Job ID = 6456524 SRX = SRX3630323 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:52:46 prefetch.2.10.7: 1) Downloading 'SRR6652845'... 2020-06-21T10:52:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:59:36 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:59:36 prefetch.2.10.7: 1) 'SRR6652845' was downloaded successfully 2020-06-21T10:59:36 prefetch.2.10.7: 'SRR6652845' has 0 unresolved dependencies Read 66689191 spots for SRR6652845/SRR6652845.sra Written 66689191 spots for SRR6652845/SRR6652845.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:45 66689191 reads; of these: 66689191 (100.00%) were unpaired; of these: 3620276 (5.43%) aligned 0 times 50884949 (76.30%) aligned exactly 1 time 12183966 (18.27%) aligned >1 times 94.57% overall alignment rate Time searching: 00:16:45 Overall time: 00:16:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 15548300 / 63068915 = 0.2465 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:34:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:34:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:34:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:34:23: 1000000 INFO @ Sun, 21 Jun 2020 20:34:28: 2000000 INFO @ Sun, 21 Jun 2020 20:34:33: 3000000 INFO @ Sun, 21 Jun 2020 20:34:38: 4000000 INFO @ Sun, 21 Jun 2020 20:34:43: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:34:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:34:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:34:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:34:48: 6000000 INFO @ Sun, 21 Jun 2020 20:34:53: 1000000 INFO @ Sun, 21 Jun 2020 20:34:54: 7000000 INFO @ Sun, 21 Jun 2020 20:34:59: 2000000 INFO @ Sun, 21 Jun 2020 20:34:59: 8000000 INFO @ Sun, 21 Jun 2020 20:35:04: 3000000 INFO @ Sun, 21 Jun 2020 20:35:04: 9000000 INFO @ Sun, 21 Jun 2020 20:35:10: 4000000 INFO @ Sun, 21 Jun 2020 20:35:10: 10000000 INFO @ Sun, 21 Jun 2020 20:35:15: 5000000 INFO @ Sun, 21 Jun 2020 20:35:15: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 20:35:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 20:35:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 20:35:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 20:35:20: 6000000 INFO @ Sun, 21 Jun 2020 20:35:20: 12000000 INFO @ Sun, 21 Jun 2020 20:35:24: 1000000 INFO @ Sun, 21 Jun 2020 20:35:26: 7000000 INFO @ Sun, 21 Jun 2020 20:35:26: 13000000 INFO @ Sun, 21 Jun 2020 20:35:29: 2000000 INFO @ Sun, 21 Jun 2020 20:35:31: 8000000 INFO @ Sun, 21 Jun 2020 20:35:32: 14000000 INFO @ Sun, 21 Jun 2020 20:35:34: 3000000 INFO @ Sun, 21 Jun 2020 20:35:37: 9000000 INFO @ Sun, 21 Jun 2020 20:35:37: 15000000 INFO @ Sun, 21 Jun 2020 20:35:40: 4000000 INFO @ Sun, 21 Jun 2020 20:35:42: 10000000 INFO @ Sun, 21 Jun 2020 20:35:42: 16000000 INFO @ Sun, 21 Jun 2020 20:35:45: 5000000 INFO @ Sun, 21 Jun 2020 20:35:47: 11000000 INFO @ Sun, 21 Jun 2020 20:35:48: 17000000 INFO @ Sun, 21 Jun 2020 20:35:51: 6000000 INFO @ Sun, 21 Jun 2020 20:35:53: 12000000 INFO @ Sun, 21 Jun 2020 20:35:53: 18000000 INFO @ Sun, 21 Jun 2020 20:35:56: 7000000 INFO @ Sun, 21 Jun 2020 20:35:59: 13000000 INFO @ Sun, 21 Jun 2020 20:35:59: 19000000 INFO @ Sun, 21 Jun 2020 20:36:02: 8000000 INFO @ Sun, 21 Jun 2020 20:36:04: 14000000 INFO @ Sun, 21 Jun 2020 20:36:04: 20000000 INFO @ Sun, 21 Jun 2020 20:36:07: 9000000 INFO @ Sun, 21 Jun 