFO @ Sun, 21 Jun 2020 20:54:00: 28000000 INFO @ Sun, 21 Jun 2020 20:54:05: 32000000 INFO @ Sun, 21 Jun 2020 20:54:07: 28000000 INFO @ Sun, 21 Jun 2020 20:54:07: 29000000 INFO @ Sun, 21 Jun 2020 20:54:12: 33000000 INFO @ Sun, 21 Jun 2020 20:54:13: 30000000 INFO @ Sun, 21 Jun 2020 20:54:14: 29000000 INFO @ Sun, 21 Jun 2020 20:54:19: 31000000 INFO @ Sun, 21 Jun 2020 20:54:20: 34000000 INFO @ Sun, 21 Jun 2020 20:54:21: 30000000 INFO @ Sun, 21 Jun 2020 20:54:26: 32000000 INFO @ Sun, 21 Jun 2020 20:54:27: 35000000 INFO @ Sun, 21 Jun 2020 20:54:29: 31000000 INFO @ Sun, 21 Jun 2020 20:54:32: 33000000 INFO @ Sun, 21 Jun 2020 20:54:35: 36000000 INFO @ Sun, 21 Jun 2020 20:54:36: 32000000 INFO @ Sun, 21 Jun 2020 20:54:38: 34000000 INFO @ Sun, 21 Jun 2020 20:54:42: 37000000 INFO @ Sun, 21 Jun 2020 20:54:43: 33000000 INFO @ Sun, 21 Jun 2020 20:54:45: 35000000 INFO @ Sun, 21 Jun 2020 20:54:50: 38000000 INFO @ Sun, 21 Jun 2020 20:54:51: 34000000 INFO @ Sun, 21 Jun 2020 20:54:51: 36000000 INFO @ Sun, 21 Jun 2020 20:54:57: 37000000 INFO @ Sun, 21 Jun 2020 20:54:58: 39000000 INFO @ Sun, 21 Jun 2020 20:54:58: 35000000 INFO @ Sun, 21 Jun 2020 20:55:04: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:55:05: 40000000 INFO @ Sun, 21 Jun 2020 20:55:06: 36000000 INFO @ Sun, 21 Jun 2020 20:55:11: 39000000 INFO @ Sun, 21 Jun 2020 20:55:13: 37000000 INFO @ Sun, 21 Jun 2020 20:55:14: 41000000 INFO @ Sun, 21 Jun 2020 20:55:17: 40000000 INFO @ Sun, 21 Jun 2020 20:55:21: 38000000 INFO @ Sun, 21 Jun 2020 20:55:21: 42000000 INFO @ Sun, 21 Jun 2020 20:55:24: 41000000 INFO @ Sun, 21 Jun 2020 20:55:28: 39000000 INFO @ Sun, 21 Jun 2020 20:55:29: 43000000 INFO @ Sun, 21 Jun 2020 20:55:31: 42000000 INFO @ Sun, 21 Jun 2020 20:55:36: 40000000 INFO @ Sun, 21 Jun 2020 20:55:36: 44000000 INFO @ Sun, 21 Jun 2020 20:55:37: 43000000 INFO @ Sun, 21 Jun 2020 20:55:44: 41000000 INFO @ Sun, 21 Jun 2020 20:55:44: 44000000 INFO @ Sun, 21 Jun 2020 20:55:44: 45000000 INFO @ Sun, 21 Jun 2020 20:55:50: 45000000 INFO @ Sun, 21 Jun 2020 20:55:51: 42000000 INFO @ Sun, 21 Jun 2020 20:55:52: 46000000 INFO @ Sun, 21 Jun 2020 20:55:57: 46000000 INFO @ Sun, 21 Jun 2020 20:55:58: 43000000 INFO @ Sun, 21 Jun 2020 20:56:00: 47000000 INFO @ Sun, 21 Jun 2020 20:56:04: 47000000 INFO @ Sun, 21 Jun 2020 20:56:05: 44000000 INFO @ Sun, 21 Jun 2020 20:56:07: 48000000 INFO @ Sun, 21 Jun 2020 20:56:10: 48000000 INFO @ Sun, 21 Jun 2020 20:56:13: 45000000 INFO @ Sun, 21 Jun 2020 20:56:14: 49000000 INFO @ Sun, 21 Jun 2020 20:56:17: 49000000 INFO @ Sun, 21 Jun 2020 20:56:20: 46000000 INFO @ Sun, 21 Jun 2020 20:56:22: 50000000 INFO @ Sun, 21 Jun 2020 20:56:24: 50000000 INFO @ Sun, 21 Jun 2020 20:56:27: 47000000 INFO @ Sun, 21 Jun 2020 20:56:28: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:56:28: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:56:28: #1 total tags in treatment: 50685875 INFO @ Sun, 21 Jun 2020 20:56:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:56:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:56:29: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:56:29: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:56:29: #1 total tags in treatment: 50685875 INFO @ Sun, 21 Jun 2020 20:56:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:56:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:56:29: #1 tags after filtering in treatment: 50685837 INFO @ Sun, 21 Jun 2020 20:56:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:56:29: #1 finished! INFO @ Sun, 21 Jun 2020 20:56:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:56:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:56:30: #1 tags after filtering in treatment: 50685837 INFO @ Sun, 21 Jun 2020 20:56:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:56:30: #1 finished! INFO @ Sun, 21 Jun 2020 20:56:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:56:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:56:32: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:56:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:56:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:56:34: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:56:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:56:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:56:34: 48000000 INFO @ Sun, 21 Jun 2020 20:56:41: 49000000 INFO @ Sun, 21 Jun 2020 20:56:48: 50000000 INFO @ Sun, 21 Jun 2020 20:56:54: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:56:54: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:56:54: #1 total tags in treatment: 50685875 INFO @ Sun, 21 Jun 2020 20:56:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:56:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:56:55: #1 tags after filtering in treatment: 50685837 INFO @ Sun, 21 Jun 2020 20:56:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:56:55: #1 finished! INFO @ Sun, 21 Jun 2020 20:56:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:56:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:56:58: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:56:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:56:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630325/SRX3630325.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。