2020 20:36:10: 21000000 INFO @ Sun, 21 Jun 2020 20:36:10: 15000000 INFO @ Sun, 21 Jun 2020 20:36:13: 10000000 INFO @ Sun, 21 Jun 2020 20:36:15: 22000000 INFO @ Sun, 21 Jun 2020 20:36:15: 16000000 INFO @ Sun, 21 Jun 2020 20:36:18: 11000000 INFO @ Sun, 21 Jun 2020 20:36:21: 23000000 INFO @ Sun, 21 Jun 2020 20:36:21: 17000000 INFO @ Sun, 21 Jun 2020 20:36:24: 12000000 INFO @ Sun, 21 Jun 2020 20:36:26: 24000000 INFO @ Sun, 21 Jun 2020 20:36:26: 18000000 INFO @ Sun, 21 Jun 2020 20:36:29: 13000000 INFO @ Sun, 21 Jun 2020 20:36:32: 25000000 INFO @ Sun, 21 Jun 2020 20:36:32: 19000000 INFO @ Sun, 21 Jun 2020 20:36:35: 14000000 INFO @ Sun, 21 Jun 2020 20:36:37: 26000000 INFO @ Sun, 21 Jun 2020 20:36:37: 20000000 INFO @ Sun, 21 Jun 2020 20:36:41: 15000000 INFO @ Sun, 21 Jun 2020 20:36:43: 27000000 INFO @ Sun, 21 Jun 2020 20:36:43: 21000000 INFO @ Sun, 21 Jun 2020 20:36:46: 16000000 INFO @ Sun, 21 Jun 2020 20:36:48: 28000000 INFO @ Sun, 21 Jun 2020 20:36:49: 22000000 INFO @ Sun, 21 Jun 2020 20:36:51: 17000000 INFO @ Sun, 21 Jun 2020 20:36:54: 29000000 INFO @ Sun, 21 Jun 2020 20:36:54: 23000000 INFO @ Sun, 21 Jun 2020 20:36:57: 18000000 INFO @ Sun, 21 Jun 2020 20:37:00: 30000000 INFO @ Sun, 21 Jun 2020 20:37:00: 24000000 INFO @ Sun, 21 Jun 2020 20:37:02: 19000000 INFO @ Sun, 21 Jun 2020 20:37:05: 31000000 INFO @ Sun, 21 Jun 2020 20:37:05: 25000000 INFO @ Sun, 21 Jun 2020 20:37:08: 20000000 INFO @ Sun, 21 Jun 2020 20:37:11: 32000000 INFO @ Sun, 21 Jun 2020 20:37:11: 26000000 INFO @ Sun, 21 Jun 2020 20:37:13: 21000000 INFO @ Sun, 21 Jun 2020 20:37:17: 33000000 INFO @ Sun, 21 Jun 2020 20:37:17: 27000000 INFO @ Sun, 21 Jun 2020 20:37:19: 22000000 INFO @ Sun, 21 Jun 2020 20:37:22: 34000000 INFO @ Sun, 21 Jun 2020 20:37:23: 28000000 INFO @ Sun, 21 Jun 2020 20:37:25: 23000000 INFO @ Sun, 21 Jun 2020 20:37:28: 35000000 INFO @ Sun, 21 Jun 2020 20:37:28: 29000000 INFO @ Sun, 21 Jun 2020 20:37:30: 24000000 INFO @ Sun, 21 Jun 2020 20:37:34: 36000000 INFO @ Sun, 21 Jun 2020 20:37:34: 30000000 INFO @ Sun, 21 Jun 2020 20:37:36: 25000000 INFO @ Sun, 21 Jun 2020 20:37:40: 37000000 INFO @ Sun, 21 Jun 2020 20:37:40: 31000000 INFO @ Sun, 21 Jun 2020 20:37:42: 26000000 INFO @ Sun, 21 Jun 2020 20:37:46: 32000000 INFO @ Sun, 21 Jun 2020 20:37:46: 38000000 INFO @ Sun, 21 Jun 2020 20:37:47: 27000000 INFO @ Sun, 21 Jun 2020 20:37:52: 33000000 INFO @ Sun, 21 Jun 2020 20:37:52: 39000000 INFO @ Sun, 21 Jun 2020 20:37:53: 28000000 INFO @ Sun, 21 Jun 2020 20:37:57: 34000000 INFO @ Sun, 21 Jun 2020 20:37:58: 40000000 INFO @ Sun, 21 Jun 2020 20:37:59: 29000000 INFO @ Sun, 21 Jun 2020 20:38:03: 35000000 INFO @ Sun, 21 Jun 2020 20:38:03: 41000000 INFO @ Sun, 21 Jun 2020 20:38:05: 30000000 INFO @ Sun, 21 Jun 2020 20:38:09: 36000000 INFO @ Sun, 21 Jun 2020 20:38:09: 42000000 INFO @ Sun, 21 Jun 2020 20:38:10: 31000000 INFO @ Sun, 21 Jun 2020 20:38:15: 43000000 INFO @ Sun, 21 Jun 2020 20:38:15: 37000000 INFO @ Sun, 21 Jun 2020 20:38:16: 32000000 INFO @ Sun, 21 Jun 2020 20:38:21: 44000000 INFO @ Sun, 21 Jun 2020 20:38:21: 38000000 INFO @ Sun, 21 Jun 2020 20:38:22: 33000000 INFO @ Sun, 21 Jun 2020 20:38:26: 45000000 INFO @ Sun, 21 Jun 2020 20:38:27: 39000000 INFO @ Sun, 21 Jun 2020 20:38:28: 34000000 INFO @ Sun, 21 Jun 2020 20:38:32: 46000000 INFO @ Sun, 21 Jun 2020 20:38:33: 40000000 INFO @ Sun, 21 Jun 2020 20:38:33: 35000000 INFO @ Sun, 21 Jun 2020 20:38:38: 47000000 INFO @ Sun, 21 Jun 2020 20:38:39: 41000000 INFO @ Sun, 21 Jun 2020 20:38:39: 36000000 INFO @ Sun, 21 Jun 2020 20:38:42: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:38:42: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:38:42: #1 total tags in treatment: 47520615 INFO @ Sun, 21 Jun 2020 20:38:42: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:38:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:38:43: #1 tags after filtering in treatment: 47520572 INFO @ Sun, 21 Jun 2020 20:38:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:38:43: #1 finished! INFO @ Sun, 21 Jun 2020 20:38:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:38:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:38:44: 42000000 INFO @ Sun, 21 Jun 2020 20:38:45: 37000000 INFO @ Sun, 21 Jun 2020 20:38:46: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 20:38:46: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:38:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:38:50: 43000000 INFO @ Sun, 21 Jun 2020 20:38:51: 38000000 INFO @ Sun, 21 Jun 2020 20:38:56: 44000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:38:57: 39000000 INFO @ Sun, 21 Jun 2020 20:39:01: 45000000 INFO @ Sun, 21 Jun 2020 20:39:02: 40000000 INFO @ Sun, 21 Jun 2020 20:39:07: 46000000 INFO @ Sun, 21 Jun 2020 20:39:08: 41000000 INFO @ Sun, 21 Jun 2020 20:39:13: 47000000 INFO @ Sun, 21 Jun 2020 20:39:14: 42000000 INFO @ Sun, 21 Jun 2020 20:39:16: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:39:16: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:39:16: #1 total tags in treatment: 47520615 INFO @ Sun, 21 Jun 2020 20:39:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:39:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:39:17: #1 tags after filtering in treatment: 47520572 INFO @ Sun, 21 Jun 2020 20:39:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:39:17: #1 finished! INFO @ Sun, 21 Jun 2020 20:39:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:39:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:39:19: 43000000 INFO @ Sun, 21 Jun 2020 20:39:20: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 20:39:20: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:39:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:39:24: 44000000 INFO @ Sun, 21 Jun 2020 20:39:30: 45000000 INFO @ Sun, 21 Jun 2020 20:39:35: 46000000 INFO @ Sun, 21 Jun 2020 20:39:41: 47000000 INFO @ Sun, 21 Jun 2020 20:39:44: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 20:39:44: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 20:39:44: #1 total tags in treatment: 47520615 INFO @ Sun, 21 Jun 2020 20:39:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:39:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:39:45: #1 tags after filtering in treatment: 47520572 INFO @ Sun, 21 Jun 2020 20:39:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:39:45: #1 finished! INFO @ Sun, 21 Jun 2020 20:39:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:39:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:39:48: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 20:39:48: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:39:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX3630323/SRX3630323.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。